89results about How to "The separation and purification method is simple" patented technology

The invention relates to a synthesis method of nucleoside analogs, particularly to a synthesis method of an anti-virus-activity compound Entecavir. The invention also relates to intermediates for preparing Entecavir and a method for preparing the intermediates.

The invention relates to a synthesis method of nucleoside analogs, particularly to a synthesis method of an anti-virus-activity compound Entecavir. The invention also relates to intermediates for preparing Entecavir and a method for preparing the intermediates.

The invention relates to a synthesis method of nucleoside analogues, in particular to a synthesis method of a compound Entecavir with antiviral activity. The invention also relates to intermediates used for preparing Entecavir and methods for preparing the intermediates.

The invention relates to a synthesis method of a nucleoside analogue, in particular to a synthesis method of compound entecavir with antiviral activity. The invention also relates to midbodies for preparing the entecavir and a method for preparing the midbodies.

The invention relates to a magnetic composite nanoparticle with selective adsorption to antibodies. The structural formula of the magnetic composite nanoparticle is shown as the specification. The method includes: taking an Fe3O4 nanoparticle with superparamagnetism as the core, using tetraethyl orthosilicate as the silicon source, and employing SiO2 or a polymer to coat the Fe3O4 magnetic nanoparticle, thus obtaining a core-shell structure Fe3O4SiO2 or Fe3O4 polymer magnetic composite particle; and bonding a silane coupling agent mercaptoethyl trimethoxy silane to the silica gel surface of the magnetic composite particle, and then carrying out mercapto-ene click reaction on product and double bond-containing vinylpyridine (MEP), thus obtaining the Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle adsorbent. The Fe3O4SiO2-S-MEP or Fe3O4 polymer-S-MEP magnetic composite nanoparticle is a novel bio-functional magnetic composite nano-material with selective adsorption to antibodies. The material has very good superparamagnetism, and can rapidly realize solid-liquid separation under the effect of an external magnetic field, also shows specificity to antibody adsorption, and can be used for rapid separation and preparation of antibodies.

The invention belongs to the field of the extraction of natural medicines and particularly relates to a limonin analogue and a separation and purification method thereof. The invention aims to solve the technical problem of providing a fire-new separation and purification method of a limonin analogue. According to the separation and purification method disclosed by the invention, the medicinal materials of rue family citrus plants, such as tangerine seeds, pummelo seeds, immature bitter oranges, tangerine peels and the like, are used as raw materials, 40 to 50 percent of ethanol is used as a solvent for extraction, an extract is separated and purified by adopting HPD-722 type macroporous resin, the content of the limonin analogue in obtained solid matters is greater than 80 percent, the content of limonin is greater than 20 percent, and the content of nomilin is greater than 40 percent. The method has high extraction efficiency, little reagent consumption and little pollution and is simple to operate.

A method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc. The invention relates to a method for separating purified seabuckthorn flvonoid from large berry seabuckthorn marc. The invention solves the problems in the existing separation and purification method of seabuckthorn flavonoid, such as tedious method, complicated techniques, high cost of separation and purification, easy damage to the activity of flavonoid and low purity of flavonoid. The method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc disclosed by the invention comprises the following steps: first, degreasing the raw materials; second, ultrasonic wave extracting and centrifugating; third, adding metal salt to extracting solution to lead complex reaction between flavonoid and metal, agitating, tempering the pH of solution and collecting sediment; and fourth, adding decomplexing additive to resolve sediment, concentrating in a decompressing way to dryness, using ethanol to dissolve and filter, concentrating filtrate, and obtaining the separated and purified seabuckthorn flavonoid powder after drying. The method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc disclosed by the invention can ensure the full improvement of the purity of the purified seabuckthorn flavonoid, and maintains the original biological activity of the natural flavonoid, the seabuckthorn flavonoi is safe and nontoxic, and the method disclosed by the invention has the advantages of low production cost and simple process, and is suitable for the large-scale production of the seabuckthorn flavonoid.

The invention relates to the technical field of detection materials, in particular to a near-infrared bio-thiol fluorescent probe as well as a preparation method and an application thereof. The structural formula of the near-infrared bio-thiol fluorescent probe is shown in the description. The preparation method of the near-infrared bio-thiol fluorescent probe comprises the following step: the target compound is prepared from a compound A and 2,4-dinitrobenzenesulfonyl chloride by a reaction, wherein the structural formula of the compound A is shown in the description. The compound almost hasno fluorescence but emits intense fluorescence after a reaction with bio-thiol, the fluorescence emission wavelength is larger than 650 nm and is in the near-infrared region, and the fluorescence canbe distinguished from autofluorescence of body tissue and can effectively penetrate the tissue to eliminate biological background interference. The compound can be used for detecting bio-thiol, is simple to operate, has fast and sensitive double-response and has broad application prospects in the fields of biomolecular detection and environment.

The present invention relates to anti-cancer active component of Huaiqing chrysanthemum and its separation and purification method. Its anti-cancer compound includes 9 beta-acetyl-costunolide (CP-C) whose molecular formula is C17H22O4 and 9 beta-acetyl-parthenclide (CP-I) whose molecular formula is C17H22O5. Said invention also provides the concrete steps for separation and purification of said anti-cancer components, and discloses three anti-cancer active components of CP-B, CP-C and CP-D.

The invention relates to a chemical synthetic method of a lipopeptide molecule, and especially relates to a lipopeptide molecule surfactant containing an azobenzene photosensitive group, and a synthetic method thereof. The lipopeptide with small molecular weight is synthesized through a liquid phase synthesis process, contains a small number of amino acids and can be easily synthesized through few reaction steps, and the synthesis efficiency and the purity of the lipopeptide with small molecular weight are high; the yield of every step in a synthesis route is high, and a separation and purification process is simple, so the consumption of a raw material and the waste of a product are reduced; and the azobenzene photosensitive group is effectively embedded into a fatty acid chain through the liquid phase synthesis process in order to bind a specific function group.

The invention belongs to the technical field of tobaccos, and particularly relates to a method for separating and purifying neophytadiene in flue-cured tobacco leaves. The method comprises the following steps: carrying out supercritical CO2 extraction on flue-cured tobacco leaves to obtain neophytadiene primary extract, mixing the neophytadiene primary extract with an alkaline solution to obtain an alkaline extracting solution, carrying out back extraction on the alkaline extracting solution to obtain an oil phase, and removing a back extraction agent in the oil phase to obtain high-purity neophytadiene, wherein the back extraction agent for back extraction is a mixture of petroleum ether and alkane or petroleum ether. The method provided by the invention is simple and easy to operate, andthe result of the embodiment shows that the yield of the neophytadiene obtained by the method provided by the invention reaches 15.74% and the purity reaches 97.99%.

The invention relates to a preparation method of esamin phenol. Sesamolin reacts in a benzene series solvent under the action of a catalyzer at the temperature of 70-110 DEG C for 1-6h, and sesamin phenol is obtained after a reaction solution is treated, wherein the catalyzer is an H-type strongly acidic styrene which is cation exchange resin. Compared with the traditional methods, the preparationmethod has the advantages of available raw material, stable conversion process, high sesamin phenol yield, simple separation and purification, green process and the like.

The invention discloses a method for improving foaming property and foam stability of protein. According to the method, high-temperature heating is performed on whey protein concentrate to obtain a nano-fiber polymer, wherein the foaming property and the foam stability are obviously improved. The prepared nano-fiber polymer as an inducer and the whey protein concentrate are mixed and then subjected to heat treatment, so that the foaming property and the foam stability of the whey protein concentrate can be further improved. The whey protein concentrate fiber polymer is hydrolyzed with enzymes,the obtained hydrolysate as the inducer and the whey protein concentrate are mixed and then subjected to high-temperature heating treatment, and results show that the foaming capacity of the treatedwhey protein is improved by 47.73-59.09%, and the foam stability is improved by 84.98-101.1%. The method is low in production cost, the raw materials are broad in source, complex equipment is not needed, the technology is simple, and the method is easy to popularize and apply.

The invention relates to a high-purity arsenic preparation method. The high-purity arsenic preparation method adopts an arsenic crude product as a raw material and orderly comprises 1, an oxidation reaction, 2, an esterification reaction, 3, solid-liquid separation, 4, adsorption purification, 5, solid-liquid separation, 6, rectification purification, 7, hydrolysis-crystallization, 8, solid-liquid separation, 9, water washing, 10, dehydration, 11, drying, and 12, a reduction reaction. The high-purity arsenic preparation method has the advantages of short process, simple separation purification processes, high product purity, stable quality, safe and reliable processes, low energy consumption and less three wastes and is a clean production method satisfying green chemical development requirements.

The invention discloses a novel method for separating and preparing salidroside from glossy privet fruit, which comprises the following steps: heating the medicinal material glossy privet fruit with ethanol under reflux to obtain an extracting solution; recycling the extracting solution under reduced pressure until no alcohol smell is emitted; centrifugalizing, carrying out alkaline hydrolysis on the supernatant, adding acid to regulate the solution to neutral state, carrying out primary separation with macroporous adsorbent resin, and separating by silica gel column chromatography to obtain a crude salidroside product; and purifying the crude product by a solvent method or column chromatography to obtain the white acicular crystals of salidroside. The determination on the salidroside by high efficiency liquid chromatography indicates that the purity is higher than 98%.

The invention relates to vibrio alginolyticus A-001 and a method for preparing agaro-oligosaccharide with agarase generated by the vibrio alginolyticus A-001. The vibrio alginolyticus A-001 is preserved in CCTCC (China Center for Type Culture Collection) in September 18, 2015, and a preservation number of the vibrio alginolyticus A-001 is CCTCC M2015555. The method includes: taking strains for culture, using a fermentation medium composed of a carbon source, a nitrogen source and inorganic salt to generate enzymes, salting out fermentation liquor through ammonium sulfate, centrifuging, dialyzing, passing through iron exchange columns and gel columns to obtain dry enzyme powder, allowing reaction between agarase and 0.5% agar substrate, filtering through an ultrafiltration membrane, collecting filtrate and performing freeze drying to obtain the agaro-oligosaccharide.

The invention relates to a synthesis method of 4-aminoquinaldine. The invention discloses the synthesis method of the 4-aminoquinaldine; the method comprises the following steps: enabling 2-methyl quinoline, taken as a starting raw material, to be subjected to a hydrogen peroxide oxidation reaction in acetic acid so as to generate 2-methylquinoline N-oxide; then, carrying out a nitration reactionto generate 2-methyl-4-nitroquinoline N-oxide; after that, illuminating for carrying out a reaction to generate 2-methyl-4-nitroquinoline; producing 2-methyl-4-aminoquinoline N-oxide by means of interaction of ferric trichloride hexahydrate and hydrazine hydrate in ethanol; finally, carrying out a reduction reaction by using iron powder under the action of acetic acid so as to generate the 4-aminoquinaldine. The synthesis method provided by the invention has the beneficial effects that the used raw materials and reagents are low in price and easy to obtain, the synthesis method is simple and feasible, the reaction conditions are mild, and a simple and convenient synthetic route is opened up to the synthesis of the 4-aminoquinaldine.

The invention discloses a preparation method of a porcine TLR4 polyclonal antibody. According to the preparation method, recombinant TLR4 protein (antigen) and freund's adjuvant are emulsified, then an immune animal obtains polyclonal antibody serum, wherein the recombinant TLR4 protein has the amino acid sequence as indicated in the sequence table SEQ. ID. No. 1. It is shown through tests that the antibody prepared through the method is good in reactivity and specificity; due to the fact that a TLR4 gene is a member of a significant pattern recognition receptor Toll-like receptor family targeted to innate immune response, the porcine TLR4 polyclonal antibody can be applied to detection of the porcine TLR4 protein and related research; a good foundation is laid for researching the interaction mechanism between porcine bacterial and viral infection diseases and TLR4 and the virus immunity escape mechanism, and a new target is provided for developing new generation vaccine and medicine. Meanwhile, a prokaryotic expression vector constructed through the method is high in recombinant protein expression efficiency, and the separation and purification method is simple and easy to operate.

The invention relates to a separation and refining method of arsenic trioxide. According to the method, a arsenic trioxide crude product is used as raw material and undergoes the following steps: (1) esterification reaction; (2) solid-liquid separation; (3) adsorption and impurity removal; (4) solid-liquid separation; (5) rectification and impurity removal; (6) hydrolysis-crystallization; (7) solid-liquid separation; (8) washing; (9) dehydration; and (10) drying. Thus, high-purity arsenic trioxide is obtained. The separation and refining method has the following advantages: process flow is short; the separation and refining method is simple; product purity is high; quality is stable; the process is safe and reliable; energy consumption is low; the amount of ''three wastes (waste gas, waste water and industrial residue)'' is small; and the separation and refining method is a clean production method which is adopted for the preparation of high-purity arsenic trioxide and accords with development requirements of the green chemical industry.

The invention provides a method for processing a plant seed mixture, and belongs to the field of seed processing. After a seed mixture and sand are mixed, vibrated and rubbed, seeds are separated from pectin, and further filtering, separation and washing are performed to obtain seeds. On the basis of a physical mixing vibration method, chemical treatment and fermentation methods in the prior art are avoided, and the seed yield and the seed quality are improved.

The invention provides a preparation method for a polyclonal antibody against chicken caspase-1. According to the preparation method, caspase-1 recombinant protein is used as an antigen for immunization of an animal so as to obtain the polyclonal antibody against chicken caspase-1. According to the invention, chicken caspase-1 is used as a research object; the polyclonal antibody against chicken caspase-1 prepared through gene amplification and construction, expression, purification and identification of a recombinant vector can specifically recognize target protein, so a foundation is laid for deep research on the toxicological significance of the family of chicken caspase-1 genes and the role played by the caspase-1 genes in virus-mediated innate immune response, and a novel target is provided for research on novel vaccines and development of medicines. Meanwhile, the prokaryotic expression vector constructed in the invention has high efficiency in expression of recombinant protein, and a separating and purifying method thereof is simple, low in cost and short in period.

The invention relates to a separation and purification method of umbilical cord mesenchymal stem cells and belongs to the technical field of stem cells and regenerative medicine. The method comprisesthe steps as follows: separation of umbilical cord tissue: taking the umbilical cord tissue, cleaning the umbilical cord tissue and removing blood, and shearing the tissue into tissue fragments for later use; tissue digestion: taking the tissue fragments, and adding tissue digestive juice for oscillation digestion, wherein the tissue digestive juice contains neutral protease, hyaluronidase, collagenase II and DNA enzyme; after digestion, adding a stop buffer for stop, performing filtration to obtain undigested tissue blocks and filtrate, and centrifuging the filtrate to remove supernatant to obtain cells; and cell culture: inoculating a culture flask with the tissue blocks and cells, using serum-free culture medium for culture to obtain the umbilical cord mesenchymal stem cells. The separation and purification method of umbilical cord mesenchymal stem cells overcomes the defects of an enzyme digestion method and a tissue block culture method in the prior art and can obtain enough primary cells within 3-5 days.

The invention discloses a synthetic method for p-hydroxybenzaldehyde. The synthetic method comprises the following steps: with phenol and formic acid as raw materials, subjecting the raw materials toan esterification reaction under the action of a catalyst A so as to produce phenyl formate; and subjecting phenyl formate to a Fries rearrangement reaction under the action of a catalyst B so as to produce p-hydroxybenzaldehyde. According to the invention, the conversion rate of the raw materials is high, product yield is high, and the method is novel method for preparing p-hydroxybenzaldehyde from phenol and formic acid; and phenol and formic acid undergo the esterification reaction under the action of the catalyst A so as to produce phenyl formate, phenyl formate undergoes the Fries rearrangement reaction under the action of the catalyst B so as to produce p-hydroxybenzaldehyde, and then recrystallization and purification are carried out, so product purity can reach 99% or more, and overall product yield is as high as 95%. The method uses widely available raw materials and cheap and easily available catalysts, and is simple in reaction process, free of severe requirements on production equipment, low in equipment investment and convenient for industrial application.

The invention discloses a sulfadimidine affinity membrane; a preparation method of the sulfadimidine affinity membrane comprises the following steps: (1) taking a cellulose acetate membrane, completely hydrolyzing under an alkaling condition, then adding epoxy chloropropane to perform epoxy activation, and then using deionized water to wash to be neutral after reaction under a suction filter condition, so as to obtain the epoxy activated membrane; (2) dissolving the sulfadimidine into an alkaline buffer solution with pH of 10-12, then dispersing the epoxy activated membrane obtained in the step (1) in the above-mentioned solution, reacting at the temperature of 50-65 DEG C for 24h-36h, then using deionized water to wash under the suction filter condition to make UV absorption of a percolate at 280nm close to zero, and then drying the solution to get the sulfadimidine affinity membrane. The sulfadimidine affinity membrane has good adsorption performance for an antibody, and is particularly suitable for separating and purifying immune globulin IgG.

The invention discloses carboxyl group-containing benzothiadiazide derivatives, a preparation method thereof, a medicine composition containing the compound and an application thereof in treatment or prevention for complications of vascular hypertension and serum hyperuricemia and / or hypercholesteremia. The benzothiadiazide derivative compounds meet a general formula (I) specified in the description. The carboxyl group-containing benzothiadiazide derivatives are synthesised by a one-step method, as well as are simple in synthesis process, simple in separation and purification methods, high in yield, good in repeatability, free of strong acid-resistant special equipment, and suitable for large-batch production.

The invention relates to the field of separation purification of natural products, and provides a method for efficiently separating and purifying caffeoylquinic acid isomers from mulberry leaves. Themethod comprises the following steps: performing ultrasonic wave enhanced extraction on the mulberry leaves, and performing separation purification by adopting an original pH induction liquid-liquid extraction technology, a macroporous adsorption resin enrichment technology and a high-speed countercurrent chromatography rapid separation technology to obtain the three caffeoylquinic acid isomers with purity of 93% or more. According to the method provided by the invention, caffeoylquinic acid rapidly enters an organic phase by the pH induction liquid-liquid extraction to reduce extraction times, so that the time is saved, chemical reagent consumption is reduced, and the industrial costs are reduced; and the method realizes the purpose of separating the caffeoylquinic acid isomers from the mulberry leaves by using the high-speed countercurrent chromatography rapid separation technology for the first time, the method has the advantages of simple steps, low solvent consumption, a short separation period, high product purity and a high yield, and is suitable for industrial production and application.

A preparation method for N-methylisatoic anhydride rapidly-labeled polysaccharide belongs to the technical field of fluorescent labeling. The preparation method employs a fluorescence tracer N-methylisatoic anhydride for labeling polysaccharide, and the method comprises: performing incubating on a N-methylisatoic anhydride stock solution and a polysaccharide solution under the condition of 35 DEG C for 15-20 min, and performing separation purification on the fluorescence-labeled polysaccharide through an organic solvent precipitation process. The reaction is mild in reaction conditions and short in reaction time, and the fluorescence-labeled polysaccharide separation purification process is simple. Polysaccharide labeled by fluorescence can be detected by using a routine fluorescence detector, and research on polysaccharide functions and mechanism is facilitated.

The invention provides a purifying method for a high-purity echinocandin B mother nucleus or a salt thereof. The purifying method comprises the following steps: dissolving a crude product of an echinocandin B mother nucleus or a salt thereof to prepare a sample and loading the sample into a preparative high-performance liquid chromatographic column; carrying out elution with mixed liquor of an aqueous solution and an organic solvent as a mobile phase; and fractionally collecting eluates. The purifying method is simple to operate, high in purifying efficiency and easy in process flow; the recovery rate of the high-purity echinocandin B mother nucleus or the salt thereof is more than 85%; a small amount of organic solvents are used, so the method is friendly to environment; the method has good process reproducibility, can realize mass product, is high and stable in recovery rate and saves cost; product purity reaches 99% or above; the process of separation and purification is simple; andthe concentration of an organic phase is low, so elution time is short, and cost is saved.

The invention provides a separation and purification method for a pertussis antigen. The separation and purification method comprises the steps of with diatomite and hydroxylapatite as column chromatography materials, after concentrating, regulating and conducting a bordetella pertussis fermentation solution, enriching the pertussis antigen by using the diatomite, and then, purifying by using the hydroxylapatite to obtain pertussis toxin (PT) and filamentous hemagglutinin (FHA). Proven through SDS-PAGE electrophoresis detection, the purities of both a PT protein and an FHA protein are higher than 95%. The method provided by the invention is simple in operation and capable of obtaining a high-purity antigen, not only greatly reducing the production cost, but also remarkably improving the quality of a product and increasing the yield of the product so as to be the simple and rapid separation and purification method for the pertussis antigen; in addition, the economic benefit is high, and the market application prospect is wide.