100results about How to "Rapid Field Detection" patented technology

The invention relates to a non-labeled electrochemical immune sensor for detecting diethylstilbestrol and a preparation method and application of the sensor. The immune sensor comprises a substrate electrode; and after nanometer gold is deposited on the surface of the substrate electrode, a graphene-diethylstilbestrol-chitosan complex is modified, and non-specific active loci are sealed by bovineserum albumin. The preparation method comprises the following step of: after depositing the nanometer gold on the surface of the substrate electrode, enveloping graphene and the diethylstilbestrol byusing chitosan and fixing to the surface of the electrode. In the immune sensor, on the basis of a competitive mode of immune reaction, the immune reaction is monitored by taking K3Fe(CN)6 as a probeand by differential pulse voltammetry, so the immune sensor can be used for detecting the diethylstilbestrol. The non-labeled electrochemical immune sensor has the characteristics of high sensitivityand specificity, quick on-site detection, low cost and the like; and a detection method is simple and wide in application range, the detection limit can reach 0.1 ng / ml, and the linear range is between 0.5 and 1,500 ng / ml.

The invention belongs to a railway transportation field and relates to a device for measuring horizontally and vertically dynamic displacement of a high-speed railway track line. The device comprises two baffle boards which are equipped at middle of the measured rail perpendicular to the ground or form an angle of 45 degrees with the ground, two laser displacement sensors, and a portable data processing and displaying device; making use of the baffle board which forms an angle of 45 degrees with the ground, the vertical displacement of the track line is converted into a change amount of a horizontal distance by a proportion of 1:1 equivalently; heads of the two laser displacement sensors are aligned with the two baffle boards to measure the dynamic horizontal displacement and the compound displacement including the horizontal displacement and the vertical displacement when a high-speed train passes; the data measured by the two laser displacement sensors are processed by the portable data processing and displaying device, and then the instantaneous horizontal and vertical displacement during the high-speed train passing the track line can be read directly; The measuring device has the advantages of accurate measured data and high respond speed.

The invention relates to the field of biochips, and particularly relates to a surface plasma resonance immunosense chip as well as a preparation method and application thereof. The surface plasma resonance immunosense chip is prepared by the following steps: fixing a shrimp tropomyosin, shrimp tropomyosin monoclonal antibody ascites or a shrimp tropomyosin monoclonal antibody on a solid phase carrier as a bioprobe; generating surface plasma resonance response by utilizing a gold membrane; modifying sulfydryl on the surface of the gold membrane by utilizing a self-assembly monomolecular layer technique; and fixing the probe on the biochip after the chip is activated. The biochip can be applied to detection of the shrimp tropomyosin monoclonal antibody or the shrimp tropomyosin monoclonal antibody ascites, and detection of the allergen (shrimp tropomyosin), has the advantage of simplicity and convenience in operation, rapidity, high flexibility, no need of being labeled, low cost, simple device, no pollution to the environment and the like, and is hopeful to realize the field real-time detection of a great amount of samples.

The invention discloses a primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through a RPA-lateral flow assay technology. A primer probe liquid, popular primer and probes are arranged in the kit, wherein the primer probe liquid consists of a popular subtype primer which is used for detecting the foot-and-mouth disease virus through an RPA technology, and the probes, and the popular primer and the probes are in the primer probe liquid. The primer, the probe and the kit, which are disclosed by the invention, adopt the RPA-Lateral flow assay technology to establish a method for rapidly detecting the FMDV of cattle for the first time, and through the evaluation of specificity and evaluation of sensitivity, the sensitive, reliable and new method can be used for a clinical detection on site of the FMDV. Through the combination of the primer and the probes, which are disclosed by the invention, the method for detecting the FMDV in the aerosol in a subtype manner through the RPA technology has the advantages of being higher in sensitivity, specificity and repeatability.

The invention relates to a one-step ELISA (Enzyme Linked Immunosorbent Assay) method for neomycin (NEO) residues in milk, which belongs to the technical field of immunoassay chemicals. The method comprises the following steps of: coupling NEO with bovine serum albumin (BSA) or ovalbumin (OVA) to synthetize a NEO antigen (immunogen NEO-BSA or coating antigen NEO-OVA) by a glutaraldehyde method; authenticating the NEO-BSA by utilizing an infrared spectroscopy and an SDS-PAGE (Sodium Dodecyl Sulfate-PolyAcrylamide Gel Electrophoresis) method; obtaining NEO antiserum by immunization and detectingthe valence of the NEO antiserum by an indirect ELISA method; and detecting the specificity of the NEO antiserum by the one-step ELISA method. Compared with the indirect competition ELISA method, theinvention greatly shortens the detecting time of the specificity of the NEO antibody, has higher sensitivity, achieves the aim of simply, rapidly and sensitively detecting NEO residues in milk and can be widely used for rapid field detection.

The invention provides an aptamer-modified nanogold colorimetric detection-based analysis method of ibuprofen. The aptamer-modified nanogold colorimetric detection-based analysis method comprises following steps: single strand nucleic acid ibuprofen aptamer is taken as an identification probe of ibuprofen, ibuprofen standard solutions with different concentrations are mixed with gold nanoparticle solutions, single strand nucleic acid ibuprofen aptamer solutions, and salt solutions so as to obtain standard detection solutions of different concentrations; after reaction, an ultraviolet spectrophotometer is adopted to detect light absorption values of obtained systems based on different discoloration degrees of the systems caused by gold nanoparticle coagulation, a standard curve is drawn, and the concentration of ibuprofen of a sample to be detected is determined according to the standard curve. According to the aptamer-modified nanogold colorimetric detection-based analysis method, detection can be finished in a short time, and qualitative detection and quantitative determination results can be obtained; and the aptamer-modified nanogold colorimetric detection-based analysis method is high in specificity and stability, wide in application range, and low in cost, and is convenient for popularization and routine application.

The invention discloses a two-dimensional microfluidic paper chip and a preparation method thereof. The two-dimensional microfluidic paper chip, from top to bottom, sequentially comprises a hydrophobic transparent membrane, a hydrophilic paper channel and a hydrophobic substrate, wherein the hydrophilic paper channel and the hydrophobic substrate are detachably connected by a waterproof double-faced adhesive tape, the surface of the hydrophobic transparent membrane is provided with a to-be-tested liquid inlet corresponding to the hydrophilic paper channel, and the preparation method of the paper chip is also very simple and quick. The two-dimensional microfluidic paper chip disclosed by the invention adopts a simple manufacturing method and is easy to carry, and can be used for rapid field test.

The invention discloses a method for quickly detecting heavy metal in water quality based on an ultra-infiltration microchip and belongs to the fields of material preparation and chemical examinationand analysis. The method comprises the following steps: firstly, reacting hydrophobic silica particles with fluorosilane in an ethanol solution to obtain superhydrophobic silica suspension; secondly,coating one side of a double-sided adhesive tape with the suspension to obtain superhydrophobic surface; finally, carrying out plasma etching in a designated area to obtain superhydrophilic microporesand an ultra-infiltration chip; pasting the ultra-infiltration chip on a specific substrate instrument by using the stickiness of the adhesive tape. By use of the ultra-infiltration chip, a color-producing reagent can be concentrated and enriched on the superhydrophilic micropores; the prepared ultra-infiltration chip can fix a detected solution on superhydrophilic sites by simple dipping operation, and colorimetric detection is realized by using a detection reagent on the hydrophilic sites. According to the method, the problems of high cost of a traditional apparatus analysis method and troublesome operation of a chemical analysis method are solved; the method is suitable for quick field detection of an aqueous solution.

The invention provides a three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol and a preparation method thereof, and belongs to the technical field of immunological detection. The preparation method comprises two parts, namely, the preparation of a monoclonal antibody and the preparation of a colloidal gold chromatographic test strip, wherein the monoclonal antibody can be used for recognizing thiamphenicol, chloramphenicol and florfenicol at the same time and is high in sensitivity; the colloidal gold chromatographic test strip comprises a polyvinyl chloride backing; a sample pad is arranged at the front end of the polyvinyl chloride backing and is connected with the front end of a nitrocellulose membrane; the rear end of the nitrocellulose membrane is connected with a water absorbing pad; the monoclonal antibody marked by colloidal gold is used as a combining pad; the nitrocellulose membrane is sequentially wrapped with a chloramphenicol sodium succinate-BSA (Bovine Serum Albumin) antigen test ray T and a goat-anti-mouse IgG control ray C. The three-in-one colloidal gold chromatographic test strip for detecting thiamphenicol, chloramphenicol and florfenicol is fast to detect, and the detection needs only 3 to 5 minutes; the test strip is convenient to carry, and suitable for detection on site; the operation is simple and convenient and does not need a professional technical person.

A method for detecting diethylstilbestrol belongs to the field of analytical chemistry, wherein the method includes the followings: pre-treating the sample to be detected, preparing the sample solution and then carrying out the following treatments: adding sample solution at one end point of the paper chromatography strip; placing the paper chromatography strip in the normal hexane-ethanol developing solvent; taking out the paper chromatography strip when the front end of the developing solvent is adjacent to the front end of the paper chromatography strip; air-drying, wherein the normal hexane-ethanol developing solvent by volume includes: 1 to 20 parts of normal hexane, 0.1 to 5 parts of ethanol; spraying the potassium ferricyanide-ferric trichloride-H2O2 on the chromatography strip; color displaying, wherein the potassium ferricyanide-ferric trichloride-H2O2 is as follows: mixing the 0.01 to 5 mol / L aqueous solution of the potassium ferricyanide and the 0.01 to 5 mol / L aqueous solution of ferric trichloride evenly; then adding 0.001% to 5% of the H2O2 by volume 1 to 1; and the volume of the H2O2 is 0 to 10% of the total volume of the solution. The invention has the advantages of simple method and device, fast detecting speed and low detecting cost, and can be used for fastly detecting the DES in food on the spot.

The invention provides a real-time monitoring system for a bottom surface of a bridge. The real-time monitoring system comprises a rail mechanism which is arranged at the bottom surface of the bridge, and a detection mechanism which is arranged on the rail mechanism and is used for detecting the actual condition of the bottom surface of the bridge; a main rail is arranged on the bottom surface of the bridge and a secondary rail is arranged at the lower part of a bridge opening and the detection mechanism arranged on the main rail is used for bearing a secondary detection mechanism; the arranged main rail is higher than the vertical height of the secondary rail; the detection mechanism moves on the main rail and the secondary detection mechanism is laid on the secondary rail along the movement direction of the main rail, so that a monitoring test is carried out in all directions and a phenomenon that a detection period is long is avoided; and acquired data information is returned back to a central control room and is subjected to rapid comparison and analysis, so that the problems of the bottom of the bridge are rapidly compared.

The invention discloses a Newcastle disease novel visualized room temperature recombinase ploymerase amplication (RT-RPA) nucleic acid test strip detection kit and applications. The kit contains the nucleotide sequences of primers and a probe which are shown in SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7, and a nucleic acid detection test strip. The application method of the kit includes the following steps: configuring an RT-RPA reaction system, and performing a normal temperature and constant temperature reaction; and directly performing interpretation on a product obtained by the constant temperature reaction after the product is detected by the nucleic acid detection test strip, a positive result appearing two red strips, one being located in a detection area, and the other one being locatedin a quality control area, and a negative result only appearing one red strip in the quality control area, and no red strips appears in the detection area. The method for rapidly detecting Newcastledisease virus can be established by adopting an RPA-nucleic acid test strip technology; and the kit and the method are simple in operation, high in specificity and safe and pollution-free, and are easy to observe reaction results and suitable for clinical site detection.

The invention discloses a direct-spread RPA (recombinase polymerase amplification) on-site visibility detection method which comprises the following steps: 1) roughly extracting genomic DNA (deoxyribonucleic acid) of a to-be-tested sample; 2) designing an RPA specific primer for a target gene; 3) adding the RPA specific primer into an RPA reaction system, and performing an PRA amplification reaction, wherein the concentrations of components in the RPA reaction system are as follows: the concentration of an outer primer F is 0.12-0.48 mu M, the concentration of an inner primer R is 0.12-0.48 muM, the concentration of dNTPs is 1.4-1.8mM, the concentration of MgOAc is 8.4-14mM, the concentration of template DNA is 1-2 mu L; the reaction procedure is carried out at 32-42 DEG C for 5-10 minutes; 4) identifying amplification results, namely putting an SYBR Green I dye into an amplification product, directly observing color change with day light or sunshine, and judging that samples of greenare positive and samples of orange are negative. The method can be applied to RPA detection on all genes from animals, plants and microorganisms, and rapid, simple, convenient and visible identification on RPA amplification products can be achieved.

The invention discloses a test strip kit for detecting nucleic acid of classical swine fever virus through room-temperature recombinase polymerase amplification (RT-RPA) and application thereof. The kit comprises primer pairs with nucleotide sequence as shown in the claim 1 and a nucleic acid test strip. An application method of the kit comprises the following steps: firstly a RT-RPA reaction system is configured for normal-temperature constant-temperature reaction; after detected with the nucleic acid test strip, a product obtained after the constant-temperature reaction directly undergoes interpretation: a positive result is two red strips with one strip positioned in a detection area and the other strip positioned in a quality control area; a negative result is a red strip in the quality control area with no red strip in the detection area. The RPA-nucleic acid test strip technology is adopted for the first time to establish a method for rapid detection of CSFV. The kit of the invention is simple to operate and has high specificity. Reaction results are easy to observe. The kit is safe and pollution-free, and is very suitable for clinic field test.

The invention discloses a method for rapid and visual paper sensing detection of heavy metal silver ions, and belongs to the technical field of heavy metal detection. The method specifically comprisesthe following steps of synthesis of nano gold, synthesis of tetra-(4-pyridyl) zinc protoporphyrin self-assembly body; preparation of a nano gold and nano porphyrin composite paper sensing device substrate, manufacturing of a silver ion standard colourimetric card, and comparison of the concentration, detected by the colourimetric card, of the silver ions in a to-be-tested sample. The method for detecting the silver ions based on the nano gold and nano porphyrin composite paper sensing substrate has the characteristics of easy preparation, rapid field detection, low cost, high response speed,high sensitivity and high selectivity, and the nano gold and nano porphyrin composite paper sensing substrate has a specific reaction to the silver ions, and can be used for detection of the silver ions in complex substrate water samples.

The invention discloses a rapid and accurate detection method for Fusarium oxysporum based on a RPA (recombinase polymerase amplification)-lateral flow chromatography test strip technology. The methodcomprises the steps as follows: 1) extracting DNA of a to-be-detected sample; 2) performing RPA with DNA as a template; 3) detecting an amplification product by a lateral flow chromatography test strip; when two brown bands appear on the test strip, one is located in the quality control area and the other is located in the detection area, the result is positive and indicates that the sample contains the Fusarium oxysporum; when only a brown band appears in the control area of the test strip and no band is located in the detection area, the result is negative and indicates that the sample doesnot contain the Fusarium oxysporum. The primer probe group has good RPA effect and strong band specificity and has no cross reaction with other bacteria, positive reaction occurs in the detection area, detection sensitivity is increased, a new technical platform is provided for detection of the Fusarium oxysporum, pathogens are detected at the early stage of disease infection, and the Fusarium oxysporum in nursery grounds or diseased plants can be detected.

The invention relates to a molecular imprinting photonic crystal detecting card and application thereof. Molecular imprinting pre-polymerization liquid is poured into a photonic crystal template; after polymerization, template molecules are washed away to obtain the molecular imprinting photonic crystal detecting card. The molecular imprinting photonic crystal pesticide residue fast detecting cardrelated by the invention has specific selectivity on target pesticide molecules; the interference of other pesticide molecules in complicated test environments can be avoided; the surface structure color can respond the outside stimulation; the naked eye visibility is realized; through contrast with a standard color card, the content of methomyl in an object to be tested can be fast obtained. Theprepared molecular imprinting photonic crystal detecting card provides an accurate, sensitive and fast novel method for detecting the methomyl pesticide residue; the reference is also provided for the development of other kinds of pesticide residue detecting technologies.

The invention discloses a primer and a probe for detecting BPIV3 (Bovine parainfluenza virus type 3) aerosol through a RPA (Recombinase Ploymerase Amplification)-lateral flow assay, a kit and a detection method. The kit contains primer probe liquid formed by a RPA primer and a probe for detecting the BPIV3, wherein the forward primer sequence is shown as SEQ ID No.1; the reverse primer sequence isshown as SEQ ID No.2; the probe sequence is shown as SEQ ID No.3. The used primer probe group has a good RPA effect; the strip specificity is high; no cross reaction with other viruses exists; strongpositive reaction exists in a detection region; the detection sensitivity is enhanced. The RPA-lateral flow assay technology is used for building a BPIV3 fast detection method for the first time; through specificity and sensitivity evaluation, the method can be used for clinical field detection; a flexible and reliable novel method is provided for BPIV3 field detection.

The invention relates to a method of ultrafast detection of antibiotic substances by combined voltage driven solid phase microextraction-raman spectroscopy. The method comprises the following steps: taking a nano golden particle wrapped porous silver wire as a working electrode, a saturated calomel electrode as a reference electrode and a platinum electrode as a counter electrode to form an electrode system, putting the electrode system in a water sample to be detected, applying -0.05 to -0.2V voltage to the electrodes, quickly enriching the antibiotic substances in the water sample onto the working electrode due to voltage driving to achieve in-situ solid phase microextraction, and performing exciting irradiation on the surface of the working electrode through a raman spectrometer for raman spectroscopy. The method increases a diffusion rate of the substances to be detected in the water sample; the nano golden particle wrapped porous silver wire is specific to the antibiotic substances, so that the antibiotic substances are enriched onto the working electrode directionally selectively; the enrichment detection speed is greatly increased; and actual applications in quick field detection and water sample pollutant surveillance are achieved.

The invention discloses a method for improving a Sudan red I detection kit and belongs to the field of analytic chemistry. According to the method, a sample is pretreated to selectively enrich Sudan red I by a magnetic solid-phase extraction mode and then is detected by the Sudan red I detection kit. The method comprises the following steps of accurately weighing the sample, putting the sample into a container, adding an extraction solvent, oscillating and standing the container, and taking supernate as upper sample liquid; adding the upper sample liquid into a container holding a magnetic solid-phase extraction material, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding normal hexane, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding acetone, oscillating the container, dropwise adding the supernate on a sample application plate, and drying the sample application plate in a blowing manner; and executing other operations according to an operating manual of the detection kit. According to the method disclosed by the invention, the Sudan red I in the sample can be effectively and quickly enriched through magnetic solid-phase extraction, so that the detection limit of the kit is reduced by 3 times, and the phenomena of false positive and missing detection are avoided to an extremely large extent. The method has the advantages of simplicity, quickness, easiness in operation and the like, and is suitable for field test, and solvents can be saved.

The invention relates to the field of nano catalysis, environment protection and analytical chemistry, in particular to a test strip for rapidly detecting pesticides. Ferroferric oxide nanoparticles with pseudo peroxidase activity are in-situ generated on the test strip, and acetylcholin esterase and choline oxidase are modified on the test strip; when organophosphorus pesticides exist, the activity of the acetylcholin esterase is irreversibly inhibited to ensure that the amount of choline generated by acetyl choline hydrolysis is reduced, so that the hydrogen peroxide generated through cascade reaction is decreased and the developing of chromogenic substrates becomes light. Through a chromatography phenomenon of test paper, interferences of other substances in samples can be eliminated. Though mobile phone photographing and mobile phone software analysis, convenient quantitative detection for acetyl choline can be realized. The test strip can be used for the fields of pesticide residual analysis, biological medicine, environment monitor and the like.

The invention provides a kit for detecting pathogenic bacteria and application of the kit, relating to the technical field of detection of a biology immune method. The kit comprises the following reagents: gold nanoparticles, a pathogenic bacterium resisting antibody, tryptophanase, and nano magnetic beads marked by the pathogenic bacteria resisting antibody. The invention also provides application of the kit in detection of the pathogenic bacteria and a detection method. The kit for detecting the pathogenic bacteria is capable of simply, quickly and sensitively carrying out in-situ detection of the pathogenic bacteria.

The invention provides an electrochemical transducer, and a preparation method and application for detecting pathogenic bacteria, and relates to the field of pathogenic bacteria detection. The electrochemical transducer comprises a working electrode, wherein the working electrode is a wave carbon electrode with the surface modified with oligonucleotide probe 2. The electrochemical transducer further comprises a working solution 1 and a working solution 2, wherein the working solution 1 is a nanometer magnetic bead dispersion liquid marked with a pathogenic bacteria antibody; the working solution 2 is a gold nanometer particle dispersion liquid marked with a pathogenic bacteria antibody and oligonucleotide probe 1; the end 5 of the oligonucleotide probe 1 is modified with methylene blue, the end 3 of the oligonucleotide probe 2 is modified with amino, and the base sequences of the oligonucleotide probe 1 and the oligonucleotide probe 2 are complementary. According to the invention, the electrochemical transducer is convenient and rapid, and the detection sensitivity is significantly improved.

The invention relates to the field of biotechnology and particularly relates to a LAMP (loop-mediated isothermal amplification) primer group and a kit for detecting influenza A virus (IAV). The primergroup comprises one or more of the following 29 primer groups: primer groups for amplifying segments of the regions H1-H18 of IVA and primer groups for amplifying segments of the regions N1-N11 of IVA. The invention also provides a kit for detecting IAV. The primer group provided by the invention has high sensitivity and high specificity; the kit prepared from the primer group can be used for quickly and accurately detecting the IAV contained in a to-be-detected sample and can be applied to the typing detection and identification of the IAV type in the to-be-detected sample. According to theprimer group and kit provided by the invention, the false positive and false negative phenomena are avoided in a process of adopting artificial RNA for control detection. The primer provided by the invention has high sensitivity and specificity; the provided visual kit brings great convenience to field detection; quick and accurate detection of IAV can be realized by preparing a kit from the primer.

The invention discloses a detection test paper for Francisella tularensis antibody, a preparation method thereof, a detection method utilizing the detection test paper and a quantitative detection system, the rapid detection method for the Francisella tularensis antibody is established by utilizing the colloid gold labeling technique and double antibody sandwich immunochromatographic technology, and the established colloid gold immunochromatographic technology for detecting the Francisella tularensis antibody can rapidly, sensitively, specifically and accurately detect the Francisella tularensis antibody in a sample, realize quantitative detection and be applicable to field rapid detection.

The invention relates to the field of biotechnology, in particular to a primer set, a reagent, a kit and a detection method for detecting and / or assisting detection of GI type norovirus. A primer setfor detecting and / or assisting in the detection of a type GI norovirus is provided, comprising a single-stranded DNA molecule as shown in SEQ ID NO. 1 and a single-stranded DNA molecule as shown in SEQ ID NO. 2. The experiment proves that the primer set of the invention has good specificity, high sensitivity, short detection time, no special instrument is needed, the experiment time cost, the simplicity of operation and the detection cost are obviously reduced. The reagent or kit containing the primer set can be used for specific detection and identification of Norovirus type GI, and the RPA detection method of Norovirus type GI based on the primer set is sensitive, accurate, simple and rapid, which can realize the screening and rapid diagnosis of Norovirus type GI.

The invention relates to the field of paper core sensing, in particular to a method of detecting silver ions and serine based on a mercaptosuccinic acid-modified CdTe quantum dot paper core sheet. Themethod herein comprises the steps of (1) preparing mercaptosuccinic acid-modified CdTe quantum dots; (2) preparing the mercaptosuccinic acid-modified CdTe quantum dot paper core sheet; (3) making a silver ion standard colorimetric card; (4) detecting silver ions of the sample. Compared with existing silver ion and serine detection methods, the method herein has the advantages of good preparationsimplicity, high field detection speed, low cost, high response speed, good sensitivity and high selectivity; the paper core sheet is specifically reactive to silver ions and serine and is suitable for detecting silver ions or serine in complex matrix water samples.

The invention relates to a piano keyboard vibration playing instrument, which comprises a controller, a piano key striking device and a piano key reset sensor, wherein the piano key striking device and the piano key reset sensor are respectively connected with the controller through a first data wire and a second data wire, the piano key reset sensor detects the position of piano keys and transmits the detection signal to the controller through the second data wire, and the controller transmits a working signal for the piano key striking device to strike the piano keys through the first data wire according to the detection signal transmitted by the piano key reset sensor. The piano keyboard vibration playing instrument can simulate the force and frequency played by people. By adopting the piano keyboard vibration playing instrument to strike the piano keyboard for a given times and / or a given frequency so as to test the variation of the tone of the piano keyboard before and after the striking, so the intonation stability of the piano from a piano factory can be rapidly judged on the spot.

The invention relates to a surface molecular imprinting SERS (Surface Enhanced Raman Scattering) sensor based on core-shell structure polydopamine coated gold nanoparticles as well as preparation andapplication of the surface molecular imprinting SERS sensor. A preparation method comprises the following steps: by taking dopamine as a functional monomer and a phthalate substance as a template molecule, carrying out self-polymerization on the surfaces of gold nanoparticles to obtain a molecularly imprinted polymer, eluting the molecularly imprinted polymer, and dropwise coating the surface of ascreen-printed electrode with the molecularly imprinted polymer to obtain the surface molecularly imprinted SERS sensor. Compared with the prior art, the invention is combined with a Raman spectrometer, by combining electrochemical enrichment and molecular imprinting technologies, high-selectivity and high-sensitivity detection of phthalate substances in a water body in a complex environment canbe realized at the same time, and the sensor has the advantages of simple preparation process, high detection speed, no need of sample pretreatment, environmental friendliness, low cost and the like,and shows a wide application prospect.

The invention provides a method for detecting chiral compound based on aptamer modified nanogold. The method is characterized by specifically comprising the following steps: step 1, preparing a gold nanoparticle solution; step 2, preparing at least one of an S-ibuprofen aptamer and an R-ibuprofen aptamer; and step 3, by taking the S-ibuprofen aptamer as a recognition probe of S-ibuprofen, mixing the gold nanoparticle solution, S-ibuprofen aptamer solution, sodium chloride solution and S-ibuprofen standard solution, making the volume constant, reacting for 30 minutes, detecting the light absorption values A650 and A520 of the detection system at 650nm and 520nm by virtue of an ultraviolet spectrophotometer, and obtaining a standard curve by taking the ibuprofen concentration as a horizontal coordinate and taking the A650 / A520 as a vertical coordinate; and detecting the S-ibuprofen concentration in a to-be-detected sample by utilizing the standard curve. The method disclosed by the invention can be finished in a short time, qualitative and quantitative detection results are obtained, the method has the advantages of high specificity, high stability, wide application range, low cost and the like, and the conventional application of chiral recognition is easily popularized.