78results about How to "Effective chelation" patented technology

The invention relates to preparation and application of a novel high-polymer chelating agent which contains a heavy metal hazardous waste and is applied to stabilization treatment. The high-polymer chelating agent of the invention is an addition compound product of polyacrylamide and carbon disulfide, achieves good treatment effect on a solid or liquid waste and reaches the national waste landfill control standard. A synthesis process of the invention is simple, has low cost, can efficiently treat heavy metals in industrial or domestic garbage incineration wastes and is applied to a certain urban incineration fly ash curing enterprise.

The invention discloses a method for separating and culturing primary chicken hepatocytes. According to the method, EGTA (Ethylene Glycol Tetraacetic Acid) with low hepatotoxicity is added into a perfusate A to loosen the junction between hepatocytes, so that the dispersion degree of the to-be-separated hepatocytes is increased; double resistance is added into the perfusate A, a perfusate B and D-Hanks flushing fluid, so that the pollution is further prevented; and circulating perfusion digestion is adopted during digestion perfusion, so that the perfusion cost is greatly reduced. A serum-free cultural method of the chicken hepatocytes is established; compared with a traditional serum cultural method, the method disclosed by the invention has the same effect on the aspects of cell quantity, activity, function and the like, so that the problems caused by the existence of serum are solved and a foundation is laid for the substance metabolism study and biological product preparation on the basis of the chicken hepatocyte culture.

The invention relates to a preparation method of a core-shell structure tungsten/gadolinium oxide PVC calendered material for X-ray and gamma-ray protection. The preparation method comprises the following steps: firstly, preparing core-shell structure tungsten/gadolinium oxide powder; and preparing the core-shell structure tungsten/gadolinium oxide PVC calendered material from the powder. The core-shell structure tungsten/gadolinium oxide powder is uniformly smeared on a polyester imine film, the core-shell structure tungsten/ gadolinium oxide powder is melted into a core-shell structure tungsten/gadolinium oxide thin skin layer after high-temperature hot pressing, the core-shell structure tungsten/gadolinium oxide powder is added into PVC resin and uniformly mixed with the PVC resin, the mixture is sprayed on the surface of the core-shell structure tungsten/gadolinium oxide thin skin layer, after drying, the fabric is rolled and formed through a roller of a calendaring machine, and a lead-free light X-ray and gamma-ray protection material is prepared. The X-ray and gamma-ray protection material can play a synergistic protection role in radiation protection, eliminate a weak protection area and effectively absorb secondary radiation generated by radiation at the same time. The functional material has the characteristics of no lead and light weight, and has a good application prospect in the aspect of X-ray and gamma-ray radiation protection.

The invention relates to a three-element copolymerized hydrogel and a preparation method thereof. The preparation method is simple, and easy to implement; and the obtained three-element copolymerized hydrogel can effectively adsorb Cr3+, Cd2+, Cu2+ and Pb2+ ions. The preparation method for the three-element copolymerized hydrogel comprises the following step of: performing radiation polymerization on hydroxyethyl acrylate, n-ethylene dimethacrylate and 2-acrylamide-2-methylpropanesulfonic acid, wherein the molar ratio of the hydroxyethyl acrylate to the n-ethylene dimethacrylate to the 2-acrylamide-2-methylpropanesulfonic acid is (1-2):(0.1-0.6):(0.1-0.7); the n-ethylene dimethacrylate monomer is one of 2G, 4G, 9G and 14G; the radiation dosage of radiation polymerization is 1*103-1*106Gy;and the polymerization temperature is between -63 and -95DEG C. The invention has the advantages that: the three-element copolymerized hydrogel can effectively adsorb and chelate heavy metal ions; and the preparation method is simple, and easy to implement.

The invention discloses a primary rat or mouse gastric mucosal epithelial cell isolation and culture method. A perfusion method is innovatively introduced into the primary rat or mouse gastric mucosal epithelial cell isolation and culture method, and sufficient perfusion without dead corners is achieved. Prepared compound enzyme digestive juice avoids the problems of low cell viability and poor yield and is capable of digesting more evenly and thoroughly as compared with preheated digestion of traditional single collagenase. Within 12 hours of cell inoculation adherence, a cell culture medium with a pH value of 6-6.5 is adopted, cell adherence rate can be increased greatly and adherence effects are better. The primary rat or mouse gastric mucosal epithelial cell isolation and culture method has the advantages of large total cell number, high isolation degree, high living rate and low contamination probability by bacteria, fungi and other cells as well as capability of achieving subculture so as to provide a favorable cell culture scheme for related fundamental researches.

The invention relates to the technical field of troponin I detection, in particular to a troponin I detection reagent with high sensitivity through latex enhanced turbidimetric Immunoassay. The reagent comprises the following main components: a buffer solution, zinc chloride, Thesit, EMULGEN-A90, and a nitriloriacetic acid (NTA) preservative; a reagent R2 comprises the following main components: a buffer solution, triton-308, bovine serum albumin (BSA), a preservative, TnI antibody-coated latex particles and the like. The three surfactants, namely Thesit, EMULGEN-A90 and triton-308 are added, and the latex particles with appropriate particle sizes are selected, so that the reaction sensitivity is greatly improved, and the reagent is simple in product configuration, low in cost and very suitable for clinical expansion in large scale.

The invention discloses a biomass-based thermosensitive bionic hydrogel adsorption material and a preparation method and application thereof. The biomass-based thermosensitive bionic hydrogel adsorption material is prepared through the steps that taking a biomass material as a matrix, taking polyethyleneimine and N-isopropylacrylamide as functional reagents to modify the matrix, depositing polydopamine, and cross linking sodium alginate; and synthesizing the biomass-based thermosensitive bionic hydrogel adsorption material. The material has good performance when being applied to the field of adsorption of low-concentration anion heavy metal ions and organic dyes, the removal rate of the anion heavy metal Cr (VI with the initial concentration of 2000 ppb is larger than 99.99%, and the concentration of the removed heavy metal ions reaches the national drinking water standard. And the removal rate on low-concentration organic dye methylene blue (2000ppb) can also reach 95% or above.

The invention provides a mussel-shell micro-nano fruit and vegetable cleaning agent and a preparation method thereof. The components of the cleaning agent comprise a main material and an auxiliary material, wherein the main material is mussel shell powder, the auxiliary material is a marine organism active substance, and the cleaning agent comprises the following components in parts by weight: 90-100 parts of the mussel shell powder and 0-10 parts of the marine organism active substance. The preparation method comprises the following steps: cleaning mussel shells, carrying out impurity removing and drying, carrying out inert gas permeation and micro-puffing, carrying out uniform mixing with an activating agent, carrying out cooling, carrying out washing with distilled water, and carrying out drying and crushing to obtain mussel shell powder; and uniformly mixing the obtained mussel shell powder with the marine organism active substance to obtain the mussel shell micro-nano fruit and vegetable cleaning agent. Compared with a conventional fruit and vegetable cleaning agent, the fruit and vegetable cleaning agent provided by the invention mainly depends on adsorption of biomass materials, ion exchange, catalysis, alkaline effect and the like, bacteria can be effectively killed, and pesticide residues and heavy metals can be effectively removed. In addition, the marine active substance is added to be combined with a shell framework material, so that the heavy metals can be more effectively chelated and the residual pesticides can be buried more effectively.

The invention discloses an efficient heavy metal wastewater flocculating agent based on plant extracts and a preparation method thereof. The efficient heavy metal wastewater flocculating agent is prepared from the following raw materials in parts by weight: 2 to 5 parts of purslane extract, 1 to 4 parts of dandelion extract, 2 to 8 parts of dry lotus leaves, 5 to 10 parts of wheat straw, 10 to 15 parts of Selaginella tamariscina leaves, 1 to 4 parts of reed straw, 3 to 9 parts of hydroxyapatite, 2 to 6 parts of phosphate ore, 5 to 8 parts of oyster shell powder, 2 to 9 parts of trass volcanic ash, 7 to 10 parts of polylactic acid fiber, 5 to 12 parts of hydroxy acrylic resin, 5 to 9 parts of fatty alcohol-polyoxyethylene ether, 6 to 9 parts of Arabic gum, 3 to 9 parts of nano calcium sulfate, 5 to 10 parts of sodium diethylhexyl sulfosuccinate, 1 to 2 parts of sulfur, 1 to 4 parts of acraldehyde, 2 to 7 parts of magnesium silicate, 3 to 6 parts of gallotannic acid, 4 to 8 parts of a heat stabilizer, 2 to 5 parts of a coupling agent and 1 to 3 parts of a coagulant aid. The prepared wastewater flocculating agent is safe, stable and high in flocculation ability, metal ions can be effectively chelated, and the effect of wastewater treatment is reached. Simultaneously, the invention also discloses a corresponding preparation method.

The invention relates to a detection reagent for detecting lactic dehydrogenase isozyme 1 through a chemical inhibition method. The detection reagent comprises a reagent R1 and a reagent R2. The detection reagent is characterized in that the reagent R1 comprises a biological buffer solution in which a solute in the reagent R1 ranges from 50 mmol/L to 150 mmol/L in concentration, 1.0 mmol/L to 1.5 mmol/L of L-lithium lactate, 1.5 mmol/L to 2.5 mmol/L of sodium perchlorate, 1ml/L to 5ml/L of hydroxy ethidene diphosphonic acid, 0.1 g/L to 1g/L of octadecyl trimethyl ammonium bromide, 0.1 ml/L to 1 ml/L of polyoxyethylene alkyl ether, and 0.5 ml/L to 5 ml/L of preservative. The reagent R2 comprises a buffer solution in which a solute in the reagent R2 ranges from 50 mmol/L to 150 mmol/L in concentration, 8-12 mmol/L NAD+ and 0.5-5 ml/L of preservative. The lactic dehydrogenase isozyme 1 detection reagent has the advantages of being high in accuracy and cheap in price.

The invention provides a total cholesterol detection reagent with high accuracy and disturbance resisting capacity and relates to the technical field of total cholesterol detection. A biological buffer CAPS (3-(hexanaphthene amino)-1-propanesulfonic acid) is used in the reagent R, and therefore the buffering ability is ensured; a surfactant octadecy trimethyl ammonium bromide and a surfactant EMULGEN-707 are added into the reagent R, the emulsification effect of the reagent is improved well, and the accuracy and specificity of the reagent are improved; stabilizers such as trehalose and mannitol are added into the reagent R, and the stability of the reagent can be improved effectively; hydroxy ethidene diphosphonic acid is added into the reagent R, heavy metal ions can be effectively chelated, and the accuracy of the reagent can be improved; chemical-resistant succinoxidase and bilrubin oxidase are added into the reagent, disturbance of ascorbic acid and bilirubin can be effectively removed, and the reagent is simple in preparation, low in price and very suitable for large-area clinical popularization.

The invention provides an etching composition used in an etching process of a transition metal film such as an electrode of a TFT-LCD display. According to the etching composition disclosed by the invention, the metal ions generated when a transition metal film is etched can be effectively chelated, and the processing tension of the metal ions can be stably maintained for a long time, and the self-decomposition reaction of the composition can be effectively inhibited, so that the composition shows an excellent stability in which the etching process tension is not reduced, even when the etchingcomposition is used or stored for a long time.

The invention discloses a detection reagent for N-acetyl-beta-D-glucosaminidase with high analytical sensitivity. The reagent disclosed by the invention comprises a reagent R1 and a reagent R2; the reagent takes an artificially-synthesized product 5-(4-(3-methyl-styrene)-rhodanine-3-rhodanine-acetic acid amino-N-acetamino-beta-D-glucoside as a reaction substrate, so that the sensitivity of the reagent is improved; the reagent adopts two kinds of buffer solutions, i.e., an MES (2-(N-morpholine)ethyl sulfonic acid) buffer solution and an AMP (2-amino-2-methyl-1-propanol) buffer solution, so that the catalytic action of an NAG enzyme can be promoted while buffer capacity is guaranteed, a strongly-alkaline environment is effectively improved, and the efficiency of color development is guaranteed; ingredients are reasonably matched and used, so that the overall sensitivity, specificity and accuracy of the reagent are relatively high, and the further popularized use in market is facilitated.

A dirt dispersion agent for the industrial circulating cooling water treatment and a method for the production thereof are disclosed, the invention belongs to the chemical product technique field and is used for solving the problem of improving the scale inhibition and dispersion capabilities. The dirt dispersion agent has the technical proposal that the agent is copolymerized by three monomers of maleic acid, maleic acid monoester and acrylic acid, and the mole ratio of the three monomers of maleic acid, maleic acid monoester and acrylic acid is 2: 1-1.5: 0.5-1. The method of preparation of the invention comprises steps of esterification, copolymerization, etc.. The invention synthesizes a maleic acid-maleic acid monoester-acrylic acid terpolymer based on a maleic acid-acrylic acid copolymer of the prior art, the terpolymer has advantages of high effective constituent content and strong scale inhibition and dispersion capabilities as the dirt dispersion agent, and can effectively chelate Zn2+, and the terpolymer is in a dissolved state under rather high alkalinity, and can be used as the dirt dispersion agent of calcium carbonate and calcium sulphate in the industrial circulating cooling water treatment, and the scale inhibition rate can reach over 95 percent.

The invention belongs to the technical field of oil exploitation, and particularly relates to an oligomeric quaternary ammonium salt type heavy oil viscosity reducer and a preparation method thereof.The heavy oil viscosity reducer is an ethoxy quaternary ammonium salt type oligomer. A plurality of amphiphilic elements in the molecular structure of the oligomer have a relatively strong synergisticeffect and are arranged more closely on an oil-water interface, so the oligomer has excellent emulsifying capacity. On the other hand, molecules of the oligomer contains a plurality of EO elements which can effectively chelate heavy metal ions in heavy oil and destroy association of metal ions and asphaltene aromatic lamellas so as to destroy asphaltene aggregate structures. A single ethoxy quaternary ammonium salt type oligomeric surfactant provided by the invention can effectively reduce the viscosity of heavy oil with a high heavy metal content, and has a viscosity reduction rate of up to76-95%.

The invention discloses an oral care composition containing baking soda, application thereof and toothpaste containing baking soda. The oral care composition is composed of baking soda, sodium phytate and sodium fluoride according to the weight ratio of 1:(0.04-0.1):(0.01-0.024). The oral care composition is high in stability, can neutralize dental plaque acid and whiten teeth, and can be matched with other carrier components to prepare various oral care products. The toothpaste containing baking soda has high stability and can deeply clean the oral cavity, whiten and strengthen teeth and nourish gingivae.

The invention discloses selenium-enriched modified milk powder containing probiotics and a preparation method of the selenium-enriched modified milk powder. The selenium-enriched modified milk powdercontaining the probiotics comprises the following components in percentage by weight of 50%-75% of a milk source, 15%-25% of a milk protein raw material, 5%-10% of fructo-oligosaccharide, 4-8% of galactooligosaccharide, 0.2%-0.5% of chitosan oligosaccharide, 0.2%-0.4% of oyster peptide powder, 0.000018%-0.00003% of L-selenium methylselenocysteine and 0.2%-0.4% of bifidobacterium, wherein the sum of the weight percentages of the components is 100%. The selenium-enriched modified milk powder containing the probiotics is rich and comprehensive in nutrition, good in absorbability and beneficial tointestinal health.

The invention relates to a preparation method of a copolymer, which comprises the following steps: evenly mixing N-vinylformamide monomer, 2-hydroxyethyl acrylate monomer and water, wherein the volume ratio of N-vinylformamide monomer to 2-hydroxyethyl acrylate monomer is 1:1-1:9; and charging a protective gas, and carrying out high-energy ray irradiation polymerization at -63--95 DEG C. The invention also relates to a copolymer prepared by the preparation method; and the copolymer has a good pore structure, contains a functional group capable of adsorbing heavy metal ions, has high adsorption capacity for Cr<3+>, Cd<2+>, Cu<2+> and Co<2+>, and has a certain mechanical strength and long service life. In addition, the preparation method provided by the invention does not need to add any initiator or crosslinking agent, thereby ensuring the purity of the copolymer; and the preparation method is simple and easy to operate, and has the advantage of lower production cost.

The invention provides a preparation method of core-shell structure tungsten/gadolinium oxide PP non-woven fabric for X-ray and gamma-ray protection. The preparation method comprises the following steps: firstly, preparing core-shell structure tungsten/gadolinium oxide powder; preparing a W (at) Gd2O3/PP blended melt according to the powder; then preparing a W-coated Gd2O3/PP composite fiber according to the blended melt; and finally, preparing the W-coated Gd2O3/PP composite non-woven material according to the composite fibers. The core-shell structure tungsten/gadolinium oxide PP non-woven fabric prepared according to the method can play a synergistic protection role in radiation protection, and secondary radiation generated by radiation can be effectively absorbed while weak protection areas are eliminated. Secondly, the prepared functional non-woven fabric has the characteristics of no lead and light weight, and has a good application prospect in the aspect of X-ray and gamma-ray radiation protection.

The invention discloses a detection reagent for N-acetyl-beta-D-glucosidase. The detection reagent comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises 10-100mmol/L of citric acidbuffer solution, 0.1-0.3g/L of substrate, 1KU/L-5KU/L of ascorbic acid oxidase, 0.01-0.03g/L of magnesium chloride, 1-10g/L of stabilizer, and 2g/L-5g/L of preservative, the reagent R2 comprises 10-100mmol/L of sodium carbonate buffer solution, 3mL/L-5mL/L of triton X-100, and 2g/L-5g/L of preservative, the citric acid buffer solution is at 25 DEG C and the pH thereof is 4-5, the substrate is 5-[4-(3-methoxy-benzophene-rhodanine)-3-ammonium acetate-N-acetylamino-beta-D-glucoside, the stabilizer is one of EDTA, EDTA-2Na, glycol, and diglycolamidic acid, and the preservative is one of NaN3, methylparaben, and 4-chloro-3,5-dimethylphenol. A synthesized substrate 5-(4-(3-methyl-styrene)-rhodane-3-acetic acid amino-N-acetamino-beta-D-glucoside is used as a reaction substrate, the substrate isrelatively low-cost, and a reactant has high color development performance, so that the reagent is more sensitive.

The invention discloses a method for extracting and obtaining a bromelain from pineapple peel residues, which comprises the following steps of: cleaning pineapple peel residues, putting the pineapplepeel residues into a crushing and filtering device for multi-stage crushing, adding 0.05-0.1 % of ascorbic acid and 0.5-1 Kg of distilled water, and finally obtaining filtrate through extrusion and filtration; adding 0.03-0.05 % of calcium propionate into the filtrate for corrosion prevention, stirring and centrifuging the filtrate at a high speed for 10-15 minutes at a stirring speed of 4000-5000r/min, taking supernatant, adding pre-dissolved tannin solution into the supernatant, stirring while leading the final concentration of the tannin to be 0.1-0.2 %, and allowing the solution to standafter adding the tannin. The enzyme activity of the bromelain prepared from the pineapple peel residues is good, the purity is high, and the utilization rate of pineapple peel residues is improved.

The invention relates to a blood ammonia (AMM) detection reagent and in particular relates to a high-stability single-reagent liquid-state blood ammonia (AMM) detection reagent. The reagent is mainly prepared from main substances including a buffering solution, LDH (Lactate Dehydrogenase), a heavy metal ion chelating agent, an ionic balance agent, a preservative, alpha-KG, NADH.NA2, a surfactant, a protecting agent and the like. According to the reagent, a single reagent of glutamate dehydrogenase two-point method and kinetic method is established; the utilization operation is effectively simplified and the stability of effective components of a product is guaranteed; the metal chelating agent is properly selected so that the inhibition, caused by the heavy metal ions, on the activity of an enzyme in the reagent is effectively removed; interference on the enzyme is not caused; the protecting agent is properly added so that the stability of the enzyme and the stability of key substrates in the reagent are effectively protected; the substances are added so that the stability of a single-reagent product provided by the invention is effectively guaranteed and the reagent is greatly convenient for utilization and popularization clinically.

The invention discloses an ultralow-emission stabilizer for a boiler water system and a preparation method of same. The ultra-low emission stabilizer comprises a scale inhibitor, a corrosion inhibitor, a regulator and a solvent, which are mixed together, wherein the scale inhibitor includes at least one of EDTA disodium salt, trisodium nitrilotriacetate and hydroxyphosphoryl acetic acid; the corrosion inhibitor includes at least one of sodium molybdate, azodicarbonamide and LAN-826; the regulator is at least one of sodium hydroxide, potassium hydroxide and sodium carbonate; the solvent is desalted water. The ultra-low emission stabilizer is added to a steam pocket of a boiler.