89results about How to "The separation and purification method is simple" patented technology

The invention relates to a synthesis method of nucleoside analogs, particularly to a synthesis method of an anti-virus-activity compound Entecavir. The invention also relates to intermediates for preparing Entecavir and a method for preparing the intermediates.

The invention relates to a synthesis method of nucleoside analogs, particularly to a synthesis method of an anti-virus-activity compound Entecavir. The invention also relates to intermediates for preparing Entecavir and a method for preparing the intermediates.

The invention relates to a synthesis method of nucleoside analogues, in particular to a synthesis method of a compound Entecavir with antiviral activity. The invention also relates to intermediates used for preparing Entecavir and methods for preparing the intermediates.

The invention relates to a synthesis method of a nucleoside analogue, in particular to a synthesis method of compound entecavir with antiviral activity. The invention also relates to midbodies for preparing the entecavir and a method for preparing the midbodies.

The invention discloses a synthesizing method of entikawei with antiviral activity, which relates to the intermediate to prepare entikawei and making method of the intermediate.

The present invention relates to anti-cancer active component of Huaiqing chrysanthemum and its separation and purification method. Its anti-cancer compound includes 9 beta-acetyl-costunolide (CP-C) whose molecular formula is C17H22O4 and 9 beta-acetyl-parthenclide (CP-I) whose molecular formula is C17H22O5. Said invention also provides the concrete steps for separation and purification of said anti-cancer components, and discloses three anti-cancer active components of CP-B, CP-C and CP-D.

The invention relates to a chemical synthetic method of a lipopeptide molecule, and especially relates to a lipopeptide molecule surfactant containing an azobenzene photosensitive group, and a synthetic method thereof. The lipopeptide with small molecular weight is synthesized through a liquid phase synthesis process, contains a small number of amino acids and can be easily synthesized through few reaction steps, and the synthesis efficiency and the purity of the lipopeptide with small molecular weight are high; the yield of every step in a synthesis route is high, and a separation and purification process is simple, so the consumption of a raw material and the waste of a product are reduced; and the azobenzene photosensitive group is effectively embedded into a fatty acid chain through the liquid phase synthesis process in order to bind a specific function group.

The invention belongs to the technical field of tobaccos, and particularly relates to a method for separating and purifying neophytadiene in flue-cured tobacco leaves. The method comprises the following steps: carrying out supercritical CO2 extraction on flue-cured tobacco leaves to obtain neophytadiene primary extract, mixing the neophytadiene primary extract with an alkaline solution to obtain an alkaline extracting solution, carrying out back extraction on the alkaline extracting solution to obtain an oil phase, and removing a back extraction agent in the oil phase to obtain high-purity neophytadiene, wherein the back extraction agent for back extraction is a mixture of petroleum ether and alkane or petroleum ether. The method provided by the invention is simple and easy to operate, andthe result of the embodiment shows that the yield of the neophytadiene obtained by the method provided by the invention reaches 15.74% and the purity reaches 97.99%.

The invention relates to a high-purity arsenic preparation method. The high-purity arsenic preparation method adopts an arsenic crude product as a raw material and orderly comprises 1, an oxidation reaction, 2, an esterification reaction, 3, solid-liquid separation, 4, adsorption purification, 5, solid-liquid separation, 6, rectification purification, 7, hydrolysis-crystallization, 8, solid-liquid separation, 9, water washing, 10, dehydration, 11, drying, and 12, a reduction reaction. The high-purity arsenic preparation method has the advantages of short process, simple separation purification processes, high product purity, stable quality, safe and reliable processes, low energy consumption and less three wastes and is a clean production method satisfying green chemical development requirements.

The invention discloses a novel method for separating and preparing salidroside from glossy privet fruit, which comprises the following steps: heating the medicinal material glossy privet fruit with ethanol under reflux to obtain an extracting solution; recycling the extracting solution under reduced pressure until no alcohol smell is emitted; centrifugalizing, carrying out alkaline hydrolysis on the supernatant, adding acid to regulate the solution to neutral state, carrying out primary separation with macroporous adsorbent resin, and separating by silica gel column chromatography to obtain a crude salidroside product; and purifying the crude product by a solvent method or column chromatography to obtain the white acicular crystals of salidroside. The determination on the salidroside by high efficiency liquid chromatography indicates that the purity is higher than 98%.

The invention relates to vibrio alginolyticus A-001 and a method for preparing agaro-oligosaccharide with agarase generated by the vibrio alginolyticus A-001. The vibrio alginolyticus A-001 is preserved in CCTCC (China Center for Type Culture Collection) in September 18, 2015, and a preservation number of the vibrio alginolyticus A-001 is CCTCC M2015555. The method includes: taking strains for culture, using a fermentation medium composed of a carbon source, a nitrogen source and inorganic salt to generate enzymes, salting out fermentation liquor through ammonium sulfate, centrifuging, dialyzing, passing through iron exchange columns and gel columns to obtain dry enzyme powder, allowing reaction between agarase and 0.5% agar substrate, filtering through an ultrafiltration membrane, collecting filtrate and performing freeze drying to obtain the agaro-oligosaccharide.

The invention relates to a synthesis method of 4-aminoquinaldine. The invention discloses the synthesis method of the 4-aminoquinaldine; the method comprises the following steps: enabling 2-methyl quinoline, taken as a starting raw material, to be subjected to a hydrogen peroxide oxidation reaction in acetic acid so as to generate 2-methylquinoline N-oxide; then, carrying out a nitration reactionto generate 2-methyl-4-nitroquinoline N-oxide; after that, illuminating for carrying out a reaction to generate 2-methyl-4-nitroquinoline; producing 2-methyl-4-aminoquinoline N-oxide by means of interaction of ferric trichloride hexahydrate and hydrazine hydrate in ethanol; finally, carrying out a reduction reaction by using iron powder under the action of acetic acid so as to generate the 4-aminoquinaldine. The synthesis method provided by the invention has the beneficial effects that the used raw materials and reagents are low in price and easy to obtain, the synthesis method is simple and feasible, the reaction conditions are mild, and a simple and convenient synthetic route is opened up to the synthesis of the 4-aminoquinaldine.

The invention discloses a preparation method of a porcine TLR4 polyclonal antibody. According to the preparation method, recombinant TLR4 protein (antigen) and freund's adjuvant are emulsified, then an immune animal obtains polyclonal antibody serum, wherein the recombinant TLR4 protein has the amino acid sequence as indicated in the sequence table SEQ. ID. No. 1. It is shown through tests that the antibody prepared through the method is good in reactivity and specificity; due to the fact that a TLR4 gene is a member of a significant pattern recognition receptor Toll-like receptor family targeted to innate immune response, the porcine TLR4 polyclonal antibody can be applied to detection of the porcine TLR4 protein and related research; a good foundation is laid for researching the interaction mechanism between porcine bacterial and viral infection diseases and TLR4 and the virus immunity escape mechanism, and a new target is provided for developing new generation vaccine and medicine. Meanwhile, a prokaryotic expression vector constructed through the method is high in recombinant protein expression efficiency, and the separation and purification method is simple and easy to operate.

The invention provides a method for processing a plant seed mixture, and belongs to the field of seed processing. After a seed mixture and sand are mixed, vibrated and rubbed, seeds are separated from pectin, and further filtering, separation and washing are performed to obtain seeds. On the basis of a physical mixing vibration method, chemical treatment and fermentation methods in the prior art are avoided, and the seed yield and the seed quality are improved.

The invention relates to a separation and purification method of umbilical cord mesenchymal stem cells and belongs to the technical field of stem cells and regenerative medicine. The method comprisesthe steps as follows: separation of umbilical cord tissue: taking the umbilical cord tissue, cleaning the umbilical cord tissue and removing blood, and shearing the tissue into tissue fragments for later use; tissue digestion: taking the tissue fragments, and adding tissue digestive juice for oscillation digestion, wherein the tissue digestive juice contains neutral protease, hyaluronidase, collagenase II and DNA enzyme; after digestion, adding a stop buffer for stop, performing filtration to obtain undigested tissue blocks and filtrate, and centrifuging the filtrate to remove supernatant to obtain cells; and cell culture: inoculating a culture flask with the tissue blocks and cells, using serum-free culture medium for culture to obtain the umbilical cord mesenchymal stem cells. The separation and purification method of umbilical cord mesenchymal stem cells overcomes the defects of an enzyme digestion method and a tissue block culture method in the prior art and can obtain enough primary cells within 3-5 days.

The invention discloses a synthetic method for p-hydroxybenzaldehyde. The synthetic method comprises the following steps: with phenol and formic acid as raw materials, subjecting the raw materials toan esterification reaction under the action of a catalyst A so as to produce phenyl formate; and subjecting phenyl formate to a Fries rearrangement reaction under the action of a catalyst B so as to produce p-hydroxybenzaldehyde. According to the invention, the conversion rate of the raw materials is high, product yield is high, and the method is novel method for preparing p-hydroxybenzaldehyde from phenol and formic acid; and phenol and formic acid undergo the esterification reaction under the action of the catalyst A so as to produce phenyl formate, phenyl formate undergoes the Fries rearrangement reaction under the action of the catalyst B so as to produce p-hydroxybenzaldehyde, and then recrystallization and purification are carried out, so product purity can reach 99% or more, and overall product yield is as high as 95%. The method uses widely available raw materials and cheap and easily available catalysts, and is simple in reaction process, free of severe requirements on production equipment, low in equipment investment and convenient for industrial application.

The invention discloses carboxyl group-containing benzothiadiazide derivatives, a preparation method thereof, a medicine composition containing the compound and an application thereof in treatment or prevention for complications of vascular hypertension and serum hyperuricemia and/or hypercholesteremia. The benzothiadiazide derivative compounds meet a general formula (I) specified in the description. The carboxyl group-containing benzothiadiazide derivatives are synthesised by a one-step method, as well as are simple in synthesis process, simple in separation and purification methods, high in yield, good in repeatability, free of strong acid-resistant special equipment, and suitable for large-batch production.

The invention relates to the field of separation purification of natural products, and provides a method for efficiently separating and purifying caffeoylquinic acid isomers from mulberry leaves. Themethod comprises the following steps: performing ultrasonic wave enhanced extraction on the mulberry leaves, and performing separation purification by adopting an original pH induction liquid-liquid extraction technology, a macroporous adsorption resin enrichment technology and a high-speed countercurrent chromatography rapid separation technology to obtain the three caffeoylquinic acid isomers with purity of 93% or more. According to the method provided by the invention, caffeoylquinic acid rapidly enters an organic phase by the pH induction liquid-liquid extraction to reduce extraction times, so that the time is saved, chemical reagent consumption is reduced, and the industrial costs are reduced; and the method realizes the purpose of separating the caffeoylquinic acid isomers from the mulberry leaves by using the high-speed countercurrent chromatography rapid separation technology for the first time, the method has the advantages of simple steps, low solvent consumption, a short separation period, high product purity and a high yield, and is suitable for industrial production and application.

The invention provides a purifying method for a high-purity echinocandin B mother nucleus or a salt thereof. The purifying method comprises the following steps: dissolving a crude product of an echinocandin B mother nucleus or a salt thereof to prepare a sample and loading the sample into a preparative high-performance liquid chromatographic column; carrying out elution with mixed liquor of an aqueous solution and an organic solvent as a mobile phase; and fractionally collecting eluates. The purifying method is simple to operate, high in purifying efficiency and easy in process flow; the recovery rate of the high-purity echinocandin B mother nucleus or the salt thereof is more than 85%; a small amount of organic solvents are used, so the method is friendly to environment; the method has good process reproducibility, can realize mass product, is high and stable in recovery rate and saves cost; product purity reaches 99% or above; the process of separation and purification is simple; andthe concentration of an organic phase is low, so elution time is short, and cost is saved.