87 results about "Turbidimetric Immunoassays" patented technology

The invention relates to a kit for determining heart-type fatty acid binding protein in serum or urine by latex enhanced turbidimetric immunoassay. Specifically, the kit for determining the heart-type fatty acid binding protein comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 contains a reaction promoter, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; the reagent R2 contains latex particles with binding of anti-heart-type fatty acid binding protein monoclonal antibody and polyclonal antibody, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; and the calibrator contains an antiseptic, an electrolyte, a stabilizing agent, a heart-type fatty acid binding protein pure product and a buffer. By the complex coating method of latex particles with the monoclonal antibody and the polyclonal antibody, high sensitivity and wide linear range of the kit are guaranteed. Simultaneously, the kit also has advantages of high accuracy, good repeatability, strong singularity, easy operation and the like, and is applicable to an automatic biochemical analyzer which is commonly used in clinic.

The invention relates to a latex enhanced turbidimetric immunoassay kit of quantitatively detecting procalcitonin PCT. The kit comprises an R1 reagent, an R2 reagent and a calibrator, wherein the R1 reagent comprises a protecting agent, a reaction enhancing agent, a preservative and buffer solution; the R2 reagent comprises a protecting agent, a preservative, buffer solution and anti-human PCT antibody coated sensitization polystyrene latex particles; the calibrator comprises a protecting agent, a preservative, buffer solution and PCT recombinant protein; the human PCT antibody in the R2 reagent is linked with polystyrene latex particles through streptavidin-biotin; and the particle diameter of the latex particles in the R2 reagent is 40-500 nm. The kit can be used on a biochemical analyzer and a scatter turbidimetry analyzer for quantitatively detecting the PCT content in human blood. The invention provides the PCT detection kit which has the advantages of convenience, quickness, high sensitivity, strong specificity and accurate quantification; and the kit has high instrument compatibility, is low in detection cost and meets the requirements on PCT turbidimetric products in clinical use.

According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and / or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and / or minimum value is observed, and turbidity level.

The invention relates to a kit for determining myeloperoxidase (MPO) content in serum. The invention aims to solve the technical problem to overcome the defects in the background art and provides a kit for determining myeloperoxidase content by enhanced turbidimetric immunoassay, which has the advantages of no need of dilution of a sample, simplicity in operation, high accuracy, good repeatability and suitability and is applied to various full-automatic biochemical analyzers and various special protein instruments. The invention adopts a technical scheme that the kit for determining the myeloperoxidase content by enhanced turbidimetric immunoassay comprises the following components: a, a reagent R1 which comprises buffer solution, a preservative, an accelerating agent, inorganic salt, a surface active agenet and the balance of purified water; b, a reagent R2 which comprises buffer agent, antibody combined with anti-human myeloperoxidase, a preservative, wherein the diameter of latex microspheres is 60-150nm; c, a reference calibration material which comprises buffer solution, a stabilizing agent, a preservative, a recombinant human myeloperoxidase pure product in a certain amount determined by concentration requirement and the balance of purified water. By the reagent combination, the calibration curve of MPO content is established, thereby achieving rapid determination of the MPO content in the serum on the full-automatic biochemical analyzer or the special protein instrument.

There is a demand for improved turbidimetric immunoassays for human Cystatin C in biological samples, especially in human clinical samples of body fluids. The present invention provides a turbidimetric immunoassay method and reagent set enabling measurement of human Cystatin C by turbidimetric methods, resulting in a surprisingly stronger and faster turbidimetric signal than in the present state of the art. The increased and faster signal is accomplished by the use of new reagents and compositions, and enables shorter assay times and kinetic reading with a stronger signal, improving overall assay speed and quality. Improved robustness to lipid interference and improved linearity is achieved.

There is a demand for improved turbidimetric immunoassays for human Cystatin C in biological samples, especially in human clinical samples of body fluids. The present invention provides a turbidimetric immunoassay method and reagent set enabling measurement of human Cystatin C by turbidimetric methods, resulting in a surprisingly stronger and faster turbidimetric signal than in the present state of the art. The increased and faster signal is accomplished by the use of new reagents and compositions, and enables shorter assay times and kinetic reading with a stronger signal, improving overall assay speed and quality. Improved robustness to lipid interference and improved linearity is achieved.

The invention discloses a kit and a method for detecting the concentration of Kappa free light chains. The kit comprises a reaction buffer solution and a Kappa free light chain antibody solution, wherein the concentration of sodium chloride in the reaction buffer solution is 1250-2400 mmol / L. According to the kit, the problem of poor clinical specimen linearity in a turbidimetry assay of the Kappa free light chains is solved, reliable Kappa free light chain detection results as diagnostic references are provided for clinical doctors, the advantages of high speed and flux of a latex-enhanced turbidimetric immunoassay can be conveniently brought into full play, the detection time is shortened, and the clinical application and patient diagnosis are facilitated.

The invention discloses an alpha 1-microglobulin detection kit. The alpha 1-microglobulin detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution; the reagent R2 is a mixture of an alpha 1-microglobulin antibody-sensitization polystyrene latex particles and the buffer solution. The alpha 1-microglobulin detection kit disclosed by the invention has the advantages that 1, the detection is simple and quick, the sensitivity is high, the accuracy is good, the anti-jamming capacity is strong, and the production cost is low; 2, an alpha 1-microglobulin detection method adopted by the invention is a latex-enhanced turbidimetric immunoassay method, so that the alpha 1-microglobulin detection method enables the detection of alpha 1-microglobulin to be more economic, more convenient and quicker, is suitable for an automatic biochemical analyzer in most hospitals, and quick quantitative detection on emergency treatment can be specially realized.