Latex enhanced turbidimetric immunoassay kit of quantitatively detecting procalcitonin PCT

Abstract

The invention relates to a latex enhanced turbidimetric immunoassay kit of quantitatively detecting procalcitonin PCT. The kit comprises an R1 reagent, an R2 reagent and a calibrator, wherein the R1 reagent comprises a protecting agent, a reaction enhancing agent, a preservative and buffer solution; the R2 reagent comprises a protecting agent, a preservative, buffer solution and anti-human PCT antibody coated sensitization polystyrene latex particles; the calibrator comprises a protecting agent, a preservative, buffer solution and PCT recombinant protein; the human PCT antibody in the R2 reagent is linked with polystyrene latex particles through streptavidin-biotin; and the particle diameter of the latex particles in the R2 reagent is 40-500 nm. The kit can be used on a biochemical analyzer and a scatter turbidimetry analyzer for quantitatively detecting the PCT content in human blood. The invention provides the PCT detection kit which has the advantages of convenience, quickness, high sensitivity, strong specificity and accurate quantification; and the kit has high instrument compatibility, is low in detection cost and meets the requirements on PCT turbidimetric products in clinical use.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit for quantitatively detecting procalcitonin PCT in human blood by using latex-enhanced immune turbidimetry. Background technique [0002] Procalcitonin (PCT) is the precursor substance of calcitonin CT, which is composed of 116 amino acids and has a molecular weight of 13kD. It is composed of three parts: N-terminal-calcitonin-C-terminal. The influence of hormone levels, the half-life in the human body is 25-30 hours, and has good stability. The main site of PCT production in the body is the liver, and other neuroendocrine cells such as peripheral blood mononuclear cells, spleen, lung or small intestine are also important sites for producing PCT. Endotoxin, cytokines can stimulate the release of PCT. [0003] As a clinical marker, PCT has unique advantages. The content of PCT in the blood of healthy people is extremely low (<0.05ng / ml), and 0.5ng / ml is con...

AI Technical Summary

Problems solved by technology

At present, the detection limit of various latex immunoturbidimetric detection products on the market is mostly limited to 0.1 μg / ml, and this detection limit is far from enough for the clinical application of procalcitonin turbidimetric products. Procalcitonin 0.5ng / ml is the cut-off value for the diagnosis of systemic infection (sepsis), and the PCT concentration in patients with lower respiratory tract bacterial infection may be lower than this cut-off value (≥0.25ng / ml and <0.5ng / ml), so the development sensitivity is higher PCT immunoturbidimetric products with stronger specificity, better stability and stronger anti-interference ability are still important problems to be solved in the field of clinical diagnostic product research

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