Sulfadimidine affinity membrane and application of sulfadimidine affinity membrane in antibody separation and purification
A sulfamethazine, separation and purification technology, applied in the field of biomedical materials, can solve the problems of low purity, small processing capacity, long time, etc., and achieve high purity and activity, mild operating conditions, and high selectivity. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Example 1: Preparation of Sulfamethazine Affinity Membrane
[0030] (1) Disperse 10 pieces of cellulose acetate membranes (Hangzhou Kenuo, Φ=10cm) in 100mL NaOH solution with a concentration of 2mol / L, and hydrolyze it in a water bath vibrating shaker (175rpm) at 70°C for 1.5h. After cooling down to 40°C, add a solution of epichlorohydrin with a volume fraction of 20%, and react at 40°C for 4 hours. After the reaction, wash with deionized water to neutral under suction filtration conditions to obtain an epoxy-activated membrane.
[0031] (2) Dissolve 1.0 g of sulfamethazine in a PBS buffer solution of pH 10.5, 20 mmol / L, and disperse 10 pieces (about 5 g) of the epoxy-activated film obtained in step (1) in the buffer solution , shake (175rpm) in a reaction water bath at 55°C for 36h. Then wash with deionized water under the condition of suction filtration until the ultraviolet absorption of the filtrate at 280nm is close to 0, and after drying, the sulfamethazine affin...
Embodiment 2
[0034] Example 2: Application of Sulfamethazine Affinity Membrane in Antibody Separation and Purification
[0035] Get the sulfamethazine affinity membrane 10~15 that embodiment 1 makes, it is packed in the film cup (such as figure 1 ), dilute 10ml of ascites with pH 5.5, 20mmol / L PBS buffer solution to 100mL, and then use a peristaltic pump to pump it into the membrane cup at a flow rate of 2ml / min to allow it to fully adsorb with the ligand on the membrane. Deionized water was pumped in at a flow rate until the filtrate had near zero UV absorbance at 280 nm. Then the elution buffer (PBS of 20 mmol / L at pH 5.5+1.5 mol / L NaCl) was pumped in at the same flow rate. The eluted substances were collected, centrifuged at 3000rpm for 15min with an ultrafiltration centrifuge tube, then distributed into 1.5mL tubes, and frozen for preservation.
[0036] The purity of the antibody was detected by SDS-PAGE, and the results were as follows image 3 As shown, 1 indicates protein marker,...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com