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Method for identifying aflatoxin B1 and B2 by using terahertz time-domain spectroscopy technology

An aflatoxin and terahertz time-domain technology, which is applied in the field of terahertz time-domain spectroscopy detection, can solve the problems of unextracted aflatoxin powder pure product optical parameters, high detection requirements, and high cost

Active Publication Date: 2019-11-15
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are corresponding detection methods and standards for the detection of aflatoxins in my country. The existing detection methods for aflatoxins include enzyme-linked immunosorbent assay, high performance liquid chromatography, thin layer chromatography, isotope dilution liquid chromatography-tandem Mass spectrometry, etc., but the above methods all have the disadvantages of complex sample pretreatment, long detection time, high cost, and high detection requirements.
At present, the terahertz spectrum detection of aflatoxin is based on the terahertz time-domain spectroscopy (THz-TDS) to detect aflatoxin B1 and M1 solution, and there is no detection and identification of aflatoxin B1 and B2 in solid samples. Related studies have not extracted the optical parameters of pure aflatoxin powder

Method used

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  • Method for identifying aflatoxin B1 and B2 by using terahertz time-domain spectroscopy technology

Examples

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Embodiment 1

[0043] Embodiment 1 Utilizes the method of terahertz time-domain spectroscopy to identify aflatoxin B1 and B2

[0044] This embodiment provides a method for qualitatively identifying aflatoxin B1 and B2 (AFB1 and AFB2) using terahertz time-domain spectroscopy, wherein the samples to be tested are standard samples of aflatoxin B1 and B2 and polyethylene powder at a ratio of 1:20 Prepared by mixing tablets.

[0045] Test environment: The terahertz optical path is filled with nitrogen, and the relative humidity in the box is <10%.

[0046] The procedure for identifying aflatoxins B1 and B2 based on terahertz time-domain spectroscopy provided in this example is shown in figure 1 , the method includes:

[0047] 1. After drying treatment of aflatoxin B1 and B2 and polyethylene powder, use an electronic balance to weigh 8.6 mg of aflatoxin B1 and B2 standard powder and two 172.0 mg of polyethylene powder respectively;

[0048] 2. Stir evenly and put it into the tableting mold. The...

Embodiment 2

[0071] Embodiment 2 Utilizes the example of terahertz time-domain spectroscopy to identify aflatoxin B1 and B2

[0072] Peanuts are one of the crops most susceptible to A. flavus infection. This example provides a method for identifying aflatoxins B1 and B2 in peanut oil using terahertz time-domain spectroscopy. Among them, the "peanut oil sample" to be tested is prepared by adding a known amount of aflatoxin B1 and B2 (Shanghai Yika Biotechnology Co., Ltd.) to peanut oil that does not contain any Aspergillus flavus itself (Luhua brand peanut oil, purchased from a supermarket). And described to-be-tested " peanut oil sample " adopts the method of the present invention to detect as blind sample, concrete steps are as follows:

[0073] (1) Mix 0.5ml of peanut oil with 0.2mg of aflatoxin B1 and B2 respectively, and transfer it to a plastic container with a syringe;

[0074] (2) The plastic container has a plastic-gap-plastic sandwich structure, in which the thickness of the fro...

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Abstract

The invention provides a method for identifying aflatoxin B1 and B2 by using a terahertz time-domain spectroscopy technology. The method comprises the following steps of mixing an AFB1 and AFB2 pure products and a polyethylene powder, tabletting, and acquiring the terahertz spectroscopic data of the tabletting sample in a range of 0.5-2.5THz through a terahertz time-domain spectroscopy system separately; working out the refractive index and the absorption coefficient of the tabletting sample by a flat plate medium model firstly, and then working out refractive index and the dielectric constantof the AFB1 and AFB2 pure products through an effective medium model, and making comparison between the refractive index and the absorption coefficient of the AFB1 and AFB2 tabletting sample and therefractive index and the dielectric constant of the pure products, so as to provide reference basis for identification of AFB1 and AFB2 in to-be-tested samples. By means of nondestructive and non-contact measurement, the precise numerical value of the physical quantities of the macroscopic optical properties of the aflatoxin B1 and B2 can be represented in the terahertz wave band, and an effectivemeans is provided for rapid, nondestructive detection and identification of the samples.

Description

technical field [0001] The invention relates to a terahertz time-domain spectrum detection technology, in particular to a method for identifying aflatoxin B1 and B2 by using the terahertz time-domain spectrum technology. Background technique [0002] Aflatoxins are a class of highly toxic compounds that have been classified as Class I carcinogens by the Cancer Research Institute of the World Health Organization (WHO). Among them, the acute toxicity of aflatoxin B1 is 68 times that of arsenic, and its carcinogenic ability is 75 times that of dimethylnitrosamine. The chemical structure of aflatoxin B2 is similar to that of aflatoxin B1, and its toxicity and carcinogenicity are relatively weak. Therefore, the detection and identification of aflatoxin B1 and B2 is the focus of attention. In order to prevent the contamination of aflatoxin in grain, oil and feed, and endanger the health of the people, it is of great significance to establish an accurate and fast qualitative ident...

Claims

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Application Information

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IPC IPC(8): G01N21/3586G01N21/41G01N27/22
CPCG01N21/3586G01N21/41G01N27/221G01N21/274G01N2201/127
Inventor 杨玉平张成
Owner MINZU UNIVERSITY OF CHINA
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