642results about How to "Achieving Simultaneous Detection" patented technology

The invention relates to a smoke/flame detection method based on video image analysis. The existing fire monitoring mode is limited by a plurality of geographic conditions and infrastructure constructions. The method comprises the following steps: firstly performing the motion detection on an acquired video image by using a Kalman filter; secondly analyzing the colors of smoke and flame to obtain a smoke pixel region and a flame pixel region; then using a decision machine to determine whether a corresponding region is detected, and extracting the feature of the determined region; and finally performing normalization conversion on the extracted feature so as to adapt to hidden Markov model flame detection and neural network smoke detection. The outdoor smoke and flame can be detected simultaneously by using the smoke/flame detection method based on the video image analysis so as to warn the forest fire; and the effect predicting the forest fire at an early stage is achieved, and the economic loss caused by the fire can be reduced.

The invention discloses a laser heterodyne interference linearity measuring device and a laser heterodyne interference linearity measuring method with six-degree-of-freedom detection. The laser heterodyne interference linearity measuring device comprises a laser heterodyne interference linearity and position detection part and an error detection and compensation part; a four-degree-of-freedom error detection light path consisting of three ordinary beam splitters, a polarizing beam splitter, a plane reflecting mirror, a convex lens, a position sensitive detector and two four-quadrant detectors is additionally arranged in a light path structure of the laser heterodyne interference linearity and position detection part. By utilizing a method for integrating the laser heterodyne interferometry and a laser spot detection method, the simultaneous six-degree-of-freedom detection of a deflection angle, a pitch angle, a rolling angle, horizontal linearity, vertical linearity and linearity position of a measured object can be realized, the error compensation is carried out for the vertical linearity and the vertical linearity position, the influence of rotation error of the measured object on a measurement result in the linearity measuring process can be eliminated, and the measurement precision of the laser heterodyne interference linearity and the position of the laser heterodyne interference linearity can be improved.

The invention discloses a quantum dot multicolor marking method for quantitative detection of various cardiovascular disease markers and a kit of troponin I/creatine kinase isoenzyme/myohemoglobin. The method realizes fluorescent quantitative detection by utilizing excellent fluorescent properties of quantum dots and combining a multicolour marking technology and an immunochromatographic assay on the basis of optimizing each component of a test strip. Compared with the common collaurum immunochromatographic assay, the method has the advantages of good mark stability, low nonspecificity, high sensitivity, wide linear range, small cross interference, and accuracy in quantification. The kit disclosed by the invention is used for carrying out quantification detection on the troponin I, the creatine kinase isoenzyme and the myohemoglobin simultaneously, is suitable for detection of whole blood, blood serum and plasma samples, can provide a reference for cardiovascular and cerebrovascular disease diagnosis, and is widely applied to primary hospitals and clinics.

The invention provides a preparation method of a flexible strain sensor based on conductive fiber and application thereof. The conductive fiber comprises a metal nanowire as a conductive layer, an electrospun polymer nanofiber membrane as a protective layer and an elastic yarn as an elastic carrier. In the preparation process, the surface of the elastic yarn is coated with a layer of polymer nanofiber membrane by an electrospinning technology first and then the metal nanowire is deposited on the surface structure thereof through multiple dip coating. The prepared flexible strain sensor based on the conductive fiber has the ability of quickly detecting various deformations such as stretching, bending and twisting, and the sensitivity thereof is still maintained to be 90% or above after thenumber of stretching cycles reaches 10,000; the flexible strain sensor can achieve simultaneous detection of human pulse beat, vocal cord vibration and more complex multiple sites, and has a great application potential in smart wearable devices such as virtual reality, human-machine interfaces and health monitoring.

An off-resonance photoacoustic spectrometric detection and analysis device consists of a photoacoustic spectrometric module (8). The photoacoustic spectrometric module (8) comprises a light source module (2-1), a photoacoustic signal generation module (2-2), an optical power measurement module (2-3) and a fault diagnostic analysis module (2-4). Optical signals generated by an infrared incoherence light source in the light source module (2-1) are focused by an ellipsoidal reflector to form a focused light beam, the focused light beam is modulated into light of specific frequency and wavelength by chopping blades of an optical chopper and optical filters and then transmitted to the photoacoustic signal generation module (2-2), and the fault diagnostic analysis module (2-4) calculates each gas concentration according to to-be-tested gas photoacoustic signals generated by irradiation, diagnoses faults and alarms. The off-resonance photoacoustic spectrometric detection and analysis device can be used for quantitative detection of gases such as SF6, CF4, SO2F2, SOF2, SO2, SF4 and H2O and is applicable to onsite online monitoring.

The invention discloses a beef quality multi-parameter simultaneous detection method by multichannel near-infrared spectroscopy, belonging to the rapid nondestructive detection range of beef quality. The method comprises the following steps: first, arranging a spectral detection system comprising an 8-channel fiber multiplexer, two spectrometers with different wavelength ranges and probe units ina movable control cabinet, wherein the 8-channel fiber multiplexer is respectively connected with the spectrometers and the probe units; embedding in a production line technology to carry out on-linedetection to obtain optimum spectra data, wherein the quality detection of carcass and cut meat can be simultaneously satisfied; establishing a calibration prediction model, evaluating model effects by using relative analytical errors, carrying out model analysis on a large number of sample experimental data, respectively establishing prediction models of tenderness and water content to obtain a predicted value of each index, and then plugging the obtained predicted values into a beef multi-parameter comprehensive quality evaluation model which is established based on the detected indexes to obtain a final meat grade. According to the invention, the spectral information in the visible near-infrared band is collected, the information is abundant, the detection indexes have extensibility, and the method can be suitable for the requirement of production line detection.

The invention provides a method used for simultaneous detection of three food-borne pathogenic bacteria based on multicolor upconversion fluorescence labeling. According to the method, three upconversion materials with differentiable fluorescence spectrums are used for forming multicolor upconversion fluorescent nanoprobes via respective connection with aptamers of staphylococcus aureus, vibrio parahaemolyticus, and salmonella, and complementary oligonucleotide single chains of the aptamers are connected with magnetic nanoparticles so as to form nano-composites. When bacteria to be tested are in a detection system, double chain unwinding is realized because of specific binding of the pathogenic bacteria with corresponding aptamers; it is possible to realize simultaneous quantitative determination of staphylococcus aureus, vibrio parahaemolyticus, and salmonella by monitoring upconversion fluorescence signal strength at 477nm, 550nm, and 660nm, detection linear range ranges from 50 to 1000000cfu/ml, and detection limits are 25cfu/ml, 10cfu/ml, and 15cfu/ml respectively. The method is used for detection of pathogenic bacteria, is high in sensitivity, is rapid and convenient, and can be used for detection of the three pathogenic bacteria in food such as milk and shrimp meat; and results are accurate and reliable.

The invention provides preparation and application of an alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor, and belongs to the technical fields of nano function material, clinical analysis, a bioseneor technology and electrochemistry. The characteristics that platinum nanoparticle @meso-porous silicon @ graphene nanocomposite (PtNPs@m-Si@GS) is strong in conductivity, good in stability, large in specific surface area, good in biocompatibility, strong in catalytic activity and the like are utilized in preparation; an alpha fetoprotein second antibody (anti-alpha fetoprotein (AFP)) and a carcino-embryonic antigen secondary antibody (anti-carcino-embryonicantigen (CEA)) are marked, so as to prepare the marked second antibodies Ru-PtNPs@M-Si@GS/anti-AFP and luminol-PtNPs@M-Si@GS/anti-CEA; and the sensitivity of the sensor is obviously improved. Compared with other single-channel electrode sensors, alpha fetoprotein and carcino-embryonic antigen can be simultaneously detected on a same electrode at one time; the detection efficiency is obviously improved; and the alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor has important scientific significance and application value on clinical early diagnosis of hepatic carcinoma.

The invention relates to a detection method for plant growth regulator residues in fruits. The detection method comprises the following steps: the QuEChERS method is adopted to extract and purify the plant growth regulator residues in fruits, so as to enable the adding standard recovery rate of the target compound to reach 71%-98%; the RSD ( relative standard deviation) is not higher than 6%; the sensitivity and selectivity are favorable and the recovery rate is stable; the liquid chromatograph tandem mass spectrogram detection method is adopted to simultaneously detect forchlorfenuron, ethephon, 4-sodium chlophenoxycetate and 2,4- sodium acetate-dichlorobenzene oxygen; the detection limits range from 0.5 microgram/kg to 100 microgram/kg respectively; the sensitivity requirement of national limited standard is met. The detection method has favorable sensitivity and selectivity and can serve as a method for detecting the plant growth regulator residues in fruits.

The invention relates to a Raman spectrum detection device based on a fibrescope and an implementation method of the detection device. The detection device comprises a dual-wavelength laser device, a fiber optical Raman probe, the fibrescope, a white-light cold light source, an image pick-up device, a Raman spectrometer and a display device, wherein the white-light cold light source is connected to an optical interface of the fibrescope; the image pick-up device is arranged at the upper part of the fibrescope and is used for collecting images in the fibrescope; an output end of the image pick-up device is connected to the display device and is used for displaying the images in the fibrescope; an output end of the dual-wavelength laser device is connected to an input end of the fiber optical Raman probe; and an output end of the fiber optical Raman probe is connected to the Raman spectrometer and a detector thereof. The detection device disclosed by the invention is applicable to real-time Raman spectrum detection and analysis on living bodies of intra-luminal tissues of a human body.

The invention relates to technical field of immunodetection, particularly relates to a kit for joint detection of ovarian cancer tumor markers HE4 and CA125 as well as a preparation method and application thereof. According to the kit provided by the invention, silanization treatment is performed on horse radish peroxidase, so that the possibility that the horse radish peroxidase becomes a phosphoric acid receptor in a process of catalyzing a chemiluminescent substrate to emit light by a alkaline phosphatase is blocked, the problem that the existence of horse radish peroxidase disturbs the cataluminescence of the alkaline phosphatase is solved, so that the size of the light intensity caused by the alkaline phosphatase on magnetic particles is detected by chemiluminescent substrate liquid first, the size of chromogenic absorbancy caused by the horse radish peroxidase is detected by chromogenic substrate liquid and the simultaneous detection on HE4 and CA125 of a sample is realized. Thekit provided by the invention has the advantages that the detection process is simple, the reaction time is short, the reagent dose is less, the cost is reduced, the sensitivity is high, the repeatability and stability of the reagent are good.

The invention provides a chemiluminiscence immunoassay microfluidic chip, a detector and a detection method. The chip comprises a substrate, wherein the substrate is provided with a sample injection unit, a distribution unit, a reaction unit and a waste liquor recovery unit, which are communicated in sequence through a microchannel; the sample injection unit comprises a sample injection hole; thedistribution unit comprises a first mixing groove; the reaction unit comprises a reaction groove; a curing agent layer which is used for generating immunoreaction with a to-be-detected sample is fixedin the reaction unit; and the waste liquor recovery unit comprises a first waste liquor groove. According to the chemiluminiscence immunoassay microfluidic chip, all the reagents can be previously embedded in the chip, so that the advantages that the large-scale instruments are accurate and controllable in detection result and the traditional immediate detection is rapid and simple to operation are both considered; fluid control is realized through centrifugation and sample quantification is realized through a quantification hole; and synchronous detection of multiple targets can be realized.

A gene chip for detecting viruses of leguminous plant is disclosed, several probes and nature-control collations are fixed on surface of solidoid carrier, the probes has the nucleotide sequence showed on from SEQ ID No.1 to SEQ ID No.80; the nature-control collations consists of the surface chemistry LUT, fluorescence labeling LUT, positive LUT, negative LUT, cross LUT and blank control. The advantages and innovations of the invention are following: practicability, quick-speed, and idiocrasy, and sensitivity, low activity cost, repeatability, stable and reliable results. The testing system can guarantee the repeatability and accuracy of results that is proofed by the repeatability and stability test, the system can be widely used in area of plant quarantine of the outbound and enter the country test and quarantine system, the safety supervision of plant source food, the diagnosis of plant virus in plant protecting station and the authenticate of pathogenesis in research of plant taxonomic virology. The gene chip testing call for detecting viruses of leguminous plant can be used in about 600 daily quarantine pursuits of leguminous plant source food.

The invention discloses a fluorescence chemical sensor and a method for simultaneously detecting diversified DNA (deoxyribonucleic acid) glycosylases on single-molecular levels and application of thefluorescence chemical sensor. The fluorescence chemical sensor, the method and the application have the advantages that the fluorescence chemical sensor is based on two different molecular beacons including a molecular beacon modified by 8-hydroxyl guanine and a molecular beacon modified by deoxygenated hypoxanthine, the tail end of the molecular beacon modified by the 8-hydroxyl guanine is labeled by cyanine 3 (Cy3) and quenching groups, the tail end of the molecular beacon modified by the deoxygenated hypoxanthine is labeled by cyanine 5 (Cy5) and quenching groups, the fluorescence chemicalsensor is used for detecting 8-hydroxyl guanine DNA glycosylases and N-methylpurine DNA glycosylases and is different from the traditional molecular beacons which can be severely affected by dynamicsand thermodynamics, signal restoration of the Cy3 and the Cy5 depends on molecular beacon splitting with the DNA glycosylases used as media, the DNA glycosylases can be simultaneously sensitively detected by the aid of the method without optional signal amplification, the activity of hOGG1 and hAAG can be detected by the aid of the method in an ultra-sensitive manner without optional signal amplification, the fluorescence chemical sensor can be easily, conveniently and quickly operated, and accurate and reliable test results can be obtained.

The invention provide a ultra-performance liquid chromatogram-mass spectrum detection method for 23 types of drugs and mental disease resistant drugs in blood. The method comprises the following steps: 1) sample processing: employing an organic solvent for processing a sample through protein sediment; 2) primary screening detection: employing a ultra-performance liquid chromatogram-tandem mass spectrum (UPLC-MS/MS) multi-reaction monitoring (MRM) mode for primary screening detection; and 3) employing the ultra-performance liquid chromatogram-tandem mass spectrum (UPLC-MS/MS) multi-reaction monitoring (MRM) mode for reinspection of the positive sample through the primary screening in the step 2). According to physical and chemical properties of 9 types of drugs and 14 types of mental disease resistant drugs, during reinspection, the UPLC-MS/MS detection condition which is different with the primary screening detection is employed, different sample pretreatment methods are employed, so that the detection method can satisfy the rapid, sensitive and accurate requirements of primary screening detection, and misjudgement of false positive results can be reduced.

The invention relates to a method for quickly detecting the performance of an HPLC carrier module and provides a rapid detection platform system. The system comprises an analog concentrator, a to-be-detected analog meter tool, an attenuator, a coupler, a serial server, a PC upper computer and an MDS system. Module information is read by dynamically and automatically networking an analog concentrator and a detected HPLC carrier module. The communication performance of the HPLC module can be verified under the attenuation condition of 60dB and the condition that the working voltage of the modulechanges, and a chip ID verification test, a module communication performance test, a power consumption detection test, a power supply adaptability test and the like required in state grid specifications can be quickly completed. According to the invention, the analog concentrator is used as a copying control main device to realize automatic and rapid detection of the performance of the to-be-detected communication module. Simultaneous detection of a plurality of HPLC carrier modules to be detected is realized through a concurrent meter reading mode; and a to-be-tested analog meter tool is arranged and is quickly bound with the to-be-tested HPLC carrier module, so that the service process node time is shortened, and the networking efficiency is improved.

The invention discloses an optical excitation and detection system of a fluorescent quantitative PCR detector, and belongs to a field of biological and medical science detection. The system provided by the invention comprises an optical excitation unit and an optical detection unit, wherein the optical excitation unit comprises several light sources, an excitation light optical filter and a holophote; the optical detection unit comprises several light splitting pieces, optical filters, imaging sensors and detection circuits. Light emitted from the light sources is formed into excitation light of a specific wavelength by the excitation light optical filter, the excitation light is reflected by the holophote and enters into a PCR test tube, fluorescent substances in an excitation light irradiation agent emit light, a part of the light enters into the optical detection unit from a hole in the side surface of a reaction tank, and finally the light is split and filtered by the optical detection unit and is imaged on the imaging sensors.

The invention discloses a distribution type optical fiber vibration detection method and system based on wavelength division multiplexing. A Sagnac sensing system is formed by a wide spectrum light source, a first coupler, a delay optical fiber, a second coupler, a first wavelength division multiplexer, a sensor fiber, a second wavelength division multiplexer, a Faraday rotator, a first detector and a first collection card to detect the frequency of a vibration signal; a phi-OTDR sensing system is formed by a narrow linewidth light source, a modulator, a first amplifier, a circulator, a second amplifier, a filter, a second detector, a second collection card, a second data processor, the first wavelength division multiplexer and the second wavelength division multiplexer to detect the generating position of the vibration signal. Therefore, simultaneous detection of the position and frequency of the vibration signal can be achieved.

The invention relates to a detection method for detecting foreign matters in bottled liquid based on a reflected light path design and a detection device adopting the method. According to the method, a mechanical hand is used for clamping a detection bottle containing liquid from the upper part of the detection bottle to perform bottom light detection and backlight detection; a bottom light source is used for illuminating the detection bottle from the downside of the detection bottle to the upside; a first camera is used for shooting a transmittance image of front surface bottom light reflected by a first prism; a second camera is used for shooting a transmittance image of back surface bottom light which is reflected by a side back reflecting mirror and then reflected by a second prism; a backlight source is used for illuminating the detection bottle from the back surface of the detection bottle; a third camera is used for shooting a transmittance image of backlight reflected by a third prism; the images are analyzed to determine whether foreign matters exist in the liquid in the bottle; and the detection device consists of the light sources, the prisms, the reflecting mirrors, the cameras and matching devices. The detection device disclosed by the invention is small in volume and high in detection efficiency, and can be mainly used for detecting foreign matters in various bottled liquids especially medical bottled liquids including infusion bottles and the like.

The invention provides a method for simultaneously detecting two mycotoxins through a magnetic control electrochemical adapter sensor. The method comprises the following steps: preparing an aqueous dispersion of Fe3O4 microballoons coated with gold nanoparticles; preparing a water-soluble cadmium telluride quantum dot solution and a water-soluble lead sulfide quantum dot solution; preparing a monodisperse silanization SiO2 nanoparticle aqueous dispersion; preparing the aqueous dispersion of SiO2 nano-balloons coated with cadmium telluride quantum dots and the aqueous dispersion of SiO2 nano balloons coated with lead sulfide quantum dots; preparing MB-aptamer1 and MB-aptamer2 capturing probes; coupling the complementary chain of the adapter 1 with SiO2@PbS, thereby acquiring cDNA1-SiO2@PbS; coupling the complementary chain of the adapter 2 with SiO2@CdTe, thereby acquiring cDNA1-SiO2@CdTe nanometer biological probe; preparing MB-aptamer1/DNA1-SiO2@PbS and MB-aptamer2/DNA2-SiO2@CdTe nanometer biological compound material; constructing the sensor and detecting the sample. The screen-printed electrode adopted in the method provided by the invention is simple in structure; the processing method is simple and convenient; the cost is low; the method has the characteristics of operation convenience of detection method, high detection sensitivity, and the like.

The invention provides a test kit for detecting nucleic acid of respiratory tract pathogens. The kit comprises a CRISPR-Cas detection system: crRNA, a primer pair, Cas protein and a nucleic acid probethat are described in the invention. The invention further provides a detection method of the nucleic acid of the respiratory tract pathogens, a combination of the crRNA and the primer pair and application of the combination. The test kit and the detection method disclosed by the invention can be used for directly observing results by naked eyes without depending on a large instrument, and detection can be realized under a mild condition, so that the detection is more convenient; the test kit and the detection method provided by the invention can be used for efficiently and rapidly detecting/diagnosing the respiratory tract pathogens (such as flu A, flu B and COVID-19), have relatively high specificity and sensitivity, and can be used for detecting and screening the respiratory tract pathogens, for example, rapidly distinguishing various respiratory tract pathogens including the flu A, the flu B and the COVID-19.

The invention discloses a hydrotalcite-like nanosheet@ZIF-67 composite material modified electrode, a preparation method thereof and application thereof to simultaneous detection of naphthol isomer. The preparation method comprises the following steps: growing ZIF-67 in situ on a hydrotalcite-like nanosheet stripped from formamide to prepare a cobalt-aluminum hydrotalcite nanosheet@ZIF-67 compound; and preparing a corresponding modified glassy carbon electrode from the compound. The obtained modified electrode plays a synergistic role between the hydrotalcite-like nanosheet and the ZIF-67, andrealizes high-sensitivity simultaneous detection of the naphthol isomer, wherein the linear range of alpha-naphthol detection is 3x10<->7 to 1.5x10<-4>mol/L, and the detection limit is 62nmol/L; andthe linear range of beta-naphthol detection is 3x10<->7 to 1.5x10<-4>mol/L, and the detection limit is 94 nmol/L. The modified electrode has the advantages of simple preparation method, easy control of conditions, wide linear range of detection, low detection limit, and high stability and high sensitivity of the detection method.

The invention discloses a multi-parameter electrochemical immunosensor based on an electrode array and a preparation method thereof, relating to the biosensor technology and the bioelectronics technology. The sensor is integrated with the electrode array, a counter electrode, an electrochemical reference electrode, a lead and an interface on a substrate. According to the sensor, the micro electro mechanical system (MEMS) technology is adopted to process and prepare the basic electrode array, silver and silver chloride (Ag/AgCl) are used for modifying the counter electrode and the electrochemical reference electrode, and electric media bodies, porous shielding films, nano materials, antibodies and the like are fixed on a detection electrode to form a multi-parameter detection system. The sensor has the characteristics of good stability and convenience for use, and can simultaneously detect a plurality of lung cancer biomarkers, so as to facilitate clinical application.

The invention discloses a reduced graphene oxide @ZIF-8 composite-film-modified electrode, a preparing method thereof and application of the electrode in simultaneously detecting naphthol isomers. According to the preparing method, a 2-methylimidazole solution is added into a reaction tube containing a graphene oxide agar gel matrix, a natural diffusion and penetration method is adopted for preparing a graphene oxide @ZIF-8 nano-compound, then a sample is divided into three layers according to the diffusion depth, afterwards, thermal reduction is conducted to prepare a reduced graphene oxide @ZIF-8 nano-compound, and then the corresponding modified electrode is prepared. The obtained modified electrode has the advantages of large effective area, many active sites, good dispersity and the like. Reduced graphene oxide and ZIF-8 overcome mutual defects, the synergistic effects of the reduced graphene oxide and ZIF-8 in the aspect of improving direct electrochemistry and electro-catalyticperformance are achieved, and the conductivity and catalytic performance of the modified electrode are improved. The obtained modified electrode simultaneously detects the high sensitivity of the naphthol isomers and has the advantages of being low in detection limit, wide in detection range, high in response speed and the like.

The invention discloses an optical frequency domain reflectometer with optical wave frequency shift modulation, which comprises a narrow linewidth scanning laser, a first optical fiber coupler, an optical circulator, a to-be-measured optical fiber, an optical wave frequency shifter, a second optical fiber coupler, a first polarization beam splitter, a second polarization beam splitter, a first balanced photodetector, a second balanced photodetector, a third optical fiber coupler, a first Faraday rotating mirror, a second Faraday rotating mirror, a reference optical fiber interferometer, a photodetector and a signal acquisition and processing unit. Through frequency shift modulation on measurement light and reference light, interference signals produced by superposition of scattering signal light of the to-be-measured optical fiber which is symmetrical about zero delay and local oscillation light are separated on the spectrum. According to the optical frequency domain reflectometer with optical wave frequency shift modulation, mutual interference among the interference signals of the to-be-measured optical fiber scattering signal light which is symmetrical about zero delay can be suppressed, measurement of the scattering signal light which is symmetrical about zero delay can be realized, and the maximum optical fiber measurement length can be improved.