Preparation and application of renewable terahertz biological sample detection cell

A biological sample and detection cell technology, applied in the field of terahertz biological sample detection cell, can solve the problems of many operation steps and complex detection

Active Publication Date: 2020-01-07
张阳
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The purpose of the present invention is to provide a reproducible terahertz biological sample detection pool and ultra-sensitive quantitative detection method to solve the problem of multiple operating steps in the antigen-specific detection process , require special markers, detect complex problems

Method used

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  • Preparation and application of renewable terahertz biological sample detection cell
  • Preparation and application of renewable terahertz biological sample detection cell
  • Preparation and application of renewable terahertz biological sample detection cell

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Embodiment Construction

[0047] The following is further described in detail through specific implementation methods:

[0048] combine Figure 1-9 As shown, the preparation of a reproducible terahertz biological sample detection cell includes the following steps: (1) Substrate substrate cleaning: take the silicon wafer and cut it, put the cut silicon wafer into an ultrasonic cleaner for cleaning, After taking it out, first clean it with an organic solvent and then rinse it with deionized water. In this embodiment, the organic solvent can be selected from ethanol, acetone or methanol, preferably ethanol, and then dry the surface of the silicon wafer with high-purity nitrogen;

[0049] (2) Deep silicon etching: engrave a single channel with a length and width of 7200 μm and a depth of 220 nm in the middle of every 1.5*1.5 cm area on the silicon wafer dried in step (1);

[0050] (3) Coating: a gold film with a thickness of 120nm is plated on the surface of the channel;

[0051] (4) Coating: Photoresist...

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Abstract

The invention belongs to the technical field of biological sample detection, and particularly discloses an ultra-sensitive, unlabeled and renewable specific biological macromolecule detection method.The method comprises the following steps of (1) preparing the renewable terahertz biological sample detection cell, namely cleaning a substrate, carrying out deep silicon etching, coating, gumming, exposing, developing, carrying out post-baking, carrying out wet etching and cutting; (2) modifying a high polymer membrane strip through a polyethyleneimine-glutaraldehyde crosslinking method to prepare an antibody cross-linked detection membrane strip; (3) carrying out THz-TDs detection; (4) adding an antigen for incubation; (5) carrying out THz-TDs detection; and computing a frequency shift variation deltaf; and (6) repeating the steps (3) to (5) to detect biomolecular solutions of different concentrations. The perpetration and the application are mainly used for detecting oxidized low-density lipoprotein solutions of different concentrations, the problems of insufficient terahertz detection sensitivity and difficulty in metamaterial cleaning are solved, and the specific and unlabeled antigen detection is realized.

Description

technical field [0001] The invention belongs to the technical field of biological sample detection, and specifically discloses a reproducible terahertz biological sample detection pool and an ultrasensitive, specific and quantitative detection method. Background technique [0002] Terahertz waves are electromagnetic waves with a frequency between 0.1 and 10 THz. Based on THz, it is possible to perform label-free, non-contact and non-destructive detection of biological substances. The weak interactions between nucleic acid, protein and other biomacromolecules (such as hydrogen bonds, van der Waals forces, etc.), skeleton vibration and dipole rotation are just in the THz spectrum range, and each biomacromolecule has a specific THz spectrum fingerprint. Therefore, THz waves can detect information about the composition, structure and function of biological macromolecules that cannot be obtained in other electromagnetic bands. THz waves can reveal the structural and functional i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3586
CPCG01N21/3586G01N2001/2873
Inventor 张阳谢明真府伟灵
Owner 张阳
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