A microfluidic biosensing platform based on upconversion luminescence

By designing an upconversion luminescent microfluidic biosensing platform that integrates mixing, reaction, and separation steps, and utilizing aptamer-mediated nanoparticle bridging flocculation, highly sensitive and rapid detection of exogenous endocrine disrupting chemicals is achieved. This solves the problems of expensive detection equipment and cumbersome procedures in existing technologies, and achieves ultrasensitive detection results.

CN116429745BActive Publication Date: 2026-05-05JIMEI UNIV +1
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Patent Information

Application Number
CN202310589390.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-05-24
Publication Date
2026-05-05
Estimated Expiration
2043-05-24

AI Technical Summary

Technical Problem

Existing technologies are insufficient for rapid, convenient, and highly sensitive quantitative detection of exogenous endocrine disrupting chemicals (EDCs), especially in field testing where there are problems such as expensive instruments and equipment, strong background interference, and cumbersome sample pretreatment steps.

Method used

Design a microfluidic biosensing platform based on upconversion luminescence, including an upconversion luminescence biosensor and a microfluidic chip. By integrating mixing, reaction, separation and detection steps, the luminescence signal is enhanced by aptamer-mediated nanoparticle bridging flocculation. Combined with an external magnetic field, quantitative detection of EDCs is achieved.

Benefits of technology

It enables micro-sampling and rapid detection of EDCs, improves detection sensitivity and signal stability, can complete detection within 10 minutes, and can detect multiple EDCs simultaneously, achieving ultra-sensitive detection results.

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Abstract

This invention relates to a microfluidic biosensing platform based on upconversion luminescence, comprising: an upconversion luminescence biosensor for specifically identifying EDCs; and a microfluidic chip for integrating the mixing, reaction, separation, and detection of the upconversion luminescence biosensor and a sample to be tested; the microfluidic chip comprising: a sample inlet for introducing the upconversion luminescence biosensor and the sample to be tested; an arc-shaped channel, the inlet of which is simultaneously connected to the sample inlet of the upconversion luminescence biosensor and the sample inlet of the sample to be tested, wherein the arc-shaped channel is used for mixing and reaction after the upconversion luminescence biosensor and the sample to be tested enter the arc-shaped channel; a separation channel connected to the outlet of the arc-shaped channel for magnetic separation of the upconversion luminescence biosensor after the reaction is completed; and a detection cell connected to the outlet of the separation channel for completing luminescence-enhanced quantitative detection of EDCs.
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