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Carbon nanotube composite modified capacitance resistance type immune test strip and preparation method thereof

A technology of carbon nanotubes, capacitance and resistance, applied in the field of immunochromatography detection and analysis, can solve the problems of great influence of electrode signal interference substances, inaccurate results, inaccurate measurement, etc., achieve high electrochemical integration, simple operation, Avoid mutual interference effects

Inactive Publication Date: 2018-03-06
BIOHIT BIOTECH HEFEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of these electrochemical detection immunological test strips is too complicated, and the electrode signal is greatly affected by the interfering substances in the sample, and there are problems of inaccurate results and inaccurate measurement.

Method used

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  • Carbon nanotube composite modified capacitance resistance type immune test strip and preparation method thereof
  • Carbon nanotube composite modified capacitance resistance type immune test strip and preparation method thereof
  • Carbon nanotube composite modified capacitance resistance type immune test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Preparation of carbon nanotube-binder composite printing solution

[0043] Carboxylated multi-walled carbon nanotube suspension formation: Accurately weigh 50 mg of multi-walled carbon nanotubes, add sulfuric acid / nitric acid mixed strong acid 100ml (V / V=2:1), shake and react in an ultrasonic oscillator for 2 hours, Take out after cooling.

[0044] Slowly add the above-mentioned mixed acid solution into a beaker of 1000ml deionized water, mix well, let it cool down, and let stand overnight.

[0045]In the above acidic solution, the carboxylated multi-walled carbon nanotubes can gradually precipitate at the bottom of the beaker, gently discard the supernatant strong acidic liquid, then collect the carboxylated carbon nanotube dispersion at the bottom into a centrifuge tube, add 50ml of deionized water, centrifugal washing. Purified carboxylated carbon nanotubes can be obtained after multiple repetitions.

[0046] Accurately weigh 5 mg of the above-mention...

Embodiment 2

[0047] Embodiment 2 Preparation of antibody-modified carbon nanotube-binder composite printing solution

[0048] Accurately weigh 5 mg of the above-mentioned carboxylated carbon nanotubes and disperse them in 5 ml of MES buffer, add 0.2 g of EDC and NHS in turn, centrifuge to remove the supernatant, then add 2 micrograms of the corresponding antibody after washing, and then centrifuge and wash several times. The corresponding antibody-coupled multi-walled carbon nanotube complexes were obtained.

[0049] Further adding the multi-walled carbon nanotube complex to 1 ml of 0.5-2% chitosan solution, the antibody-coupled carboxylated multi-walled carbon nanotube-chitosan complex printing solution can be obtained.

Embodiment 3

[0050] Example 3 Detection of gastric function index gastrin 17 by immunoassay strips

[0051] Device structure:

[0052] Such as figure 1 with 2 As shown, a capacitive-resistance type immunochromatographic detection test strip includes a sample pad 2, an antibody adsorption pad 3 and a water-absorbing pad 4 that are sequentially pasted on a nitrocellulose membrane 1, and an internal standard is adsorbed in the antibody adsorption pad 3. Antibody, on the nitrocellulose membrane 1 between the antibody adsorption pad 3 and the water-absorbent pad 4, there are reaction areas formed by a blank line 5, a measurement line 6, and a quality control line 7 in sequence, each reaction line is sequentially adsorbed with or without antibody or antigen modification. Carbon nanotube-binder complexes, antibody- or antigen-modified carbon nanotube-binder complexes that capture target analytes, anti-antibody-modified carbon nanotube-binder complexes that capture excess internal standard antib...

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Abstract

The invention relates to the field of immunochromatography detection and analysis, in particular to a capacitive-resistive immunological test strip modified by a carbon nanotube complex and a preparation method thereof. The test strip comprises a sample pad sequentially pasted on a nitrocellulose membrane, Antibody adsorption pad and water absorption pad, the nitrocellulose membrane between the antibody absorption pad and the water absorption pad is modified with at least 2 reaction zones based on the reaction lines of carbon nanotube-binder complexes, each reaction line has two Both terminals are provided with graphite electrode pins, and the graphite electrode pins are connected to the signal input end of the external signal gain circuit to measure the capacitance or resistance value of the test strip. The capacitance or resistance value of the detection line is increased after the antigen-antibody complex is formed, so that an external circuit can be used to form a signal amplification and enhance the detection sensitivity. The device of the invention provides a novel immune test strip structure and detection method, and expands the application prospect of immune chromatography detection as a POCT platform.

Description

technical field [0001] The invention relates to the field of immunochromatographic detection and analysis, in particular to a capacitive resistance type immunological test strip modified by a carbon nanotube complex and a preparation method thereof. Background technique [0002] On-site rapid testing (Point Of Care Testing, POCT) is an emerging subdivision of in vitro diagnostics (IVD), which refers to clinical testing performed next to patients, usually not necessarily performed by clinical laboratory technicians. It is a new method for immediate analysis at the sampling site, omitting the complicated processing procedures of the laboratory examiner, and quickly obtaining the test results. POCT is "patient-centered" and is the best carrier to realize personalized service for patients. With the advantages of simplicity, speed and low cost, it has been widely used in clinical practice, and it is an important development direction and the fastest growing field in the IVD indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/543G01N33/558
CPCG01N27/3278G01N33/54306G01N33/558G01N2333/46
Inventor 张宜明刘峰谢龙生
Owner BIOHIT BIOTECH HEFEI
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