Quantitative detection system of quantum dot mark test strip based on CMOS image sensor

Abstract

The invention belongs to the field of biomedical medical apparatuses, particularly relating to a quantitative detection system of quantum dot mark test strip based on a CMOS image sensor and a method thereof. The system comprises a quantum dot mark test strip, a test strip bracket, a scanning system, a CMOS image sensor, a data processing device, an output displaying device, a printer, a keyboard and an IC card matched with the test strip, wherein, after reading an IC card parameter, the data processing device controls the motion of the test strip bracket so as to adapt to that the fiber optic probe of the scanning system automatically scans a test strip detecting zone and a quality control zone; the collected characteristic wavelength reflection fluorescence signal is transmitted to the data processing device by the CMOS image sensor for identifying optical density and calculating concentration, and the output displaying device displays the testing result. The invention can quickly and accurately realize quantitative or qualitative detection of single-component samples and multi-component samples and can dynamically monitor an immunity chromatography reaction process. The system of the invention has the characteristics of high detection sensitivity, object result, flexible utilization and the like.

Description

technical field [0001] The invention belongs to the field of biomedical devices, in particular to a CMOS image sensor-based quantitative detection system for quantum dot labeling test strips and a method thereof. The system can scan, detect and interpret quantum dot-labeled test strips to realize biological detection of pathogens, antigens, antibodies, prohibited drugs, major diseases (tumor, cancer, cardiovascular disease, diabetes, etc.), pesticide residues, and food safety. Quantitative or qualitative detection of various target objects. Background technique [0002] In immunoassay, methods such as radioimmunoassay, enzyme-linked immunosorbent assay (ELISA) and colloidal gold labeling are currently mainly used to achieve quantitative or qualitative detection of samples. These methods have various defects. [0003] Quantum Dot (Quantum Dot, referred to as QD) is a semiconductor nanocrystal developed in the 1990s with excellent spectral characteristics and photochemical s...

AI Technical Summary

Problems solved by technology

The disadvantages are: (1) The patent application number only describes the production of quantum dot marking test strips, but does not disclose the corresponding matching detection system. The detection can only be carried out with the help of other ultraviolet detectors, and it is only a qualitative detection. Quantitative detection cannot be achieved

(2) The use of ultraviolet detection is harmful to the human body and unsafe to operate

(3) The objectivity of the measurement results is poor

Since the tester judges the test result by observing the fluorescence intensity of the test strip and the quality control zone under ultraviolet light with the naked eye, the test result is easily affected by the tester's experience and is highly subjective. The same test strip is in different states. It is possible to make different or even wrong judgment results, especially for those samples with extremely low target content, the fluorescence emitted by ultraviolet light is very weak, it is difficult to judge the results with the naked eye, and the sensitivity is low

(4) Cannot monitor the dynamic process of biological reactions

Immunochromatography is a dynamic reaction process. The fluorescence produced by the quantum dot label particles in the siphon migration state on the test strip belongs to the dynamic category. Obviously, the ultraviolet visual method cannot observe and analyze this dynamic reaction process, so it is impossible to go deeper. To study the immunochromatographic reaction process

Even if the biological reaction fails, the detector cannot analyze the reason for the failure of the reaction well

[0011] Chinese patent application No. 200610024566.3 discloses a method of combining quantum dot fluorescent probes to detect biochips to find Chinese medicine targets. This patent also has shortcomings: the detection of the "quantum dot-Chinese medicine ingredients" fluorescent probes involved not only requires other technologies to provide Corresponding protein chips, and its detection also requires additional special equipment

[0012] Chinese patent application number 200510059892.3 discloses an optoelectronic rapid diagnostic test system including quantum dot test strip detection, but this patent has not yet developed a ready-made product, and the content is completely different from the present invention

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