627 results about "Semi quantitative" patented technology

Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and / or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and / or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA-tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.

The invention provides a quantitative characterization method of low penetration double-medium sandstone oil reservoir microscopic aperture structure, which comprises the following steps: selecting experiment samples, effectively combining various experiments, distributing samples, processing and analyzing experiment test data, combining macroscopic background and microscopic rock core, and combining static state analysis and dynamic production reality, thereby realizing quantitative characterization of ultra-low penetration double-medium sandstone oil reservoir microscopic aperture structure from qualitative analysis and semi-quantitative evaluation. The invention has the advantages that more comprehensive influence factors and micro crack, aperture throat parameter and nuclear magnetic resonance movable fluid parameter are considered, so the characterization result can better reflect change characteristics of ultra-low penetration double-medium sandstone oil reservoir microscopic aperture structure, which are consistent to the production exploitation real cases of the oil field, thereby effectively avoiding one-sidedness and limitation of single aspect evaluation result.

The present invention provides a method and a device that utilizes capillarity-mediated, chromatographic transport, for the qualitative or semi-quantitative analysis of selected analytes in liquid samples. The device utilizes an applicator / collection device for collecting and administering the sample to the flow path such that reagent(s) flow through the applicator / collection device, washing the sample into the reaction pathway. The device farther utilizes an air gap between the initial location of the reagent and the reaction pathway to funnel the reagent efficiently through the sample so as to collect all or substantially all of the sample and make it available for the reaction(s).

Disclosed herein is a system and method for providing remotely accessible medical image data. The system and method allows for increased accuracy and semi-quantitative or fully quantitative data from images by enabling the remote user to select regions of interest on a compressed image, and then conducting quantitative analysis on original images at a central location.

The present invention relates to a simple immunochemical semi-quantitative assay method according to chromatography, which comprises trapping a certain amount of an analyte in a sample with a predetermined amount of a fixed antibody for the analyte before qualitative analysis of the analyte, the certain amount corresponding to the amount of the fixed antibody, and thereby decreasing a concentration of the analyte to be subjected to subsequent immunochemical qualitative determination, and an apparatus therefor.

A lateral flow device for testing a bodily fluid, such as urine, blood, mucous, saliva, etc., is provided. The lateral flow device is configured to be attached to an absorbent article via an attachment mechanism located on the lateral flow device or the absorbent article. The lateral flow device includes a chromatographic medium (e.g., porous membrane) that defines a detection zone that provides a signal indicative of the presence or absence of the analyte. The device may also include a control zone that provides a signal indicative of whether a sufficient amount of bodily fluid has been provided and tested. Regardless of its specific configuration, the lateral flow device is configured to be attached onto the absorbent article to provide a user or caregiver with rapid information about a health condition. For example, the device may be attached onto a diaper to provide information about the presence of enzymes or other compounds often encountered with a patient having a urinary tract infection. This information may provide an early warning system to allow the user or caregiver to seek additional testing and / or treatment. Alternatively, semi-quantitative or quantitative results may be derived from the test.

An absorbent article that contains a lateral flow assay device for testing a bodily fluid, such as urine, blood, mucous, saliva, etc., is provided. The lateral flow assay device includes a chromatographic medium (e.g., porous membrane) that defines a detection zone that provides a signal indicative of the presence or absence of the analyte. The device may also include a control zone that provides a signal indicative of whether a sufficient amount of bodily fluid has been provided and tested. Regardless of its specific configuration, the lateral flow assay device is integrated into the absorbent article to provide a user or caregiver with rapid information about a health condition. For example, the device may be integrated into a diaper to provide information about the presence of enzymes or other compounds often encountered with a patient having a urinary tract infection. This information may provide an early warning system to allow the user or caregiver to seek additional testing and / or treatment. Alternatively, semi-quantitative or quantitative results may be derived from the test.

Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and / or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and / or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA−tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.

The invention relates to a detection method, in particular to a rapid detection method employing ultrasensitive quantum dot microsphere immunity-chromatograph test paper strips. The prior art has the disadvantage that the detection sensitivity is unable to satisfy demands of users because of high detection limit of samples, complex composition of the samples and great pretreatment difficulty. The rapid detection method of the invention comprises the steps of: preparing silicon dioxide nanoparticles, incubating the amino silicon dioxide nanoparticles with antibody for 1-10 hours in the presence of a condensation agent at 37 DEG C, and removing the antibody and the condensation agent which are failed to bond 1-4 times through high-speed centrifugation, redissolving precipitates obtained after centrifugation, adding the condensation agent and carboxylic water-soluble quantum dots, and removing free quantum dots and the condensation agent through high-speed centrifugation; preparing a silica / quantum dot composite microsphere probe; and constructing an immunity-chromatograph test paper strip system. The invention has the advantages of simple and convenient detection method, accurate and visual results, high sensitivity, low price, and wide application and can be used for semi-quantitative detection and quantitative detection.

Disclosed is a CMOS image sensor-based on quantum dot marking test strip quantitative detection system, which comprises a quantum dot marking test strip, a test strip frame, a scanning system, a CMOS image sensor, a data-processing device, an output display device, a printer, a keyboard and an IC card matched with the test strip. After reading the IC card parameters, the data-processing device controls the test strip to move, so that the scanning system can automatically scan a detection band and a quality control band of the test strip; the collected signals are transmitted to the data-processing device through the CMOS image sensor, so that the optical density of the detection band and the quality control band can be identified and processed with concentration computing and technical analysis; and the test results are transmitted to the output display device and displayed. The invention can accurately achieve one-component and multi-component quantitative, semi-quantitative or qualitative detection of samples and can dynamically monitor the immunochromatographic reaction process. The system has high sensitivity, objective result and flexible operation.

The invention relates to the technical field of environmental protection or chemistry analysis, which is an on-site quick detection method for organic pollutants in water; wherein on-site quick separation is carried out on various types of organic pollutants in a polluted water sample to be detected with thin layer chromatography, and on-site quick detection is carried out on the organic pollutants by using surface enhancement Raman spectroscopy technology. The method comprises the following steps of: firstly, preparing the active base of a high-density surface reinforced Raman spectrum; secondly, establishing a characteristic-peak strength-density standard curve map; thirdly, separating the organic pollutants which are stabilized on a chromatographic sheet; fourthly, obtaining the surface reinforced Raman spectrum map of the organic pollutants; and fifthly, carrying out contrast and detection. The method has the following benefits: the method has high detection and analysis speed, high sensitivity, wide detection range, low sample dosage and convenience for instrument carrying and operation; and the on-site quick qualitative analysis and semi-quantitative detection can be carried out on the organic pollutants, wherein the detective sensitivity can be less than 1 ppm.

The invention provides a device comprising one or more support materials capable of providing lateral flow. The one or more support materials contain an area for a receiving a biological sample containing a target analyte, an area having a movably contained detector ligand capable of forming a complex with the target analyte, a first capture area having a predetermined amount of an immobile capture reagent, where the immobile capture reagent is capable of specifically binding to the complex, a second capture area having the immobile capture reagent, and a lysing agent. Typically, the area for receiving the biological sample, the area having the movably contained detector ligand, the first capture area, and the second capture area are arranged in the one or more support materials such that a sample is capable of sequential lateral flow through these areas.

Disclosed herein is a system and method for providing remotely accessible gene expression image data. The system and method allows for increased accuracy and semi-quantitative or fully quantitative data from images by enabling the remote user to select regions of interest on a compressed image, and then conducting quantitative analysis on original images at a central location. The subject invention relates to, in one embodiment, an BVIGEM (Interactive Multiple Gene Expression Maps) system: which provides internet based software tools for the extraction of functional information from gene expression images and also to act as a repository for gene expression image data.

The invention belongs to the field of nucleic acid detection and discloses a method for semi-quantitatively detecting pathogenic nucleic acid by adding internal control nucleic acid. Corresponding internal control is added in the whole process of extracting and amplifying target nucleic acid and testing by using a test paper, so that the internal control and a target segment are parallelly operated, and the semi-quantitative detection is performed finally through color development and intensity contrast of three strips, namely a detection line, an internal control line and a quality control line on the test paper. In the method, in the whole process of processing the target nucleic acid, the corresponding internal control is taken as a positive contrast, and false negative results due to links such as extraction, amplification or sample application errors are avoided in the processing of detecting by using the test paper. Meanwhile, by comparing color development intensity of the internal control line and a sample line and introducing the semi-quantitative function on the basis of the qualitative function of the immunochromatographic test paper to estimate the copy number of tested samples, the detection results are more detailed, accurate and reliable. The method has the advantages of convenient and quick operation and capacity of meeting the actual clinical requirement.

The invention discloses preparation of hydrophilic polymer and application thereof in detecting mercury ions based on the change of fluorescence and color, particularly a rhodamine B hydrazide grafted hydrophilic polymer probe applied to the detection of mercury ions in aqueous medium based on the change of fluorescence and color and a kit and test paper applied to the detection of the mercury ions by using the probe, belonging to the technical field of material preparation and heavy metal ion detection. The polymer containing a rhodamine chromophore is prepared by subjecting rhodamine B hydrazide and propylene oxide on the side chain of polyvinylpyrrolidone-glycidyl methacrylate which is a water-soluble polymer to ring-opening reaction, has good water solubility and can be applied to selectively detecting the mercury ions by using visual colorimetry and fluorescence detection. The probe realizes the high-sensitivity and high-selectivity rapid quantitative or semi-quantitative detection of the trace mercury ions in the aqueous medium, has a simple synthetic route, low cost and convenience of use, is applicable to amplified synthesis and actual production and has great application prospects in the field of medicine, biology and environmental science and the like.

An absorbent article that contains a lateral flow assay device for testing a bodily fluid, such as urine, blood, mucous, saliva, etc., is provided. The lateral flow assay device includes a chromatographic medium (e.g., porous membrane) that defines a detection zone that provides a signal indicative of the presence or absence of the analyte. The device may also include a control zone that provides a signal indicative of whether a sufficient amount of bodily fluid has been provided and tested. Regardless of its specific configuration, the lateral flow assay device is integrated into the absorbent article to provide a user or caregiver with rapid information about a health condition. For example, the device may be integrated into a diaper to provide information about the presence of enzymes or other compounds often encountered with a patient having a urinary tract infection. This information may provide an early warning system to allow the user or caregiver to seek additional testing and / or treatment. Alternatively, semi-quantitative or quantitative results may be derived from the test.

Disclosed is an improved method for determining the concentration of a first analyte in a fluid test sample as a function of a second analyte also present in the sample whose concentration in the fluid sample is clinically related to that of the first analyte. The method involves determining the concentration of the first analyte, and, if this concentration is outside of its useful analytical range, dividing this concentration by the normal concentration of the second analyte. This method of ratioing the concentrations of the first and second analyte is advantageous because accuracy is increased with fewer false positive and false negative results being reported.

Disclosed herein is a system and method for providing remotely accessible medical image data. The system and method allows for increased accuracy and semi-quantitative or fully quantitative data from images by enabling the remote user to select regions of interest on a compressed image, and then conducting quantitative analysis on original images at a central location.

A method to serially determine changes in perfusion to tissues is provided. This method involves injecting contrast material into a catheter that is positioned in the blood supply proximal to the targeted tissue of interest, acquiring a time series of images that depicts the uptake of this contrast material within the tissue, deriving semi-quantitative or quantitative perfusion metrics based upon the time series of perfusion images, altering perfusion to the targeted tissue by means of injecting pharmacologic agents or embolic agents into the blood vessels supplying the targeted tissue, repeating the acquisition of perfusion images to serially monitor changes in tissue perfusion after each alteration, and calculating changes in perfusion metrics after each series of perfusion images. This method is used to monitor changes in perfusion to various tissues, including a diverse array of tumors. The perfusion imaging method can be acquired using magnetic resonance, x-ray computed tomography, or radionuclide imaging. The perfusion metric is serially measured during an embolization procedure as a means of measuring changes in tissue perfusion or to target an endpoint based upon a specific alteration in the calculated perfusion metric.

The invention relates to a geological logging interpretation and evaluation method for oil drilling exploration. The method is based on wellbore information and collects drilling time, oil and gas characteristics of rock samples, fluorescence characteristics, gas measurement parameters, mud tank surface conditions, storage 20 parameters of 6 categories of layer electrical measurement parameters are stored in the corresponding table, and then the scoring method is used to assign specific points to each parameter item; geological analysis experts make interpretation and evaluation conclusions based on the above parameter information to guide on-site personnel to judge the underground The properties of the reservoir fluid can achieve the goal of protecting oil and gas layers and safe drilling, and provide accurate and reliable geological parameters in time to provide a basis for the next step of work measures or construction plan formulation. The geological interpretation and evaluation method provided by the present invention analyzes and defines the parameters reflecting reservoir characteristics and fluid properties, so that qualitative data can be transformed into quantitative or semi-quantitative interpretation, and from a single evaluation to a comprehensive analysis and evaluation of multiple parameters, which can make Reservoir interpretation and evaluation results or conclusions are more scientific and rational.

The invention provides a quick detection method of tea polyphenol contents on the basis of a micro-fluidic paper chip technology. The quick detection method comprises the following steps: arranging a symmetrical hydrophilic channel network on a micro-fluidic paper chip, wherein the symmetrical hydrophilic channel network comprises a center area and a plurality of hydrophilic channels around the center area, one end of each hydrophilic channel is communicated with the center area, one end, far away from the center area, of each hydrophilic channel is provided with a reaction detection area, and the plurality of hydrophilic channels have the same length and width and form point symmetry; in each reaction detection area, dropwise adding a foline-phenol reagent and carrying out sample introduction, dropwise adding sodium carbonate solution in the center area, diffusing to each detection area through the hydrophilic channels, carrying out developing reaction for 1-10min, and carrying out semi-quantitative measurement or quantitative measurement of tea polyphenol. According to the method, a symmetrical multi-channel detection array of the micro-fluidic paper chip is used for carrying out parallel developing reaction and integral data acquisition, and a foline-phenol developing-detecting array with highly-consistent reaction time is obtained, so that accurate colorimetric determination can be realized at any time point in a developing reaction process, and time required for detection is greatly shortened.

The invention defines an all-solid-contact ISE which comprises a transducer layer of carbon nanotubes which brings the sensing layer and conducting element into contact. The invention also defines a method for the preparation of said all-solid-contact ISE and the use of the same for the qualitative, quantitative or semi-quantitative analysis of analytes. Said all-solid-contact ISE makes it possible to detect or quantify highly diverse chemical species in a reliable and reproducible manner, with the added advantages derived from its simplicity and low construction cost.

The invention relates to semi-quantitative detecting test paper of cardiac troponin, mainly comprising five parts: a sample pad, a combination pad, a water sucking pad, a soleplate and a nitric acid fibre film, wherein two parallel antibody capture lines are made on the nitric acid fibre film; one first anti-cardiac troponin I monoclonal antibody with the determined concentration close to the combination pad is used as a detecting line; another goat-anti-rabbit IgG antibody with the determined concentration is used as a standard concentration developing contrast line; the distance between the two lines is 4-7 mm; and the combination pad is sprayed with two substances of a second anti-cardiac troponin I monoclonal antibody and a gold rabbit IgG marked by colloidal gold or colour latex. The quantitative detecting test paper of cardiac troponin in the invention has the advantages of simple preparation method, low cost, convenience for detection and use, and capability of rapidly conveniently semi-quantitatively detecting the content of the cardiac troponin I in the human body blood sample, and is suitable for family application and popularization.

The invention discloses a europium-doped titanium dioxide / graphene oxide composite film and a preparation method thereof. According to the film, a substrate is provided with a europium-doped titanium dioxide nanodot and graphene oxide mixed layer, wherein titanium dioxide nanodots in the mixed layer are 30-150nm in size and 1.0*10<10>-1.0*10<11> / cm<2> in density, the molar concentration of Eu<3+> is 0.005-0.015mol / L, and the concentration of graphene oxide is 5-15mg / L. The preparation method is based on a sol-gel method and comprises the steps of preparing europium-doped titanium dioxide / graphene oxide precursor sol and then performing thermal treatment of the precursor sol on the substrate by a spin-coating method. The film provided by the invention has favorable biocompatibility, protein adsorption property and luminescence property, and can be used for in-situ semi-quantitative characterization of the protein adsorption capacity of a film surface. The film can be applied in the biomedical engineering fields of cell culture in vitro, tissue engineering and the like.

The present invention relates to a colloidal gold semiquantitative immunologic diagnosis test paper preparing and assembly method. Said test paper utilizes immunity chromatograph principle capable of rapid visual inspection nano colloidal gold marker and sample combined colour, judging detective semiquantitative result through test zone with parallel reference zone colour contrast.

The invention relates to a colloidal gold test strip for semi-quantitative detection of progesterone and a preparation method thereof. The invention belongs to the technical field of dairy cow pregnancy diagnosis. Colloidal gold test strip for semi-quantitative detection of progesterone, the sample pad, the gold pad containing gold-labeled progesterone antibody complex, and the nitrocellulose membrane are pasted end to end on the bottom plate; there are detection lines and control lines on the nitrocellulose membrane . Preparation of test strips: preparation of colloidal gold: prepared from chloroauric acid and trisodium citrate; preparation of purified polyclonal antibody: preparation of artificial antigen of dairy cow progesterone by dicyclohexylcarbodiimide coupling method; artificial antigen of progesterone was then used Immune mice to prepare progesterone polyclonal antibody; prepare gold-labeled antibody: colloidal gold and antibody adsorption, centrifugal purification; colloidal gold test strip preparation: soak gold-labeled pad with Tween-containing PB solution, gold-labeled antibody perfusion glass fiber Membrane; nitrocellulose membrane Spray bovine progesterone artificial antigen and goat anti-mouse secondary antibody into 2 lines with a film dispenser. The invention is simple in operation, convenient and practical, efficient and fast, and easy to popularize.

The invention discloses a color RGB (red, green and blue)-component-based urine analysis device, which is characterized by comprising a processor, a serial communication unit, a temperature measuring unit, a light intensity control unit and a color sensor unit, wherein the processor controls the light intensity of a white LED (light-emitting diode) lamp through the light intensity control unit, light emitted from the white LED lamp shines on a urinalysis test strip on a sampling platform, a color RGB component of reflected light of each reagent area of the urinalysis test strip is acquired by using the color sensor unit, and the processor determines a semi-quantitative value of a corresponding detection index of each reagent area according to the color RGB components and temperatures. The device and the method disclosed by the invention have the remarkable effects that the circuit structure is simple, and the cost is low; through the coordination of the color sensor unit and the temperature detection unit, a calculation coefficient of each detection index is fitted by using an experience numerical value of the existing precision instrument, so that a microprocessor can calculate an accurate measurement index by using the coefficients; the device is high in equipment precision and wide in temperature application range.

The invention provides a preparation method of urea detection test paper in dairy and a detection method of urea; urease source powder is taken and mixed with pH indicator with the pKHIn more than 6.8; the mixture is diluted by deioned water and the filter solution or the upper suspension liquid after natural deposition is taken, uniformly fixed on a carrier with good water absorption by the forms of coating, printing or soaking, and dried subsequently, thus forming the urea detection test paper; the detection method of the dairy comprises the steps as follows: firstly, common pH test paper is used for detecting the dairy to be detected; whether the dairy to be detected is in normal pH range 6.3-6.8 or not is checked; if so, the urea detection is directly carried out; if not, the pH value is adjusted to the normal pH range and the urea detection is carried out subsequently; one piece of urea detection test paper dips the dairy to be detected and the color change of the test paper is observed; if the color of the test paper is not changed, the dairy is qualified; if the color of the test paper changes, the dairy is mixed with the urea, thus carrying out qualitative judgment on the dairy; and the displayed color is compared with a standard colorimetric card, so as to carry out semi-quantitative detection of the urea in the dairy.