Three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol and preparation method thereof
A technology of thiamphenicol and florfenicol, which is applied in the field of immunological detection, can solve the problems of high cost, high detection cost, and is not suitable for low-cost detection of enterprises, and achieves the effects of simple operation and rapid detection.
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Embodiment 1
[0025] Such as figure 1 As shown, a three-in-one colloidal gold immunochromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol, including polyvinyl chloride PVC backing 1, and the front end of polyvinyl chloride backing 1 is set The sample pad 2, the sample pad 2 is connected to the front end of the nitrocellulose membrane 4 through the binding pad 3, and the rear end of the nitrocellulose membrane 4 is connected to the water-absorbing pad 5; the binding pad 3 contains a colloidal gold-labeled monoclonal antibody; the nitrocellulose The prime film 4 is coated with chloramphenicol sodium succinate-BSA detection line T line 6 and goat anti-mouse IgG control line C line 7 .
Embodiment 2
[0027] The preparation method of the three-in-one colloidal gold immunochromatography test strip for detecting thiamphenicol, chloramphenicol and florfenicol, the steps are:
[0028] (1) Preparation and purification of monoclonal antibodies that can simultaneously recognize thiamphenicol, chloramphenicol and florfenicol: the analogue of thiamphenicol, namely 1-(4-aminophenyl)-2- Dichloroethylamine-1,3-propanediol (TAP-NH 2 ) as a hapten, coupled with bovine serum albumin (BSA) by the diazotization method to obtain an immunogen, mixed with Freund's adjuvant to immunize BALB / c mice; take the spleen cells and myeloma cells of qualified immunized mice Sp2 / 0 fusion, after three times of subcloning and indirect competition ELISA screening, a cell line that can stably secrete monoclonal antibodies that can recognize thiamphenicol, chloramphenicol and florfenicol at the same time was obtained. After expanding the culture, Inject into mice to induce ascites; use octanoic acid-saturate...
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