19 results about "Antigen assays" patented technology

The invention provides monoclonal antibodies of H protein and N protein of canine distemper virus and application thereof in preparation of a fluorescent microsphere antigen detection test strip. The monoclonal antibody disclosed by the invention is characterized in that (1) the monoclonal antibody contains a heavy chain variable region CDV-N-3A11-VH and a light chain variable region CDV-N-3A11-VL; the amino acid sequence of the CDV-N-3A11-VH is as shown in the 1st to 116th sites of SEQ ID No.1 in a sequence table; the amino acid sequence of the CDV-N-3A11-VL is as shown in the 1st site to the 113th site of SEQ ID No.2 in a sequence table. (2) a heavy chain variable region CDV-H-1G5-VH and a light chain variable region CDV-H-1G5-VL are contained; the amino acid sequence of the CDV-H-1G5-VH is as shown in the first site to the 114 site of SEQ ID No.3 in a sequence table; the amino acid sequence of the CDV-H-1G5-VL is as shown in the 1st site to the 1st site and the 1st site to the 1st site of SEQ ID No.4 in a sequence table. The canine distemper virus fluorescent microsphere antigen test strip composed of the two antibodies is suitable for detecting canine distemper viruses in samples such as eye and nose swabs and virus cultures, is high in sensitivity and good in specificity, and can be used for early diagnosis of canine distemper virus infection.

The present disclosure discloses a detection kit and a preparation method thereof and a detection method for novel coronavirus, and relates to the technical field of biomedicine, including an antigen test strip. The antigen test strip includes a substrate, bibulous paper, immune nitrocellulose membrane, and immune microsphere pad. The immune microsphere pad, the immune nitrocellulose membrane and the bibulous paper are pasted on the substrate. The immune microsphere pad is coated with latex microsphere-labeled novel coronavirus SARS-CoV-2 monoclonal antibody 1. The immune nitrocellulose membrane is provided with a test line coated with novel coronavirus SARS-CoV-2 monoclonal antibody 2 and a quality control line coated with goat anti-mouse IgG polyclonal antibody. The present disclosure uses latex particles as labeled tracer, and uses antigen-antibody reaction and lateral chromatography to detect and analyze targets, having advantages of convenience and swift.

The present invention discloses a method for automatically distinguishing the results of multi-directional and multi-scale new coronavirus antigen detection. The method constructs a priori structural elements, performs a priori flexible morphological operator filtering on the original image of the antigen test kit test result, calculates the a priori flexible morphological gradient, extracts the edge of the antigen test kit, extracts the test paper area to be distinguished, eliminates interference such as reflective areas, and locates potential areas in sequence to perform pre-detection of letter discrimination areas; performs discrimination letter modeling in the angle dimension to form a training set, combines samples into a tensor form to form a sample feature matrix; converts the RGB color image to the HSV color space, expands the letter image block to extract features, and constructs a feature matching operator to classify the letters C and T; performs color histogram statistics on the test paper area to be distinguished, calculates the color segmentation threshold, and detects the red discrimination mark; and splices the letter classification result and the red discrimination mark detection result to give the discrimination result of the antigen test.

1. Name of the product of this design: Antigen detection device. 2. Purpose of this product design: capable of performing multiple antigen tests. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. Description of other situations that need explanation: The usage state reference diagram shows four antigen test boxes that can be connected to the antigen detection device.

This invention discloses a method and system for interpreting the results of multi-antigen test strips based on artificial intelligence visual recognition, belonging to the field of artificial intelligence detection technology. The method includes acquiring continuous images of radial multi-antigen test strips, performing quality assessment on the images, and selecting target analysis images; locating the center point of the test strip card based on the target analysis images, identifying the direction and number of each detection arm, establishing a polar coordinate system, and performing geometric correction on the images according to the polar coordinate system; segmenting the fan-shaped regions of each detection arm according to the polar coordinate system, locating the control line and test line in each detection arm, and obtaining a set of regions of interest for the strips; extracting the visual features of the set of regions of interest for the strips, and normalizing the test line features based on the corresponding features of the control line in the same detection arm. This invention constructs a polar coordinate correction and same-arm normalization mechanism, integrating artificial intelligence interpretation and a time-series stabilization mechanism to achieve high-precision and interpretable intelligent interpretation of radial multi-antigen test strips.

The invention belongs to the technical field of biomedicine, and particularly discloses a squamous epithelial cell carcinoma antigen determination kit and application, the squamous epithelial cell carcinoma antigen determination kit comprises an anti-reagent A, an anti-reagent B and a magnetic particle reagent, the anti-reagent A is a biotin-labeled squamous epithelial cell carcinoma antigen antibody; the anti-reagent B is an alkaline phosphatase labeled squamous cell carcinoma antigen-antibody; the squamous cell carcinoma antigen determination kit can effectively detect the concentration of squamous cell carcinoma antigen (SCC) in serum and plasma samples, is mainly used for treatment monitoring of cervical cancer, non-small cell carcinoma and the like clinically, and has a wide application prospect.

A disclosed lanthanide fluorescent antigen kit based on monkey pox virus detection comprises an antigen kit, the interior of the antigen kit is fixedly connected with a fixed placing frame, the top of the fixed placing frame is provided with two concave grooves, and each concave groove is internally provided with a plurality of antigen reagent sampling mechanisms; a clamping mechanism is arranged at a position opposite to the bottom end of the antigen reagent sampling mechanism; and an ejection mechanism is arranged at the bottom end of the clamping mechanism, the clamping mechanism comprises a clamping round frame fixedly connected with the bottom end of the interior of the concave groove, and a plurality of fixed rectangular shells are arranged at the top end of the interior of the clamping round frame. According to the scheme, a lanthanide fluorescent antigen reagent test tube for detecting the monkey pox virus is conveniently placed in a clamping round frame on the fluorescent antigen kit, and the lanthanide fluorescent antigen reagent test tube for detecting the monkey pox virus is extruded and clamped by a movable extrusion roller through telescopic matching of rectangular strips in a plurality of fixed rectangular shells.

The invention relates to a colloidal gold detection method and system of an antigen kit. The method comprises the following steps: acquiring an image of colloidal gold to be detected; obtaining the particle size range of the to-be-detected colloidal gold according to the image of the to-be-detected colloidal gold; comparing the particle size range of the to-be-detected colloidal gold with the standard particle size to obtain an initial detection result of the to-be-detected colloidal gold; if the initial detection result of the to-be-detected colloidal gold is qualified, carrying out secondary detection on the to-be-detected colloidal gold; the secondary detection comprises the following steps: respectively carrying out DLS particle size analysis, Zeta potential detection and electron microscope imaging on the to-be-detected colloidal gold to obtain particle size distribution uniformity, Zeta potential and particle size morphology regularity of the to-be-detected colloidal gold so as to obtain a detection result of the to-be-detected colloidal gold. The system and the equipment are used for executing the method. According to the invention, the artificial subjective influence in the colloidal gold quality inspection process can be reduced, and the accuracy and reliability of the colloidal gold quality inspection process are improved, so that the product quality of the antigen kit is improved.

The application discloses an aptamer-nano gold test strip for screening human pulmonary tuberculosis and an application thereof, and belongs to the technical field of biological detection. The application successfully constructs an aptamer-nano gold test strip for screening human pulmonary tuberculosis, realizes rapid detection of a pulmonary tuberculosis marker ESAT-6 antigen, adopts mercapto-modified aptamer to modify nano gold to form stable gold label probes, adopts a mixed solution composed of a biotin-modified aptamer solution and a streptavidin solution, and constructs an ESAT-6 antigen test strip by using an ESAT-6 antigen solution. The constructed test strip has good sensitivity in ESAT-6 antigen detection, has good detection effect in the antigen concentration range of 100 pg / mL-500 pg / mL, and has good specificity, stability, repeatability and accuracy.

The utility model discloses an automatic sample adding device for cell antibody detection, and particularly relates to the technical field of medical scientific research instruments, the automatic sample adding device comprises a supporting plate, a top plate is fixedly arranged on the front side of the supporting plate, a first electric push rod is fixedly arranged at the bottom of the top plate, and a U-shaped plate is fixedly arranged at the output end of the first electric push rod; a sample adding assembly is arranged at the bottom of the U-shaped plate, a sliding frame is fixedly arranged at the top of the supporting plate, a sliding block is arranged at the top of the sliding frame in a sliding mode, a glass dish is rotationally connected to the top of the sliding block, two concave blocks are arranged on one side of the glass dish, the two concave blocks are fixedly connected with the sliding block, and a moving assembly is arranged on the front side of the sliding frame. According to the utility model, the sample adding assembly is arranged, so that the functions of more accurately controlling the suction amount, the injection speed and the injection force of an antigen sample are realized, and the problem that the test result is easily influenced because the force and the dosage of sample adding each time are generally different when a cell antigen test is manually detected is solved.

The invention discloses preparation of a feline distemper virus VP2 protein monoclonal antibody and application of the feline distemper virus VP2 protein monoclonal antibody in preparation of a fluorescent microsphere antigen detection test strip. The monoclonal antibody is as shown in 1) and 2), wherein the monoclonal antibody contains a heavy chain variable region FPV-VP2-2A2-VH and a light chain variable region FPV-VP2-2A2-VL; a heavy chain variable region FPV-VP2-2D9-VH and a light chain variable region FPV-VP2-2D9-VL are included, and the heavy chain variable region FPV-VP2-2D9-VH and the light chain variable region FPV-VP2 1) the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.1 in a sequence table; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2 in the sequence table. 2) the amino acid sequence of the heavy chain variable region is shown as SEQ ID No.3 in the sequence table; and the amino acid sequence of the light chain variable region is as shown in SEQ ID No.4 in the sequence table. The feline plague fluorescent microsphere antigen test strip composed of the two antibodies can be used for detecting feline plague antigens, is high in sensitivity and good in specificity, and can be used for early diagnosis of feline plague infection.

A method of assessing canine expression profiles of miRNA markers and using predictive classification algorithms to diagnose heartworm infection. The analysis of the expression level of specific miRNAs enables early detection of the heartworm infection, including low-burden and male-only infections, which are often missed by antigen tests.

The application discloses a novel coronavirus colloidal gold antigen detection method which can be supervised, and comprises the following steps: S1, preparing a novel coronavirus colloidal gold antigen test paper card with an interference band, and storing band position information; S2, a user tests a novel coronavirus antigen and uploads personal information and test paper card test result information; and S3, feeding back an antigen detection result according to the band position information and the test paper card test result information, and storing the personal information and the antigen detection result. The application has the beneficial effect that the user cannot directly read the antigen detection result by preparing the novel coronavirus colloidal gold antigen test paper card with the interference band, and must upload the personal information and the test result, obtain the antigen detection result by searching a record module and comparing the band position information, and store the personal information and the antigen detection result to realize supervision on the antigen detection result, thereby avoiding the defect that the antigen detection result cannot be supervised in the prior art.

The application belongs to the technical field of biomedicine, and specifically discloses a squamous cell carcinoma antigen determination kit and application, which comprises anti-reagent A, anti-reagent B and magnetic micro-particle reagent, wherein the anti-reagent A is a biotin-labeled squamous cell carcinoma antigen antibody; the anti-reagent B is an alkaline phosphatase-labeled squamous cell carcinoma antigen antibody; the squamous cell carcinoma antigen determination kit can effectively detect the concentration of squamous cell carcinoma antigen (SCC) in serum and plasma samples, and is mainly used for the treatment monitoring of cervical cancer, non-small cell carcinoma and the like, and has a wide application prospect.

To provide a method, a composition and a system for testing a fecal sample containing a small amount of human fecal specimen for a plurality of different marker analytes, for example, a plurality of different human proteins, nucleic acids, and other molecular marker analytes.SOLUTION: There is provided a method for characterizing a human fecal specimen, the method including: (i) providing a dispersed sample containing a small amount of a human fecal specimen dispersed in a predetermined amount of a stabilizing solution; and (ii) analyzing the dispersed sample for various amounts of a plurality of different marker analytes.SELECTED DRAWING: None

The utility model discloses an antigen dilution assisting and temporary storage device which comprises a box body, a plurality of supporting pieces are correspondingly arranged on the inner wall of the box body, a placing plate is mounted on the supporting pieces, and a plurality of groups of through holes for inserting antigen test tubes are formed in the placing plate; dilution volume values corresponding to the through holes in each transverse row are engraved on the top surface of the placing plate, and dilution concentration values corresponding to the through holes in each longitudinal row are engraved on the top surface of the placing plate; the box body located below the placing plate is filled with cooling liquid, and cooling assemblies used for cooling the cooling liquid are installed on the two sides of the box body. According to the device, the risk of misoperation in the dilution process of the carbohydrate antigen C199 sample can be reduced, and meanwhile, the activity of the sample can be ensured in the experiment process, so that a relatively perfect detection result can be obtained.

The utility model relates to the field of antigen kit pressing, and discloses antigen kit pressing and assembling equipment which comprises a base, a rotating rod is rotatably connected to the inner wall of the upper end of the base, a worm is fixedly connected to the outer wall of the bottom end of the rotating rod, a worm gear is meshed with the outer wall of the right end of the worm, and the worm gear is fixedly connected to the outer wall of the right end of the worm. A rotating shaft is fixedly connected to the inner wall of the worm wheel, a gear is fixedly connected to the outer wall of the rotating shaft, a toothed plate is meshed with the outer wall of the bottom end of the gear, and a clamping block is fixedly connected to the outer wall of the upper end of the toothed plate. The rotating rod in the base is rotated to drive the worm to rotate, the worm rotates to drive the worm gear to rotate in a meshed mode, the worm gear drives the rotating shaft to rotate, and therefore the gear on the rotating shaft rotates, and when the gear rotates, the two symmetrically-arranged toothed plates meshed with the gear slide in the opposite directions along the inner wall of the base. And then the clamping block at the upper end of the toothed plate is driven to move, so that the die holder can be quickly centered and fixed.

The utility model discloses a hepatitis virus antigen rapid detection card which comprises a box body, a plurality of moving plates are slidably mounted in the box body through moving grooves, a sliding rod is slidably mounted on the inner wall of each moving groove through a sliding groove, a sliding sleeve is slidably mounted on the outer wall of each sliding rod, a spring is mounted on the outer wall of each sliding rod, and the moving plates are slidably mounted in the box body through sliding grooves. The two ends of each spring are elastically connected with the inner wall of the corresponding sliding groove and the outer wall of the corresponding sliding sleeve correspondingly, and the upper end face of each sliding sleeve is fixedly connected with the outer wall of the corresponding movable plate through a connecting mechanism. Through the arrangement of the spring, the sliding block, the connecting plate and other components, when the fixing of the moving plate is released, the spring can release elastic potential energy to drive the sliding sleeve to slide on the outer wall of the sliding rod, and then the sliding sleeve drives the moving plate to move outwards in the moving groove through the connecting plate, so that the exposed area of the sampling plate can be increased; and a sample can be contacted with a chemical reagent on the sampling plate more easily and fully, so that the detection efficiency can be improved.

The utility model relates to the technical field of kits, and discloses a quick buckling structure of an antigen kit, which comprises a kit body, a buckling mechanism is arranged on the kit body, an auxiliary mechanism is arranged on the buckling mechanism, the buckling mechanism comprises a mounting frame, the mounting frame is slidably connected to the inner wall of the bottom of the kit body, and the auxiliary mechanism is arranged on the mounting frame. A storage rack is slidably connected to the inner wall of the top of the mounting frame, a shifting piece is slidably connected to the inner wall of one side of the mounting frame, an arc-shaped block is fixedly connected to the outer wall of one side of the shifting piece, a telescopic spring is fixedly connected to the outer wall of the side, away from the arc-shaped block, of the shifting piece, and a limiting opening is formed in the outer wall of one side of the kit body. According to the utility model, by arranging the structures such as the shifting piece and the arc-shaped block, the problems in the prior art that the antigen expression level of tumor cells needs to be quantitatively analyzed and detected, various reagents are needed, multiple groups of detection results are obtained, and a plurality of reagent containers need to be used for sub-packaging, so that the carrying and the storage are inconvenient are solved.