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Colloid selenium test paper of semi-quantitative determination oxidized low density lipoprotein

A low-density lipoprotein, semi-quantitative detection technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of cumbersome preparation methods of gold particles, the inability to realize single-part detection, and the long time required for detection, etc., to achieve The detection process is fast and fast, the detection time is short, and the effect of less reagent and sample volume

Active Publication Date: 2008-03-12
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages of this method are: (1) special equipment such as a microplate reader is required for use; (2) detection operators need to undergo professional training; (3) the operation process is relatively complicated, and the time required for detection is relatively long ; (4) The cost required for detection is relatively high, and a single detection cannot be realized
Therefore, many researchers are currently focusing on colloidal gold test strips for detecting oxidized low-density lipoprotein in human serum, such as the invention patent 200510200394.6, but the preparation method of gold particles is cumbersome, requires heating, and takes a long time. And the amount of labeled protein is more

Method used

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  • Colloid selenium test paper of semi-quantitative determination oxidized low density lipoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The preparation method of the colloidal selenium test strip for the semi-quantitative detection of oxidized low-density lipoprotein by spraying OX-LDL monoclonal antibody 2 on the detection belt:

[0025] 1. Preparation of colloidal selenium particles

[0026] Add ascorbic acid, selenous acid and ascorbic acid in sequence in the reactor, add selenous acid and ascorbic acid at intervals from the fourth minute of the reaction, and then scan the spectrum. It is advisable to add 10% Dextran terminated the reaction, and the product was collected for future use.

[0027] 2. Preparation of monoclonal antibodies

[0028] BALB / C mice were immunized with OX-LDL, the splenocytes of the immunized mice were hybridized and fused with mouse myeloma cells to prepare hybridoma cells, and the cell lines capable of stably secreting OX-LDL monoclonal antibodies were screened and cultured to a certain density in vitro , and then inoculated into the peritoneal cavity of BALB / ...

Embodiment 2

[0037] Embodiment 2: A method for making a colloidal selenium test strip for semi-quantitative detection of oxidized low-density lipoprotein by spraying rabbit anti-mouse IgG on the detection strip.

[0038] 1. Preparation of colloidal selenium particles

[0039] Add ascorbic acid, selenous acid and ascorbic acid in sequence in the reactor, add selenous acid and ascorbic acid at intervals from the fourth minute of the reaction, and then scan the spectrum. It is advisable to add 10% Dextran terminated the reaction, and the product was collected for future use.

[0040] 2. Preparation of monoclonal antibodies

[0041] BALB / C mice were immunized with OX-LDL, the splenocytes of the immunized mice were hybridized and fused with mouse myeloma cells to prepare hybridoma cells, and the cell lines capable of stably secreting OX-LDL monoclonal antibodies were screened to prepare anti-OXLDL monoclonal antibodies. Cloning the antibody and determining the titer of the monoclonal antibody...

Embodiment 3

[0052] Embodiment three: the using method of colloidal selenium test strip of the present invention

[0053] 1. Collect samples:

[0054] Blood was drawn from the forearm on an empty stomach at 7:10 in the morning, and antioxidants were added immediately to prevent low-density lipoproteins from continuing to oxidize in vitro. After centrifugation, the plasma was collected and placed at -30°C for OX-LDL testing.

[0055] 2. Detection

[0056] Take the test strip out of the package and let it stand at room temperature for about 5 minutes. Take out the test paper, immerse the detection area in the sample solution, and the sample liquid level should not exceed the detection area. Take it out after 3-5 seconds and place it horizontally on a clean operating table. Results can be seen with the naked eye within 6-10 minutes.

[0057] (1) The test results of colloidal selenium test strips for the semi-quantitative detection of oxidized low-density lipoprotein with OX-LDL monoclonal...

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Abstract

The present invention provides a piece of semiquantitative detecting oxide low-density lipoprotein (OX-LDL) colloid selenium reagent paper, which can capture antigen quantity according to OX-LDL monoclonal antibody to create a relation between color and standard OX-LDL and diagnose that content of OX-LDL in human blood plasma is higher than, lower that or equal to standard OX-LDL quantity in human blood plasma, thus realizing early auxiliary diagnosis to ASCVD. The present invention has the advantages of convenience, quickness, sensitivity, specificity and economic efficiency, rejects specified instruments and equipments and is conveniently taken.

Description

1. Technical field [0001] The invention relates to a colloidal selenium test strip capable of rapidly and semi-quantitatively detecting oxidized low-density lipoprotein and its content in plasma samples, which can be applied to early auxiliary clinical diagnosis of atherosclerotic cardiovascular disease. 2. Background technology [0002] Many studies have shown that oxidized low-density lipoprotein (OX-LDL) has the effect of causing atherosclerosis (AS). And atherosclerosis is the early pathological basis of coronary heart disease, hyperlipidemia, angina pectoris, myocardial infarction and other cardiovascular diseases. The role of OX-LDL in AS is firstly to promote the formation of foam cells and act as an inflammatory mediator in the early stage of AS formation. Secondly, OX-LDL is highly cytotoxic to vascular endothelial cells (VEC) and vascular smooth muscle cells (VSMC), changing the morphology and structure of VEC and destroying the integrity of the endothelium, allow...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543
Inventor 李红玉何丽娟
Owner LANZHOU UNIVERSITY
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