Troponin I detection reagent with high sensitivity through latex enhanced turbidimetric Immunoassay

A troponin and detection reagent technology, applied in the field of latex-enhanced immune turbidimetric troponin I detection reagents, can solve the problems of low cost, cross-reaction, easy occurrence of zero value, etc., achieve protection activity and titer, improve Emulsifying effect, increasing sensitivity

Inactive Publication Date: 2016-06-01
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

The current detection methods include the determination of troponin I, which mainly includes immunological methods, and the detection methods include latex-enhanced immunoturbidimetric method, sandwich immunoradiometric method, sandwich enzyme-linked immunosorbent assay, chemiluminescence and electrochemiluminescence and other related methods, among which Ordinary sandwich immunoradiography and sandwich enzyme-linked immunoassay are relatively low in cost and relatively simple in operation, but if the antibody used in the test is a polyclonal antibody, it will cross-react and the analysis sensitivity is low
The two methods of chemiluminescence and electrochemiluminescence have good accuracy and high analytical sensitivity of the reaction, and can quickly and accurately detect the results, but these two methods require specific chemiluminescence instruments, and they are difficult for laboratory operators. The requirements are relatively high, so it cannot be widely used in clinical practice. Only some relatively large laboratories have the corresponding conditions. The latex-enhanced immunoturbidimetric method gradually developed and used in the later period is simple to operate and low in cost. It is better to operate in combination with automatic biochemical analysis instruments, which is convenient and quick, but this method also has the defect of low analytical sensitivity. During clinical use, it is easy to have zero or even negative values ​​for clinical samples with low concentrations. This brings great inconvenience to clinical users. Therefore, according to this problem, the author optimizes the detection of serum troponin I by latex-enhanced immune turbidimetry, and invents a latex-enhanced immune turbidimetric method with high analytical sensitivity. Serum Troponin I

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  • Troponin I detection reagent with high sensitivity through latex enhanced turbidimetric Immunoassay
  • Troponin I detection reagent with high sensitivity through latex enhanced turbidimetric Immunoassay
  • Troponin I detection reagent with high sensitivity through latex enhanced turbidimetric Immunoassay

Examples

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Effect test

Embodiment 1

[0039] A kind of existing common troponin I detection reagent, including reagent R1 and reagent R2, the composition of described reagent R1 and reagent R2 is as follows:

[0040] Reagent 1 (R1):

[0041] Glycine buffer ··················································· PH7.6

[0042] Sodium chloride ························································ 150mmol / L

[0043] Triton-100 ·················································· 1ml / L

[0044] Reagent 2 (R2):

[0045] Glycine buffer ·················································· PH7.6

[0046] TnI antibody-coated latex particles ··········································· Moderate

Embodiment 2

[0048] A troponin I detection reagent with high accuracy, comprising reagent R1 and reagent R2, the composition of the reagent R1 and the reagent R2 is as follows:

[0049] Reagent R1 contains

[0050] Reagent R1 contains

[0051] buffer ················································· 100mmol / L,

[0052] Zinc chloride ················································· 0.1mmol / L,

[0053] Thesit ················································· 1ml / L,

[0054] EMULGEN-A90 ············································ 1ml / L,

[0055] Aminotriacetic acid (NTA) ········································ 0.1g / L,

[0056] preservative ················································· 0.5ml / L;

[0057] 2) The components of reagent R2 are:

[0058] buffer ················································· 100mmol / L,

[0059] Triton-308 ············································· 1ml / L,

[0060] Bovine Serum Albumin (BSA) ······································ 1g / L,

[0...

Embodiment 3

[0064] Described in this example is a highly accurate troponin I detection reagent with increased content of important components, including reagent R1 and reagent R2, and the composition of the reagent R1 and reagent R2 is as follows:

[0065] 1) The composition of reagent R1

[0066] buffer ·················································· 100mmol / L,

[0067] Zinc chloride ·················································· 0.5mmol / L,

[0068] Thesit ·················································· 3ml / L,

[0069] EMULGEN-A90 ············································· 5ml / L,

[0070] Aminotriacetic acid (NTA) ········································· 1g / L,

[0071] preservative ·················································· 1ml / L;

[0072] 2) The components of reagent R2 are:

[0073] buffer ·················································· 100mmol / L,

[0074] Triton-308 ·············································· 5ml / L,

[0075] Bovine Serum Albu...

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Abstract

The invention relates to the technical field of troponin I detection, in particular to a troponin I detection reagent with high sensitivity through latex enhanced turbidimetric Immunoassay. The reagent comprises the following main components: a buffer solution, zinc chloride, Thesit, EMULGEN-A90, and a nitriloriacetic acid (NTA) preservative; a reagent R2 comprises the following main components: a buffer solution, triton-308, bovine serum albumin (BSA), a preservative, TnI antibody-coated latex particles and the like. The three surfactants, namely Thesit, EMULGEN-A90 and triton-308 are added, and the latex particles with appropriate particle sizes are selected, so that the reaction sensitivity is greatly improved, and the reagent is simple in product configuration, low in cost and very suitable for clinical expansion in large scale.

Description

technical field [0001] The invention relates to a high-sensitivity latex-enhanced immune turbidimetric troponin I detection reagent, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Troponin, consisting of T, C, and I trisubunits, together with tropomyosin, regulates actin-myosin interactions by regulating the activity of calcium ions on striated actin ATPase. when Myocardial damage back, cardiac troponin The complex is released into the blood, and after 4-6 hours, it begins to rise in the blood, and the elevated troponin I can remain in the blood for a long time of 6-10 days. Troponin I has a high degree of myocardial specificity and sensitivity, so troponin T has become the most ideal myocardial infarction landmark. [0003] Patients with various coronary artery diseases are bound to Cardiomyocytes Injury. The clinical manifestations of some patients may not fully meet the WHO diagnostic criteria for AMI (unstable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/32
Inventor 王珍李艳梅甘宜梧王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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