14 results about "Oxidized low density lipoprotein" patented technology

The invention discloses a 4E6 antibody-based oxidized low-density lipoprotein detection method, which comprises: obtaining a model constructed by a target 4E6 monoclonal antibody sequence, carrying out docking analysis on the interaction of antigen binding regions, designing a mutant prediction influence, selecting an optimal combination to construct a new sequence, and carrying out expression purification to obtain the oxidized low-density lipoprotein. The optimization effect is verified by measuring and combining kinetic parameters; a dendritic-linear polymer modified structure on the surface of a latex microsphere is designed and optimized based on a computational material science method, and the modified latex microsphere is synthesized and coupled with an optimized 4E6 monoclonal antibody to form a detection probe; and establishing an oxidized low-density lipoprotein standard curve through a plurality of groups of standard substance solutions, and determining a detection limit and a quantitation limit so as to calculate the sample concentration. According to the scheme, the antibody recognition capability and the signal amplification effect can be improved, and the detection sensitivity and accuracy are improved.

The invention discloses a kit for determining oxidized low-density lipoprotein based on latex enhanced immunoturbidimetry and application, and belongs to the technical field of biological detection reagents, the kit comprises a reaction buffer solution and a latex reagent, the reaction buffer solution is marked as R1, and the latex reagent is marked as R2; the reaction buffer solution comprises 3-10 g / L of a buffer solution, 5-15 g / L of inorganic salt, 10-30 g / L of a turbidity increasing agent, 0.5-2.5 mL / L of a surfactant, 1-3 mL / L of a preservative and 3-14 g / L of a stabilizer; the latex reagents are at least two latex reagents formed by coupling monoclonal antibodies and latex microspheres, and the particle size range of the latex microspheres is 200-280 nm. The latex is marked by adopting two monoclonal antibodies with relatively strong specificity, so that the specificity is relatively strong under the condition of keeping the sensitivity. According to the kit, the detection sensitivity and specificity can be improved at the same time, so that the linear range of the kit is wider, the accuracy is higher, and clinical requirements are better met.

The application discloses a kind of near-infrared afterglow nano probe and its preparation method and application, wherein the near-infrared long afterglow nano probe is near-infrared long afterglow nano probe, particle size is 100nm, and its composition is: ZnGaSnO4:Cr, Y, wherein, the ratio of Zn, Ga, Sn, Cr, Y is 1.3:1.4:0.3:0.005:0.003, the near-infrared long afterglow nano probe of the application has higher luminous intensity and excellent fluorescent performance, is a new type of luminescent material, can emit light for more than several hours under external environment, has high luminous intensity, long life and good chemical stability and other characteristics, has the advantages of low background, strong fluorescent signal, high signal-to-noise ratio and the like, label is connected with oxidized low density lipoprotein monoclonal antibody by covalent bond, and the labeled product is stable, has the characteristics of high sensitivity, high accuracy, rapid and simple detection and the like, early diagnosis, curative effect monitoring and prognosis evaluation of coronary heart disease and atherosclerosis can quickly help clinical diagnosis.

The present application relates to the technical field of biological detection, and particularly relates to a system and a method for detecting oxidized low density lipoprotein. The PLA-HCR metallization system provided by the present application constructs an "AND" logic gate based on a proximity effect, realizes direct recognition and signal amplification of oxLDL complete particles in combination with a hybridization chain reaction, and overcomes the limitation in traditional methods that only rely on indirect reflection of lipoprotein particle concentration between single components; the system uses gold-platinum bimetallic nanoparticles to endow oxLDL particles with high-efficiency exogenous peroxidase catalytic activity, and compared with traditional peroxidase, the enzyme catalytic efficiency is significantly improved, and the system has the ability to directly detect undiluted serum samples, avoiding the cumbersome pretreatment process of traditional ELISA methods. The present application has the characteristics of high specificity, wide detection range and simple operation, and has a wide application prospect in early diagnosis, curative effect monitoring and prognosis evaluation of patients with atherosclerotic cardiovascular disease.

The invention relates to the technical field of biological detection, in particular to a system for detecting oxidized low-density lipoprotein and a detection method. According to the PLA-HCR metallization system provided by the invention, an AND logic gate is constructed based on a proximity effect, and is combined with a hybridization chain reaction to realize direct recognition and signal amplification of oxLDL complete particles, so that the limitation that the concentration of lipoprotein particles is indirectly reflected only by depending on a single component in a traditional method is overcome; according to the system, high-efficiency exogenous peroxidase catalytic activity is endowed to oxLDL particles by using gold-platinum bimetallic nanoparticles, compared with traditional peroxidase, the enzyme catalytic efficiency is remarkably improved, the system has the capability of directly detecting an undiluted serum sample, and the tedious pretreatment process of a traditional ELISA method is avoided. The kit has the characteristics of high specificity, wide detection range, simplicity and convenience in operation and the like, and has wide application prospects in early diagnosis, curative effect monitoring and prognosis evaluation of atherosclerotic cardiovascular disease patients.

The invention discloses an apolipoprotein B (ApoB) antibody related to oxidized low density lipoprotein (Ox-LDL) or a functional fragment of the apolipoprotein B antibody, and an application of the apolipoprotein B antibody and the functional fragment. The anti-Ox-ApoB antibody or the functional fragment thereof disclosed by the invention comprises a heavy chain complementarity determining region and a light chain complementarity determining region, and the antibody or the functional fragment thereof has relatively good specificity and sensitivity to Ox-ApoB, and can be used for detecting Ox-ApoB and / or Ox-LDL and used for auxiliary diagnosis of atherosclerosis by taking Ox-ApoB and / or Ox-LDL as a marker.

This invention belongs to the field of bioassay technology, and specifically relates to a method and kit for detecting oxidized low-density lipoprotein (ODL). This invention provides a novel application of quaternized chitosan in the kit, specifically using quaternized chitosan of different molecular weights in the immunoturbidimetric detection of ODL. Results show that when high molecular weight quaternized chitosan (100-200 kDa) and low molecular weight quaternized chitosan (30-50 kDa) are used as components of the ODL detection kit, the different molecular weights of quaternized chitosan react with citrate to form gel particles of varying mechanical strengths. This allows for the detection of ODL content via immunoturbidimetry. Furthermore, the use of quaternized chitosan of different molecular weights significantly improves the sensitivity and linear range of the ODL detection kit.

This invention discloses an antioxidant low-density lipoprotein monoclonal antibody and its applications, belonging to the field of antibody technology. The antioxidant low-density lipoprotein monoclonal antibody of this invention possesses high specificity, high affinity, and high sensitivity. An immunoturbidimetric assay kit prepared based on the antioxidant low-density lipoprotein monoclonal antibody of this invention can quantitatively detect oxidized low-density lipoprotein in peripheral blood samples, and can have a good diagnostic effect on cardiovascular diseases caused by atherosclerosis (AS).

The invention provides application of SPPARMs in treatment of atherosclerosis, and belongs to the technical field of biological medicine. An ApoE gene knockout mouse is subjected to high-fat feeding to construct an atherosclerotic mouse model, THP-1 macrophages are exposed to oxidized low-density lipoprotein to construct a macrophage foaming model, INT131 is used for drug administration to an animal model and a cell model, and results show that INT131 can effectively relieve formation of atherosclerotic plaques, and can be used for treating atherosclerotic plaques. The content of AS mouse serum total cholesterol and low-density lipoprotein cholesterol is obviously reduced, and the content of high-density lipoprotein cholesterol is increased. In addition, INT131 can reduce intake of lipid in macrophages, promote cholesterol excretion and cholesterol metabolism, and inhibit formation of foam cells by inhibiting inflammatory response.

The invention provides a medicine for treating atherosclerosis and application thereof, and belongs to the technical field of cardiovascular disease treatment. The medicine consists of the function-enhanced mesenchymal stem cells and a medicinal carrier. The function-enhanced BMSCs are obtained by treating bone marrow mesenchymal stem cells with a composition of praeruptorin E, lobetyolin and theaflavin in a specific concentration ratio in a culture process. Experimental results show that the BMSCs prepared by the method have significantly enhanced migration ability and secretion function, and can effectively protect endothelial cells from damage induced by oxidized low-density lipoprotein. Animal model research further proves that the function-enhanced BMSCs can significantly inhibit the formation of atherosclerotic plaques and show a significant treatment effect.

Compositions and methods for treating a subject with psoriasis are provided, including an inhibitor of oxidized low density lipoprotein (LDL), malondialdehyde-modified epitope in LDL or native LDL for administration. Exemplary inhibitors of oxidized or malondialdehyde-modified LDL include orticumab, a variant antibody or peptides capable of binding oxidized or malondialdehyde-modified LDL, which results in improvement in conditions of psoriasis even in patients that are refractory to ultraviolet A / B therapy and topical steroids.

The application discloses a method and equipment for detecting oxidized low-density lipoprotein based on an immune turbidimetry method, and the method comprises the following steps: preparing a macromolecular initiator by adopting an active esterification reaction, and preparing a block graft copolymer by means of an atom transfer radical polymerization and a radical grafting reaction; the surface of different particle size latex microspheres is modified by using the graft copolymer, then the microspheres with different particle sizes are coupled with different OxLDL monoclonal antibodies respectively, so as to prepare double-particle-size latex microsphere markers; a serum sample is mixed with the double-particle-size latex microsphere markers, and an absorbance value is measured, and the concentration of oxidized low-density lipoprotein in the serum sample is calculated according to a standard curve established based on a standard sample solution. Through the scheme, the stability of the latex microsphere marker can be enhanced, and the binding efficiency of the latex microsphere marker and ox-LDL is improved.

The invention relates to the technical field related to biological medicine, in particular to a method for inhibiting cholesterol esterification of macrophages, through targeted regulation of a PPAR-gamma / LXR-alpha signal channel by means of palmatine (PAL), foam cell formation can be effectively reduced, and accordingly atherosclerosis (AS) can be improved. Oxidized low-density lipoprotein (ox-LDL) is used for inducing foaming, then 25-100 [mu] g / ml palmatine is used for intervening for 48 hours, and experiments show that the palmatine remarkably reduces the cholesterol esterification rate (from 52.17% to 41.88%, Plt; according to the present invention, with the application of the palmatine in the preparation of the medicine, the expression of cholesterol efflux genes such as ABCA1, ABCG1 and the like is up-regulated, the release of inflammatory factors IL-6 and TNF-alpha is inhibited (reduced by more than 60%), and the molecular docking proves that the combination of the palmatine and the PPAR-V is stable (the combination energy is-8.482 kcal / mol), and is superior to the traditional medicine; the invention provides a new strategy for natural, safe and multi-target intervention of AS, and has significant clinical application potential.

The invention relates to the technical field of detection kits, and provides an oxidized low-density lipoprotein colloidal gold immunochromatography detection kit. According to the kit for detecting the oxidized low-density lipoprotein, the oxidized low-density lipoprotein in serum, plasma and whole blood can be detected at the same time, treatment modes such as centrifugation and the like do not need to be carried out on a blood sample, and the detection time is greatly shortened; the kit has the advantages of high sensitivity, high repeatability, high specificity, wide linear range, low cost and the like, has the advantages of convenience in operation and detection while arrival of POCT (point-of-care testing) rapid diagnosis, and saves precious time for bedside detection, remote mountainous area and community detection and emergency treatment detection.