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Method for detecting thiamphenicol, florfenicol and residues of metabolite florfenicol amine in egg simultaneously

A technology of florfenicol amine and florfenicol, applied in the field of florfenicol and its metabolite florfenicol amine residues, and simultaneous detection of thiamphenicol in eggs

Inactive Publication Date: 2010-06-09
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Simultaneous detection of thiamphenicol, florfenicol and florfenicol amine residues in eggs by HPLC / FLU has not been reported in China

Method used

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  • Method for detecting thiamphenicol, florfenicol and residues of metabolite florfenicol amine in egg simultaneously
  • Method for detecting thiamphenicol, florfenicol and residues of metabolite florfenicol amine in egg simultaneously
  • Method for detecting thiamphenicol, florfenicol and residues of metabolite florfenicol amine in egg simultaneously

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Embodiment Construction

[0011] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0012] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. It should be understood that these examples are only for illustration of the present invention, but not to limit the scope of the present invention in any way.

[0013] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art. The sources and trade names of the reagents used, as well as those whose components must be listed, are indicated when they appear for the first time, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.

[0014] The solution percentage concentration involved in the present invention is the mass percentage concentration unless otherw...

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Abstract

The invention discloses a method for detecting thiamphenicol, florfenicol and residues of metabolite florfenicol amine in an egg simultaneously. The method comprises the following steps: extracting, purifying and concentrating egg samples; and detecting the obtained product through a fluorescent detector at a position with 225 nm excitation waves and 285 nm transmission waves by taking solution of acetonitrile-monosodium orthophosphate as a mobile phase and by using 5 micrometer C18 column of 250 mm X 4.6 mm and at a flow speed of between 1.0 and 1.2 mL / min, wherein the solution of acetonitrile-monosodium orthophosphate has a concentration of 0.01 mol / L and contains 0.005 mol / lauryl sodium sulfate and 0,1 percent triethylamine; and the volume ratio of the acetonitrile to the solution of NaH2PO4 is 30-37: 70-65. The method for detecting the thiamphenicol, the florfenicol and the residues of metabolite florfenicol amine in the egg simultaneously has the advantages of low cost and high sensitivity.

Description

technical field [0001] The invention belongs to the field of detection of veterinary drug residues, in particular to a method for simultaneously detecting residues of thiamphenicol, florfenicol and metabolite florfenicol amine in eggs. Background technique [0002] At present, although there are many reports on the residue detection methods of thiamphenicol, florfenicol and the metabolite florfenicol amine at home and abroad, they mainly focus on the research of aquatic products, and the detection methods mainly include high-performance liquid chromatography and ultraviolet detection. (HPLC / UV), High Performance Liquid Chromatography Fluorescence Detection (HPLC / FLU), High Performance Liquid Chromatography Mass Spectrometry (HPLC-MS). The HPLC / UV method has more impurity peaks and greater interference in the sample, which requires high purification of the sample, and the sensitivity of the UV detector is low. Although the HPLC-MS method has high sensitivity, it is expensive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N1/28G01N1/40
Inventor 谢恺舟姚宜林李洋静陈书琴徐东裴燕谢星
Owner YANGZHOU UNIV
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