65 results about "Chlorohydrocarbon" patented technology

The present invention discloses a sulfurized nano zero-valent iron material, its preparation method and application, including: a core-shell structure sulfurized nano zero-valent iron material and a preparation method, the inner core of the sulfurized nano zero-valent iron is zero-valent iron, and the outer shell is Fe9S 10 , calculated by the mass of the sulfurized nano zero-valent iron, the mass percentage of zero-valent iron is 70-80%, and the mass percentage of the iron sulfide is 20-30%; the preparation method includes: placing iron powder in an H2S gas atmosphere for calcination, after the calcination is completed, cooling to room temperature to obtain the sulfurized nano zero-valent iron material. This material can be used for in-situ remediation of chlorinated hydrocarbon organic pollutants in groundwater. Compared with the prior art, the prepared sulfurized nano zero-valent iron is less likely to be oxidized and can overcome the problem of rapid passivation of nano zero-valent iron during the remediation process.

The application discloses a kind of heteropoly acid assisted ball milling modified micron zero-valent iron particles, preparation method and application.The micron zero-valent iron particles are mainly prepared by mixing micron zero-valent iron, heteropoly acid and relatively cheap noble metal compound, and the specific preparation is that micron zero-valent iron, heteropoly acid and noble metal compound are placed in a ball mill jar and a specific atmosphere to carry out ball milling modification to obtain heteropoly acid modified ultra-low ruthenium loaded micron zero-valent iron particles.The particles provided by the application have extremely strong reaction performance on chlorinated hydrocarbons, and the performance does not attenuate for more than 30 cycles, and can achieve complete dechlorination on low concentration and high concentration chlorinated hydrocarbons, which has great water treatment application value.

The invention provides a biochar-zero-valent iron enhanced bentonite-based vertical barrier material for combined polluted underground water, which comprises sodium modified calcium bentonite and sandy soil, and also comprises biochar and slow-release zero-valent iron particles, and the slow-release zero-valent iron particles are formed by mixing and stirring micron-sized zero-valent iron and sodium alginate. Related preparation methods and uses are also provided. The anti-seepage performance of the biochar-zero-valent iron enhanced bentonite-based vertical barrier material for combined polluted underground water meets the anti-seepage requirement that the permeability coefficient is lower than 10 <-9 > cm / s under the stress of heavy metal, chlorinated hydrocarbon and micro-plastic combined polluted underground water, heavy metal can be effectively reduced and precipitated, chlorinated hydrocarbon is dechlorinated, and micro-plastic is adsorbed; therefore, the concentration of composite pollutants in underground water is reduced, and the long-acting performance of the vertical barrier material is improved.

The present invention relates to the technical field of environmental pollution remediation, and specifically discloses a bioremediation method for degrading chlorinated hydrocarbons using microorganisms in collaboration with colloidal coconut shell biochar, and its application. The method comprises: preparing a combined bacterial agent, preparing colloidal coconut shell biochar powder, constructing a collaborative degradation system and designing a reaction system, and on-site in-situ remediation application. The method effectively overcomes the problems of insufficient microbial activity, unstable electron donor supply, and saturation of adsorbent materials in traditional bioremediation. The present invention can always maintain the remediation process in an optimal state, effectively improving the engineering practicality and reliability of on-site remediation. The method is simple to operate, has low energy consumption, and is cost-effective. It also has high environmental adaptability and long-term remediation effects, conforming to the concept of green remediation. The technical solution of the present invention is not only suitable for ex-situ remediation verification, but can also be extended to actual engineering applications in complex contaminated sites, with broad market prospects and promotional value.

The invention discloses a preparation method of a granular iron-based biochar material. The preparation method comprises the following steps: a, performing acid pickling on a waste biomass raw material, and performing drying and crushing to obtain biomass raw material powder; b, immersing the biomass raw material powder obtained in the step a into an iron salt solution, filtering and drying to obtain an iron-loaded biomass raw material; c, mixing and granulating the iron-loaded biomass raw material obtained in the step b, an adhesive, an expanding agent and water, and drying to obtain a material precursor; and d, pyrolyzing the material precursor obtained in the step c in an inert atmosphere to prepare the granular iron-based biochar material. According to the granular iron-based biochar material prepared by the method, aggregation of metal nanoparticles can be effectively reduced, effective load of zero-valent iron is improved, the removal rate of chlorinated hydrocarbon is improved, and meanwhile, the granular material solves the problems that a powder material is easy to corrode and passivate and gaps are blocked; the method can be used for repairing the chlorohydrocarbon polluted soil-underground water.

The present invention belongs to the field of remediation of groundwater contaminated by organic compounds, and discloses an enhanced microbial remediation device and a remediation method for groundwater contaminated by organic compounds, which can achieve efficient and synergistic remediation of groundwater contaminated by a mixture of chlorinated hydrocarbons and benzene series compounds. The enhanced microbial remediation device includes a hollow container, a base, a first syringe, a first injection pump, a second syringe, and a second injection pump; the bottom end of the container is connected to the outlet of the first syringe through a pipeline, the first syringe is connected to the first injection pump, a medium layer, a microbial sphere layer of immobilized bacteria for chlorinated hydrocarbon degradation, and a microbial sphere layer of immobilized bacteria for benzene series compound degradation are provided in the container, and the microbial sphere layer of immobilized bacteria for chlorinated hydrocarbon degradation is located below the microbial sphere layer of immobilized bacteria for benzene series compound degradation; an injection port is provided on the side wall of the container, the injection port is connected to the outlet of the second syringe through a pipeline, and the second injection pump is connected to the second syringe.

The invention discloses a filler for repairing chlorinated hydrocarbon pollution and a method for repairing chlorinated hydrocarbon polluted underground water by using the filler. The filler is prepared from the following components in percentage by mass: 65-85% of coarse sand, 1-10% of magnetite, 1-5% of groundwater polluted by chlorinated hydrocarbon, 1-5% of wheat bran, 1-5% of coarse gypsum, 1-5% of ferrous sulfate and 1-5% of limestone. The raw materials of the filler are industrial finished products, are low in price and are suitable for actual site repair. The method for repairing chlorohydrocarbon polluted underground water through biogeochemical conversion by using the filler does not need high temperature and high pressure, and the reaction condition is mild. The used filler and emulsified vegetable oil have the advantages of high safety and environmental friendliness, and do not cause secondary pollution to the underground environment. The generation and accumulation of incomplete dechlorination intermediate products can be obviously reduced, so that the chlorinated hydrocarbon can be completely dechlorinated.

The invention belongs to the technical field of sewage treatment, and particularly relates to a sulfur conversion driven chlorohydrocarbon polluted underground water treatment method. According to the method, the multifunctional biochar is prepared through high-temperature ammonia gas modification, PS can be activated to oxidize and remove chlorinated hydrocarbon, then sulfide reduction is catalyzed to remove residual chlorinated hydrocarbon, a porous structure of BC provides pollutant adsorption and fixation functions, and after MFBC, PS and sulfate reducing bacteria are sequentially added into a chlorinated hydrocarbon pollution medium, the pollution medium is subjected to high-temperature ammonia gas modification to remove the residual chlorinated hydrocarbon. The sulfur-containing species undergo a cyclic conversion process from PS-sulfate ion-sulfide-elemental sulfur-sulfate ion. In the conversion process, various oxidation and reduction active substances can be used for effective detoxification of chlorinated hydrocarbon, and MFBC and sulfate reducing bacteria play a key role in mediating sulfide conversion. According to the method, the chlorohydrocarbon in the high-permeability medium can be rapidly removed firstly, and then the chlorohydrocarbon slowly released by the low-permeability medium can be slowly removed at low cost. And the method is low-carbon and environment-friendly, has no secondary pollution such as heavy metals and has obvious application advantages.

The invention relates to the technical field of molecular biology and environmental biology, and discloses a PCR (Polymerase Chain Reaction) chip and a kit for detecting the expression quantity of functional genes related to microbial degradation of chlorinated hydrocarbon and application of the PCR chip and the kit. The PCR chip comprises a primer pair and a primer pair, the primer pair is used for specifically amplifying dehalogenation functional genes bvcA, cfrA, mbrA, pceA, pteA, tceA and vcrA respectively, the primer pair is used for specifically amplifying reference genes, and nucleotide sequences of the primer pair for specifically amplifying the dehalogenation functional genes are shown as SEQ ID NO. 1 to SEQ ID NO. 14. The PCR chip provided by the invention can specifically amplify seven functional genes related to the degradation of the chlorinated hydrocarbon microorganisms, is good in specificity, high in accuracy and good in repeatability, and can accurately detect the expression condition of the seven dehalogenation functional genes related to the degradation of the chlorinated hydrocarbon microorganisms through a qPCR technology, so that the existence condition of chlorinated hydrocarbon degrading bacteria can be reflected; the method has reliability in the aspects of chlorohydrocarbon polluted site biodegradability evaluation, degradation process research, repair effect verification and the like.

The application discloses a nano zero-valent iron modified hemicellulose biochar composite material and a preparation method and application thereof. The preparation method comprises the following steps: mixing xylan raw materials and iron oxide, performing ball milling treatment, and then performing a carbothermic reduction process in a microwave pyrolysis device under a nitrogen protection atmosphere and a constant temperature condition, so as to obtain the nano zero-valent iron modified hemicellulose biochar material after cooling. The composite material is prepared by using the above preparation method. The composite material is applied to purification treatment of polluted wastewater containing chlorinated hydrocarbons. The composite material has low raw material cost, simple preparation method, can form stable structure pores, has good adsorption performance and catalytic activity, realizes molecular dispersion of zero-valent iron in a hemicellulose biochar matrix, and improves adsorption and reduction capacity of the composite material on chlorinated hydrocarbon pollutants.

The present application provides a preparation method of an aromatic hydrocarbon oligomer, the preparation method including: subjecting an aromatic hydrocarbon compound to an oligomerization reaction under the catalysis of a chloroaluminate ionic liquid to obtain the aromatic hydrocarbon oligomer, where the oligomerization reaction is carried out in a solvent system, and the solvent is selected from chlorinated hydrocarbon solvents, and the aromatic hydrocarbon compound is selected from fused-ring aromatic hydrocarbons with 2 to 4 rings. The preparation method of the present application can efficiently prepare aromatic hydrocarbon oligomers with simple composition and narrow molecular weight distribution under mild conditions, thereby providing a high-quality precursor for the preparation of high-quality mesophase pitch.

The invention discloses hydrocarbon resin with low dielectric constant and high heat resistance as well as a preparation method and application thereof, and the preparation method of the hydrocarbon resin with low dielectric constant and high heat resistance comprises the following steps: S1, in an organic solvent, adding a polycyclic aromatic hydrocarbon compound and a chlorinated hydrocarbon compound for reaction; s2, adjusting the pH value of a reaction system to be neutral, and removing a water phase; s3, part of the organic solvent is removed through distillation till the set solid content is achieved, and a low-dielectric high-heat-resistance hydrocarbon resin solution is obtained after cooling. The low-dielectric high-heat-resistance hydrocarbon resin solves the problems that traditional hydrocarbon resin is insufficient in rigidity, low in strength, poor in heat resistance and low in glass transition temperature after being cured.

The invention relates to a biochar-loaded nano zero-valent iron repair material as well as a preparation method and application thereof. The biochar loaded nano zero-valent iron repair material is prepared from a persulfate-biochar carrier and metal sulfide modified composite acid stabilized nano zero-valent iron sol, the composite acid is composed of gallic acid and humic acid / fulvic acid; the preparation method comprises the following steps: S1, preparing a persulfate-biochar carrier; and S2, preparing the biochar loaded nano zero-valent iron repair material. The method is applied to groundwater chlorohydrocarbon pollution remediation. According to the method, in the reductive dechlorination process of the nano zero-valent reduced iron, released Fe < 2 + > can strengthen persulfate, Fe < 2 + > is regenerated by utilizing the reduction effect of gallic acid on reacted Fe < 3 + >, and catalytic circulation of iron is maintained; humic acid / fulvic acid is used for efficiently driving the degradation process; and metal sulfide is utilized to catalyze reduction of organic chlorine chemicals, so that the reductive dechlorination performance of the nano-iron material is improved, and the repairing effect of chlorinated hydrocarbon is optimized.

The invention belongs to the technical field of inorganic non-metallic materials, and discloses a 3D printing high-thermal-conductivity precursor converted silicon carbide ceramic and a preparation method and application thereof.The preparation method comprises the following steps that silicon carbide powder, modified B4C nano powder and polycarbosilane powder are added into excessive aromatic hydrocarbon or / and chlorinated hydrocarbon organic solvents according to the proportion, stirring is conducted, and a mixture is obtained; uniformly stirring in an inert atmosphere, putting into a planetary ball mill, carrying out ball milling, setting the rotating speed time, and uniformly mixing the raw materials to obtain slurry; after the solid content of the slurry is adjusted to a set value, a ceramic green body is prepared through 3D printing, the green body is dried and sintered, and a precursor converted silicon carbide ceramic part is prepared; the preparation method comprises the following steps: adding silane coupling agent modified nano Si powder, beta-SiC powder and phenolic resin into ethanol according to a ratio, and uniformly mixing to obtain an impregnation liquid; and carrying out vacuum impregnation on the precursor-converted silicon carbide ceramic piece in the impregnation liquid, and after impregnation is completed, carrying out curing and sintering so as to obtain the silicon carbide ceramic with high mechanical strength and good heat-conducting property.

The invention relates to a compound microbial agent and application thereof in repairing or controlling chlorinated hydrocarbon contaminated sites, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise Gordonia, pseudomonas, pseudoxanthomonas and geomonas in a viable count ratio of 100: (25-600): (10-900): (10-500). The method effectively solves the problem that the chlorohydrocarbon contaminated site is difficult to repair, overcomes the problems of low mass transfer efficiency, limited microbial activity and the like of the traditional repair technology, improves the repair and risk management and control efficiency of the chlorohydrocarbon contaminated site, and reduces the construction cost. The method is especially suitable for green and efficient remediation of soil and underground water in silt soil sites, pollution source reduction and risk management and control, has the advantages of low cost, convenience in operation and the like, and has important application prospects.

The present application relates to the technical field of biological stimulation remediation of chlorinated organic pollutants in aquifer, and discloses a chlorinated hydrocarbon remediation material, a preparation method thereof and a method for in-situ remediation of chlorinated hydrocarbon in aquifer. The chlorinated hydrocarbon remediation material contains organic matter A, organic matter B and organic matter C, the organic matter A wraps the organic matter B and the organic matter C; the molecular weight of the organic matter A is 1000-30000, the molecular weight of the organic matter B is 200-2000, and the molecular weight of the organic matter C is 100-400. The method for preparing the chlorinated hydrocarbon remediation material comprises the following steps: S1, under the protection of inert atmosphere, first mixing the organic matter A with an organic solvent, and then second mixing the obtained first mixed solution with the organic matter B and the organic matter C; S2, drying the second mixed solution obtained in step S1. The chlorinated hydrocarbon remediation material can maintain solid-phase reduction dechlorination components for a long period of operation, and the effective slow-release period thereof can be significantly increased.

The present invention discloses a method for reducing dechlorinated chlorohydrocarbons by coupling a compound surfactant with a sulfide micron zero-valent iron system, and belongs to the technical field of in-situ chemical remediation of water bodies. The present invention uses a compound surfactant HTAB and Tween80 coupled with a sulfide micron zero-valent iron material as a reducing agent to reduce chlorinated hydrocarbons in water bodies. Under the effective regulation of the compound surfactant, the sulfide micron zero-valent iron is promoted to efficiently reduce carbon tetrachloride. The compound surfactant coupled with the sulfide micron zero-valent iron is superior in reusability to the HTAB coupled with the sulfide micron zero-valent iron system, and has a higher removal rate for chlorinated hydrocarbons (carbon tetrachloride). The surfactant compound system reduces the amount and cost of the cationic surfactant HTAB without causing secondary pollution to the environment. In addition, the method provided by the present invention has a simple preparation process, a fast reaction rate, a strong ability to remove a variety of chlorinated hydrocarbon pollutants, a wide applicable pH range, and is more suitable for practical applications.

The invention discloses an improved groundwater remediation agent and a preparation method thereof. The groundwater remediation agent is granular, and each particle is of a multi-layer structure; wherein the outer layer of the particle is coated nano zero-valent iron, the middle layer of the particle is an isolating layer, and the inner layer of the particle is crystalline or amorphous sodium percarbonate. The improved remediation agent disclosed by the invention has a slow release property, the chlorohydrocarbon is firstly dechlorinated by the zero-valent iron aiming at underground water polluted by the chlorohydrocarbon, and the hydrogen peroxide released by the sodium percarbonate completes oxidation elimination on the chlorohydrocarbon and an intermediate product under the catalysis of ferrous iron formed by dechlorination along with the proceeding of the process.

The invention belongs to the technical field of soil remediation, and particularly relates to a remediation material for degrading polluted soil chlorinated hydrocarbon and a preparation method of the remediation material. The repair material for degrading the chlorinated hydrocarbon in the polluted soil is prepared from the following raw materials: humic acid, rhamnolipid, a biological agent and diatomite. The final repairing material utilizes mutual complementation and mutual synergism of humic acid, rhamnolipid, the biological agent and diatomite, has a remarkable effect in the aspect of degrading trichloroethylene, cis-1, 2-dichloroethylene, trichloroethane and 1, 2-dichloroethane, can be used for repairing chlorohydrocarbon polluted soil, and achieves a better degradation effect.

The invention discloses a method for rapidly stripping an aluminum-plastic film composite material. The method comprises the following steps: S1, confirming a recovery target; s2, selecting a stripping method; s3, the aluminum plastic film is soaked in the mixed solution and then subjected to spin-drying, water washing, water flotation, re-spin-drying and hot air drying, and an aluminum foil and a whole plastic film are obtained; s4, checking and cleaning; and S5, waste treatment. According to the method, toxic substances and volatile chlorinated hydrocarbon are not used, and the chlorinated hydrocarbon is high in recovery cost and low in recovery rate, so that the cost is low; and the solution soaking and water flotation methods combine the advantages of high efficiency, environmental protection, simplicity and convenience in operation, high resource recovery rate and the like.

The present application relates to the technical field of remediation of aquifer pollution, and particularly relates to a chlorinated hydrocarbon pollution remediation composition, application thereof and an in-situ remediation method for aquifer chlorinated hydrocarbon pollution. The composition comprises component A and component B; component A comprises a carbon source, a surfactant and water; the carbon source is selected from fatty acids and / or fatty acid esters; the total weight of component A is 100 parts, the content of the carbon source is 10-50 parts, the content of the surfactant is 2-15 parts, and the content of water is 35-88 parts; component B comprises a zinc salt. In field application, component A is first injected into the remediation site, and then component B is injected; through the injection of component B, component A can be transformed from the state of oil-in-water stable and uniform emulsion to the state of oil phase, and can be fixed in situ for a long period, thereby providing a carbon source for the biological stimulation of microorganisms to degrade chlorinated hydrocarbons.

The invention discloses a simulation test system and a simulation test method for a process of phytoremediation of chlorinated hydrocarbon pollutants, the simulation test system comprises a plant experiment box, a water supply system, a monitoring assembly, a liquid recovery bottle and an imaging system, the water supply system, the plant experiment box and the liquid recovery bottle are sequentially connected in series through a hose; the imaging system consists of a lighting lamp box, a camera and a computer; the plant experiment box is used as a main body, so that the influence of plants on the distribution of pollutants in a porous medium in different growth environments can be flexibly simulated, and the change of physicochemical property parameters around a root system can be monitored in real time; by tracking the change of the distribution condition of pollutants in a two-dimensional system, the pollutant removal process of phytoremediation is studied, and the removal effect and action mechanism of different plant growth regulation and control technologies, solubilization strengthening technologies and the like on organic pollutants of plants in a simulated porous medium are verified.

The invention belongs to the technical field of paint removers, and particularly relates to a star-structure silane sealing agent, a water-based paint remover and a preparation method and application of the star-structure silane sealing agent and the water-based paint remover. According to the invention, a star-shaped structure silane sealing agent with annular polysiloxane as a core and polyol as a side arm is designed and synthesized, the star-shaped structure silane sealing agent is added into a water-based paint remover, the annular polysiloxane shields and blocks volatilization of polar components in the paint remover, and the polyol side arm is combined with the polar components through hydrogen bond acting force, so that the water-based paint remover is formed. The volatilization failure of alcohol, ester or ketone solvents in the high-temperature paint removal process is avoided, the paint can be removed at a higher temperature, and the paint removal time is shortened. The polyol on the side arm of the sealing agent also has the effect of chelating inorganic ions, so that corrosion caused by deposition of metal ions in the aluminum alloy and influence of precipitation of calcium and magnesium ions in water on the emulsification effect of the oil phase component are avoided. In addition, the water-based paint remover does not contain organic solvents such as chlorinated hydrocarbon, phenols, acetone and the like, and has no influence on the health of operators and the environment.

The application provides a method for degrading chlorohydrocarbon in water by using illite reinforced ball-milling sulfidation micro zero-valent iron, which comprises the following steps: first, a buffer solution with pH=7 is prepared by using 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, and an oxygen-free aeration treatment is performed; then, illite and S-mZVI bm are mixed to prepare a particle mixture, and the particle mixture is sealed and stored in the buffer solution with pH=7; finally, chlorohydrocarbon pollutants are added into the particle mixture of illite and S-mZVI bm , and a sealed oscillation reaction is performed, so that the concentration of the chlorohydrocarbon in the reaction system is determined. The application directly reinforces the activity of S-mZVI bm material by using illite, which is a clay mineral widely distributed in nature, so that the defects of S-mZVI bm , such as large particle size, slow degradation rate and decreased activity in the later reaction stage, are effectively solved, and the long-term reaction activity of the particles is ensured. The raw materials are green and environmentally friendly, the process is simple, and the materials are easy to obtain.

The invention relates to the technical field of environmental remediation, and particularly discloses a method for remedying chlorinated hydrocarbon pollution, a chlorinated hydrocarbon pollution remediation agent and application, and the method comprises the steps that an injection point is arranged on a chlorinated hydrocarbon pollution site; preparing a sodium persulfate solution with the mass concentration of 7.5%-15%, and preparing a mixed solution of ferrous sulfate with the mass concentration of 1.6%-3.2% and sodium pyrophosphate with the mass concentration of 1.25%-2.5%, namely an iron pyrophosphate mixed solution; drilling is carried out according to the set injection points, drilling is carried out to the pollution depth of the chlorinated hydrocarbon pollution site, and a sodium persulfate solution is sprayed from deep to shallow; spraying a ferric pyrophosphate mixed solution in the same manner, after 7-14 days of chemical oxidation remediation, spraying 5-10 L of emulsified vegetable oil, and naturally remediating for 30-35 days. According to the method, the concentration of chlorohydrocarbon pollutants in chlorohydrocarbon polluted site soil and underground water is remarkably reduced.

The invention relates to a continuous production method of carbon tetrachloride, which comprises the following steps: mixing and vaporizing perchlorinated hydrocarbon and chlorine, and carrying out chlorination reaction at the temperature of 200-500 DEG C under the pressure of 0.6-1 MPa to obtain a crude product; performing gas-liquid separation on the crude product, returning the gas to the mixed vaporization step for recycling, and rectifying the liquid to obtain a carbon tetrachloride product; and returning other products obtained by rectification to the mixed vaporization step for recycling. In the production process, the raw materials are perchloro and chlorine, no hydrogen element participates in the production process, the required temperature and pressure conditions are relatively low, the reaction conforms to the concept of green chemistry, and the utilization rate of chlorine atoms in the whole process is 95% or above.

The invention relates to a compound surfactant, a repairing material and a method for repairing chlorinated hydrocarbon pollutants. The compound surfactant is simple in component, good in solubilizing effect, green and low in toxicity, can effectively reduce the surface tension of an aqueous solution when being dissolved in water, and has relatively high low-temperature water solubility. The repairing material has a good solubilizing effect on hydrophobic chlorinated hydrocarbon, and when the repairing material is used for repairing the chlorinated hydrocarbon pollutants, the problems that in the prior art, a surfactant is poor in solubilizing effect on the chlorinated hydrocarbon pollutants in the underground water environment, cannot be suitable for the underground low-temperature application environment, has secondary pollution and the like are effectively solved.

The invention belongs to the technical field of environmental functional materials, and particularly relates to a cobalt-nitrogen modified zero-valent iron ternary nitride material, preparation thereof and application of the cobalt-nitrogen modified zero-valent iron ternary nitride material in removal of chlorinated hydrocarbon. The invention provides a cobalt nitrogen modified zero-valent iron ternary nitride material which can be used for removing chlorinated hydrocarbon in a water body. Zero-valent iron serves as a matrix, cobalt powder, sodium amide and zero-valent iron powder are mixed through a mechanochemical method, ball milling is carried out under the inert atmosphere, and the cobalt-nitrogen synergistic modified ternary nitride structure is formed. The cobalt powder and the sodium amide are doped into the surface and the interior of the zero-valent iron, so that the zero-valent iron generates lattice strain, the chlorohydrocarbon dechlorination capacity is improved, the preparation process is simple, low in cost, free of pollution and suitable for large-scale application, and the problems of low degradation efficiency and poor selectivity in chlorohydrocarbon pollution treatment are solved.

This invention discloses a method and apparatus for enhancing microbial catalytic degradation of chlorinated hydrocarbons in groundwater using a pulsed electric field, relating to the field of groundwater pollution remediation technology. By introducing microbial stimulants, including nutrients and electron donors, into the polluted groundwater area to enhance the activity of functional microorganisms, and applying a pulsed electric field to create a dynamic potential gradient, the method promotes pollutant migration and microbial metabolism, achieving a stepwise degradation of chlorinated hydrocarbons from highly chlorinated intermediates to low-chlorinated intermediates and ultimately to non-toxic alkanes. Simultaneously, monitoring data such as pollutant concentration, environmental parameters, and microbial activity are collected, and the pulsed electric field parameters and stimulant addition strategy are dynamically adjusted based on this data to match external stimulation with dechlorination requirements. This promotes the continuous and efficient conversion of highly chlorinated hydrocarbons to low-chlorinated hydrocarbons and ultimately to non-toxic alkanes, improving electron utilization efficiency and system stability, shortening the remediation cycle, and reducing energy consumption and the risk of secondary pollution.

The present invention belongs to the field of environmental microbial technology, and specifically discloses two trichloroethylene-assimilating and degrading bacteria, Cytobacillus oceanisediminis SH48 and Lysinibacillus fusiformis SH13, and their applications. The strains of Cytobacillus oceanisediminis SH48 and Lysinibacillus fusiformis SH13 are deposited in the China Center for Type Culture Collection, with the deposit numbers being CCTCC M 2023370 and CCTCC M 2023371 respectively, and the deposit date being March 21, 2023. The growth of these two strains has low requirements for environmental conditions. They can use trichloroethylene as the sole carbon source and energy source for their own growth without adding additional growth substrates, effectively degrade chlorinated hydrocarbon pollutants, release inorganic free chloride ions, and there is no accumulation of toxic intermediate products. The strain or the microbial agent prepared therefrom can be used for the bioremediation of the soil or groundwater in industrial polluted sites contaminated with chlorinated hydrocarbons, and has important application prospects.