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Lactobacillus plantarum inducia dsm 21379 as enhancer of cellular immunity, hypocholesterolemic and Anti-oxidative agent and antimicrobial agent against clostridium difficile

a technology of clostridium difficile and lactobacillus plantarum, which is applied in the field of microorganisms and nutrition, can solve the problems of increasing mortality rates, complex ripening period, and profound imbalance of gi microorganisms, so as to prevent the cholesterol metabolism disorders and reduce the level of ldl-cholesterol

Inactive Publication Date: 2014-11-13
TERVISLIKU PIIMA BIOTEHNOLOOGIATE ARENDUSKESKUS OU BIO COMPETENCE CENT OF HEALTHY DAIRY PROD
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  • Abstract
  • Description
  • Claims
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Benefits of technology

[0036]The purpose of the present invention is to provide a strain Lactobacillus plantarum Inducia DSM 21379 for use as hypocholesterolemic agent for decreasing the level of LDL-cholesterol in blood for preventing the cholesterol metabolism disorders and consecutive cardiovascular disorders.
[0037]Another purpose of the instant invention is to provide the aforesaid strain for use as an antimicrobial agent for lowering risk of Clostridium difficile associated diarrhoeae (CDAD) by preventing germination of Clostridium difficile spores and by suppressing proliferation of Clostridium difficile vegetative cells.
[0038]The next purpose of this invention is to provide Lactobacillus plantarum Inducia DSM 21379 for use as anti-oxidative agent for enhancing anti-oxidative activity of blood by reducing the level of oxidized low density lipoprotein (ox-LDL) and by enhancing the total anti-oxidative activity (TAA) of a composition comprising said strain.
[0039]The current invention also relates to the compositions comprising L. plantarum Inducia DSM 21379 for decreasing the levels of LDL-cholesterol and ox-LDL in blood, lowering risk of CDAD and for enhancing the natural defense potential and cellular immunity of a human.
[0040]Due to the fact that L. plantarum Inducia DSM 21379 produces polyamines from ornithine and glutamate, nitric mono-oxide (NO), the composition comprising said strain possesses antioxidative activity and improves the intestinal barrier function, increases the number of immunocytes in blood and induces cytokine synthesis for enhancement of organisms' natural defense. Lactobacillus plantarum Inducia DSM 21379 is used as enhancer of the natural defense potential and cellular immunity of a subject, and simultaneously as anti-oxidative agent as well as hypocholesterolemic agent.

Problems solved by technology

Incorporation lactobacilli of human origin into a food product different from other milk-based products and having a long ripening period could be complicated.
But it is well known that the application of broad-spectrum antimicrobial preparations for treatment of infections and inflammatory complications may cause profound imbalance among GI microbiota.
C. difficile-associated diarrhea (CDAD) increases mortality rates, lengthens hospitalization and dramatically increases overall health care costs.
These effects appear to reduce the favourability of the environment for C. difficile.
Next, the possibility for intestinal barrier protection with probiotics may result in interfering with the binding of C. difficile toxins A and B to colonic epithelial cells thus stabilizing gut permeability and inhibiting development of pseudomembranes on epithelia of gut.
Furthermore, some clinical trials could not reach statistical evidence to demonstrate the effect for the prevention of Clostridium difficile associated diarrhea (CDAD) of certain probiotics.

Method used

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  • Lactobacillus plantarum inducia dsm 21379 as enhancer of cellular immunity, hypocholesterolemic and Anti-oxidative agent and antimicrobial agent against clostridium difficile
  • Lactobacillus plantarum inducia dsm 21379 as enhancer of cellular immunity, hypocholesterolemic and Anti-oxidative agent and antimicrobial agent against clostridium difficile
  • Lactobacillus plantarum inducia dsm 21379 as enhancer of cellular immunity, hypocholesterolemic and Anti-oxidative agent and antimicrobial agent against clostridium difficile

Examples

Experimental program
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Effect test

example 1

Obtaining Needed (Optimal) Viable Counts of Lactobacillus plantarum Inducia DSM 21379 in Food Product

[0061]Test with Estonia Cheese

[0062]Method. Microorganism Lactobacillus plantarum Inducia DSM 21379 was added to the cheese milk of Dairy Cooperative E-Piim, (inoculation dose 3×108 CFU / vat) and the milk was renneted (25 min). The curds were cut (25 min), heated (34° C. 15 min), dried (25 min), pressed, drained (1 h), salted in brine (12° C.; 20% NaCl; pH 4.7) 20 h, drained and dried (8 h), backed into plastic and ripened at 12° C. for at least 4 weeks.

TABLE 5Dynamics of microflora in Lactobacillus plantarum Inducia DSM 21379 comprising cheese.L. plantarum Inducia DSM Control 21379 comprising cheesecheeseSampleDay 14Day 21Day 30Day 14Day 21Day 30total counts8 × 1084 × 10810102 × 1083 × 1082 × 108Lactobacillus5 × 1082 × 1081010105—3 × 103spL. plantarum5 × 1082 × 1081010——3 × 103cocci3 × 1082 × 108109 2 × 1083 × 108108spore-forming——103 —107108microbesL. casei———105.—

I. Lactobacillus p...

example 2

Production of Polyamines in Food Product by Lactobacillus plantarum Inducia DSM 21379

[0063]Method. Cheese samples were extracted (20 ml 50% methanol solution was added to 10 g of cheese and incubated at 45° C. for 1 h, cooled to 30° C. and centrifuged) and 200 μl of upper layer was derivatized for GC analyze by modified method of Nakovich (Nakovich, L. 2003 Analysis of biogenic amines by GC / FID and GC / MS).

[0064]GC analysis were carried out by gas chromatograph HP 6890 Series GC System, with capillary colonna HP-5 19091J-413 (30 m×0.32 mm; 0.25 μm). The column temperature program 160° C. 1 min, 20° C. / min 280° C. 15 min; and detector (FID) 350° C.

[0065]The production of putrescine and tyramine was related to that: in different lots in comparison with control-cheese putrescine content increased 3-11 times and tyramine content accordingly 2-5 times.

[0066]Lactobacillus plantarum Inducia DSM 21379 belongs to the facultatively heterofermentative group of lactobacilli and therefore the con...

example 3

Enhancement of Defense Capability of Intestinal Mucosa with Lactobacillus plantarum Inducia DSM 21379 Comprising Food

[0070]In the experimental model with NIH mice 3 groups of mice consumed different cheeses during 30 days (control cheese with no additives, Lactobacillus plantarum strain Inducia DSM 21379 2×108 cfu / g comprising cheese).

[0071]Cheese was administered to mice at night (normal awake time for mice) 4.4 g / per mouse, daily ad libitum regular commercial diet. Consumed amount of cheese was 3.5-4.2 g / per night.

[0072]Mice stayed in good condition, no changes in fur and digestion was detected. Cheese administration caused increase of body weight: body weight at the beginning of the trial was 22.9-29.8 g and at the end of the trial a weight gain was 2-6.1 g. The mice were sacrificed by cervical dislocation at day 30. No translocation of lactobacilli or other bacteria of the microflora to blood or organs was detected at the autopsy.

TABLE 7Total counts of lactobatsilli in faeces, i...

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Abstract

A microorganism strain Lactobacillus plantarum Inducia DSM 21379 for use as hypocholesterolemic, antimicrobial and anti-oxidative agent and as enhancer of the natural defense potential of a subject. L. plantarum Inducia is used for reducing the level of LDL-cholesterol in blood for preventing the cholesterol metabolism disorders and cardiovascular disorders. L. plantarum Inducia enhances simultaneously the natural defense potential and cellular immunity, and as anti-oxidative agent enhances anti-oxidative activity of blood by reducing the level of oxidized low density lipoprotein (ox-LDL). The strain is also used as an antimicrobial agent for lowering risk of Clostridium difficile associated diarrhoeae (CDAD) by preventing germination of C. difficile spores and by suppressing proliferation of its vegetative cells. The compositions comprising L. plantarum Inducia decrease the levels of LDL-cholesterol and ox-LDL in blood, enhance the natural defense potential and lower risk of CDAD. The composition may further comprise xylitol.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation-in-part and claims the benefit under 35 U.S.C. §120 of U.S. application Ser. No. 12 / 992,862, filed Nov. 15, 2010, the entire contents of which are incorporated herein by reference, which is a 371 National Stage of International Application No. PCT / EE2009 / 000006, filed May 12, 2009, the entire contents of which are incorporated herein by reference, which claims priority from prior EE Application No. P200800027, filed May 13, 2008.TECHNICAL FIELD[0002]The present invention relates to the field of microbiology and nutrition referring to Lactobacillus plantarum Inducia DSM21379 strain which has various applications in the field of biotechnology, food industry and medicine.BACKGROUND ART[0003]During the past decades lactobacilli have widely been used as probiotics in functional foods. Food can be regarded as functional, if beyond adequate nutritional components it contains some natural additives (pre- or prob...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/74C12N1/20A23C9/123A61K35/747
CPCA61K35/747A23C9/1234C12N1/20A23Y2220/67A61K2035/115A23C19/054A23L33/135A23K10/18A23C19/061A23C19/062A23V2002/00A61K31/047A61P1/12A61P31/04A61P3/06C12R2001/25C12N1/205A23V2400/169A23V2200/00A23V2200/3204A23V2200/324A23V2200/326A23V2200/3262A23V2250/6422A23C9/123A61K35/74C12N1/00
Inventor MIKELSAAR, MARIKASONGISEPP, EPPHÜTT, PIRJESMIDT, IMBITRUUSALU, KAIRÄTSEP, MERLEKÕLJALG, SIIRISTSEPETOVA, JELENAKILK, KALLEZILMER, MIHKELSEPP, EPPMIKELSAAR, RAIK-HIIO
Owner TERVISLIKU PIIMA BIOTEHNOLOOGIATE ARENDUSKESKUS OU BIO COMPETENCE CENT OF HEALTHY DAIRY PROD
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