6 results about "Mtt method" patented technology

The application discloses the application of Huaxingdan in the preparation of a glioma drug in the technical field of biology and medicine, detects the influence of Huaxingdan on the proliferation activity of glioma cells U251, U87 and A172 by using an MTT method in vitro, finds that Huaxingdan has a significant inhibitory effect on the proliferation of glioma cells, and is time and concentration dependent. The influence of Huaxingdan on the apoptosis of glioma cells U87 is detected by using a flow cytometry, and it is found that Huaxingdan can induce the apoptosis of U87 cells, and is concentration dependent. The animal experiment shows that Huaxingdan can effectively inhibit the growth of glioma cells in vivo, and the HE staining shows that Huaxingdan has no toxicity in vivo, and can be used as a drug for preventing and treating glioma.

The application discloses an immune regulation pharmaceutical effect analysis method based on a concentrated pill of psammochloa and astragalus, which comprises the following steps: raw material extraction, animal model construction, multi-dimensional detection and data statistics. The raw materials of psammochloa and astragalus are weighed according to a specific ratio, and then are prepared into extract by ethanol decoction and concentration. After the experimental animals are grouped and administered intragastrically, the individuals are screened by the four oxygen pyrimidine modeling. The immune organs are separated, weighed, and the index is calculated. The contents of various cytokines in serum and liver tissue are detected by the ELISA method. The tissue morphology, lymphocyte proliferation and protein expression are observed by HE staining, MTT method and immunofluorescence method. The expression of key signal pathway proteins is detected by electrophoresis and other steps after the total protein is extracted, and statistical analysis is carried out. The method is systematic and comprehensive, the result is accurate and reliable, and a scientific basis is provided for the immune regulation pharmaceutical effect evaluation of the concentrated pill.

The invention discloses application of an AKT inhibitor or an STAT3 inhibitor in preparation of a medicine for overcoming drug resistance of bladder cancer to an MEK inhibitor, and belongs to the field of biological medicine. Detecting the cell activity through an MTT method; a Western blot experiment is adopted to detect a key target protein or gene expression level, a bladder cancer drug-resistant cell ERK abnormal activation + STAT3 / Akt compensation activation mechanism is defined, p-ERK, p-STAT3 and p-Akt are locked as targets, the drug resistance of bladder cancer to an MEK inhibitor is overcome through an AKT inhibitor or an STAT3 inhibitor, the scheme can accurately inhibit abnormal pathways, reverse drug resistance and improve treatment sensitivity, and the method is safe and suitable for clinical application. A new direction is provided for patients with drug-resistant bladder cancer, and the clinical bottleneck of the MEK inhibitor is helped to be broken through.

The invention belongs to the technical field of biological medicines, and particularly relates to a 2-(substituted) phenyl-2H-indazole7-carboxamide compound as well as a preparation method and application thereof. The antitumor cell proliferation activity of the compound is evaluated by adopting an MTT method cancer cell proliferation inhibition experiment, and experimental data shows that the compounds with the structures as shown in the formula I and the formula II provided by the invention show clear and relatively strong antitumor cell proliferation activity; the conclusion proves that the 2-(substituted) phenyl-2H-indazole7-carboxamide compounds with the structures as shown in the formula I and the formula II have the possibility of being developed into novel anti-tumor therapeutic drugs.

The invention discloses an enantiomer-kaurane diterpene compound derivative and application thereof in preparation of anti-tumor metastasis drugs or inhibitors. According to the invention, it is determined that the DKA derivative has no obvious cytotoxic activity through an MTT method; tannwell cell experiments and scratch experiments prove that compared with DKA, the activity of the designed and synthesized DKA derivative for inhibiting cell migration is remarkably enhanced.

The invention discloses a method for separating compounds from persistent calyx of physalis alkekengi. Belongs to the field of physalis alkekengi persistent According to the invention, physalin compounds 1, 2 and 3 separated from petroleum ether and ethyl acetate parts of persistent calyx of physalis alkekengi are screened by adopting an MTT (Methyl Thiazolyl Tetrazolium) method. The physalin compound 1 and the physalin compound 2 provided by the invention have the growth inhibition rates of 19.61% and 15.20% on tumor cells MDA-MB-231 respectively, and have a relatively weak growth inhibition rate on the tumor cells; the growth inhibition rate of the compound 3 on tumor cells SW480 is 13.18%, and the compound 3 has weak growth inhibition on the cells.