27 results about "Antigen test" patented technology

The invention provides monoclonal antibodies of H protein and N protein of canine distemper virus and application thereof in preparation of a fluorescent microsphere antigen detection test strip. The monoclonal antibody disclosed by the invention is characterized in that (1) the monoclonal antibody contains a heavy chain variable region CDV-N-3A11-VH and a light chain variable region CDV-N-3A11-VL; the amino acid sequence of the CDV-N-3A11-VH is as shown in the 1st to 116th sites of SEQ ID No.1 in a sequence table; the amino acid sequence of the CDV-N-3A11-VL is as shown in the 1st site to the 113th site of SEQ ID No.2 in a sequence table. (2) a heavy chain variable region CDV-H-1G5-VH and a light chain variable region CDV-H-1G5-VL are contained; the amino acid sequence of the CDV-H-1G5-VH is as shown in the first site to the 114 site of SEQ ID No.3 in a sequence table; the amino acid sequence of the CDV-H-1G5-VL is as shown in the 1st site to the 1st site and the 1st site to the 1st site of SEQ ID No.4 in a sequence table. The canine distemper virus fluorescent microsphere antigen test strip composed of the two antibodies is suitable for detecting canine distemper viruses in samples such as eye and nose swabs and virus cultures, is high in sensitivity and good in specificity, and can be used for early diagnosis of canine distemper virus infection.

The present invention discloses a method for automatically distinguishing the results of multi-directional and multi-scale new coronavirus antigen detection. The method constructs a priori structural elements, performs a priori flexible morphological operator filtering on the original image of the antigen test kit test result, calculates the a priori flexible morphological gradient, extracts the edge of the antigen test kit, extracts the test paper area to be distinguished, eliminates interference such as reflective areas, and locates potential areas in sequence to perform pre-detection of letter discrimination areas; performs discrimination letter modeling in the angle dimension to form a training set, combines samples into a tensor form to form a sample feature matrix; converts the RGB color image to the HSV color space, expands the letter image block to extract features, and constructs a feature matching operator to classify the letters C and T; performs color histogram statistics on the test paper area to be distinguished, calculates the color segmentation threshold, and detects the red discrimination mark; and splices the letter classification result and the red discrimination mark detection result to give the discrimination result of the antigen test.

A homogeneous method for detecting the nucleocapsid protein of the new coronavirus based on a nano-antibody-monoclonal antibody sandwich relates to the field of antigen detection. The present invention prepares a variety of novel coronavirus nucleocapsid protein nano-antibodies as solid-phase antibodies, nucleocapsid protein monoclonal antibodies as detection antibodies, and novel coronavirus N protein as detection targets. A group of antibody pairs with excellent detection performance are screened out by enzyme-linked immunosorbent assay, with a minimum detection line of 0.8 ng / mL. In addition, a one-step activation method couples nano-antibodies and monoclonal antibodies to the surfaces of quantum dot microspheres and magnetic nano-microspheres, respectively, and successfully prepares two probes targeting the nucleocapsid protein of the new coronavirus. It also constructs a magnetic separation immunohomogeneous method for rapid detection of the nucleocapsid protein of the new coronavirus, with a minimum detection line of 0.15 ng / mL. The present invention is simple to operate, low in cost, fast in reaction speed, can realize in situ detection, and has high efficiency in paired antibody screening. It has good application prospects in the rapid detection of novel coronaviruses.

The application discloses an antigen detection data management system based on machine learning and big data, relates to the technical field of antigen detection, and comprises a data acquisition module, a data storage module, an image analysis module and a verification module; the data acquisition module is used for acquiring image data of an antigen detection card and illumination intensity information of a shooting location; the data storage module is used for storing image data of the antigen detection card with a time stamp; the image analysis module judges an antigen detection result based on color features of the antigen detection card; and the verification module is used for acquiring color features of C lines and T lines from the start of chromatography to the completion of chromatography; the color features under different illumination conditions are connected through the chromatography degree of the C lines and the T lines, time stamp analysis is performed on image data under a condition without light interference, output data under the condition with light interference is obtained, a model under the condition with light interference is obtained through training of the output data, and the accuracy of antigen detection of a machine is improved.

Described herein are materials and methods of incorporating CLIP peptide into the peptide binding groove of HLA Class II antigens and using such HLA Class II antigens for the detection of alloantibodies.

This invention discloses a method and system for interpreting the results of multi-antigen test strips based on artificial intelligence visual recognition, belonging to the field of artificial intelligence detection technology. The method includes acquiring continuous images of radial multi-antigen test strips, performing quality assessment on the images, and selecting target analysis images; locating the center point of the test strip card based on the target analysis images, identifying the direction and number of each detection arm, establishing a polar coordinate system, and performing geometric correction on the images according to the polar coordinate system; segmenting the fan-shaped regions of each detection arm according to the polar coordinate system, locating the control line and test line in each detection arm, and obtaining a set of regions of interest for the strips; extracting the visual features of the set of regions of interest for the strips, and normalizing the test line features based on the corresponding features of the control line in the same detection arm. This invention constructs a polar coordinate correction and same-arm normalization mechanism, integrating artificial intelligence interpretation and a time-series stabilization mechanism to achieve high-precision and interpretable intelligent interpretation of radial multi-antigen test strips.

A disclosed lanthanide fluorescent antigen kit based on monkey pox virus detection comprises an antigen kit, the interior of the antigen kit is fixedly connected with a fixed placing frame, the top of the fixed placing frame is provided with two concave grooves, and each concave groove is internally provided with a plurality of antigen reagent sampling mechanisms; a clamping mechanism is arranged at a position opposite to the bottom end of the antigen reagent sampling mechanism; and an ejection mechanism is arranged at the bottom end of the clamping mechanism, the clamping mechanism comprises a clamping round frame fixedly connected with the bottom end of the interior of the concave groove, and a plurality of fixed rectangular shells are arranged at the top end of the interior of the clamping round frame. According to the scheme, a lanthanide fluorescent antigen reagent test tube for detecting the monkey pox virus is conveniently placed in a clamping round frame on the fluorescent antigen kit, and the lanthanide fluorescent antigen reagent test tube for detecting the monkey pox virus is extruded and clamped by a movable extrusion roller through telescopic matching of rectangular strips in a plurality of fixed rectangular shells.

The invention relates to a colloidal gold detection method and system of an antigen kit. The method comprises the following steps: acquiring an image of colloidal gold to be detected; obtaining the particle size range of the to-be-detected colloidal gold according to the image of the to-be-detected colloidal gold; comparing the particle size range of the to-be-detected colloidal gold with the standard particle size to obtain an initial detection result of the to-be-detected colloidal gold; if the initial detection result of the to-be-detected colloidal gold is qualified, carrying out secondary detection on the to-be-detected colloidal gold; the secondary detection comprises the following steps: respectively carrying out DLS particle size analysis, Zeta potential detection and electron microscope imaging on the to-be-detected colloidal gold to obtain particle size distribution uniformity, Zeta potential and particle size morphology regularity of the to-be-detected colloidal gold so as to obtain a detection result of the to-be-detected colloidal gold. The system and the equipment are used for executing the method. According to the invention, the artificial subjective influence in the colloidal gold quality inspection process can be reduced, and the accuracy and reliability of the colloidal gold quality inspection process are improved, so that the product quality of the antigen kit is improved.

This invention discloses a blow-type sampling viral antigen detection kit and its usage method, including a sampler, a blow nozzle, a detection working column, and a equilibration liquid dropper. Sampling is performed by blowing air, a simple sampling method that reduces the probability of sampling errors in untrained individuals. Equilibration liquid is dripped into the funnel-shaped opening of the sampler's receiving tank through the equilibration liquid dropper, continuously eluting the biological sample. The operation is simple and effective. The equilibration liquid, along with the eluted biological sample, enters a colloidal gold filter. The colloidal gold is re-dissolved, captures and labels the viral antigen in the solution. The labeled antigen then enters an antibody filter impregnated with specific antibodies, where it is captured by those antibodies, forming a conjugate of antibody, antigen, and colloidal gold microparticles. A large number of these conjugate microparticles aggregate on the filter membrane above the absorbent cotton column, resulting in color development. The sample elution step is simple, the sampling method is reliable, and the detection and colorimetric observation are easy to perform, making it convenient for the general public to use.

A method for the extraction and enrichment of Hepatitis C Virus (HCV) antigens by conjugating specific antibodies targeting Envelope 1, Envelope 2, NS3, and NS4 antigens to a temperature-responsive smart polymer (NIPAAm-Co-HIPAAm-Co-SAKIPAAm). The application also relates to a detection kit for HCV antigens, comprising the smart polymer conjugated to specific antibodies, colloidal gold nanoparticles conjugated to specific antibodies, and instructions for detecting HCV antigens in serum, plasma, or whole blood samples.

This application relates to the field of image recognition technology, and particularly to a method for determining the nasal swab collection action and a storage medium during antigen detection. The method includes the following steps: Obtain N frame video images P of a target object within T0 time during antigen detection; Traverse P, if there is a face in P i , append the face region image in P i to P 1 , and obtain M frame face region images P 1 ; Use a trained first neural network to determine whether P 1 j is a valid collection image, and obtain the number Q of A valid collection pictures during antigen detection; Traverse Q, if Q x ≥Q0, then T 1 x =T 1 x‑1 +T1; if Q x <Q0, then T 1 x =0; if T 1 x during antigen detection are all less than T, indicate non-compliance with the operation standard on the mobile terminal; otherwise, indicate compliance with the operation standard. The present invention realizes an objective determination of whether the nasal swab collection action meets the operation standard.

The invention discloses colloidal gold chromatography test paper and a kit for detecting platelet CD36 antigen, the colloidal gold chromatography test paper comprises a bottom plate, a sample pad, a gold-labeled combination pad, a nitrocellulose membrane and a water absorption pad, the gold-labeled combination pad is coated with a colloidal gold labeled mouse anti-human CD36 IgG2a monoclonal antibody and a colloidal gold labeled quality control rabbit IgG polyclonal antibody; the nitrocellulose membrane is provided with a detection line coated with a human platelet CD36 antigen capture antibody and a quality control line coated with quality control goat anti-rabbit polyclonal antibody molecules. When the kit is used for detecting the CD36 antigen, a detected sample is fully split by the platelet lysis buffer and then is added into the sample adding hole of the kit, and a detection result can be observed after waiting for 10-15 minutes. By adopting the detection mode provided by the invention, the CD36 antigen on the human platelets can be quickly, simply, conveniently, sensitively and specifically detected.

The invention belongs to the technical field of immunodetection, and discloses a folic acid sandwich method antibody mAb18 or an antigen binding fragment thereof, and a preparation method and application thereof. The folic acid sandwich antibody mAb18 or the antigen binding fragment thereof comprises a light chain variable region VL and a heavy chain variable region VH, the light chain variable region VL comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region VH comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the folic acid sandwich method antibody mAb18 is used for detecting a folic acid standard antigen, the detection sensitivity is lower than 0.2 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-20 ng / mL, and the folic acid sandwich method antibody mAb18 has important significance in the field of diagnosis and treatment of birth defects, cardiovascular diseases, tumors and neurodegenerative diseases.

This antigen measurement method comprises: a step for preparing a measurement substrate 10A which includes a substrate 110 that has a first main surface 110a and a second main surface 110b facing each other in the thickness direction, and a plurality of metal bodies 120 that is provided at intervals from each other along the first main surface 110a of the substrate 110; a step for preparing a measurement material 20 which contains metal particles 210; a step for bringing a measurement object containing an antigen 310 into contact with the measurement material 20, thereby preparing a measurement sample 30 which contains antigen-attached metal particles 410 in which the antigen 310 is bonded to the metal particles 210; a step for bringing the measurement sample 30 into contact with the measurement substrate 10A, thereby bonding the antigen-attached metal particles 410 to the measurement substrate 10A at positions that are different from the positions of the metal bodies 120 on the first main surface 110a of the substrate 110; and a step for measuring reflection absorption properties due to localized surface plasmon resonance from the first main surface 110a side of the substrate 110 of the measurement substrate 10A before and after the bonding of the antigen-attached metal particles 410 to the measurement substrate 10A, thereby measuring changes of the reflection absorption properties before and after the bonding of the antigen-attached metal particles 410 to the measurement substrate 10A.

The utility model discloses an automatic sample adding device for cell antibody detection, and particularly relates to the technical field of medical scientific research instruments, the automatic sample adding device comprises a supporting plate, a top plate is fixedly arranged on the front side of the supporting plate, a first electric push rod is fixedly arranged at the bottom of the top plate, and a U-shaped plate is fixedly arranged at the output end of the first electric push rod; a sample adding assembly is arranged at the bottom of the U-shaped plate, a sliding frame is fixedly arranged at the top of the supporting plate, a sliding block is arranged at the top of the sliding frame in a sliding mode, a glass dish is rotationally connected to the top of the sliding block, two concave blocks are arranged on one side of the glass dish, the two concave blocks are fixedly connected with the sliding block, and a moving assembly is arranged on the front side of the sliding frame. According to the utility model, the sample adding assembly is arranged, so that the functions of more accurately controlling the suction amount, the injection speed and the injection force of an antigen sample are realized, and the problem that the test result is easily influenced because the force and the dosage of sample adding each time are generally different when a cell antigen test is manually detected is solved.

The application provides an antigen detection kit automatic identification method and system based on PP-YOLOE. The method comprises the following steps: 1) collecting a large amount of antigen detection kit image data to form a known data set Z; 2) establishing a feature vector database D of negative / positive samples; 3) using a PP-YOLOE model trained on the data set Z to perform target detection, obtaining a "CT" region ROI on the antigen detection kit, and simultaneously predicting a detection result P1; 4) if P1 is located between a negative threshold and a positive threshold, a trained classification model is used to extract features of the ROI region, and then a search is performed in the already constructed feature vector database to find the feature vector with the highest similarity, that is, it is considered that the sample label is consistent with the sample label in the database, and an identification result is obtained. Compared with the prior art, the application has better generalization, more effective execution speed and higher recognition accuracy.

The invention discloses preparation of a feline distemper virus VP2 protein monoclonal antibody and application of the feline distemper virus VP2 protein monoclonal antibody in preparation of a fluorescent microsphere antigen detection test strip. The monoclonal antibody is as shown in 1) and 2), wherein the monoclonal antibody contains a heavy chain variable region FPV-VP2-2A2-VH and a light chain variable region FPV-VP2-2A2-VL; a heavy chain variable region FPV-VP2-2D9-VH and a light chain variable region FPV-VP2-2D9-VL are included, and the heavy chain variable region FPV-VP2-2D9-VH and the light chain variable region FPV-VP2 1) the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.1 in a sequence table; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2 in the sequence table. 2) the amino acid sequence of the heavy chain variable region is shown as SEQ ID No.3 in the sequence table; and the amino acid sequence of the light chain variable region is as shown in SEQ ID No.4 in the sequence table. The feline plague fluorescent microsphere antigen test strip composed of the two antibodies can be used for detecting feline plague antigens, is high in sensitivity and good in specificity, and can be used for early diagnosis of feline plague infection.

The application discloses a novel coronavirus colloidal gold antigen detection method which can be supervised, and comprises the following steps: S1, preparing a novel coronavirus colloidal gold antigen test paper card with an interference band, and storing band position information; S2, a user tests a novel coronavirus antigen and uploads personal information and test paper card test result information; and S3, feeding back an antigen detection result according to the band position information and the test paper card test result information, and storing the personal information and the antigen detection result. The application has the beneficial effect that the user cannot directly read the antigen detection result by preparing the novel coronavirus colloidal gold antigen test paper card with the interference band, and must upload the personal information and the test result, obtain the antigen detection result by searching a record module and comparing the band position information, and store the personal information and the antigen detection result to realize supervision on the antigen detection result, thereby avoiding the defect that the antigen detection result cannot be supervised in the prior art.

This invention discloses a colloidal gold immunochromatographic assay card for rapid on-site detection of paraquat and diquat, its preparation method, and its application. The assay card includes a PCV substrate, with a sample pad, a gold-labeled pad, a nitrocellulose membrane, and an absorbent pad arranged sequentially along the chromatography direction. The gold-labeled pad adsorbs paraquat colloidal gold-labeled antibody solution and diquat colloidal gold-labeled antibody solution. The nitrocellulose membrane has a T1 detection line, a T2 detection line, and a control line along the chromatography direction. The T1 detection line area is coated with paraquat complete antigen, the T2 detection line area is coated with diquat complete antigen, and the control line is coated with goat anti-mouse IgG. The assay card has the advantages of good stability, high sensitivity, and high specificity. It can simultaneously detect diquat and paraquat under different matrices. Diquat and paraquat react independently during detection, which can avoid false positives and prevent improper treatment methods from affecting the success rate of rescue.

The utility model relates to the technical field of biological detection equipment accessory design, in particular to a carcino-embryonic antigen detection kit which comprises a kit body, a side door plate is rotatably connected to the right side of the kit body, a material taking opening is formed in the left side of the kit body, a recycling opening is formed in the left side of the kit body, a middle partition plate is fixedly connected to the inner wall of the kit body, and the middle partition plate is fixedly connected to the inner wall of the kit body. A reagent box is arranged above the middle partition plate, a recycling box is arranged below the middle partition plate, and the inner wall of the right side of the reagent box is fixedly connected with two symmetrically arranged springs I. According to the reagent box, the rotating shaft can be driven to rotate by pressing the pressing rod, and a reagent can smoothly roll out along with rotation of the rotating shaft every time the pressing rod is pressed; by means of the design, the material taking process becomes quite simple and rapid, continuous multiple-time material taking is supported, the working efficiency is greatly improved, and great convenience is provided for users in laboratory detection or clinical application.

This invention relates to a lightweight cervical cancer image cell detection system based on causal attention. The system constructs sample data through various modules, using the YOLOv5 model as a foundation. By adding deformable convolutional modules guided by causal attention and employing a lightweight convolutional structure design, the system constructs and trains the model to obtain a cervical cancer image abnormal cell region detection model. This model is then used to identify abnormal cells in images from antigen detection kits. The design improves the feature extraction structure of deep networks, enhancing the model's ability to learn complex and irregular morphological features of cervical cell image regions and its generalization ability to complex backgrounds. Furthermore, by using a lightweight convolutional structure to design a finer neck network, the system reduces the number of model parameters and computational load without compromising model accuracy, thereby efficiently and accurately identifying abnormal cells in cervical cancer TCT images.

The present application relates to the field of biotechnology, in particular to a TAT enzyme-labeled antibody storage solution, and provides application of proteins, saccharides and aromatic amino acids in preparation of a thrombin-antithrombin III complex (TAT) chemiluminescence assay kit, wherein the kit reduces non-specific binding of the antibody, reduces interference of other substances in the sample on the detection result, improves the calibration curve of the chemiluminescence detection, and improves the repeatability of antigen detection.

The utility model discloses a rapid detection machine of antibody and antigen, including detection box, lifting subassembly, test tube shaking subassembly and water tank, the lifting subassembly is provided in the detection box both ends, the test tube shaking subassembly is provided on the lifting subassembly and is located in the detection box, the water tank is provided in the detection box one side, and the test tube shaking subassembly is located in the detection box one side. The water tank is connected with a flushing pipe through a water pump, and the flushing pipe extends into the detection box and is positioned below the test tube shaking assembly; the lifting assembly is used for lifting and rotating the test tube shaking assembly, the test tube shaking assembly is used for fixing and shaking test tubes in batches, and the water tank is used for washing and cleaning detected reagent tubes through a washing tube. The utility model relates to the technical field of antibody and antigen detection, and through the equipment, reagent tubes can be uniformly shaken up in batches, and the detected reagent tube solution can be quickly poured, washed and cleaned, so that the convenience of antibody and antigen detection is further improved.

A microfluidic biological sample detection chip with integrated pumping and micro-mixing functions relates to a microfluidic biological sample detection chip. The present invention aims to solve the technical problem that a long mixing time is often required to achieve a good mixing effect of the fluid in the existing microfluidic biological sample detection chip. The present invention proposes a microfluidic biological sample detection chip, which can accurately and conveniently control the flow of sample reagents, achieve efficient mixing and pumping of samples with fluorescent microspheres or magnetic beads, and further achieve efficient and high-precision detection of biological samples. Different from the water-absorbing fiber and capillary drive mode of traditional antigen detection kits and chips, the present invention utilizes a piezoelectric drive mode to achieve the functional integration of liquid pumping and micro-mixing in the same pump cavity or the same structural unit, so that biological reagents can be quantitatively pumped from the inlet to the sample detection channel, while enabling the reagents to be fully mixed with the fluorescent microspheres or magnetic beads, thereby achieving efficient integration of structure and function.

The utility model discloses a hepatitis virus antigen rapid detection card which comprises a box body, a plurality of moving plates are slidably mounted in the box body through moving grooves, a sliding rod is slidably mounted on the inner wall of each moving groove through a sliding groove, a sliding sleeve is slidably mounted on the outer wall of each sliding rod, a spring is mounted on the outer wall of each sliding rod, and the moving plates are slidably mounted in the box body through sliding grooves. The two ends of each spring are elastically connected with the inner wall of the corresponding sliding groove and the outer wall of the corresponding sliding sleeve correspondingly, and the upper end face of each sliding sleeve is fixedly connected with the outer wall of the corresponding movable plate through a connecting mechanism. Through the arrangement of the spring, the sliding block, the connecting plate and other components, when the fixing of the moving plate is released, the spring can release elastic potential energy to drive the sliding sleeve to slide on the outer wall of the sliding rod, and then the sliding sleeve drives the moving plate to move outwards in the moving groove through the connecting plate, so that the exposed area of the sampling plate can be increased; and a sample can be contacted with a chemical reagent on the sampling plate more easily and fully, so that the detection efficiency can be improved.

This invention discloses an immunochromatographic assay card, detection method, and application for broadening the quantitative detection range. The immunochromatographic assay card for broadening the quantitative detection range includes: a sample pad, a conjugation pad, a nitrocellulose membrane pad, and absorbent paper sequentially connected on a base plate; the nitrocellulose membrane pad includes a detection zone and a control zone; the detection zone is provided with a capture antibody detection line and an antigen detection line, and the control zone is provided with a control line; the conjugation pad is coated with a first label and a second label, the first label including a labeled antibody that binds to the target analyte, and the second label including a label that specifically binds to the capture reagent coated on the control line. This invention, by combining the sandwich method with a competitive method, effectively broadens the linear detection range, avoids the hook effect, and improves the accuracy and sensitivity of the detection, making it particularly suitable for quantitative detection of items with a wide concentration range.

Provided herein are methods for detecting a target antigen having a complex chemical nature, such as lipoarabinomannan (LAM), in a urine sample. In certain embodiments, to detect a target antigen having a complex chemical nature, such as LAM, the disclosure provides methods of treating the urine sample with a surfactant. The urine sample so treated, when used in an assay, such as an immunoassay that is based on the binding of the target antigen to a binding agent, provides enhanced detection of the target antigen. Also provided herein are kits for performing the methods disclosed herein.