137 results about "Florfenicol" patented technology

The invention provides an L-threonine aldolase mutant and application of the L-threonine aldolase mutant in catalytic synthesis of p-methylsulfonylphenylserine, the L-threonine aldolase mutant is derived from pseudomonas, threonine and p-methylsulfonylbenzaldehyde can be used as substrates, and the L-threonine aldolase mutant can be synthesized by using a one-step method. According to the present invention, L-threonine aldolase is adopted as a raw material, pyridoxal phosphate and reduced nicotinamide adenine dinucleotide are adopted as coenzymes, the synthesis of florfenicol intermediate product p-methylsulfonyl phenyl serine is obtained through catalysis, and compared with the wild type L-threonine aldolase, the mutant enzyme has high activity, can effectively improve the conversion efficiency, and can shorten the conversion time; the enzyme and the technical method provided by the invention have the advantages of mild reaction conditions and less pollution, and have good industrial prospects in the production of florfenicol synthesis intermediates.

The invention discloses an application of cobalt-loaded nitrogen-doped hollow carbon spheres in hydrodechlorination of electric catalytic florfenicol. According to the invention, cobalt is loaded on nitrogen-doped hollow carbon spheres to obtain cobalt-loaded nitrogen-doped hollow carbon spheres (Co / N-HCS). The cobalt-loaded nitrogen-doped hollow carbon spheres (Co / N-HCS) can efficiently remove carbon-chlorine bonds in antibiotic florfenicol molecules, and show very high florfenicol degradation rate and dechlorination rate.

The invention provides a florfenicol solution for veterinary use as well as a preparation method and application of the florfenicol solution for veterinary use. The mass percent of the florfenicol is 20%-35%, the mass percent of the inclusion agent is 6%-15%, the mass percent of the cosolvent is 5%-10%, the mass percent of the solubilizer is 10%-15%, the mass percent of the wetting agent is 0.05%-0.2%, the mass percent of the pH regulator is 0.8%-1.5%, the mass percent of the antioxidant is 0.8%-2.5%, and the mass percent of the flavoring agent is 1.0%-5.0%. The veterinary florfenicol solution disclosed by the invention is simple in preparation process, relatively good in solubility of florfenicol, clear in solution and free from precipitation after being placed for a long time. Test results show that the veterinary florfenicol solution has a remarkable treatment effect on chicken colibacillosis, and can effectively control symptoms of swine enzootic pneumonia and improve the mental state and the respiratory rate.

The invention discloses a brand-new synthesis method of florfenicol and a derivative thereof. According to the method, an enzyme chemical grade combined synthesis route is adopted, three steps are needed in total, and florfenicol and the derivative thereof are directly synthesized from p-methylsulfonyl benzaldehyde, so that the cost is reduced, the process is simplified, and industrial amplification is facilitated.

Dichloroacetonitrile is an important raw material required for the production of florfenicol, a new Class II veterinary drug in China, and has a promising market prospect. The present invention discloses a method for preparing dichloroacetonitrile, comprising the following steps: using dichloroacetaldehyde as a raw material, in the presence of different nitrogen sources and a copper catalyst, and using air as an oxidant to generate dichloroacetonitrile. The reaction liquid is filtered, extracted, and distilled to obtain a colorless liquid with a purity greater than 99%. Compared with the traditional method of preparing dichloroacetonitrile using phosphorus pentoxide, the present invention has a simple reaction, mild conditions, simple post-processing, and is more environmentally friendly, providing a new approach to the production of dichloroacetonitrile.

The invention relates to the technical field of pharmaceutical preparations, in particular to a florfenicol preparation with high water solubility and antibacterial effect and a preparation method of the florfenicol preparation. The florfenicol preparation with high water solubility and antibacterial effect is prepared from the following raw materials in parts by mass: 20 to 30 parts of florfenicol, 30 to 50 parts of beta-cyclodextrin, 30 to 50 parts of solid polyethylene glycol, 2 to 5 parts of lauryl sodium sulfate, 100 to 200 parts of dopamine aqueous solution and 1 to 5 parts of dextran sulfate aqueous solution. Wherein the concentration of the dopamine aqueous solution is 1-3 g / L, and the concentration of the dextran sulfate aqueous solution is 0.1-0.5 mol / L. The preparation method is relatively wide in application, is commonly used for granulating heat-sensitive materials and medicines which are easy to decompose when encountering water, and is convenient for subsequent re-tabletting or encapsulation; and compared with wet granulation, the method has the advantages of simple process, low energy consumption, low filler consumption and the like.

The invention discloses an iron-sulfur-carbon-based composite filler for removing florfenicol in tail water, and belongs to the technical field of water treatment materials. The composite filler is composed of charcoal, pyrite, a calcium base, a binder and zeolite in proportion, through the synergistic effect of all the components, an adsorption-reduction-degradation combined mechanism is achieved, and florfenicol in municipal tail water and aquaculture tail water is efficiently removed. The preparation method comprises the steps of ingredient mixing, roller granulation, maintenance forming and the like, is simple in process and low in cost, and can be used for recycling preparation of agricultural and mine wastes. The filler is suitable for constructed wetlands and other water treatment scenes, the removal rate of florfenicol reaches 80% or above, meanwhile, the filler has the removal effect on nitrate nitrogen and other pollutants, and wide application prospects are achieved.

The application discloses a high-water-solubility florfenicol powder and a preparation method thereof, and belongs to the technical field of veterinary drug preparations. The powder is prepared from florfenicol, a water-soluble polymer carrier, tetraethyl orthosilicate or a mixture of the tetraethyl orthosilicate and methyl triethoxysilane and a C2-C6 organic polyacid containing polyhydroxy and polycarboxyl groups as raw materials through in-situ sol-gel spray drying one-step method. In the preparation, the controllable hydrolysis and condensation of organosilane and the in-situ esterification cross-linking of the organic polyacid are used to synergistically assemble and build an organic-inorganic hybrid network skeleton, and the amorphous florfenicol is anchored in the network skeleton. The product has both instant and moisture-proof properties. The method is simple in process, and the raw and auxiliary materials are safe and easy to obtain, so the method is suitable for industrial production and solves the problem that traditional solid dispersions are easy to absorb moisture and form lumps.

The invention provides a rapid detection method for florfenicol in a meat matrix. The rapid detection method comprises the steps of sample treatment, magnetic nano material enrichment, washing and elution and the like. According to the rapid detection method for florfenicol in the meat matrix, the meat is pretreated, florfenicol in the meat matrix is fully extracted, then the florfenicol is enriched through a magnetic nano material and finally removed to serve as a detection solution, and then detection is conducted through a rapid detection card, so that the sensitivity of rapid detection is fully improved.

The invention discloses a florfenicol-polyethylene glycol conjugate and a preparation method and application thereof, and belongs to the technical field of high polymer chemistry, the florfenicol-polyethylene glycol conjugate has the following structural formula: # imgabs0 # n is 17-113, florfenicol is combined with a high-molecular polymer methoxypolyethylene glycol to form the florfenicol-polyethylene glycol conjugate, and the florfenicol-polyethylene glycol conjugate is used for preparing the florfenicol-polyethylene glycol conjugate. The problems that florfenicol is low in absorptivity, prone to being affected by metabolic enzymes and short in half-life period are solved in a nano-drug delivery mode. The florfenicol-polyethylene glycol conjugate disclosed by the invention can be used for effectively treating poultry bacterial infection, not only can be used for improving the curative effect of a medicine, but also can be used for reducing the dosage and side effects of the medicine, and a safer and more effective treatment means is provided for the poultry breeding industry.

The invention relates to a florfenicol preparation for reversing drug resistance, and every 100 kg of the florfenicol preparation is prepared from the following raw materials by weight: 30 to 50 kg of florfenicol, 5 to 10 kg of puerarin, 0.5 to 1 kg of magnesium stearate, and the balance of silicon dioxide. The florfenicol preparation is subjected to low-temperature crushing treatment, the crystal form of the medicine can be prevented from being converted, the dissolution rate of the medicine is prevented from being reduced, the florfenicol and the puerarin are reasonably proportioned according to the formula, the optimal reverse drug resistance effect is achieved, and meanwhile, the product process is particularly suitable for industrial production.

This invention relates to a chiral preparation method for florfenicol. Specifically, the method uses p-methylsulfonylbenzaldehyde and glycine ester as starting materials, and synthesizes the key chiral intermediate I through an asymmetric aldol reaction process under the action of a pyridinium salt carbonyl catalyst. Subsequently, through reduction, cyclization, fluorination, and hydrolysis ring-opening reactions, the chiral product florfenicol is obtained. Compared with existing technologies, this invention utilizes the strong catalytic ability of small-molecule pyridinium salt carbonyl catalysts, starting from inexpensive and readily available raw materials, to efficiently control the stereoselectivity of the aldol reaction process, highly selectively constructing the two chiral centers on the florfenicol structure, obtaining the desired chiral configuration, and finally synthesizing florfenicol. The preparation method of this invention is simple, rapid, and efficient.

The invention discloses an advanced oxidation catalyst for antibiotic wastewater purification, which is composed of the following components: Co3O4 and CoWO4, the molar ratio of W atoms to the total amount of Co and W atoms is 0.1-0.4, and the advanced oxidation catalyst can be used for activating peroxymonosulfate to degrade florfenicol antibiotics, and can be used for purifying the antibiotic wastewater. The florfenicol antibiotic wastewater purification catalyst still shows good catalytic degradation performance under the condition that the concentration of the catalyst is low, and can be applied to purification treatment of florfenicol antibiotic wastewater with variable concentration conditions. The preparation method of the multifunctional catalyst is simple, the cost is low, and batch amplification production and practical application of the multifunctional catalyst in florfenicol antibiotic wastewater purification are facilitated.

The application discloses a small-molecule multi-connection detection kit based on a biochip, a preparation method and a detection method thereof. The kit comprises nano-gold particle labeled antibodies, a multi-connection detection chip plate and a detection buffer. The nano-gold particle labeled antibodies comprise nano-gold particle labeled florfenicol antibodies and enrofloxacin antibodies. The multi-connection detection chip plate is coated with florfenicol antigens and enrofloxacin antigens in detection holes. The detection buffer is composed of 10-40 mM phosphate buffer and 0.05-0.5 wt% surfactant S17. The application can simultaneously detect florfenicol and enrofloxacin small molecules by using one detection buffer, thereby saving time and labor.

The utility model relates to the technical field of florfenicol production, in particular to a spiral feeder for a florfenicol fluorination procedure, which comprises a base, a spiral conveyor body is arranged at the top of the base, a moving table is arranged above the spiral conveyor body, a storage barrel is embedded at the rear end of the top of the moving table, and a feed hopper is arranged at the rear end of the top of the moving table. A barrel cover is arranged at the top of the storage barrel, the top end of the left side of the spiral conveyor body communicates with a feeding hopper, and a beating mechanism for preventing materials on the inner wall of the storage barrel from being bonded is arranged in the moving table. According to the spiral feeder for the florfenicol fluorination process, through the arranged beating mechanism, when raw materials are fed, the storage barrel can be beaten to generate vibration, so that the raw materials attached to the inner wall are shaken off, manual cleaning is not needed, and the purpose of preventing the materials from being bonded is achieved.

The present invention provides a kind of L-threonine aldolase mutant and its application in catalytic synthesis of p-methylsulfonylphenylserine, the L-threonine aldolase mutant source Pseudomonas of the present invention, can use threonine and p-methylsulfonylbenzaldehyde as substrate, with pyridoxal phosphate and reduced nicotinamide adenine dinucleotide as coenzyme, catalysis obtains the synthesis of florfenicol intermediate p-methylsulfonylphenylserine, compared to wild-type L-threonine aldolase, mutant enzyme has higher activity, can effectively improve conversion efficiency, shorten conversion time. The enzyme and technical method reaction conditions of the present invention are mild, pollution is small, and there is good industrial prospect in the production of florfenicol synthetic intermediate.

The present application relates to the technical field of antibiotic pollution remediation, and particularly relates to a method for simultaneously degrading florfenicol and inhibiting drug resistance induced by florfenicol. On the basis of traditional microbial degradation, the method regulates the community structure of a biofilm by using nano zero-valent iron, and through detection of degradation products and microbial metagenome, the method achieves effective inhibition of the singleization of the biofilm community structure and the risk of drug resistance induced by florfenicol while maintaining the microbial degradation effect, overcomes the environmental risk and public health and safety problems caused by traditional biological degradation of antibiotics, and can achieve safe and efficient removal of antibiotic pollution in water bodies.

The utility model relates to the technical field of pharmaceutical equipment, in particular to a preparation stirrer for PLGA (poly (lactic-co-glycolic acid)) nano-particles containing florfenicol medicine, which comprises a stirring barrel, a mounting rack is arranged on the stirring barrel, connecting rotating rods are arranged at the tops of two sides of the mounting rack, one end of each connecting rotating rod is fixedly connected with the outer wall of the stirring barrel, and the other end of each connecting rotating rod is fixedly connected with the stirring barrel. A driving motor is provided with an encoder and a controller, the encoder provides an accurate rotating speed feedback signal to help the controller to adjust the speed of the driving motor in real time, and under the transmission that a driving gear drives a driven gear, two groups of screw rods synchronously rotate, so that the mixing uniformity in the stirring barrel can be improved, and the stirring efficiency is improved while stirring. And through cooperation of the double-shaft motor, the rotating rod, the rotating disc and the movable rod, the barrel body can be shaken, the effect of providing power for shaking of the barrel body is achieved, so that raw materials are shaken, the up-and-down fluidity of the raw materials in the stirring barrel is accelerated, the mixing effect and efficiency are improved, and the practicability is higher.

The invention discloses an application of lanthanum hydroxide rich in oxygen vacancy in electro-catalysis florfenicol hydrogenolysis dechlorination, a preparation method of the lanthanum hydroxide rich in oxygen vacancy comprises the following steps: (1) adding alkali liquor into a lanthanum source solution, and then performing aging, solid-liquid separation and drying to obtain lanthanum hydroxide; (2) adding a glucose solution into the lanthanum hydroxide obtained in the step (1), and then drying; (3) grinding the dried product obtained in the step (2), and calcining in a nitrogen atmosphere to obtain lanthanum hydroxide rich in oxygen vacancy; loading lanthanum hydroxide rich in oxygen vacancy on a conductive substrate to prepare a working electrode; and taking the working electrode as a cathode of an electrochemical reaction device, and adding florfenicol-containing wastewater to carry out an electrocatalytic hydrogenolysis dechlorination reaction. The lanthanum hydroxide rich in oxygen vacancy can efficiently degrade the antibiotic florfenicol, and shows high florfenicol degradation rate and dechlorination rate.

The invention discloses a treatment method of a florfenicol by-product N, N-diethyl-2, 3, 3, 3-tetrafluoropropionamide, and belongs to the technical field of chemical engineering, the treatment method comprises a hydrolysis addition reaction and a cyclization reaction; the hydrolysis addition reaction comprises the following steps: mixing N, N-diethyl-2, 3, 3, 3-tetrafluoropropionamide with diethylamine and alkali liquor, and then carrying out hydrolysis reaction and addition reaction to obtain N, N, N ', N'-tetraethyl malonamide; the cyclization reaction comprises the following steps: mixing organic solvent solutions of N, N, N ', N'-tetraethyl malonamide and sodium methoxide, adding a mixed solution of formamidine and an organic solvent, carrying out reflux heat preservation reaction, evaporating materials to dryness, acidifying and filtering. According to the method, the malonamide intermediate is generated through fewer reaction steps, then the 4, 6-dihydroxypyrimidine is prepared, most of materials can be recycled, the atom economy is high, and the pollution to the environment and the post-treatment pressure are also reduced.

The application discloses a compound enrofloxacin injection, which contains 10-25g of enrofloxacin, 2-10g of sulfadimoxine sodium, 5-12g of florfenicol, 0.5-2.5g of bromhexine hydrochloride, 1-20g of glacial acetic acid, 2-5g of Tween 80, 5-15g of alpha-pyrrolidone and 5-20g of N, N-dimethylformamide per 100ml of the injection, and the rest is water for injection. The compound enrofloxacin injection has advanced and reasonable prescription design, stable and reliable preparation process, good preparation stability and small irritation, and can achieve the effect of treating all samples for respiratory system diseases of livestock and poultry.

The present application relates to aquaculture wastewater treatment technical field, specifically to a kind of surface boric acid layer modified zero-valent iron material and its preparation and application.The present application provides a kind of surface boric acid layer modified zero-valent iron material preparation method, comprising: boric acid powder is mixed with zero-valent iron powder to obtain mixture, water is added, ball milling is carried out under inert atmosphere, to obtain surface boric acid layer modified zero-valent iron.This method is based on mechanical chemistry principle, uses mechanical shearing force, impact force and friction force and other forces, boric acid, water and zero-valent iron are mechanically ball-milled, and a layer of uniform coated surface boric acid layer is generated on the surface of zero-valent iron material, to improve the removal efficiency of fluorophenylc.

The present application relates to the field of veterinary drug technology, in particular, to a kind of florfenicol metronidazole ear drops and preparation method thereof.Wherein, the florfenicol metronidazole ear drops, in parts by weight, include components: 50 150 parts of florfenicol, 10 50 parts of metronidazole, 5 20 parts of component A, wherein the component A includes benzoyl peroxide. Using the above technical solution, by adding component A containing specific ingredients to the florfenicol metronidazole ear drops, the therapeutic effect of otitis externa or otitis media with a long onset time can be improved, and efficient treatment of otitis externa or otitis media is achieved, with good application prospects.

The invention relates to the field of biological catalysis and enzyme engineering, in particular to a method for preparing a florfenicol intermediate through a double-enzyme system. The invention provides a preparation method of a florfenicol intermediate, which comprises the following steps: by taking p-methylsulfonyl benzaldehyde and threonine as raw materials and L-threonine transaldolase and alcohol dehydrogenase as catalysts, reacting in a water solution system containing a cosolvent to obtain the florfenicol intermediate (2S, 3R)-p-methylsulfonyl phenyl serine. The invention creatively provides a novel double-enzyme system of transaldolase and alcohol dehydrogenase for catalytic synthesis of (2S, 3R)-p-methylsulfonyl phenyl serine. Compared with the existing synthesis route, the production cost of the (2S, 3R)-p-methylsulfonylphenylserine is obviously reduced, the synthesis process is simplified, byproducts such as acetaldehyde are not accumulated, and the (2S, 3R)-p-methylsulfonylphenylserine better meets the requirements of current green industrial production.

The invention discloses a method for efficiently synthesizing a florfenicol key intermediate I. The intermediate I is efficiently prepared by adopting a Lewis acid / BH3-NH3 reduction catalysis system. According to the reaction, (2S, 3R)-2-amino-3-hydroxy-3-(4-(methylsulfonyl) phenyl) propionic acid is used as a substrate, BF3-Et2O is used as a catalyst, BH3-NH3 is used as a reducing agent, Et2O is used as a solvent, and an intermediate I can be directly prepared at the temperature of 15-25 DEG C. According to the method, the process of firstly carrying out esterification and then carrying out reduction in the early-stage industrialization process is avoided, so that the reaction steps are simpler and more convenient, and the enantioselectivity and diastereoselectivity are not influenced in the reaction process. Meanwhile, the catalytic reduction strategy also avoids the requirement of a traditional strong reducing agent, so that the compatibility of the reaction is more excellent, and the reaction conditions are milder.

This utility model relates to the field of pharmaceutical processing technology and discloses a spray processing device for processing florfenicol soluble powder. The device body has a heating wire inside and a top cover at its top. This spray processing device for florfenicol soluble powder generates heat within the device body through the heating wire. Then, a first drive motor drives a connecting rod installed at its output end to agitate the stirring blades, thus stirring the florfenicol soluble powder within the device body. Since the first drive motor is mounted on a connecting plate, opening the electric telescopic rod causes the connecting plate to rise and fall, effectively agitating the florfenicol soluble powder within the device body and improving the stirring effect. Simultaneously, the heating wire helps to keep the florfenicol soluble powder dry, allowing moisture to escape from the powder.

The present invention discloses the use of cobalt-loaded nitrogen-doped hollow carbon spheres in the electrocatalytic hydrogenolysis and dechlorination of florfenicol. In the present invention, cobalt is loaded onto the nitrogen-doped hollow carbon spheres to obtain cobalt-loaded nitrogen-doped hollow carbon spheres (Co / N-HCS). Cobalt-loaded nitrogen-doped hollow carbon spheres (Co / N-HCS) can efficiently remove the carbon-chlorine bond in the antibiotic florfenicol molecule, demonstrating very high florfenicol degradation and dechlorination rates.

The application belongs to the technical field of florfenicol medicine preparation, and specifically provides florfenicol nanoemulsion and a preparation method thereof. The florfenicol nanoemulsion is composed of an oil phase, an aqueous phase and a composite emulsification stabilizing solution. The composite emulsification stabilizing solution comprises a non-ionic surfactant, a co-surfactant and a polysaccharide stabilizing solution. The polysaccharide stabilizing solution is obtained after in-situ mineralization of a calcium source to generate amorphous calcium carbonate with a degradation product of kappa-carrageenan and sodium phytate as a synergistic mineralization regulator. The florfenicol nanoemulsion prepared by the application has the advantages of good stability, uniform particle size distribution and high bioavailability.

The invention discloses an adamantyl phosphine ligand with a cinchona alkali framework, a silver catalyst containing the ligand and application, the ligand has a structure as shown in a formula (I) or a tautomer, an enantiomer and a diastereoisomer of the ligand: # imgabs0 #, in the formula (I), R1 is ethyl or vinyl; and a substituent R2 is a methoxy group or a hydrogen atom. The novel catalyst composed of the adamantyl phosphine ligand with the cinchona alkali skeleton and the silver salt can efficiently catalyze the asymmetric [3 + 2] cycloaddition reaction of aromatic aldehyde and isocyano acetate, and the corresponding chiral oxazoline intermediate is obtained with high stereoselectivity and high yield; the technology can be applied to asymmetric synthesis of florfenicol intermediates and chloramphenicol intermediates, has the advantages of short synthesis route, high yield, good stereoselectivity and the like, and has good economic and social benefits.

The invention discloses a production process of a florfenicol premix with the content of 2%, and belongs to the field of preparation of florfenicol with the content of 2%. Comprising the following steps: mixing florfenicol, maltodextrin, corn starch, hydroxypropyl methyl cellulose and defatted rice bran, and granulating by using a granulator; drying and finishing the granules prepared by the granulator to obtain 2% florfenicol mixed granules with uniform diameter; totally mixing the 2% florfenicol mixed particles to obtain 2% florfenicol premix mixed particles; and packaging the 2% florfenicol premix mixed particles. The florfenicol premix has the beneficial effects that the core problems of poor uniformity, low stability, poor flowability and the like existing in a low-concentration florfenicol premix for a long time are successfully solved through a specific auxiliary material formula and key process combination of granulation, drying and finishing and total mixing; the invention provides an ideal preparation method which is stable in quality, safe and convenient to use and high in production efficiency.