55 results about "Antibody adsorption" patented technology

The invention discloses a chromatographic medium using amino benzimidazole as a function ligand and a preparation method thereof. Hydrophilic porous microspheres are used as a chromatographic medium, activated by allyl bromide, and coupled with the amino benzimidazole to obtain a medium using the amino benzimidazole as the function ligand; dimethyl sulfoxide and the allyl bromide are sequentially added into a chromatographic matrix for activation; the activated chromatographic matrix is reacted with N-bromo-succinimide for bromo-alcoholization; the bromo-alcoholized chromatographic matrix is mixed with an amino benzimidazole solution for coupling the amino benzimidazole ligand; finally an aqueous ethanol amine solution is used for sealing unreacted bromo-alcoholized ends to obtain a hydrophobic charge induced chromatographic medium using the amino benzimidazole as the function group. The new chromatographic medium is simple in preparation process and high in antibody adsorption capacity, and has the characteristics of non salt dependent adsorption, can realize desorption and recovery by changing the solution pH to weak acid, and can be used for hydrophobic charge induction chromatographic separation of antibodies.

The invention relates to a magnetic composite nanoparticle with selective adsorption to antibodies. The structural formula of the magnetic composite nanoparticle is shown as the specification. The method includes: taking an Fe3O4 nanoparticle with superparamagnetism as the core, using tetraethyl orthosilicate as the silicon source, and employing SiO2 or a polymer to coat the Fe3O4 magnetic nanoparticle, thus obtaining a core-shell structure Fe3O4SiO2 or Fe3O4 polymer magnetic composite particle; and bonding a silane coupling agent mercaptoethyl trimethoxy silane to the silica gel surface of the magnetic composite particle, and then carrying out mercapto-ene click reaction on product and double bond-containing vinylpyridine (MEP), thus obtaining the Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle adsorbent. The Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle is a novel bio-functional magnetic composite nano-material with selective adsorption to antibodies. The material has very good superparamagnetism, and can rapidly realize solid-liquid separation under the effect of an external magnetic field, also shows specificity to antibody adsorption, and can be used for rapid separation and preparation of antibodies.

The present invention provides a novel aptamer for IgG and a method for utilizing the same and the like. More specifically, the present invention provides an aptamer that binds to an Fc region of IgG (e.g., human IgG); a complex comprising an aptamer and a functional substance bound thereto (e.g., affinity substance, labeling substance, enzyme, drug, toxin, drug delivery vehicle); a solid phase carrier with an aptamer or complex immobilized thereon; medical equipment comprising a solid phase carrier; a method for antibody purification comprising adsorbing an IgG antibody to a solid phase carrier, and eluting the adsorbed IgG antibody with an eluent; a method for producing a purified antibody, comprising preparing an IgG antibody and purifying the prepared IgG antibody with a solid phase carrier and the like.

The invention discloses a method for identifying sensitivity of an antibody as well as quality of a clone cell strain in the preparation process of the antibody. The method comprises the following steps: acquiring a solid-phase carrier, cells and the antibody, adsorbing the antibody on the solid-phase carrier, incubating the cells and the antibody adsorbed on the solid-phase carrier, keeping cells that are combined with the antibody, dyeing and counting the cells combined with the antibody, and identifying the sensitivity of the antibody and the quality of the clone cell strain according to the cell counting result. The invention further provides a kit applicable to the method. The method intuitively reflects the level of combination of the antibody and a native conformation antigen, and the identifying method is simple, quick and intuitive.

The invention discloses a chromatographic medium using amino benzimidazole as a function ligand and a preparation method thereof. Hydrophilic porous microspheres are used as a chromatographic medium, activated by allyl bromide, and coupled with the amino benzimidazole to obtain a medium using the amino benzimidazole as the function ligand; dimethyl sulfoxide and the allyl bromide are sequentially added into a chromatographic matrix for activation; the activated chromatographic matrix is reacted with N-bromo-succinimide for bromo-alcoholization; the bromo-alcoholized chromatographic matrix is mixed with an amino benzimidazole solution for coupling the amino benzimidazole ligand; finally an aqueous ethanol amine solution is used for sealing unreacted bromo-alcoholized ends to obtain a hydrophobic charge induced chromatographic medium using the amino benzimidazole as the function group. The new chromatographic medium is simple in preparation process and high in antibody adsorption capacity, and has the characteristics of non salt dependent adsorption, can realize desorption and recovery by changing the solution pH to weak acid, and can be used for hydrophobic charge induction chromatographic separation of antibodies.

The invention relates to a synthetic method used for preparing an adsorbent for clearing a pathogenic antibody by oxidizing periodate, and discloses a method for preparing a protein immunoadsorption material by oxidizing the periodate by taking agarose gel as a carrier. The method comprises the following steps of: preparing aldehyde group-containing agarose gel by oxidizing the agarose gel by using the periodate; coupling the aldehyde group-containing agarose gel and immunoglobulin binding protein; and blocking a material and reducing or reducing directly without blocking to obtain the immunoadsorption blood purification material. The method has the characteristics of simple process, environmental friendliness, low cost and the like; and simultaneously the product has high specificity, adsorptive property, regenerability and the like, and can be used for clinical immunoadsorption treatment practically.

The invention discloses a blood platelet magnetizing and immunolabeling analysis method. The method comprises the following steps: uniformly mixing blood platelets and magnetic beads; magnetizing the blood platelets to obtain suspension; uniformly mixing, incubating and washing the magnetized blood platelets and a detected sample; adding a labeled second antibody and incubating to obtain mixed liquid; applying magnetic force to layer the mixed liquid; determining upper reaction liquid or determining after washing the magnetized blood platelets in the lower layer to obtain a result, namely performing double-phase complementary immune analysis. In the mode, the blood platelet magnetizing and immunolabeling analysis method provided by the invention is used for detecting blood platelet related antigen antibody and cross-matching of blood; the magnetic force is applied so that the magnetized blood platelet is washed without centrifuging; the method is easy and convenient to operate; by a labeled antibody adsorption test for directly determining the upper reaction liquid, the non-specific adsorption in an immunolabeling technology is overcome, the processes of closing and washing are reduced, and the time consumption is short. The method can detect a large number of samples and facilitates automation; the two detection results of the double phases can be verified mutually, so that the accuracy of the detection results is improved.

The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.5 to 1000, thereby separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types of proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionation solution contains an organic solvent.

The invention relates to a kit and a novel method of detecting and evaluating the patients with the severe Hepatitis B, and belongs to a non-invasive external detection method. The method utilizes the mass spectrometry to identify and detect a novel variant beta2-microglubolin. The variant beta2-microglubolin or the peak value of 11729 minus and plus 15Da can be used to detect and evaluate the severe Hepatitis B. The rising of the variant beta2-microglubolin or the peak value of 11729 minus and plus 15Da promotes the poor prognosis. The variant beta2-microglubolin level in the serum of the severe Hepatitis B is obviously higher than that of the acute Hepatitis B. The variant beta2-microglubolin level in the serum of people died from the severe Hepatitis B is obviously higher that of the survivor. The method provided by the invention can detect the seriously injured patient, the sensitivity is 100 percent and the specificity is 100 percent. By detecting the peak value of 11729 minus and plus 15Da or the variant beta2-microglubolin captured on the adsorption surface medium of immune body via the quantitative spectrum analysis controlled by the serum of the standardized quality control, the method can be applied in the development of the detection method or the kit for the biological symbol combination in the body fluid separated from the human body. The method has the advantages of accuracy, convenience and rapidness.

The present invention addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present invention also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution ata specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method comprising a step of allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, a step of adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and a step of eluting the antibody adsorbed on the carrier using an elution solution.