Colloidal gold chromatography reagent strip, preparation method thereof and novel crown antigen detection kit

A technology of colloidal gold and reagent strips, which is applied in the field of new crown antigen detection kits, can solve the problems of missed detection of virus antigens such as the antibody binding ability is not outstanding, and achieve the effect of enhancing the visual effect of the naked eye, enhancing the effect of color development, and reducing the phenomenon of missed detection Effect

Active Publication Date: 2021-11-05
SHENZHEN YHLO BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Based on this, it is necessary to provide a colloidal gold chromatography reagent for detecting the new crown antigen in view of the problems that the traditional chromatography test paper needs to ...

Method used

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  • Colloidal gold chromatography reagent strip, preparation method thereof and novel crown antigen detection kit
  • Colloidal gold chromatography reagent strip, preparation method thereof and novel crown antigen detection kit
  • Colloidal gold chromatography reagent strip, preparation method thereof and novel crown antigen detection kit

Examples

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preparation example Construction

[0052] A method for preparing a colloidal gold chromatography reagent strip 10 for detecting a new crown antigen, comprising the steps of:

[0053] a) Preparation of colloidal gold particle solution: HAuCl 4 Heat to 120°C while stirring in an intelligent magnetic stirrer, add 1 / 20 to 1 / 4 volume of sodium citrate (so that the amount of sodium citrate added is 1wt%), and continue heating for 10 to 30 minutes until the color of the solution is stable It is ruby ​​red, and it can be considered that stable colloidal gold particles have been produced. After cooling to room temperature, store at 2°C to 8°C.

[0054] b) Preparation of colloidal gold nanoflower solution: add the colloidal gold particle solution prepared in step a) to 60 to 100 times the volume of purified water, heat to 60°C to 80°C, and then add HAuCl 4 (making HAuCl 4 The amount added is 1 wt%), sodium citrate (so that the added amount of sodium citrate is 1 wt%) and hydroquinone solution, stirred continuously at r...

Embodiment 1

[0065] This embodiment provides a colloidal gold chromatography reagent strip 10 for detecting the new crown antigen, and its preparation method comprises the following steps:

[0066] a) Preparation of colloidal gold particle solution: HAuCl 4 Heat to 120°C while stirring in an intelligent magnetic stirrer, add 1 / 10 volume of sodium citrate (1wt%), and continue heating for 20 minutes until the color of the solution is stable and appears as ruby, it can be considered that stable colloidal gold particles have been produced , cooled to room temperature and stored at 4°C.

[0067] b) Preparation of colloidal gold nanoflower solution: add the colloidal gold particle solution prepared in step a) to 80 times the volume of purified water, add HAuCl after heating to 60°C 4 (1wt%), sodium citrate (1wt%) and hydroquinone solution, stirred continuously at room temperature, centrifuged at a speed of 10000rpm for 20min, then removed the supernatant, collected the synthesized colloidal gol...

Embodiment 2

[0076] In this embodiment, the colloidal gold chromatography reagent strip 10 for detecting the new crown antigen prepared in Example 1 is used to quickly detect the new coronavirus antigen in the fresh sample and match the PCR result to evaluate the colloidal gold chromatography reagent strip 10 for detecting the new crown antigen sensitivity and specificity.

[0077] By testing 514 fresh nasopharyngeal swabs in 3 different locations, the results were compared with the PCR results. Add the nasopharyngeal swab to 450 μL of virus lysate, rotate and squeeze it up and down 3-10 times, take 80 μL after fully lysed and add it to the sample hole 1062 of the colloidal gold chromatography reagent strip 10 for detecting the new crown antigen, about 3 drops, after waiting for 15 minutes, observe the test results, the test results are shown in Table 1, as can be seen from Table 1, the matching results are, the sensitivity is 96.49%; the specificity is 99.25%, and the accuracy is 98.6%. ...

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Abstract

The invention discloses a colloidal gold chromatography reagent strip for detecting a new crown antigen, a preparation method thereof and a kit. The colloidal gold chromatography reagent strip comprises a bottom plate, a nitrocellulose membrane, a coupling pad, a sample pad and absorbent paper, the sample pad, the coupling pad, the nitrocellulose membrane and the absorbent paper are sequentially connected to the bottom plate in the horizontal direction, and avidin biotin amplified colloidal gold nanoflowers are coupled to the coupling pad. The nitrocellulose membrane is modified by nanocellulose, a detection line coated with a new crown antigen capture antibody and a quality control line coated with a quality control molecule capture antibody are arranged on the nitrocellulose membrane, and the detection line and the quality control line are sequentially distributed in the chromatography direction. The colloidal gold chromatography reagent strip can be used for conveniently and quickly detecting new crown antigens of nasopharynx swabs and throat swabs with high sensitivity.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a colloidal gold chromatography reagent strip for rapid and high-sensitivity detection of a new crown antigen, a preparation method and a new crown antigen detection kit. Background technique [0002] In biological detection such as the detection of the new coronavirus, existing detection technologies include fluorescent immunochromatography test paper, colloidal gold chromatography test paper, etc. Among them, the fluorescent immunochromatography test paper places the complex formed by the combination of fluorescent microspheres and antigens or antibodies in the conjugate pad. When the sample passes through the conjugate pad, the antibody or antigen in the sample reacts with the complex to generate fluorescent microspheres. The ball-antigen-antibody complex is captured by the nitrocellulose membrane and the quality control line and detection limit antibody coated on...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543G01N33/558G01N33/532
CPCG01N33/56983G01N33/54346G01N33/558G01N33/532G01N2333/165Y02A50/30
Inventor 钱纯亘黄茗张赛吴力强
Owner SHENZHEN YHLO BIOTECH
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