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Human blood type test kit and preparation method thereof

A technology for detecting kits and blood types, applied in the field of immunoassay, can solve problems such as hemolysis, interference of detection results, interference of detection process, etc., and achieve the effects of intuitive detection results, high sensitivity, and wide application

Inactive Publication Date: 2017-03-08
北京中检安泰诊断科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the hemagglutination test, micro-column gel test, and colloidal gold agglutination technology in the prior art are all reverse qualitative products, and the test results need to be reversely deduced to determine the results, and the operation is cumbersome, and the detection process is subject to many factors.
At the same time, flow cytometry and ELISA require professionals to operate and special equipment for interpretation
In addition, the blood samples used in the above-mentioned traditional methods cannot be hemolyzed, and hemolyzed samples will interfere with the test results and lead to misinterpretation

Method used

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  • Human blood type test kit and preparation method thereof
  • Human blood type test kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1; Human blood type detection kit

[0035] In this embodiment, the human blood type detection kit includes a test strip or a test card, and a sample diluent. figure 1 It is a schematic diagram of the structure of the detection strip in the human blood type detection kit of the embodiment of the present invention, as figure 1 As shown, the detection strip includes a reaction plate 1 , and a sample pad 2 , a gold conjugate pad 3 , a reaction membrane 4 , and a coarse fiber filter paper 5 adhered to the reaction plate 1 . In this embodiment, the gold conjugate pad 3 is coated with colloidal gold-labeled mouse anti-human A antigen monoclonal antibody, colloidal gold-labeled mouse anti-human B antigen monoclonal antibody and colloidal gold-labeled mouse anti-human D Antigen Monoclonal Antibody.

[0036] In this embodiment, the reaction membrane 4 is a nitrocellulose membrane, including a B antigen detection line 6 , an A antigen detection line 7 , a Rh detection...

Embodiment 2

[0043] Embodiment 2: the preparation method of human blood type detection kit

[0044] On the reaction membrane 4, use the mouse anti-human B antigen monoclonal antibody to coat the B antigen detection line 6, the mouse anti-human A antigen monoclonal antibody to coat the A antigen detection line 7, and the mouse anti-human D antigen monoclonal antibody to coat the Rh Detection line 8, coated with recombinant Helicobacter pylori antigen as the detection line; quality control line 9 coated with goat anti-mouse IgG polyclonal antibody; mouse anti-human B antigen monoclonal antibody labeled with colloidal gold, mouse anti-human antibody labeled with colloidal gold Antigen A monoclonal antibody and colloidal gold-labeled mouse anti-human D antigen monoclonal antibody are mixed and coated with gold-conjugated pad 3 and assembled to obtain a detection strip.

[0045] (1) Preparation of reaction membrane:

[0046] Dilute the mouse anti-human B antigen monoclonal antibody, mouse anti...

Embodiment 3

[0091] Embodiment 3: the detection method of human blood type detection kit

[0092] 1. Preparation before testing: return the kit and samples to room temperature before testing. The experimental humidity should be less than 60%, and the experimental temperature should be 18-30°C.

[0093] 2. Sample preparation: Whole blood is collected from fingertips or venously. Whole blood samples were collected and used immediately without preservation.

[0094] 3. Test strip detection method

[0095] (1) Take out the test strip from the original packaging aluminum foil bag and place it flat on the table;

[0096] (2) Take 20 μL of whole blood, add it to the sample pad 2 of the test strip, and add 100 μL of sample diluent (about 2-3 drops);

[0097] (3) Interpret the result within 3-5 minutes, and the test result will be invalid after 10 minutes.

[0098] (4) Judgment of test results:

[0099] Judgment of ABO blood group system (B antigen detection line 6, A antigen detection line 7...

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Abstract

The invention discloses a human blood type test kit which comprises a test strip. The test strip comprises a gold conjugate pad and a reaction membrane, and the gold conjugate pad is coated with a colloidal gold-labelled mouse anti-human A antigen monoclonal antibody, a colloidal gold-labelled mouse anti-human B antigen monoclonal antibody and a colloidal gold-labelled mouse anti-human D antigen monoclonal antibody; the reaction membrane comprises a B antigen test line coated with the mouse anti-human B antigen monoclonal antibody, an A antigen test line coated with the mouse anti-human A antigen monoclonal antibody, an Rh test line coated with the mouse anti-human D antigen monoclonal antibody and a quality control line coated with a goat anti-mouse IgG polyclonal antibody. The invention further provides a preparation method of the kit. The preparation method comprises the steps of reaction membrane preparation, gold conjugate pad preparation, cutting assembly and the like. The human blood type test kit can simultaneously test A, B, O and Rh blood types, is intuitive in test result, can be used for testing hemolysis samples and has the high sensitivity, specificity and stability.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a human blood type detection kit and a preparation method thereof. Background technique [0002] Blood type refers to the type of antigens on the surface of blood components (including red blood cells, white blood cells, platelets and plasma proteins). The so-called blood type usually refers to the specific antigen type on the red blood cell membrane, and is most closely related to the clinic. People are familiar with the red blood cell ABO blood group system and the Rh blood group system. [0003] Early ABO blood group and Rh blood group laboratory detection methods mainly include hemagglutination test and microcolumn gel test. The above methods have been widely used in clinical blood group identification and cross-matching in blood transfusion departments. In recent years, laboratory detection methods such as flow cytometry, enzyme-linked immunosorbent assay, and colloidal gold...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/531
CPCG01N33/80G01N33/531
Inventor 李海波周晟刘昊
Owner 北京中检安泰诊断科技有限公司
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