100results about How to "Rapid Field Detection" patented technology

The invention belongs to a railway transportation field and relates to a device for measuring horizontally and vertically dynamic displacement of a high-speed railway track line. The device comprises two baffle boards which are equipped at middle of the measured rail perpendicular to the ground or form an angle of 45 degrees with the ground, two laser displacement sensors, and a portable data processing and displaying device; making use of the baffle board which forms an angle of 45 degrees with the ground, the vertical displacement of the track line is converted into a change amount of a horizontal distance by a proportion of 1:1 equivalently; heads of the two laser displacement sensors are aligned with the two baffle boards to measure the dynamic horizontal displacement and the compound displacement including the horizontal displacement and the vertical displacement when a high-speed train passes; the data measured by the two laser displacement sensors are processed by the portable data processing and displaying device, and then the instantaneous horizontal and vertical displacement during the high-speed train passing the track line can be read directly; The measuring device has the advantages of accurate measured data and high respond speed.

The invention discloses a primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through a RPA-lateral flow assay technology. A primer probe liquid, popular primer and probes are arranged in the kit, wherein the primer probe liquid consists of a popular subtype primer which is used for detecting the foot-and-mouth disease virus through an RPA technology, and the probes, and the popular primer and the probes are in the primer probe liquid. The primer, the probe and the kit, which are disclosed by the invention, adopt the RPA-Lateral flow assay technology to establish a method for rapidly detecting the FMDV of cattle for the first time, and through the evaluation of specificity and evaluation of sensitivity, the sensitive, reliable and new method can be used for a clinical detection on site of the FMDV. Through the combination of the primer and the probes, which are disclosed by the invention, the method for detecting the FMDV in the aerosol in a subtype manner through the RPA technology has the advantages of being higher in sensitivity, specificity and repeatability.

The invention discloses a two-dimensional microfluidic paper chip and a preparation method thereof. The two-dimensional microfluidic paper chip, from top to bottom, sequentially comprises a hydrophobic transparent membrane, a hydrophilic paper channel and a hydrophobic substrate, wherein the hydrophilic paper channel and the hydrophobic substrate are detachably connected by a waterproof double-faced adhesive tape, the surface of the hydrophobic transparent membrane is provided with a to-be-tested liquid inlet corresponding to the hydrophilic paper channel, and the preparation method of the paper chip is also very simple and quick. The two-dimensional microfluidic paper chip disclosed by the invention adopts a simple manufacturing method and is easy to carry, and can be used for rapid field test.

The invention discloses a method for quickly detecting heavy metal in water quality based on an ultra-infiltration microchip and belongs to the fields of material preparation and chemical examinationand analysis. The method comprises the following steps: firstly, reacting hydrophobic silica particles with fluorosilane in an ethanol solution to obtain superhydrophobic silica suspension; secondly,coating one side of a double-sided adhesive tape with the suspension to obtain superhydrophobic surface; finally, carrying out plasma etching in a designated area to obtain superhydrophilic microporesand an ultra-infiltration chip; pasting the ultra-infiltration chip on a specific substrate instrument by using the stickiness of the adhesive tape. By use of the ultra-infiltration chip, a color-producing reagent can be concentrated and enriched on the superhydrophilic micropores; the prepared ultra-infiltration chip can fix a detected solution on superhydrophilic sites by simple dipping operation, and colorimetric detection is realized by using a detection reagent on the hydrophilic sites. According to the method, the problems of high cost of a traditional apparatus analysis method and troublesome operation of a chemical analysis method are solved; the method is suitable for quick field detection of an aqueous solution.

The invention provides a three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol and a preparation method thereof, and belongs to the technical field of immunological detection. The preparation method comprises two parts, namely, the preparation of a monoclonal antibody and the preparation of a colloidal gold chromatographic test strip, wherein the monoclonal antibody can be used for recognizing thiamphenicol, chloramphenicol and florfenicol at the same time and is high in sensitivity; the colloidal gold chromatographic test strip comprises a polyvinyl chloride backing; a sample pad is arranged at the front end of the polyvinyl chloride backing and is connected with the front end of a nitrocellulose membrane; the rear end of the nitrocellulose membrane is connected with a water absorbing pad; the monoclonal antibody marked by colloidal gold is used as a combining pad; the nitrocellulose membrane is sequentially wrapped with a chloramphenicol sodium succinate-BSA (Bovine Serum Albumin) antigen test ray T and a goat-anti-mouse IgG control ray C. The three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol is fast to detect, and the detection needs only 3 to 5 minutes; the test strip is convenient to carry, and suitable for detection on site; the operation is simple and convenient and does not need a professional technical person.

A method for detecting diethylstilbestrol belongs to the field of analytical chemistry, wherein the method includes the followings: pre-treating the sample to be detected, preparing the sample solution and then carrying out the following treatments: adding sample solution at one end point of the paper chromatography strip; placing the paper chromatography strip in the normal hexane-ethanol developing solvent; taking out the paper chromatography strip when the front end of the developing solvent is adjacent to the front end of the paper chromatography strip; air-drying, wherein the normal hexane-ethanol developing solvent by volume includes: 1 to 20 parts of normal hexane, 0.1 to 5 parts of ethanol; spraying the potassium ferricyanide-ferric trichloride-H2O2 on the chromatography strip; color displaying, wherein the potassium ferricyanide-ferric trichloride-H2O2 is as follows: mixing the 0.01 to 5 mol/L aqueous solution of the potassium ferricyanide and the 0.01 to 5 mol/L aqueous solution of ferric trichloride evenly; then adding 0.001% to 5% of the H2O2 by volume 1 to 1; and the volume of the H2O2 is 0 to 10% of the total volume of the solution. The invention has the advantages of simple method and device, fast detecting speed and low detecting cost, and can be used for fastly detecting the DES in food on the spot.

The invention provides a real-time monitoring system for a bottom surface of a bridge. The real-time monitoring system comprises a rail mechanism which is arranged at the bottom surface of the bridge, and a detection mechanism which is arranged on the rail mechanism and is used for detecting the actual condition of the bottom surface of the bridge; a main rail is arranged on the bottom surface of the bridge and a secondary rail is arranged at the lower part of a bridge opening and the detection mechanism arranged on the main rail is used for bearing a secondary detection mechanism; the arranged main rail is higher than the vertical height of the secondary rail; the detection mechanism moves on the main rail and the secondary detection mechanism is laid on the secondary rail along the movement direction of the main rail, so that a monitoring test is carried out in all directions and a phenomenon that a detection period is long is avoided; and acquired data information is returned back to a central control room and is subjected to rapid comparison and analysis, so that the problems of the bottom of the bridge are rapidly compared.

The invention discloses a Newcastle disease novel visualized room temperature recombinase ploymerase amplication (RT-RPA) nucleic acid test strip detection kit and applications. The kit contains the nucleotide sequences of primers and a probe which are shown in SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7, and a nucleic acid detection test strip. The application method of the kit includes the following steps: configuring an RT-RPA reaction system, and performing a normal temperature and constant temperature reaction; and directly performing interpretation on a product obtained by the constant temperature reaction after the product is detected by the nucleic acid detection test strip, a positive result appearing two red strips, one being located in a detection area, and the other one being locatedin a quality control area, and a negative result only appearing one red strip in the quality control area, and no red strips appears in the detection area. The method for rapidly detecting Newcastledisease virus can be established by adopting an RPA-nucleic acid test strip technology; and the kit and the method are simple in operation, high in specificity and safe and pollution-free, and are easy to observe reaction results and suitable for clinical site detection.

The invention discloses a test strip kit for detecting nucleic acid of classical swine fever virus through room-temperature recombinase polymerase amplification (RT-RPA) and application thereof. The kit comprises primer pairs with nucleotide sequence as shown in the claim 1 and a nucleic acid test strip. An application method of the kit comprises the following steps: firstly a RT-RPA reaction system is configured for normal-temperature constant-temperature reaction; after detected with the nucleic acid test strip, a product obtained after the constant-temperature reaction directly undergoes interpretation: a positive result is two red strips with one strip positioned in a detection area and the other strip positioned in a quality control area; a negative result is a red strip in the quality control area with no red strip in the detection area. The RPA-nucleic acid test strip technology is adopted for the first time to establish a method for rapid detection of CSFV. The kit of the invention is simple to operate and has high specificity. Reaction results are easy to observe. The kit is safe and pollution-free, and is very suitable for clinic field test.

The invention discloses a method for rapid and visual paper sensing detection of heavy metal silver ions, and belongs to the technical field of heavy metal detection. The method specifically comprisesthe following steps of synthesis of nano gold, synthesis of tetra-(4-pyridyl) zinc protoporphyrin self-assembly body; preparation of a nano gold and nano porphyrin composite paper sensing device substrate, manufacturing of a silver ion standard colourimetric card, and comparison of the concentration, detected by the colourimetric card, of the silver ions in a to-be-tested sample. The method for detecting the silver ions based on the nano gold and nano porphyrin composite paper sensing substrate has the characteristics of easy preparation, rapid field detection, low cost, high response speed,high sensitivity and high selectivity, and the nano gold and nano porphyrin composite paper sensing substrate has a specific reaction to the silver ions, and can be used for detection of the silver ions in complex substrate water samples.

The invention relates to a molecular imprinting photonic crystal detecting card and application thereof. Molecular imprinting pre-polymerization liquid is poured into a photonic crystal template; after polymerization, template molecules are washed away to obtain the molecular imprinting photonic crystal detecting card. The molecular imprinting photonic crystal pesticide residue fast detecting cardrelated by the invention has specific selectivity on target pesticide molecules; the interference of other pesticide molecules in complicated test environments can be avoided; the surface structure color can respond the outside stimulation; the naked eye visibility is realized; through contrast with a standard color card, the content of methomyl in an object to be tested can be fast obtained. Theprepared molecular imprinting photonic crystal detecting card provides an accurate, sensitive and fast novel method for detecting the methomyl pesticide residue; the reference is also provided for the development of other kinds of pesticide residue detecting technologies.

The invention discloses a primer and a probe for detecting BPIV3 (Bovine parainfluenza virus type 3) aerosol through a RPA (Recombinase Ploymerase Amplification)-lateral flow assay, a kit and a detection method. The kit contains primer probe liquid formed by a RPA primer and a probe for detecting the BPIV3, wherein the forward primer sequence is shown as SEQ ID No.1; the reverse primer sequence isshown as SEQ ID No.2; the probe sequence is shown as SEQ ID No.3. The used primer probe group has a good RPA effect; the strip specificity is high; no cross reaction with other viruses exists; strongpositive reaction exists in a detection region; the detection sensitivity is enhanced. The RPA-lateral flow assay technology is used for building a BPIV3 fast detection method for the first time; through specificity and sensitivity evaluation, the method can be used for clinical field detection; a flexible and reliable novel method is provided for BPIV3 field detection.

The invention relates to a method of ultrafast detection of antibiotic substances by combined voltage driven solid phase microextraction-raman spectroscopy. The method comprises the following steps: taking a nano golden particle wrapped porous silver wire as a working electrode, a saturated calomel electrode as a reference electrode and a platinum electrode as a counter electrode to form an electrode system, putting the electrode system in a water sample to be detected, applying -0.05 to -0.2V voltage to the electrodes, quickly enriching the antibiotic substances in the water sample onto the working electrode due to voltage driving to achieve in-situ solid phase microextraction, and performing exciting irradiation on the surface of the working electrode through a raman spectrometer for raman spectroscopy. The method increases a diffusion rate of the substances to be detected in the water sample; the nano golden particle wrapped porous silver wire is specific to the antibiotic substances, so that the antibiotic substances are enriched onto the working electrode directionally selectively; the enrichment detection speed is greatly increased; and actual applications in quick field detection and water sample pollutant surveillance are achieved.

The invention discloses a method for improving a Sudan red I detection kit and belongs to the field of analytic chemistry. According to the method, a sample is pretreated to selectively enrich Sudan red I by a magnetic solid-phase extraction mode and then is detected by the Sudan red I detection kit. The method comprises the following steps of accurately weighing the sample, putting the sample into a container, adding an extraction solvent, oscillating and standing the container, and taking supernate as upper sample liquid; adding the upper sample liquid into a container holding a magnetic solid-phase extraction material, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding normal hexane, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding acetone, oscillating the container, dropwise adding the supernate on a sample application plate, and drying the sample application plate in a blowing manner; and executing other operations according to an operating manual of the detection kit. According to the method disclosed by the invention, the Sudan red I in the sample can be effectively and quickly enriched through magnetic solid-phase extraction, so that the detection limit of the kit is reduced by 3 times, and the phenomena of false positive and missing detection are avoided to an extremely large extent. The method has the advantages of simplicity, quickness, easiness in operation and the like, and is suitable for field test, and solvents can be saved.

The invention relates to the field of biotechnology and particularly relates to a LAMP (loop-mediated isothermal amplification) primer group and a kit for detecting influenza A virus (IAV). The primergroup comprises one or more of the following 29 primer groups: primer groups for amplifying segments of the regions H1-H18 of IVA and primer groups for amplifying segments of the regions N1-N11 of IVA. The invention also provides a kit for detecting IAV. The primer group provided by the invention has high sensitivity and high specificity; the kit prepared from the primer group can be used for quickly and accurately detecting the IAV contained in a to-be-detected sample and can be applied to the typing detection and identification of the IAV type in the to-be-detected sample. According to theprimer group and kit provided by the invention, the false positive and false negative phenomena are avoided in a process of adopting artificial RNA for control detection. The primer provided by the invention has high sensitivity and specificity; the provided visual kit brings great convenience to field detection; quick and accurate detection of IAV can be realized by preparing a kit from the primer.

The invention discloses a detection test paper for Francisella tularensis antibody, a preparation method thereof, a detection method utilizing the detection test paper and a quantitative detection system, the rapid detection method for the Francisella tularensis antibody is established by utilizing the colloid gold labeling technique and double antibody sandwich immunochromatographic technology, and the established colloid gold immunochromatographic technology for detecting the Francisella tularensis antibody can rapidly, sensitively, specifically and accurately detect the Francisella tularensis antibody in a sample, realize quantitative detection and be applicable to field rapid detection.