10results about How to "Rapid Field Detection" patented technology

The invention belongs to the technical field of colorimetric chemical sensors, and particularly relates to a preparation and application method of a smartphone-assisted surface molecular imprinting colorimetric sensor for detecting norfloxacin, magnetic Fe3O4 (at) KH540 nanoparticles are taken as a matrix, magnetic norfloxacin surface molecular imprinting nano-enzyme MMIPs is prepared by adopting a surface molecular imprinting technology, then the MMIPs, H2O2 and 3, 3 ', 5, 6, 7-tetramethyl-3, 5, 6-tetramethyl-3, 5, 6-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3 The colorimetric sensor for detecting norfloxacin is constructed by carrying out a chromogenic reaction on 3, 3 ', 5, 5'-tetramethyl benzidine TMB and cooperating with a smartphone. The sensor has specific recognition selectivity and binding affinity for NOR, is simple to operate, high in detection speed and low in cost, does not need expensive instruments, and can be suitable for field analysis.

The invention discloses a method for rapid broad-spectrum detection of salmonella, and relates to the technical field of bacterial detection. Comprising the following steps: (1) preparing a labeled SERS probe; (2) preparing a Fe3O4 (at) Au (at) Ag magnetic composite material; (3) combining Fe3O4 (at) Au (at) AgNPs with Ag (at) 4MBA (at) AuNPs to construct an aptamer sensor; and (4) carrying out SERS detection on the salmonella. According to the novel surface enhanced Raman scattering (SERS) aptamer sensor constructed on the basis of noble metal nanoparticles and a magnetic composite material, according to the inverse proportion relation that the higher the salmonella concentration is, the weaker the Raman signal is generated according to the principle of a competition law, the ultrasensitive rapid field detection is carried out on different salmonella.

The invention relates to a finger-pressing type nucleic acid detection micro-fluidic chip, which comprises a bottom layer, a flow channel layer, a finger-pressing layer and a thin film layer, the flow channel layer comprises an LAMP reaction chamber, a CRISPR reaction chamber, a water storage chamber, a Tesla valve, a finger-pressing chamber and a test paper reaction chamber, the LAMP reaction chamber comprises a nucleic acid extraction-free reaction system based on LAMP, the CRISPR reaction chamber comprises a CRISPR-based cas12b reaction system, and the test paper reaction chamber comprises a Tesla valve, a finger-pressing chamber and a test paper reaction system. The water storage chamber is mainly used for diluting a mixed system, and the Tesla valve is used for preventing crosstalk of the two reaction systems; the finger pressing layer comprises an LAMP reaction chamber button and a CRISPR reaction chamber button, when the LAMP reaction chamber button is pressed, liquid in the LAMP reaction chamber is pumped into the CRISPR reaction chamber, when the two buttons are pressed at the same time, a reaction mixing system is pumped into the water storage chamber to be mixed, and then the reaction mixing system is further pumped into the test paper reaction chamber. The chip can be widely applied to the field of rapid diagnosis of nucleic acid molecules, the detection cost is reduced, manual operation is simplified, and the detection efficiency is improved.

The application discloses an aggregation-induced emission metal organic framework, a preparation method thereof and fluorescent / colorimetric dual-mode intelligent detection for trace HCl. 2+ The aggregation-induced emission metal organic framework is prepared by providing Zn(NO3)3.6H2O as Zn 2+ , imidazole-2-formaldehyde and an AIE molecule HDBB as organic ligands, and through a solvothermal method to obtain ZIF-90-HDBB, which can change the aggregation state fluorescence from yellow green to orange red after being contacted with HCl gas; the ZIF-90-HDBB is uniformly loaded on cellulose filter paper to obtain a paper-based sensor for portable detection of HCl, and through combination with a color recognition APP of a smart phone, the HCl can be rapidly and sensitively detected on site in a fluorescent / colorimetric dual mode. Since the HDBB molecule is prone to protonation under acidic conditions, the fluorescence color changes, and therefore the material can be accurately and efficiently used for detection of acidic gas, and the material can be recycled.

The invention relates to the technical field of medical detection, and discloses a canine coronavirus antigen fluorescence lateral chromatography detection method which comprises the following steps: A, coupling a canine coronavirus paired antibody with carboxyl fluorescent microspheres to form fluorescently-labeled antibody microspheres; b, spraying the fluorescently-labeled antibody microspheres on a conjugate pad, and drying the conjugate pad; c, respectively coating the nitrocellulose membrane with a canine coronavirus antibody and goat anti-canine IgG to form a test line and a control line; and D, assembling the sample pad, the microsphere combination pad, the nitrocellulose membrane and the water absorption pad in sequence, and cutting into a test strip with the width of 4mm for detection. According to the detection method, the influence of interfering substances in the sample can be reduced, good specificity and stability are achieved, meanwhile, the detection design enables the detection method to have a simple and convenient operation process, and complex experimental equipment is not needed. The test strip is relatively simple and convenient to assemble and use, so that veterinarians or related workers can quickly carry out on-site detection.

The invention relates to the field of material mechanical property testing, and provides an in-service material property detection system based on an indentation method, comprising: an excitation module for applying a composite dynamic excitation load to a pressure head when the spherical pressure head is pressed into a to-be-detected material; the signal acquisition module is used for synchronously acquiring a dynamic load signal and a dynamic displacement signal in the pressing-in process of the pressing head; the data processing module is used for calculating an instantaneous phase difference according to the dynamic load signal and the dynamic displacement signal, constructing an evolution curve of the instantaneous phase difference along with the change of the pressure depth, and extracting a first characteristic depth, a second characteristic depth and a phase hardening index from the evolution curve; and the performance resolving module is used for calculating the elastic modulus and the yield strength of the to-be-tested material according to the first characteristic depth, the second characteristic depth, the phase hardening index and the mapping relation model. According to the method, the plastic hardening constitutive relation of the material does not need to be preset, rapid and micro-damage detection of the tensile property of the material can be achieved through single-time micron-order pressing-in, and the method is particularly suitable for field evaluation of in-service equipment.

This invention provides a colorimetric detection method for quercetin based on NH2-MIL-101-CuCo nanozymes. The method includes: preparing a first mixture by mixing NH2-MIL-101-CuCo nanozymes, TMB solution, and hydrogen peroxide solution with the sample to be tested, and a second mixture by mixing with a quercetin standard; reacting the mixture under the same reaction conditions to obtain absorbance values ​​A1 and A0 at predetermined wavelengths; determining the quercetin content in the sample based on A1 and linear regression curves of different concentrations of quercetin; the nanozyme is synthesized in one step via a hydrothermal method using copper chloride dihydrate, cobalt chloride hexahydrate, 2-aminoterephthalic acid, and sodium hydroxide. The method utilizes hydrogen bonds formed by the active hydrogen atoms in the nanozyme and the hydroxyl groups in quercetin to specifically recognize and adsorb quercetin, thus enabling the detection of quercetin.

The utility model discloses a detection device for high temperature circulating acid pump dry gas seal, including nitrogen gas source, PVC hose, glass rotor flowmeter and connecting assembly, PVC hose includes hose A and hose B, both ends of hose A are connected with nitrogen gas source and glass rotor flowmeter's import end through connecting assembly, one end of hose B is connected with glass rotor flowmeter's export end through connecting assembly, and the other end is connected with the dry gas seal interface of the pump body to be detected, the utility model discloses nitrogen in nitrogen gas source is transported to glass rotor flowmeter real -time monitoring nitrogen flow through PVC hose, and then is transported to dry gas seal place and is detected. The leakage condition of dry gas seal is judged through the flow change, ensures in time finding the leakage problem of dry gas seal before installation, avoids the acid pump of taking fault and puts into production line, thereby reduces the failure caused by the seal failure, reduces the production interruption and maintenance cost caused by shutdown maintenance, guarantees the stability and security of production system.

The invention discloses an aggregation-induced emission metal organic framework, a preparation method thereof and application to fluorescence / colorimetric dual-mode intelligent detection of trace HCl. According to the aggregation-induced emission metal organic framework, Zn < 2 + > provided by Zn (NO3) 3.6 H2O, imidazole-2-formaldehyde and AIE molecule HDBB serve as organic ligands, ZIF-90-HDBB is obtained through a solvothermal method, and after the material is in contact with HCl gas, aggregation-state fluorescence of the material can be changed into orange red from yellow green; zIF-90-HDBB is uniformly loaded on cellulose filter paper to obtain the portable paper-based sensor for detecting HCl, and the portable paper-based sensor is combined with a smart phone color recognition APP to realize rapid and sensitive HCl fluorescence / colorimetric dual-mode field detection. As HDBB molecules are easy to generate protonation under an acidic condition to generate fluorescence color conversion, the material can be accurately and efficiently used for detecting acidic gas, and the material can be recycled.

The invention develops a dual-mode optical sensor based on CuO2 nanodots. The dual-mode optical sensor is mainly applied to detection and research of a Warburg effect of gastric cancer. The CuO2 nanodots can catalyze the reaction of o-phenylenediamine (OPD) and hydrogen peroxide (H2O2), so that the color of the solution is changed from colorless to yellow along with the increase of absorbance and fluorescence intensity. Based on the reaction, a dual-mode sensor is designed, and the activity of lactic acid and lactate oxidase is synchronously detected through colorimetry and fluorescence. In the process of catalyzing lactic acid to generate H2O2 by lactic acid oxidase, the generated H2O2 promotes the color of a solution to change remarkably, so that the sensitive detection of the activity of lactic acid and oxidase thereof is indirectly realized. As the Warburg effect of the gastric cancer causes rise of the lactic acid level, the sensor can effectively associate the lactic acid level with the metabolic characteristics of the gastric cancer. In addition, in combination with an RGB colorimetric analysis technology, a smart phone is used for capturing color changes and carrying out image quantitative analysis, a portable, low-cost and high-sensitivity detection system is formed, and the application blank of the CuO2 nanodots in the field is filled up.