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Cordyceps militaris sporocarp heteropolysaccharide and application thereof

A technology of heteroglycans and grass seeds, which is applied in the field of heteroglycans and its preparation in the fruiting body of Cordyceps militaris, which can solve the problems of wide range of molecular weight, complex composition, structure and preparation method, etc.

Active Publication Date: 2016-05-18
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the process of obtaining polysaccharides by water extraction and alcohol precipitation is simple, the obtained polysaccharides have a wide range of molecular weight and complex components, which is not conducive to the elaboration of the structure-activity relationship of polysaccharides, nor is it conducive to product quality control and subsequent clinical transformation applications.
[0007] In recent years, researchers at home and abroad have reported the lipid-lowering and anti-atherosclerosis effects of Cordyceps militaris extract alone or in combination, but the polysaccharides in the fruiting bodies of Cordyceps militaris have anti-atherosclerosis and lipid-lowering functions. The composition and its structure and preparation method have not been reported

Method used

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  • Cordyceps militaris sporocarp heteropolysaccharide and application thereof
  • Cordyceps militaris sporocarp heteropolysaccharide and application thereof
  • Cordyceps militaris sporocarp heteropolysaccharide and application thereof

Examples

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Effect test

Embodiment 1

[0030] Example 1: Preparation of heteropolysaccharide TY258 from the fruiting body of Cordyceps militaris and its anti-atherosclerosis application

[0031] When extracting and preparing the heteropolysaccharide TY258 of the fruiting body of Cordyceps militaris of the present invention and verifying its lipid-lowering and anti-atherosclerosis effects on the apolipoprotein E knockout mouse model, the following steps were taken:

[0032] 1) Preparation of crude polysaccharides from fruiting bodies of Cordyceps militaris: collecting fruiting bodies of Cordyceps militaris, drying in the shade, pulverizing with a plant grinder, and passing through a 100-mesh sieve. Add 10 times the amount of water to the pulverized fruiting body of Cordyceps militaris, stir evenly, and place in an ultra-low temperature refrigerator to freeze at -80°C for 12 hours. Quickly put into 80 ℃ water bath to thaw, water bath for 2 hours. Then 500W ultrasonic treatment for 30 minutes, and the solid matter ob...

Embodiment 2

[0049] Example 2: Preparation of heteropolysaccharide TY258 from the fruiting body of Cordyceps militaris and its anti-atherosclerosis application

[0050] When extracting and preparing the Cordyceps militaris fruiting body heteropolysaccharide TY258 of the present invention and verifying its lipid-lowering and anti-atherosclerotic activity on the low-density lipoprotein receptor knockout mouse model, the following steps were taken:

[0051] 1) Preparation of crude polysaccharides from fruiting bodies of Cordyceps militaris: collect fruiting bodies of Cordyceps militaris, dry in the shade, pulverize with a plant grinder, and pass through a 80-mesh sieve. Add 8 times the amount of water to the pulverized fruiting body of Cordyceps militaris, stir evenly, and place in an ultra-low temperature refrigerator to freeze at -80°C for 12 hours. Quickly put into 85 ℃ water bath to thaw, and water bath for 3 hours. Then 450W ultrasonic treatment for 50 minutes, filtered to obtain the so...

Embodiment 3

[0068] Example 3: Preparation of heteropolysaccharide TY258 from the fruiting body of Cordyceps militaris and its lipid-lowering application (1)

[0069] When extracting and preparing the heteropolysaccharide TY258 of the fruiting body of Cordyceps militaris of the present invention and verifying its lipid-lowering activity on the guinea pig model induced by high fat, the following steps were taken:

[0070] 1) Preparation of crude polysaccharides from fruiting bodies of Cordyceps militaris: collecting fruiting bodies of Cordyceps militaris, drying in the shade, pulverizing with a plant grinder, and passing through a 120-mesh sieve. Add 9 times the amount of water to the pulverized fruiting body of Cordyceps militaris, stir evenly, and place in an ultra-low temperature refrigerator to freeze at -40°C for 24 hours. Thaw quickly in a 90°C water bath for 1 hour. Then 200W ultrasonic treatment for 3 hours, and the solid matter obtained by filtration is the broken Cordyceps milita...

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Abstract

The invention discloses a preparation method and application of cordyceps militaris sporocarp heteropolysaccharide. The preparation method mainly comprises the following steps that cordyceps militaris sporocarp is subjected to drying, pulverization, ethanol degreasing and hot water ultrasonic extraction, and an extracting solution is subjected to concentration, alcohol precipitation, deproteinization, column chromatography, dialysis and freeze-drying, so that the cordyceps militaris sporocarp heteropolysaccharide TY258 is obtained. The cordyceps militaris polysaccharide has the efficacy of lowering lipid and resisting to atherosclerosis and can remarkably lower the cholesterol level, the triglyceride level and the low density lipoprotein level of high-fat induced atherosclerosis model mice (mice with apolipoprotein and low density lipoprotein receptors knocked out) and lipid accumulation of the livers and arcus aortae at the concentration of 25 mg / kg, lift the high density lipoprotein cholesterol level of the mice and improve activity of superoxide dismutase and lipoprotein esterolysis enzyme. The cordyceps militaris sporocarp polysaccharide can also remarkably lower the contents of plasma triglyceride, cholesterol, malonaldehyde and oxidized low-density lipoprotein of high-fat induced guinea pigs and rats.

Description

technical field [0001] The invention relates to a bioactive substance and its preparation method and application, in particular to a heteropolysaccharide in the fruit body of Cordyceps militaris, its preparation method and its lipid-lowering application. Background technique [0002] Cardiovascular and cerebrovascular disease is a disease with high morbidity, high disability rate and high mortality rate. The main pathological process is that on the basis of vascular wall lesions, changes in blood components and / or hemodynamics lead to ischemia Sexual or bleeding disorders. There are many causes, one of which is atherosclerosis. [0003] Hyperlipidemia is one of the main factors inducing atherosclerosis. When cholesterol in the body is too high, especially when the level of low-density lipoprotein (LDL-C) and triglycerides increase, the oxidized LDL can be phagocytized by macrophages, and then deposited under the arterial endothelium, causing subendothelial degeneration, wh...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/06A61P9/10
CPCC08B37/006
Inventor 郭守东
Owner TAISHAN MEDICAL UNIV
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