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139 results about "Mtt method" patented technology

MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since these types of materials are expected to stimulate or inhibit cell viability...

Pharmaceutical composition having function cancer of the lungs against

A medicine combination has the function of resisting lung cancer. The combination is mixed with rhizoma paridis saponin and milk vetch root amylose according to the weight ratio of 3 to 1. The rhizoma paridis is extracted by alcohol and then the rhizoma paridis extract is obtained through gradient elutriation of macroporous absorption resin alcohol; the milk vetch root is extracted by water and then the protein is settled and removed to get the milk vetch root amylose; the two extracts are mixed and the medicine is made. The MTT activities experiment in vitro with the MTT method has proven that the medicine combination can obviously control the growth of various lung cancer cells such as LA795 lung adenocarcinoma cell and the IC50 can reach 26.73 ug/ml; the experiment of mouse with the lung cancer tumor has represented that the tumor constraint rate can reach 55.63 percent; in this way, lung transfer of hypodermic transplanted tumor of the mouse with the lung cancer tumor can be obviously controlled and the tumor cells can be brought to death; moreover, spleen index and thymus index can be promoted and the medicine is innocuous and has no side effect. In addition, compared with the raw materials, the medicine combination has high activity and clear function; moreover, the medicine can be made into different types.
Owner:TIANJIN UNIV

MTT (thiazolyl blue) cell toxicity test method of biological assessment of total particle matter in cigarette smoke

The invention relates to an MTT (thiazolyl blue) cell toxicity test method of biological assessment of a total particle matter in cigarette smoke and belongs to the technical field of safety assessment of tobacco and cigarette smoke. The MTT cell toxicity test method is characterized by comprising the following steps of: inoculating and culturing immortalized human bronchial epithelial cells (BEAS-2B cells) and contaminating the total particle matter in the cigarette smoke; detecting the cell survival rate by adopting an MTT method; and analyzing and evaluating the cell toxicity of the total particle matter in the cigarette smoke according to a test result. Compared with the prior art, the MTT cell toxicity test method has the following characteristics that BEAS-2B cells are human cells and are target organ source cells acting on a human body by the smoke; a BEAS-2B cell system is used for carrying out cell toxicity assessment on the cigarette smoke and has the strong pertinence; in a testing process, steps of washing the cells for a plurality of times are reduced; and a formaldehyde fixing step does not need to be carried out so that a testing period is shortened, the operation is rapid and convenient, the sensitivity is high and the result is stable and reliable. Moreover, the MTT test method disclosed by the invention is further applicable to a smoke cell toxicity test of various cell systems and the commonality is strong.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Fluorescence-labeled magnetic kaempferol microsphere system and preparation method thereof

The invention discloses a fluorescence-labeled magnetic kaempferol microsphere system and a preparation method thereof. The preparation method comprises the following steps: (1) firstly, dissolving a hydrophobic drug, namely hydrophobic drug, in an oil-base modified magnetic fluid, uniformly mixing, dropping the dissolved kaempferol to a proper amount of a bovine serum albumin solution by virtue of an injector, and synthesizing a magnetic kaempferol protein microsphere through an ultrasonic chemical method; (2) then, connecting to self-made graphene quantum dots through an amino carboxylic reaction so as to obtain a fluorescent and magnetic dual-functional kaempferol microsphere; and (3) co-cultivating the a fluorescent and magnetic dual-functional kaempferol microsphere with human Hela cells, and detecting cytotoxicity by virtue of an MTT method. The fluorescence-labeled magnetic kaempferol microsphere disclosed by the invention is low in preparation cost and high in drug loading rate; the microsphere not only has the superparamagnetism of magnetic particles but also reserves the excellent fluorescence performance of the graphene quantum dots; and the microsphere can achieve an excellent effect on medicines for tumor targeted therapy.
Owner:南京美材科技有限公司

Preparation method and application of nano diamond targeted medicine

The invention provides a preparation method and application of a Tf (transferrin) modified nano diamond targeted medicine. The preparation method of the nano diamond targeted medicine comprises the following steps: dissolving transferrin Tf into a PBS (phosphate buffer solution), then adding DOX (doxorubicin) and reacting to obtain a Tf-DOX compound; and carrying out reaction on carboxylated nano diamond and the Tf-DOX compound to obtain the nano diamond targeted medicine ND-(Tf-DOX). The nano diamond targeted medicine acts on HepG2, and treatment on cells by virtue of low temperature, different inhibitors and free Tf shows that the nano diamond targeted medicine has energy dependence; with normal human HEK293 as reference, study on absorption of the cells for the nano diamond targeted medicine shows that the nano diamond targeted medicine has targeted selectivity; with human HepG2 and human HeLa as references, detection on cytotoxicity of the nano diamond targeted medicine by adopting an MTT method shows that the nano diamond targeted medicine has a targeted anti-tumour effect and selectivity for cancer cells; and study on a tumour inhibiting effect of the nano diamond targeted medicine in vivo by taking tumour-bearing mice as models shows that the nano diamond targeted medicine can be used for inhibiting tumours and has low toxic and side effects on DOX. Therefore, the nano diamond targeted medicine can be applied to preparation of an anti-tumour medicine.
Owner:SHANXI UNIV

Screening method of esophagus cancer markers by tea polyphenol in tea and golden camellia

The invention discloses a screening method of esophagus cancer markers by tea polyphenol in tea and golden camellia. The tea polyphenol in tea and golden camellia is extracted and prepared from different types of tea and golden camellia, and the tea polyphenol acts on human esophageal squamous cells and adenocarcinoma cell OE33 which are cultured in vitro; an anti-proliferative effect on esophagus cancer by the tea polyphenol is detected in an MTT method, and an inducing differentiation effect on the esophagus cancer by the tea polyphenol is detected by flow cytometry so as to observe cell cycles and apoptotic effects; cell morphological change is observed by using a fluorescence inversion microscope; protein expression change in the esophagus cancer cells is analyzed by means of immunohisochemistry, Real-time fluorescent quantitation RT-PCR and Westem-blot. Based on SPSS1 statistics, the detection results are analyzed, and differential expression proteins obtained by performing action on the esophagus cancer by the tea polyphenol in the tea and golden camellia are detected, and associated proteins are screened out according to database search and matching. In the invention, a mechanism of the function of tea polyphenol can be expounded in accordance with the research on marker molecules of the differential expression proteins acted by the tea polyphenol.
Owner:程金生

Method for quickly measuring total number of live bacteria of luminous bacteria by using MTT method

The invention aims at establishing a method for quickly measuring total number of live bacteria of luminous bacteria, in particular to a method for counting the live bacteria based on the MTT coloration principle. The invention adopts MTT colorimetric method to measure the viable count of the luminous bacteria and carries out optimizing selection on the detection condition of the MTT colorimetric method. Therefore, the optimal method for measuring total number of the live bacteria of the luminous bacteria is determined. In the invention, first, the product of formazan generated from the reaction of succinate dehydrogenase in MTT and live thalli is dissolved in DMSO, light absorption value is determined within the range of 400nm-800nm, and the largest detected absorption wavelength is determined to be 500nm. By optimizing time and influencing the light absorption value by temperature, the optimal measurement condition is detected: the reaction time is 2h, and the reaction temperature is 25 DEG C. The invention has novel detection principle, simple and fast detection method, good repeatability, can be used for the quantitative detection of the number of the live bacteria of the luminous bacteria in the fields of food hygiene and safety, environmental monitoring and the like. The invention can also be applied to the tests of microbiology, immunology and other disciplines and used for measurement of the activity and number of the live bacteria materials. The invention can also develop the method for measuring the total number of the bacteria which is used for replacing the traditional method for measuring the total number of the live bacteria.
Owner:OCEAN UNIV OF CHINA

Preparation and application of magnetic graphene drug-loading system

The invention discloses a preparation method of a magnetic graphene drug-loading system. The preparation method comprises the following steps of: (1) modifying oxidized graphene by using two types of surface active agents, and synthesizing into magnetic oxidized graphene by a hydrothermal synthesis method; (2) modifying by an amphiphilic polymer PF127, reducing the magnetic oxidized graphene into magnetic graphene with good biocompatibility by hydrazine hydrate; (3) loading a hydrophobic anti-cancer drug (paclitaxel) onto the magnetic graphene by pi-pi stacking effect and preparing into the magnetic graphene drug-loading system with integration of an anti-cancer effect, imaging and biocompatibility; (4) culturing the magnetic graphene drug-loading system and MCF-7 breast cancer cells together, and detecting the cell toxicity by an MTT method. The invention discloses application of the magnetic graphene drug-loading system in treating the breast cancer. The magnetic graphene drug-loading system disclosed by the invention is good in drug dispersity, strong in magnetic responsibility, good in biocompatibility and high in drug loading amount, and can show better inhibiting effect for MCF-7 breast cancer cells under the condition of less use amount (200mug / mL).
Owner:JINLING INST OF TECH

Applications of anthocyanin and regulation for CHOP gene in preventing and treating atherosclerosis

The invention discloses the use of anthocyanin in medicines for treating atherosclerosis and the application for preventing and treating atherosclerosis with regulation and control of CHOP gene, and relates to the improvement effect of anthocyanin monomer cyanidin-3, 5-diglucoside on macrophage injuries caused by oxidized low density lipoprotein, and discusses the mechanism of action thereof. In the invention, mouse macrophage cell line RAW264.7 is tested, and the effect of cyanidin-3, 5-diglucoside on activity of RAW264.7 macrophage processed by the oxidized low density lipoprotein is determined based on MTT method, the total RNA of cells is extracted, Affymetrix Mouse U4302.0 gene expression profile chip is applied to carry out marking hybridization experiments, differential gene analysis is carried out for the scanning results of Affyemtrix gene chip with MTT method, and the differential genes are screened, and further real-time quantitative PRC method is utilized to further validate the expressed differential gene, The validation results verify that the cyanidin-3, 5-diglucoside can regulate CHOP gene expression down, and has obvious protective action on macrophages injured by oxidized low density lipoprotein.
Owner:WUHAN UNIV

Oyster polypeptide-nano selenium granule compound having hangover-relieving and liver-protecting effects and preparation method and application thereof

The invention discloses an oyster polypeptide-nano selenium granule compound having hangover-relieving and liver-protecting effects and a preparation method and application thereof. The method includes: homogenizing oyster meat, adding water and active enzyme, enzymolyzing, allowing boiling water bath, centrifuging to take supernate, cold-drying the supernate to obtain oyster polypeptide powder, adding the oyster polypeptide powder into water, well mixing to obtain a solution, adding a sodium arsenite solution into the oyster polysaccharide solution, well mixing, adding an ascorbic acid solution while stirring, allowing water bath reaction, dialyzing to obtain functionalized nano selenium sol, ultrasonically treating, and freeze-drying to obtain the oyster polypeptide-nano selenium granulecompound. Oyster polypeptide is used to stabilize nano selenium, so that stability of nano selenium is remarkably improved, and nano selenium can be stably stored at 4 DEG C for one month; through MTT method detection, nano granules can relieve damage effect of alcohol or hydrogen peroxide on liver. If the compound is converted into healthcare products having a liver protecting function and capable of supplementing selenium element, great social and economic benefit can be generated.
Owner:SOUTH CHINA UNIV OF TECH +1

Preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity

The invention relates to a preparation method for Sinkiang turnip polysaccharide having immunoregulatory activity. The method includes the steps that Sinkiang turnip root tubers are dried and smashed, ethyl alcohol with the concentration of 80% is used for removing impurities from the Sinkiang turnip root tubers, the Sinkiang turnip root tubers are subjected to hot water extraction, suction filtration is conducted to extract supernate, absolute ethyl alcohol precipitation and centrifugation are conducted, precipitate is washed sequentially through diethyl ether, ethyl alcohol and acetone and is dried until the constant weight state is reached, and the Sinkiang turnip polysaccharide is obtained. An MTT method is used for analyzing the immunoregulatory activity of the Sinkiang turnip polysaccharide and it is discovered that the Sinkiang turnip polysaccharide has a remarkable effect of promoting mouse peritoneal macrophage proliferation. The preparation method for the Sinkiang turnip polysaccharide has the advantages that the extraction yield is high, cost is low, the structure of the polysaccharide is complete, and no pollution is caused. The prepared Sinkiang turnip polysaccharide has the prominent immunoregulatory activity, thereby having broad application prospects in the food industry and the pharmaceutical industry.
Owner:NANJING AGRICULTURAL UNIVERSITY

Preparation of carbon quantum dot fluorescent probe and application of carbon quantum dot fluorescent probe in selective detection of active oxygen

The invention belongs to the technical field of nanomechanics and molecular biology, and relates to preparation of a carbon quantum dot fluorescent probe and application of the carbon quantum dot fluorescent probe in selective detection of active oxygen. Carbon quantum dots with specific detection functions on different active oxygen can be prepared by the method. A series of different carbon sources are used as precursors, and different carbon quantum dots are synthesized through a one-step hydrothermal reaction. XPS characterization shows that the types and contents of oxygen-containing groups and nitrogen-containing groups on the surfaces of the carbon quantum dots generated by different carbon sources are different, and various groups have different binding capacities with active oxygen such as superoxide anions (O2<.->) and hydroxyl radicals (.OH), so that the carbon quantum dots can be used for specific detection of related active oxygen. Meanwhile, an MTT method proves that thecarbon quantum dot synthesized by the method has good biocompatibility, can be used for in-vivo cell imaging and online detection, detects the content of active oxygen generated by stimulating livingcells by a drug, and is used for related cell counting.
Owner:EAST CHINA UNIV OF SCI & TECH
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