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Anti-cance active substance of cyathocline purpurea and separating and purifying method thereof

A technology of anti-cancer activity and cup chrysanthemum, which is applied in the field of extraction of anti-cancer active ingredients and their separation and purification

Inactive Publication Date: 2004-06-30
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, there is no other report on the chemical constituents of the genus Chrysanthemum and its application

Method used

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  • Anti-cance active substance of cyathocline purpurea and separating and purifying method thereof
  • Anti-cance active substance of cyathocline purpurea and separating and purifying method thereof
  • Anti-cance active substance of cyathocline purpurea and separating and purifying method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0207] 1. Dry and pulverize the whole plant of Chrysanthemum chrysanthemum, take 10 kg of pulverized sample, soak it in 95% ethanol for 30 days, extract the extract, and obtain the extract CP 0 ≈800 grams;

[0208] 2. Take 800 grams of this extract, mix it with diatomaceous earth in a ratio of 1:1, stir and mix evenly with 95% ethanol, dry it on a water bath, weigh it, W 样 =710 grams, with a 1L Soxhlet extractor, extract successively with sherwood oil, chloroform: ethyl acetate=3:1, ethyl acetate, acetone, methanol respectively, get crude component CP 1 (120g), CP 2 (100g), CP 3 (100g), CP 4 (130g), CP 5 (150 grams), such as figure 1 shown.

[0209] 3. Use L 1210 CP 1 ~CP 5 Cytotoxicity activity screening was carried out. As a result, the anticancer activity was in the order of CP 2 >CP 3 >CP 1 >CP 4 >CP 5 .

[0210] 4. To CP 2 Carry out column chromatography, use 40-63μm silica gel as the adsorbent, use petroleum ether-ethyl acetate-methanol as the eluent, an...

Embodiment 2

[0214] 1, with embodiment 1;

[0215] 2. Take 800 grams of this extract, mix it with diatomaceous earth in a ratio of 1:2, stir and mix evenly with 95% ethanol, dry it on a water bath, weigh it, W 样 =710 grams, with 1L Soxhlet extractor, extract successively with sherwood oil, chloroform: ethyl acetate=1:0, ethyl acetate, acetone, methyl alcohol respectively, obtain crude component CP 1 (120g), CP 2 (80g), CP 3 (120g), CP 4 (130g), CP 5 (150 grams), such as figure 1 shown.

[0216] 3,4,5,6 are identical with embodiment 1;

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Abstract

The present invention relates to anti-cancer active component of Huaiqing chrysanthemum and its separation and purification method. Its anti-cancer compound includes 9 beta-acetyl-costunolide (CP-C) whose molecular formula is C17H22O4 and 9 beta-acetyl-parthenclide (CP-I) whose molecular formula is C17H22O5. Said invention also provides the concrete steps for separation and purification of said anti-cancer components, and discloses three anti-cancer active components of CP-B, CP-C and CP-D.

Description

technical field [0001] The invention relates to the technical field of medical medicines, in particular to extracting anticancer active ingredients from Chrysanthemum chinensis and a separation and purification method thereof. Background technique [0002] Cup chrysanthemum is a plant of the genus Cyathocline Purpurea Buch.-Ham.ex De Don in the family Asteraceae. There are about 3 species of plants of the genus Cyathocline Purpurea in the world, and 1 species in China [Lin Rong, Chen Yilin. Flora of China (Seventh Fourteen volumes). Beijing: Science Press, 81-82]. Cup chrysanthemum is mainly distributed in the subtropical regions of Southwest China and India. [0003] The whole plant of Chrysanthemum chrysanthemum is used as medicine, and it is used by folks to reduce inflammation and stop bleeding, prevent and treat various inflammations, colds, and high fever. [0004] The domestic and foreign research overview of the chemical constituents of the genus Chrysanthemum: ...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P35/00C07D307/94C07D493/04
Inventor 李祖强
Owner YUNNAN UNIV
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