87 results about "Turbidimetric Immunoassays" patented technology

The invention relates to a kit for determining heart-type fatty acid binding protein in serum or urine by latex enhanced turbidimetric immunoassay. Specifically, the kit for determining the heart-type fatty acid binding protein comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 contains a reaction promoter, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; the reagent R2 contains latex particles with binding of anti-heart-type fatty acid binding protein monoclonal antibody and polyclonal antibody, an antiseptic, a surfactant, a stabilizing agent, an electrolyte and a buffer; and the calibrator contains an antiseptic, an electrolyte, a stabilizing agent, a heart-type fatty acid binding protein pure product and a buffer. By the complex coating method of latex particles with the monoclonal antibody and the polyclonal antibody, high sensitivity and wide linear range of the kit are guaranteed. Simultaneously, the kit also has advantages of high accuracy, good repeatability, strong singularity, easy operation and the like, and is applicable to an automatic biochemical analyzer which is commonly used in clinic.

The invention relates to a latex enhanced turbidimetric immunoassay kit of quantitatively detecting procalcitonin PCT. The kit comprises an R1 reagent, an R2 reagent and a calibrator, wherein the R1 reagent comprises a protecting agent, a reaction enhancing agent, a preservative and buffer solution; the R2 reagent comprises a protecting agent, a preservative, buffer solution and anti-human PCT antibody coated sensitization polystyrene latex particles; the calibrator comprises a protecting agent, a preservative, buffer solution and PCT recombinant protein; the human PCT antibody in the R2 reagent is linked with polystyrene latex particles through streptavidin-biotin; and the particle diameter of the latex particles in the R2 reagent is 40-500 nm. The kit can be used on a biochemical analyzer and a scatter turbidimetry analyzer for quantitatively detecting the PCT content in human blood. The invention provides the PCT detection kit which has the advantages of convenience, quickness, high sensitivity, strong specificity and accurate quantification; and the kit has high instrument compatibility, is low in detection cost and meets the requirements on PCT turbidimetric products in clinical use.

The invention discloses a latex enhanced turbidimetric immunoassay kit for diagnosing gastric diseases or gastric cancer, a preparation method thereof and application. Components of the kit include diluent, blank solution and a latex reagent of an antibody of pepsinogen I or an antibody of pepsinogen II and can further include a calibrator and quality control serum. The latex reagent contains nanoparticles coupled with the antibody of the pepsinogen I or the antibody of the pepsinogen II, and the particle sizes of the nanoparticles are different. The examination sensitivity cannot meet requirements when latex with the single particle size lower than 100nm is used for examination, the linear range is small when latex with the single particle size of about 200nm is used for examination, and accordingly the examination sensitivity and the linear range cannot meet requirements when latex with the single particle size is used for examination. After composite latex of the kit is used for marking, the examination sensitivity can be improved, the linear examination range can be broadened, and the latex enhanced turbidimetric immunoassay kit has the advantages of fast examination, high sensitivity and specificity, good accuracy and the like in terms of gastric disease or gastric cancer examination.

The invention discloses a preparation method and application of D-dimer immuno latex microspheres. In the preparation method of D-dimer immuno latex microspheres, a chemical crosslinking method is optimized, the carboxyl of polystyrene microsphere is activated in a low-pH condition and then coupled with amino of an antibody dissolved in a high-pH condition, and the mixture of the two is neutral and is coupled for 12-24h at a low temperature, so that not only can the coupling efficiency of the antibody be guaranteed, but also the damage of the antibody activity caused by an activator is reduced to a great extent. A latex enhanced turbidimetric immunoassay D-dimer test kit prepared from the immuno latex microspheres prepared by the method has the characteristics of high sensitivity, accurate quantification, good repeatability and stable property, and the lowest detection limit can reach 0.01mg / L; the latex enhanced turbidimetric immunoassay D-dimer test kit can be applied to a full-automatic biochemical analyzer or coagulation analyzer, is quick and easy to operate, only needs 5-10 minutes from detection to result collection and has relatively good clinical application prospect.

The invention provides a kit for detecting serum amyloid protein and application thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and interference elimination protein; the reagent R2 is prepared from the buffer solution, the inorganic salt, the surfactant, the preservative, the stabilizer and a polystyrene latex particle mixture; the polystyrene latex particle mixture is cross-linked with an SAA (Serum Amyloid A) antibody; the polystyrene latex particle mixture is a mixture of large-diameter polystyrene latex particles and small-diameter polystyrene latex particles. The kit disclosed by the invention is based on PETIA (Particle-enhanced Turbidimetric Immunoassay) and can be generally used for analysis of all kinds of full-automatic biochemical analyzer; during use, the required determining time is short, the specificity is high, the precision degree is high, and the accuracy degree is high.

The invention provides a latex enhanced turbidimetric immunoassay kit for quantitatively detecting cystatin C. The kit comprises a reagent R1 and a reagent R2 which are independent from each other, wherein the reagent R1 is mainly prepared from a buffer solution 1, a stabilizing agent 1, a preservative 1, EDTA, accelerator and a protective agent 1; the reagent R2 is mainly prepared from polystyrene latex microspheres for crosslinking a cystatin C antibody, a buffer solution 2, a stabilizing 2, a preservative 2 and a protective agent 2, wherein the polystyrene latex microspheres and the cystatin C antibody are connected in a covalent cross-linking mode. The detection kit has the advantages of low preparation cost, good stability, good data repeatability and high detection sensitivity, is easy to store, and can be widely applied to clinical biochemical analyzers.

According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and / or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and / or minimum value is observed, and turbidity level.

The invention relates to a kit for determining myeloperoxidase (MPO) content in serum. The invention aims to solve the technical problem to overcome the defects in the background art and provides a kit for determining myeloperoxidase content by enhanced turbidimetric immunoassay, which has the advantages of no need of dilution of a sample, simplicity in operation, high accuracy, good repeatability and suitability and is applied to various full-automatic biochemical analyzers and various special protein instruments. The invention adopts a technical scheme that the kit for determining the myeloperoxidase content by enhanced turbidimetric immunoassay comprises the following components: a, a reagent R1 which comprises buffer solution, a preservative, an accelerating agent, inorganic salt, a surface active agenet and the balance of purified water; b, a reagent R2 which comprises buffer agent, antibody combined with anti-human myeloperoxidase, a preservative, wherein the diameter of latex microspheres is 60-150nm; c, a reference calibration material which comprises buffer solution, a stabilizing agent, a preservative, a recombinant human myeloperoxidase pure product in a certain amount determined by concentration requirement and the balance of purified water. By the reagent combination, the calibration curve of MPO content is established, thereby achieving rapid determination of the MPO content in the serum on the full-automatic biochemical analyzer or the special protein instrument.

The invention discloses a KL-6 determination reagent. The KL-6 determination reagent comprises a pretreatment reagent, an antibody latex reagent and an adjusting reagent, wherein the pretreatment reagent 7.2-7.6 in pH value comprises a buffer agent, a signal enhancing agent and a preservative, the antibody latex reagent comprises a buffer agent, latex microspheres and a stabilizing agent, the latex microspheres 100-400 nm in diameter combine anti-human KL-6 antibodies, and the adjusting agent comprises quantitative KL-6. The invention further discloses a preparation method of the KL-6 determination reagent. The KL-6 determination reagent and the preparation method thereof have the advantages that through combination of the anti-human KL-6 antibodies and the latex microspheres, the content of KL-6 in human serum can be determined through a latex-enhanced turbidimetric immunoassay; the KL-6 determination reagent is simple and convenient to operate, high in accuracy degree and repeatability and suitable for high-throughput test, can be used on a fully-automatic biochemical analyzer and well conforms to results determined by an enzyme-linked immunosorbent assay method.

There is a demand for improved turbidimetric immunoassays for human Cystatin C in biological samples, especially in human clinical samples of body fluids. The present invention provides a turbidimetric immunoassay method and reagent set enabling measurement of human Cystatin C by turbidimetric methods, resulting in a surprisingly stronger and faster turbidimetric signal than in the present state of the art. The increased and faster signal is accomplished by the use of new reagents and compositions, and enables shorter assay times and kinetic reading with a stronger signal, improving overall assay speed and quality. Improved robustness to lipid interference and improved linearity is achieved.

The invention provides a kit for determining Lp-PLA2 based on latex particle-enhanced turbidimetric immunoassay and a preparation method of the kit. The kit comprises reaction liquid and a latex antibody reagent, wherein the latex antibody reagent comprises at least two latex antibodies formed by coupling monoclonal antibodies with latex microspheres. The preparation method comprises the followingsteps: screening out Lp-PLA2 monoclonal antibodies with higher specificity, and mixing multiple monoclonal antibodies instead of polyclonal antibodies to carry out latex coupling to obtain the latexantibodies; then preparing the reaction liquid; finally, forming the kit by using the latex antibodies and the reaction liquid, wherein the kit is used for determining the content of the Lp-PLA2. By using the kit prepared by the preparation method provided by the invention, sensitivity and specificity of determining the Lp-PLA2 by the latex particle-enhanced turbidimetric immunoassay can be simultaneously improved; further, the kit has the advantages of wider linear range, higher accuracy, extremely-high economic value and broad market application prospect.

The invention provides a cystatin C latex-particle-enhanced turbidimetry detection reagent kit and application thereof.The reagent kit comprises a reagent R1 and a reagent R2.The reagent R1 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and interference elimination protein.The reagent R2 is prepared from a buffer solution, inorganic salt, a surfactant, a preservative, a stabilizer and a polystyrene latex particle mixture, and the polystyrene latex particle mixture is interlinked with a cystatin C antibody.The reagent kit based on latex-particle-enhanced turbidimetric immunoassay (PETIA) can be generally applied to analysis of various full-automatic biochemical analyzers and is short in assay time, good in specificity, high in precision and good in accuracy when used.

The invention provides a latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN. The latex-enhanced turbidimetric immunoassay kit comprises an adiponectin R1 reagent and an adiponectin R2 reagent, wherein the adiponectin R1 reagent comprises a buffer solution A, a protective agent A, a reaction enhancer and a preservative; the adiponectin R2 reagent comprises abuffer solution B, a protective agent B, a preservative and sensitized polystyrene latex particles coated with an anti-human adiponectin antibody; the anti-human adiponectin antibody in the sensitizedpolystyrene latex particle coated with the anti-human adiponectin antibody is connected with the sensitized polystyrene latex particle through a streptavidin-biotin system. The invention also provides a preparation method and an application method of the latex-enhanced turbidimetric immunoassay kit for quantitatively detecting adiponectin ADPN. The kit has the advantages that the kit is convenient, rapid, high in sensitivity and specificity and capable of quantitatively and accurately detecting ADPN; and the kit is strong in instrument compatibility and low in detection cost, and makes up theclinical requirements on ADPN turbidimetric products.

The invention provides a microalbuminuria (mAlb) detection kit, which is based on a latex enhanced turbidimetric immunoassay method, is a liquid double reagent, namely reagent R1 and reagent R2, wherein the reagent R1 is a reactant and the reagent R2 is a solution containing albumin immuno latex particles, and the detection kit is characterized by crosslinking by applying a chemical process, and covalently crosslinking a goat anti-human albumin antibody (also called goat anti-human mAlb antibody) with carboxylated polystyrene latex through water-soluble carbodiimide (EDC) and H-hydroxy succinimide (NHS), so as to form an mAlb antibody latex reagent. The reagent has the advantages of high sensitivity, strong specificity and simple preparation, and is worthy of further promotion.

Provided is a method for quantitatively measuring an antigen having diverse phenotypes with accuracy in immunoassay. The present invention is particularly intended to latex turbidimetric immunoassay using the antigen-antibody reaction of an antigen having phenotypes, wherein, in detection utilizing the immunoassay, the amount of an antibody against the antigen added to an assay system is adjusted and a basic amino acid is added to the assay system, thereby circumventing the variability of a measurement value attributable to phenotype variety and obtaining a measurement value having a high correlation with a measurement value of the antigen in a biological sample that is measured on a molecular basis.

There is a demand for improved turbidimetric immunoassays for human Cystatin C in biological samples, especially in human clinical samples of body fluids. The present invention provides a turbidimetric immunoassay method and reagent set enabling measurement of human Cystatin C by turbidimetric methods, resulting in a surprisingly stronger and faster turbidimetric signal than in the present state of the art. The increased and faster signal is accomplished by the use of new reagents and compositions, and enables shorter assay times and kinetic reading with a stronger signal, improving overall assay speed and quality. Improved robustness to lipid interference and improved linearity is achieved.

The invention discloses a latex-enhanced immune turbidimetric detection reagent for serum amyloid A (SAA‑Serum Amyloid A). The reagent is a single reagent, and the main component of the reagent is latex particles labeled with SAA antibody, and also includes buffer solution, surfactant, salt, stabilizer, suspending agent and preservative. The invention also discloses a method for using the reagent to detect the concentration of serum amyloid A (SAA) in blood samples by the principle of transmission or scattering turbidimetry. The invention adopts a single reagent, is simple to operate, does not need to be mixed, has high sensitivity, and has a wide linear range, and can directly measure various samples such as whole blood, serum, and plasma, and can be widely used in various transmission or scattering analyzers, including ordinary biochemical analyzers , specific protein analyzers, etc.

The invention relates to the field of in-vitro diagnosing reagents, and particularly relates to a method for improving the sensitivity of latex enhanced turbidimetric immunoassay. The method comprises the following steps: (1) selecting latex particles with large diameters to react with an antibody, and carrying out antibody coating; (2) washing and sealing the coated latex, dispersing the latex in a buffer solution to prepare a reagent 2 of a latex enhanced turbidimetric immunoassay kit; (3) preparing a reagent 1 of the latex enhanced turbidimetric immunoassay kit; (4) setting parameters on a full-automatic biochemical analyzer; (5) operating the biochemical analyzer to calibrate a sample with known concentration and detect an absorbance change value of the sample to be detected, and calculating the content of to-be-detected object in the sample according to the calibration curve. The method for improving the sensitivity of latex enhanced turbidimetric immunoassay is capable of greatly improving the analyzing sensitivity and precision of a reagent without increasing the detection cost, and has an excellent application prospect.

The invention discloses a latex enhanced turbidimetric immunoassay kit for detection of lipoprotein (a). The kit comprises reagents R1 and R2. The reagent R1 contains a Tris buffer solution, sodium chloride, polyethylene glycol and alkylphenol polyoxyethylene. The reagent R2 contains a Tris buffer solution, anti-human lipoprotein (a) antibody coated latex, EDTA, trehalose and alkylphenol polyoxyethylene. By proportionally mixing a sample to be detected and the reagent for a reaction, detecting absorbance change rate at dominant wavelength of 600 nm through a fully automatic biochemical analyzer and referring to absorbance change rate of a calibrator, concentration of lipoprotein (a) in the sample is calculated. The detection method of lipoprotein (a) in the invention is a latex enhanced turbidimetric immunoassay method. The kit of the method has advantages of high detection sensitivity, high accuracy, good stability, strong anti-interference performance and the like.

The invention belongs to the technical field of biology and relates to a preparation method of an adiponectin-latex enhanced turbidimetric immunoassay kit. A reagent R2 is composed of a reaction solution, a preservation solution and a latex microsphere antibody conjugate; the reaction solution is composed of a second buffer solution; and the latex microsphere antibody conjugate is composed of small latex microspheres, large latex microspheres, biotin, streptavidin, an adiponectin monoclonal antibody, an adiponectin polyclonal antibody and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride. After multiple research and development experiments, the large latex microspheres and the small latex microspheres are adopted; the large latex microspheres are coupled with the adiponectin monoclonal antibody, so that low-end sensitivity can be increased; the small latex microspheres are connected with the streptavidin and is mixed with the streptavidin proportionally; the adiponectin polyclonal antibody is connected with the biotin and is mixed with the biotin proportionally; and the two obtained mixtures are mixed proportionally and react with each other, and therefore, a linear rangecan be expanded; the two kinds of latex are mixed proportionally, so that repeatability and sensitivity can be improved, and a linear range can reach 1.00-32.00 mg / L.

An automatic analysis apparatus and an automatic analysis method that can perform both spectrophometric measurements for biochemical tests and the turbidimetric immunoassay with high precision by selectively exchanging a white light or at least one monochromatic light based on analysis conditions determined by a measure condition setting unit for respective measuring object. The automatic analysis apparatus includes an irradiating direction setting unit configured to irradiate the selected white light or at least one monochromatic light based on the analysis conditions onto a reaction cuvette along the same light axis and a light detection unit having a plurality of light receiving elements in order to detect the white light of particular determined wavelength lights and the selected monochromatic light.

The invention discloses a method of rapidly detecting concentration of heparin-combined proteins in blood. By means of a latex enhanced turbidimetric immunoassay method, an heparin-combined protein antibody is coated with latex particles to produce an antibody-latex particle composite; when the antibody-latex particle composite is specifically combined with the heparin-combined proteins in blood, the concentration of the heparin-combined proteins is measured by detecting the absorbance of a blood sample. The method is simple in operations, has high accuracy, allows accurate quantification and is suitable for detection of the heparin-combined proteins.

The invention relates to the technical field of troponin I detection, in particular to a troponin I detection reagent with high sensitivity through latex enhanced turbidimetric Immunoassay. The reagent comprises the following main components: a buffer solution, zinc chloride, Thesit, EMULGEN-A90, and a nitriloriacetic acid (NTA) preservative; a reagent R2 comprises the following main components: a buffer solution, triton-308, bovine serum albumin (BSA), a preservative, TnI antibody-coated latex particles and the like. The three surfactants, namely Thesit, EMULGEN-A90 and triton-308 are added, and the latex particles with appropriate particle sizes are selected, so that the reaction sensitivity is greatly improved, and the reagent is simple in product configuration, low in cost and very suitable for clinical expansion in large scale.

The invention provides a combined orientation agents optimized latex coupled antibody detection method of prealbumin (PA). The method is based on a latex enhanced turbidimetric immunoassay method, and is characterized in that combined orientation agents are utilized: an orientation agent 1 and an orientation agent 2. Firstly, the orientation agent 1 is combined with a PA antibody to form a PA antibody compound; the orientation agent 2 is then combined with the PA antibody compound; and finally, the compound is respectively coupled with carboxyl latex microspheres of two different particle sizes to form PA latex particles. By the method, the formed reagent has high sensitivity and good precision and linearity, is simple to prepare and is worthy of further promotion and application.

There is a demand for improved turbidimetric immunoassays for human Cystatin C in biological samples, especially in human clinical samples of body fluids. The present invention provides a turbidimetric immunoassay method and reagent set enabling measurement of human Cystatin C by turbidimetric methods, resulting in a surprisingly stronger and faster turbidimetric signal than in the present state of the art. The increased and faster signal is accomplished by the use of new reagents and compositions, and enables shorter assay times and kinetic reading with a stronger signal, improving overall assay speed and quality. Improved robustness to lipid interference and improved linearity is achieved.

The invention discloses a kit and a method for detecting the concentration of Kappa free light chains. The kit comprises a reaction buffer solution and a Kappa free light chain antibody solution, wherein the concentration of sodium chloride in the reaction buffer solution is 1250-2400 mmol / L. According to the kit, the problem of poor clinical specimen linearity in a turbidimetry assay of the Kappa free light chains is solved, reliable Kappa free light chain detection results as diagnostic references are provided for clinical doctors, the advantages of high speed and flux of a latex-enhanced turbidimetric immunoassay can be conveniently brought into full play, the detection time is shortened, and the clinical application and patient diagnosis are facilitated.

The invention discloses an alpha 1-microglobulin detection kit. The alpha 1-microglobulin detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution; the reagent R2 is a mixture of an alpha 1-microglobulin antibody-sensitization polystyrene latex particles and the buffer solution. The alpha 1-microglobulin detection kit disclosed by the invention has the advantages that 1, the detection is simple and quick, the sensitivity is high, the accuracy is good, the anti-jamming capacity is strong, and the production cost is low; 2, an alpha 1-microglobulin detection method adopted by the invention is a latex-enhanced turbidimetric immunoassay method, so that the alpha 1-microglobulin detection method enables the detection of alpha 1-microglobulin to be more economic, more convenient and quicker, is suitable for an automatic biochemical analyzer in most hospitals, and quick quantitative detection on emergency treatment can be specially realized.

Turbidimetric immunoassay of insoluble carrier particles that can inhibit the agglutination reaction of insoluble carrier particles such as latex by inhibiting the effect of plasma components that affect the measured value, stabilize the agglutination reaction, stabilize the absorbance of the reaction solution, and obtain accurate measurement results A reagent for measurement, a kit for insoluble carrier particle turbidimetric immunoassay, and a method for insoluble carrier particle turbidimetric immunoassay using the reagent or kit. In the presence or absence of a buffer containing a compound containing the following groups in the molecule, such as broad bean pyrimidine glucoside, tricoflavone, etc., the insoluble carrier particles are loaded with antibodies or antigens, and then, in the presence of the above buffer , the insoluble carrier particle suspension sensitive to the antibody or antigen is in contact with the test body, and an immunoagglutination reaction occurs, and the concentration produced is measured through the agglutination reaction of the insoluble carrier particles, and the antigen or antibody in the test body is quantified. (In the formula, R1, R2, and R3 can be the same or different, and represent a hydrogen atom, a hydroxyalkyl group, etc.)

The invention relates to a latex-enhanced turbidimetric immunoassay detection method, comprising the steps of: respectively adding a sample to be detected and a plurality of standards with different concentrations into different micro-wells of a well plate of a microplate reader; sequentially adding an R1 reagent and an R2 reagent into each of the micro-wells; and putting the well plate into the microplate reader for detection, wherein the feeding volume ratio of the sample to be detected or the standards, the R1 reagent and the R2 reagent is 1: 10 to 20: 20 to 30, the R1 reagent comprises a surfactant, and the R2 reagent comprises a latex microsphere coupled with an antibody. According to the latex-enhanced turbidimetric immunoassay detection method, an absorbance values of immune-particle complexes is detected by the microplate reader, and up to 96 specimens can be detected at one time, so that the detection speed is fast; the operation is convenient; the amount of required reagentsis small; the equipment cost is low; the popularity rate is high; a high energy detection is easy to realize; and the method is suitable for general medical institutions and underdeveloped areas.