A method for the identification of aflatoxins b1 and b2 by terahertz time-domain spectroscopy
An aflatoxin and terahertz time-domain technology, applied in the field of terahertz time-domain spectroscopy detection, can solve the problems of unextracted aflatoxin powder pure product optical parameters, long detection time, high detection requirements, etc., to achieve fast Accurate qualitative detection, fast analysis speed, simple preparation effect
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Embodiment 1
[0043] Embodiment 1 Utilizes the method of terahertz time-domain spectroscopy to identify aflatoxin B1 and B2
[0044] This embodiment provides a method for qualitatively identifying aflatoxin B1 and B2 (AFB1 and AFB2) using terahertz time-domain spectroscopy, wherein the samples to be tested are standard samples of aflatoxin B1 and B2 and polyethylene powder at a ratio of 1:20 Prepared by mixing tablets.
[0045] Test environment: The terahertz optical path is filled with nitrogen, and the relative humidity in the box is <10%.
[0046] The procedure for identifying aflatoxins B1 and B2 based on terahertz time-domain spectroscopy provided in this example is shown in figure 1 , the method includes:
[0047] 1. After drying treatment of aflatoxin B1 and B2 and polyethylene powder, use an electronic balance to weigh 8.6 mg of aflatoxin B1 and B2 standard powder and two 172.0 mg of polyethylene powder respectively;
[0048] 2. Stir evenly and put it into the tableting mold. The...
Embodiment 2
[0071] Embodiment 2 Utilizes the example of terahertz time-domain spectroscopy to identify aflatoxin B1 and B2
[0072] Peanuts are one of the crops most susceptible to A. flavus infection. This example provides a method for identifying aflatoxins B1 and B2 in peanut oil using terahertz time-domain spectroscopy. Among them, the "peanut oil sample" to be tested is prepared by adding a known amount of aflatoxin B1 and B2 (Shanghai Yika Biotechnology Co., Ltd.) to peanut oil that does not contain any Aspergillus flavus itself (Luhua brand peanut oil, purchased from a supermarket). And described to-be-tested " peanut oil sample " adopts the method of the present invention to detect as blind sample, concrete steps are as follows:
[0073] (1) Mix 0.5ml of peanut oil with 0.2mg of aflatoxin B1 and B2 respectively, and transfer it to a plastic container with a syringe;
[0074] (2) The plastic container has a plastic-gap-plastic sandwich structure, in which the thickness of the fro...
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