Hepcidin antibiotic peptide gene of weever cultured in sea water

A technology for marine aquaculture and antimicrobial peptides, applied in genetic engineering, plant genetic improvement, peptide sources, etc., can solve the problems of difficulty in obtaining antimicrobial peptides, high cost, etc.

Inactive Publication Date: 2005-06-29
XIAMEN UNIV
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  • Abstract
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Problems solved by technology

Therefore, antimicrobial peptides will be a kind of antibacterial drugs with broad application prospects in the prevention and treatment of marine fish diseases; however, natural antimicrobial peptides are extracted directly from fish or artificially synthesized by chemical methods, which are limited by animal sources and chemical synthesis methods , it is difficult

Method used

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  • Hepcidin antibiotic peptide gene of weever cultured in sea water
  • Hepcidin antibiotic peptide gene of weever cultured in sea water
  • Hepcidin antibiotic peptide gene of weever cultured in sea water

Examples

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Example Embodiment

[0067] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings:

[0068] 1. Extraction of Liver RNA from Infected Sea Bass

[0069] Weigh 100 mg sea bass liver tissue, grind it into powder with liquid nitrogen, and add it to a nuclease-free centrifuge tube containing 1 mL Trizol. Centrifuge at 12000g for 10min at 2-8°C, suck out the pink supernatant, incubate at room temperature for 5min, completely remove the nucleoprotein complex, then add 200μL chloroform, and close the lid tightly. Shake vigorously for 15 sec, incubate at room temperature for 2-3 min, and centrifuge at 12,000 g for 15 min at 2-8°C. Take the supernatant to another nuclease-free centrifuge tube, add 500 μL isopropanol, and mix gently to precipitate RNA. Incubate at room temperature for 10 minutes, centrifuge at 12,000 g for 10 minutes at 2-8°C, and a gel-like precipitate can be seen at the bottom of the centrifuge tube, which is RNA. Discard th...

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Abstract

A hapcidin recombinant technology gene of blue pickerel, relates to the cloned hepcidin recombinant technology of blue pickerel.Using the liver RNA of blue pickerel as the model, consult the genetic sequence of cross blue pickerel hepcidin and human hepcidin to design the specific primer, makes use of RT-PCP, 5 race and 3race and so on, belongs to a new member in the hepcidin genetic family. With activity of antifungal, gram - positive bacteria and gram - negative bacteria, it is an iron adjusting hormone relative to iron metabolism, can be used to form expressive carrier of eukaryon for building genetic engineering metabolism for expressing hepcidin highly effective with feed immune additive, for producing hepcidin bacteria resistant peptide gene of blue pickerel in large quantity; to be feed immune additive, decreasing or eliminating the accumulation of antibiotics in aquatic products, increasing the quality of aquatic products; to be the effective replacement of antibiotics with chemicals proof for medicine of germ with chemicals proof in cultivating fishery and breeding research of transgenetic fish.

Description

technical field [0001] The invention relates to a hepcidin antimicrobial peptide gene cloned from sea bass (Lateolabrax japonicus). Background technique [0002] Mariculture perch (English name Japanese seabass, Latin name Lateolabrax Japonica, commonly known as flower perch, seven-star perch, perch board) is an important sea-farming economic fish. In recent years, the culture status of perch has been rising continuously in our country, and the cultivation of perch in net cages and ponds has been carried out along the coast of the country. However, with the increase of breeding density, the phenomenon of eutrophication in the breeding water body is becoming more and more serious, which leads to the deterioration of the breeding environment, and the continuous outbreak of viral diseases, bacterial diseases, fungal diseases and parasitic diseases, which not only cause huge economic losses, but also seriously contus increased people's enthusiasm for farming. [0003] Antibact...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/10C12N15/12
Inventor 王克坚周红玲杨明
Owner XIAMEN UNIV
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