55 results about "Antibody adsorption" patented technology

The invention discloses a chromatographic medium using amino benzimidazole as a function ligand and a preparation method thereof. Hydrophilic porous microspheres are used as a chromatographic medium, activated by allyl bromide, and coupled with the amino benzimidazole to obtain a medium using the amino benzimidazole as the function ligand; dimethyl sulfoxide and the allyl bromide are sequentially added into a chromatographic matrix for activation; the activated chromatographic matrix is reacted with N-bromo-succinimide for bromo-alcoholization; the bromo-alcoholized chromatographic matrix is mixed with an amino benzimidazole solution for coupling the amino benzimidazole ligand; finally an aqueous ethanol amine solution is used for sealing unreacted bromo-alcoholized ends to obtain a hydrophobic charge induced chromatographic medium using the amino benzimidazole as the function group. The new chromatographic medium is simple in preparation process and high in antibody adsorption capacity, and has the characteristics of non salt dependent adsorption, can realize desorption and recovery by changing the solution pH to weak acid, and can be used for hydrophobic charge induction chromatographic separation of antibodies.

The invention relates to a synthetic method used for preparing an adsorbent for clearing a pathogenic antibody by oxidizing periodate, and discloses a method for preparing a protein immunoadsorption material by oxidizing the periodate by taking agarose gel as a carrier. The method comprises the following steps of: preparing aldehyde group-containing agarose gel by oxidizing the agarose gel by using the periodate; coupling the aldehyde group-containing agarose gel and immunoglobulin binding protein; and blocking a material and reducing or reducing directly without blocking to obtain the immunoadsorption blood purification material. The method has the characteristics of simple process, environmental friendliness, low cost and the like; and simultaneously the product has high specificity, adsorptive property, regenerability and the like, and can be used for clinical immunoadsorption treatment practically.

The invention relates to a magnetic composite nanoparticle with selective adsorption to antibodies. The structural formula of the magnetic composite nanoparticle is shown as the specification. The method includes: taking an Fe3O4 nanoparticle with superparamagnetism as the core, using tetraethyl orthosilicate as the silicon source, and employing SiO2 or a polymer to coat the Fe3O4 magnetic nanoparticle, thus obtaining a core-shell structure Fe3O4SiO2 or Fe3O4 polymer magnetic composite particle; and bonding a silane coupling agent mercaptoethyl trimethoxy silane to the silica gel surface of the magnetic composite particle, and then carrying out mercapto-ene click reaction on product and double bond-containing vinylpyridine (MEP), thus obtaining the Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle adsorbent. The Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle is a novel bio-functional magnetic composite nano-material with selective adsorption to antibodies. The material has very good superparamagnetism, and can rapidly realize solid-liquid separation under the effect of an external magnetic field, also shows specificity to antibody adsorption, and can be used for rapid separation and preparation of antibodies.

The present invention provides a novel aptamer for IgG and a method for utilizing the same and the like. More specifically, the present invention provides an aptamer that binds to an Fc region of IgG (e.g., human IgG); a complex comprising an aptamer and a functional substance bound thereto (e.g., affinity substance, labeling substance, enzyme, drug, toxin, drug delivery vehicle); a solid phase carrier with an aptamer or complex immobilized thereon; medical equipment comprising a solid phase carrier; a method for antibody purification comprising adsorbing an IgG antibody to a solid phase carrier, and eluting the adsorbed IgG antibody with an eluent; a method for producing a purified antibody, comprising preparing an IgG antibody and purifying the prepared IgG antibody with a solid phase carrier and the like.

The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.5 to 1000, thereby separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types of proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionation solution contains an organic solvent.

Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.

The invention discloses a method for identifying sensitivity of an antibody as well as quality of a clone cell strain in the preparation process of the antibody. The method comprises the following steps: acquiring a solid-phase carrier, cells and the antibody, adsorbing the antibody on the solid-phase carrier, incubating the cells and the antibody adsorbed on the solid-phase carrier, keeping cells that are combined with the antibody, dyeing and counting the cells combined with the antibody, and identifying the sensitivity of the antibody and the quality of the clone cell strain according to the cell counting result. The invention further provides a kit applicable to the method. The method intuitively reflects the level of combination of the antibody and a native conformation antigen, and the identifying method is simple, quick and intuitive.

The invention discloses a chromatography medium comprising a substrate coupled with spacer arms and a functional ligand. The functional ligand is a five-substituted benzotriazole, and the structure thereof is as shown in the description, wherein R is carboxyl, amino, sulfydryl or hydroxyl. The chromatography medium is prepared through a simple process and is low in cost, and compared with other types of antibody adsorption medium, the chromatography medium has the advantage of high dynamic adsorption capacity. Target protein is desorbed and recycled under weak acid condition by regulating the pH of a buffer solution. The novel chromatography medium is relatively high in stability when contacting with acid and alkaline solutions and organic solvents, and can suffer from high-temperature sterilization. The chromatography medium can be used for separating or purifying antibodies in a hydrophobic charge-induced chromatography mode, and can be used as an antibody selective absorbent for blood purification treatment of autoimmune diseases.

The invention discloses a chromatographic medium using amino benzimidazole as a function ligand and a preparation method thereof. Hydrophilic porous microspheres are used as a chromatographic medium, activated by allyl bromide, and coupled with the amino benzimidazole to obtain a medium using the amino benzimidazole as the function ligand; dimethyl sulfoxide and the allyl bromide are sequentially added into a chromatographic matrix for activation; the activated chromatographic matrix is reacted with N-bromo-succinimide for bromo-alcoholization; the bromo-alcoholized chromatographic matrix is mixed with an amino benzimidazole solution for coupling the amino benzimidazole ligand; finally an aqueous ethanol amine solution is used for sealing unreacted bromo-alcoholized ends to obtain a hydrophobic charge induced chromatographic medium using the amino benzimidazole as the function group. The new chromatographic medium is simple in preparation process and high in antibody adsorption capacity, and has the characteristics of non salt dependent adsorption, can realize desorption and recovery by changing the solution pH to weak acid, and can be used for hydrophobic charge induction chromatographic separation of antibodies.

This invention relates to a method which is through the antigen mark which is caught by the immune body adsorption surface matter on, and analyzes with the mass spectrum examines. Adsorbs the many kinds of antigen mark in on a many kinds of immune bodies adsorptions surface, and carries on the mass spectrum analysis to the capture antigen mark. May examine many antigen mark group. This method may use examining the antigen mark combination in the biological sample. Examines the measurement with these antigen mark combinations to be possible at the same time to distinguish the many kinds of diseases.This method is accurate, convenience also quickly.

The invention relates to acrylic macromolecular surfactant and a method for making the porous resin of the same, belonging to the organic macromolecular compound field. The acrylic macromolecular surfactant comprises 93 to 97 percent of acrylic substance, 3 to 7 percent of vinyl ion reagent and 0.5 to 1 percent of initiator. The substances are added in a reactor containing an amount of water, and are reacted at a temperature controlled between 57 and 80 DEG C for 6 to 24 hours; then, the reactants undergo acetone deposition, ether scrubbing and deposition and vacuum drying. The porous resin is made through adopting the following steps that: the acrylic macromolecular surfactant, monomer, initiator and a crosslinking agent are added in water, cyclohexane or the mixed solution of water and cyclohexane; and round porous resin is made by means of emulsion polymerization under the action of the initiator. The method makes the porous resin by means of the acrylic macromolecular surfactant, wherein the surfactant has excellent performances and full emulsification; and the porous resin is round in shape with the diameter ranging between 50 and 500 micron. The porous resin made by the method contains active epoxy group, and can be used in the fields of enzyme immobilization, antibody adsorption and purification and blood pathogen or toxin cleaning, etc.

The invention relates to a test strip for testing bovine leukemia antibody by using in vitro expression, separation and purification of cyst membrane glycoprotein gp51 of bovine leukemia virus (BLV), and gold immunochromatography and a preparation method thereof. Prokaryotic expression vector pET-32a-gp51 is constructed and recombined and the expression of GP51 recombined protein is inducted in colibacillus, chromatography purifies the recombined protein to obtain His-gp51 fusion protein, the purified His-gp51 fusion protein and goat-anti-bovine IgG are spotted on a nitrocellulose membrane for respectively being taken as testing line (T line) for capturing BLV antibody and quality control line (C line) for capturing bovine IgG, the goat-anti-bovine IgG (Fc) marked by the colloidal gold isabsorbed to a glassfiber to make into gold pad, so as to assemble the colloidal gold testing strip. Whether the BLV antibody exists in blood serum is determined by whether color belts appear on the testing line and the quality control line.

The invention relates to a maternal fetal blood group incompatibility antibody adsorption therapeutic apparatus in the field of medical science. The therapeutic apparatus is characterized in that Rh positive O-shaped red blood cells are cleaned by 4 DEG C and 37 DEG C normal saline and alternately washed by 25 and 35mmol / L of PB lysate with PH (potential of hydrogen) 7.4, D antigen containing ghost cells without hemoglobin are prepared, 80% cell concentration is formed by the aid of red blood cell storage solution with 3.5% mannitol, the ghost cells are placed in a cylindrical adsorber made of high-biocompatibility materials, sealed and stored at the temperature of 4 DEG C, and a screen is arranged at an outlet of the adsorber to form a defense line for preventing cell fragments from filtration. After plasma is filtered, Rh antibodies are combined into fixed complexes by the Rh positive red blood cells, the broken red blood cell fragments and macromolecular complexes formed by combination are intercepted by the screen of the adsorber, the plasma without morbid substances is filtered from the adsorber and then returned, and group incompatibility is treated by removing the Rh antibodies in the plasma.

The invention relates to a chromatography medium adopting tryptamine as a functional ligand. The chromatography medium comprises a chromatography matrix and a ligand, wherein the chromatography matrixis hydrophilic porous microspheres, and the ligand is trytamine coupled after being activated by bromoacetyl bromide. The chromatography medium is high in antibody adsorption capacity, the saturatedadsorption capacity can reach 140mg / ml of wet medium, and the processing capacity is high; and the medium is stable in characteristics, and the ligand is activated and coupled by adopting the allyl bromide, so that the ligand of the medium is stable, and convenience in cleaning and regeneration can be realized.

The invention relates to an protein A domain Z derivative with a specific binding effect on an antibody and an application thereof. According to the invention, mutation of N23T and mutation of at least one amino acid residue are generated in a domain Z amino acid sequence. The derivative is obtained through a strategy of mutation of at least one amino acid residue in Helix1 and 2 in an protein A mutation domain Z. In addition, the invention also provides a preparation method for an affinity chromatography matrix by utilizing the domain Z derivative as a ligand. Compared with the affinity chromatography matrix by utilizing the domain Z derivative as the ligand, the domain Z derivative provided by the invention has higher chemical stability, and has a protein denaturation temperature in alkaline solution increased by 4 DEG C; and the synthesized affinity chromatography matrix maintains considerable antibody adsorption capacity.

The invention discloses a blood platelet magnetizing and immunolabeling analysis method. The method comprises the following steps: uniformly mixing blood platelets and magnetic beads; magnetizing the blood platelets to obtain suspension; uniformly mixing, incubating and washing the magnetized blood platelets and a detected sample; adding a labeled second antibody and incubating to obtain mixed liquid; applying magnetic force to layer the mixed liquid; determining upper reaction liquid or determining after washing the magnetized blood platelets in the lower layer to obtain a result, namely performing double-phase complementary immune analysis. In the mode, the blood platelet magnetizing and immunolabeling analysis method provided by the invention is used for detecting blood platelet related antigen antibody and cross-matching of blood; the magnetic force is applied so that the magnetized blood platelet is washed without centrifuging; the method is easy and convenient to operate; by a labeled antibody adsorption test for directly determining the upper reaction liquid, the non-specific adsorption in an immunolabeling technology is overcome, the processes of closing and washing are reduced, and the time consumption is short. The method can detect a large number of samples and facilitates automation; the two detection results of the double phases can be verified mutually, so that the accuracy of the detection results is improved.

The invention discloses a detection test paper. The test paper comprises (1) a reaction support, (2) a water absorption pad, (3) a nitrocellulose membrane, (4) a gold-labeled antibody adsorption membrane and (5) a sample pad, wherein the nitrocellulose membrane is coated with a detection strip and a quality control strip of a staphylococcal enterotoxin B antibody and a quality control antibody; and the gold-labeled antibody adsorption membrane contains the staphylococcal enterotoxin B antibody labeled by colloidal gold. The test paper can be matched with a small instrument to carry out semi-quantitative detection, does not need professional training, has clear and identifiable result, can objectively store data, is simple to operate, easy to popularize, suitable for a basic level, suitable for field detection of emergency and suitable for epidemiological investigation, and plays an auxiliary role in diagnosis of staphylococcal enterotoxin B infection.

The invention relates to a human IgG4 (immunoglobulin G4) antibody adsorption column and a preparation method and application thereof. The human IgG4 antibody adsorption column comprises a column body and is characterized in that two ends of the column body are closed, a connection pipe is arranged at each of the two closed end of the column body, an adsorbent is filled in an inner cavity of the column body and consists of a ligand and a carrier, an anti-human IgG4 antibody serves as the ligand, the carrier is selected from agarose microspheres, polystyrene microspheres or magnetic beads, and the column body is in a shape of a cylinder, an elliptical cylinder or a polygonal column. The preparation method includes: sealing one end of the column body, arranging one of the connection pipes at the sealed end, and filling the absorbent into the inner cavity of the column body; sealing the other end of the column body, arranging the other connection pipe at the sealed end to obtain the human IgG4 antibody adsorption column. Purification of human IgG4 from human blood is realized by the aid of the human IgG4 antibody adsorption column, and recycling of the human IgG4 antibody adsorption column is realized. The human IgG4 antibody adsorption column has advantages of high efficiency and specificity in human IgG4 antibody purification and lays a foundation for IgG4 research and application.

A method of capturing multiple virus germ biological label at biological chip absorption surface and carrying out analysis to captured biological label can be used to detect multiple biological label group such as hepatitis B, hepatitis C, Aide and syphilis in blood in order to identify normal person or person with abovesaid disease.

The invention provides a grouping purification method of olfactory ensheathing cells (OECs) of a neonatal rat, and the method is applied to a purification process of the OECs of the neonatal rat, wherein the purification process comprises the following steps of: obtaining the OECs; separating the OECs; performing grouping purification and comparison on the OECs; and performing morphologic observation and OECs purity detection. By virtue of different purification method experiments of the OECs of the neonatal rat, results indicate that the OECs of an in-vitro cultured neonatal rat are mainly bipolar or tripolar cells, and the neurite of the cell is fine. The OECs which are not purified have quickly-growing and dominated fibroblast, and the three purification methods realize that the purity average value of the OECs after being inoculated for 14 days is more than 75 percent. The purification rates of a p75 antibody adsorption method and a cytarabine inhibition method are slightly higher than that of a difference-speed adherence method. Therefore, cell purification in OECs primary culture of the neonatal rat is necessary; and in a spinal cord injury and regeneration research, compared with the p75 antibody adsorption method and the cytarabine inhibition method, the difference-speed adherence method is a simple, economical and practical OECs purification method.

The invention relates to a kit and a novel method of detecting and evaluating the patients with the severe Hepatitis B, and belongs to a non-invasive external detection method. The method utilizes the mass spectrometry to identify and detect a novel variant beta2-microglubolin. The variant beta2-microglubolin or the peak value of 11729 minus and plus 15Da can be used to detect and evaluate the severe Hepatitis B. The rising of the variant beta2-microglubolin or the peak value of 11729 minus and plus 15Da promotes the poor prognosis. The variant beta2-microglubolin level in the serum of the severe Hepatitis B is obviously higher than that of the acute Hepatitis B. The variant beta2-microglubolin level in the serum of people died from the severe Hepatitis B is obviously higher that of the survivor. The method provided by the invention can detect the seriously injured patient, the sensitivity is 100 percent and the specificity is 100 percent. By detecting the peak value of 11729 minus and plus 15Da or the variant beta2-microglubolin captured on the adsorption surface medium of immune body via the quantitative spectrum analysis controlled by the serum of the standardized quality control, the method can be applied in the development of the detection method or the kit for the biological symbol combination in the body fluid separated from the human body. The method has the advantages of accuracy, convenience and rapidness.

The invention provides a double-antibody sandwich ELISA kit for detecting a gosling plague antigen and an application thereof and belongs to the field of bio-technical detection. The kit includes: an ELISA plate coated by an anti-gosling plague virus (anti-GPV) monoclonal antibody, a GPV standard positive antigen, and enzyme-labeled anti-GPV monoclonal antibody aiming to different epitopes of the GPV. The kit is provided according to the following technical principle: ELISA is a technology that a known antigen or antibody is adsorbed onto the surface of a solid-phase carrier so that an enzyme-labeled antigen-antibody reaction is carried out on the solid-phase surface. The kit can be used for test large-molecular antigens or specific antibodies or the like, is quick, sensitive and simple and is easy to standardize.

The invention relates to the field of immunochromatography detection and analysis, in particular to a capacitive-resistive immunological test strip modified by a carbon nanotube complex and a preparation method thereof. The test strip comprises a sample pad sequentially pasted on a nitrocellulose membrane, Antibody adsorption pad and water absorption pad, the nitrocellulose membrane between the antibody absorption pad and the water absorption pad is modified with at least 2 reaction zones based on the reaction lines of carbon nanotube-binder complexes, each reaction line has two Both terminals are provided with graphite electrode pins, and the graphite electrode pins are connected to the signal input end of the external signal gain circuit to measure the capacitance or resistance value of the test strip. The capacitance or resistance value of the detection line is increased after the antigen-antibody complex is formed, so that an external circuit can be used to form a signal amplification and enhance the detection sensitivity. The device of the invention provides a novel immune test strip structure and detection method, and expands the application prospect of immune chromatography detection as a POCT platform.

The present invention addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present invention also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution ata specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method comprising a step of allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, a step of adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and a step of eluting the antibody adsorbed on the carrier using an elution solution.

The invention provides a method for separating newly born rat olfactory bulb olfactory ensheat hing cells (OECs). The method is applied to the purification process of the OECs. The purification process comprises the following steps of: collecting the OECs, separating the OECs, and grouping, purifying and comparing the OECs; and performing morphologic observation and detecting the purity of the OECs. Through experiments of different methods for purifying the newly born rat olfactory bulb OECs, the newly born rat olfactory bulb OECs which are cultivated in vitro are mainly bipolar cells or three-pole cells, and the protuberances of the cells are thin and long. The OECs which are not purified are fibroblast which grows quickly, and is dominant; and the average value of the purity of the OECs which are inoculated after 14 days is more than 75 percent by the three purification methods. The purification rate of a p75 antibody adsorption method and a cytarabine inhibition method is slightly greater than that of a differential adhesion method. The cell purification of the newly born rat olfactory OECs is necessary in primary culture; and in research on spinal cord injury and regeneration, compared with the p75 antibody adsorption method and the cytarabine inhibition method, the differential adhesion method is simple, economic and practical method for purifying the OECs.

The invention relates to a composite material containing graphene oxide and a recombinant streptococcal protein G, and a preparation method and application thereof. According to the invention, by utilizing the characteristic of high specific surface area of the graphene oxide, through a covalent binding method, the recombinant streptococcus protein G is immobilized onto the graphene oxide so as to prepare a high-capacity antibody enrichment material with antibody adsorption biological activity. The prepared composite material containing the graphene oxide and the recombinant streptococcal protein G can be applied in the fields of antibody purification, antibody enrichment, pathogen detection, pretreatment of biological samples, etc.

The invention relates to a kit and a novel method for detecting tumor metastasis and recurrence and evaluating healing effect, which is a detecting method of non-invasive critical protein in vitro. The method finds a novel variant serum amyloid A by identifying and detecting with an antibody to attach the surface matrix and the mass spectrometric method; the variant serum amyloid A or peak values of 11439+-15, 11527+-15 and 11683+-15Da can be used for detections of tumor metastasis and recurrence and the evaluation of healing effect. The higher variant serum amyloid A or peak values of 11439+-15, 11527+-15 and 11683+-15Da prompt that the tumor is transferred and recurred and the healing effect is poor. The method provided by the invention can detect different tumor metastases and recurrences and evaluate the healing effect with 100 percent sensitivity and 100 percent specificity. The invention receives the variant serum amyloid A through a group of peak values of 11439+-15, 11527+-15 and 11683+-15Da or the surface matrix attached by the antibody, detects with quantitative mass spectrum analysis under the control of standard quality control serum, and can be applied to the detection method or kit development of biomarker combination in fluid which is separated from human body. The method is accurate, convenient and quick.

The invention provides an efficient screening method of high-affinity paired antibodies, wherein the method comprises the steps: adsorbing antibodies in positive clone cell supernate through Protein A / G magnetic bead filler, measuring the concentration of the antibodies, selecting a preset amount of antibodies for labeling to form a detection antibody, coating unlabeled antibodies, performing an ELISA test by adopting an orthogonal chessboard method, and screening an experimental group with a high OD value and rechecking antibody pairs according to an ELISA result. According to the formula antibody screening, the whole experiment process from positive clone cell strain determination to antibody pairing is completed only in two days, and the average cost of consumable reagents is less than 100 Yuan, so that the optimal antibody pair can be efficiently obtained at low cost.