Quantum dot marking test strip quantitative detection system based on CMOS image sensor

Abstract

Disclosed is a CMOS image sensor-based on quantum dot marking test strip quantitative detection system, which comprises a quantum dot marking test strip, a test strip frame, a scanning system, a CMOS image sensor, a data-processing device, an output display device, a printer, a keyboard and an IC card matched with the test strip. After reading the IC card parameters, the data-processing device controls the test strip to move, so that the scanning system can automatically scan a detection band and a quality control band of the test strip; the collected signals are transmitted to the data-processing device through the CMOS image sensor, so that the optical density of the detection band and the quality control band can be identified and processed with concentration computing and technical analysis; and the test results are transmitted to the output display device and displayed. The invention can accurately achieve one-component and multi-component quantitative, semi-quantitative or qualitative detection of samples and can dynamically monitor the immunochromatographic reaction process. The system has high sensitivity, objective result and flexible operation.

Description

technical field [0001] The invention relates to a quantitative detection system for quantum dot marking test strips based on a CMOS image sensor. The system can scan, detect and interpret quantum dot-labeled test strips to realize biological detection of pathogens, antigens, antibodies, prohibited drugs, major diseases (tumor, cancer, cardiovascular disease, diabetes, etc.), pesticide residues, and food safety. Quantitative, semi-quantitative and qualitative detection of various target objects. Background technique [0002] In immunoassay, methods such as radioimmunoassay, enzyme-linked immunosorbent assay (ELISA) and colloidal gold labeling are currently mainly used to achieve quantitative or qualitative detection of samples. These methods have various defects. [0003] Quantum Dot (Quantum Dot, referred to as QD) is a semiconductor nanocrystal developed in the 1990s with excellent spectral characteristics and photochemical stability. It is similar to biomolecules and ha...

AI Technical Summary

Problems solved by technology

The deficiencies of this patent are: (1) It mainly describes the production of quantum dot-labeled immunochromatographic test strips, but does not disclose the corresponding matching detection system. Its detection can only be carried out with the help of other ultraviolet detectors, and it is only a qualitative detection. Quantitative detection cannot be achieved

(2) The use of ultraviolet detection is harmful to the human body and unsafe to operate

(3) The objectivity of the measurement results is poor

Since the tester judges the test result by visually observing the fluorescence depth of the test strip and the quality control zone under ultraviolet light, the result is easily affected by the tester's experience and is highly subjective. It is possible to make different or even wrong judgment results, especially for those samples with extremely low content of the target substance, the fluorescence emitted by the ultraviolet light is very shallow, it is difficult to judge the results with the naked eye, and the sensitivity is low

(4) Cannot monitor the dynamic process of biological reactions

Since immunochromatography is a dynamic reaction process, the fluorescence produced by the quantum dot labeling particles in the siphon migration state on the test strip belongs to the dynamic category. Obviously, the ultraviolet visual method cannot observe and analyze this dynamic reaction process, so it cannot be changed. In-depth study of the immunochromatographic reaction process

Even if the biological reaction fails, the detector cannot analyze the reason for the failure of the reaction well

[0011] Chinese patent application No. 200610024566.3 discloses a method of combining quantum dot fluorescent probes to detect biochips to find Chinese medicine targets. This patent also has shortcomings: the detection of the "quantum dot-Chinese medicine ingredients" fluorescent probes involved not only requires other technologies to provide Corresponding protein chips, and its detection also requires additional special equipment

[0012] Chinese patent application number 200510059892.3 discloses an optoelectronic rapid diagnostic test system including quantum dot test strip detection, but this patent has not yet developed a ready-made product, and the content is completely different from the present invention

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