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Magnetic composite nanoparticle and preparation method thereof

A magnetic composite nanometer and magnetic nanoparticle technology, applied in chemical instruments and methods, other chemical processes, alkali metal compounds, etc., can solve problems such as difficult purification of antibody proteins, and achieve mild antibody purification methods and rapid separation and purification methods Effect

Inactive Publication Date: 2015-12-23
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is usually difficult to obtain high-purity antibody protein in one step only by ion exchange or hydrophobic chromatography

Method used

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  • Magnetic composite nanoparticle and preparation method thereof
  • Magnetic composite nanoparticle and preparation method thereof
  • Magnetic composite nanoparticle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Fe 3 o 4 Preparation of nanoparticles

[0042] Fe with uniform size and good dispersion was prepared by hydrothermal method 3 o 4 Magnetic Nanomaterials. The general process: add an appropriate amount of FeCl to a dry and clean three-necked bottle 3 ·6H 2 O, then measure ethylene glycol and add it to a three-necked bottle as a solvent, stir mechanically until the solution is yellow and transparent, add 4.0g PEG1000, 14.4g anhydrous sodium acetate, and stir mechanically to dissolve it. Then the above solution was transferred to six 50mL Teflon-lined stainless steel reaction kettles, packaged and placed in a programmable temperature control box at 200°C for 12h, and then naturally cooled to room temperature. Pour out the magnetic fluid obtained after cooling, wash several times with ethanol after magnetic separation (magnetic separation), then wash several times with water three times (magnetic separation), and finally wash several times with absolute ethanol, a...

Embodiment 2

[0050] Preparation of Adsorption Media for Antibody Separation Using 3-Vinylpyridine as Ligand

[0051] with Fe 3 o 4 @SiO 2 Magnetic composite microspheres are used as the matrix, and magnetic nanoparticles with optimal particle size are selected and modified (magnetic composite microspheres of about 200nm are selected), and Fe, which has specific adsorption effect on antibodies, is prepared with MEP as the ligand. 3 o 4 @SiO 2 -MEP magnetic composite microspheres, the preparation process is as follows: First, weigh an appropriate amount of silica magnetic microspheres into a dry 100mL three-necked bottle, add 50mL of anhydrous toluene to it, and add 0.1g of 4-mercaptoethyltrimethoxy base silane, heated to 90°C, and reacted under reflux for 24h. After the reaction was completed, it was washed three times with methanol and water respectively, and then dried in an oven at 50°C for use. Then put the above product in a 100mL three-necked flask, add 50mL of anhydrous toluene...

Embodiment 3

[0054] Preparation of Adsorption Media for Antibody Separation Using 2-Vinylpyridine as Ligand

[0055] with Fe 3 o 4 @SiO 2 Magnetic composite microspheres are used as the matrix, and magnetic nanoparticles with optimal particle size are selected and modified (magnetic composite microspheres of about 200nm are selected), and Fe, which has specific adsorption effect on antibodies, is prepared with MEP as the ligand. 3 o 4 @SiO 2 -MEP magnetic composite microspheres, the preparation process is as follows: First, weigh an appropriate amount of silica magnetic microspheres into a dry 100mL three-necked bottle, add 50mL of anhydrous toluene to it, and add 0.1g of 4-mercaptoethyltrimethoxy base silane, heated to 90°C, and reacted under reflux for 24h. After the reaction was completed, it was washed three times with methanol and water respectively, and then dried in an oven at 50°C for use. Then put the above product in a 100mL three-necked flask, add 50mL of anhydrous toluene...

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Abstract

The invention relates to a magnetic composite nanoparticle with selective adsorption to antibodies. The structural formula of the magnetic composite nanoparticle is shown as the specification. The method includes: taking an Fe3O4 nanoparticle with superparamagnetism as the core, using tetraethyl orthosilicate as the silicon source, and employing SiO2 or a polymer to coat the Fe3O4 magnetic nanoparticle, thus obtaining a core-shell structure Fe3O4SiO2 or Fe3O4 polymer magnetic composite particle; and bonding a silane coupling agent mercaptoethyl trimethoxy silane to the silica gel surface of the magnetic composite particle, and then carrying out mercapto-ene click reaction on product and double bond-containing vinylpyridine (MEP), thus obtaining the Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle adsorbent. The Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle is a novel bio-functional magnetic composite nano-material with selective adsorption to antibodies. The material has very good superparamagnetism, and can rapidly realize solid-liquid separation under the effect of an external magnetic field, also shows specificity to antibody adsorption, and can be used for rapid separation and preparation of antibodies.

Description

technical field [0001] The invention relates to a magnetic composite nanoparticle and a preparation method thereof, in particular to a composite magnetic nanoparticle which uses vinylpyridine as a ligand to selectively adsorb antibodies and a preparation method thereof. Background technique [0002] Antibody engineering technology has been gradually improved with the development of modern biotechnology, and has become the main force in the industrialization of biotechnology, especially in the field of biotechnology and pharmaceuticals. Antibody drugs have been widely used in clinical diagnosis and treatment because of their highly specific curative effect and low side effects on the human body, mainly for improving human immunity and treating malignant tumors. Due to different uses, the sources of antibodies are also very different. For example, the antibodies used to improve human immunity are derived from the plasma of healthy people; the antibodies used to treat malignant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30
Inventor 白泉倪倩
Owner NORTHWEST UNIV
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