111 results about "Sulfadimidine" patented technology

The invention discloses a temperature-sensitive magnetic sulfadimidine molecular imprinted adsorbent as well as a preparation method and application thereof, belonging to the technical field of preparation of environment functional materials. A gamma-Fe2O3/SiO2 magnetic composite material is prepared by using a sol-gel method and is subjected to vinyl modification by using 3-(methylacryloyloxy) trimethoxypropylsilane; with a vinyl-modified magnetic composite material (MPS (Modified Polystyrene)-gamma-Fe2O3/SiO2) as a substrate material, sulfadimidine as a template molecule, acrylamide as a functional monomer, N-isopropylacrylamide as a temperature-sensitive monomer, ethylene glycol-bis(methylacrylic acid) ester as a cross-linking agent and 2,2'-azobisisobutyronitrile as an initiator, a temperature-sensitive magnetic molecular imprinted polymer is prepared by using a free group polymerization process and is applied to selective recognition and separation of the sulfadimidine in an aqueous solution; and the obtained polymer has the advantages of remarkable heat and magnet stability, sensitive magnet and heat inducting effect, higher adsorption capacity, reversible adsorption/release function along with the temperature and remarkable recognition performance of a sulfadimidine molecule.

A detection method simultaneously detects residue of multiple synthetic antibacterial agents in aquatic products by a high performance liquid chromatography. The synthetic antibacterial agents mainly comprise sulfadiazine SD, sulfamerazine SMR, sulfadimidine SDD, sulfadimethoxine SDM, furazolidone FZD, oxolinic acid OXA, nalidixic acid NAA and the like. The multiple synthetic antibacterial agents in an aquatic product sample are extracted by acetonitrile, degreased by normal hexane, concentrated, purified by a solid phase extraction column (alumina neutral) or a C18 solid phase extraction column, analyzed by a liquid chromatograph, detected by an ultraviolet detector, and quantified by an external standard method. The method can quickly, sensitively and accurately detect multiple target compounds to greatly reduce detection cost and shorten detection time.

The invention provides a preparation method and application of a graphene/carbon nano-tube composite. The preparation method comprises the following steps: oxidizing graphene powder and a multiwalled carbon nano-tube together to obtain graphene oxide and an oxidized carbon nano-tube; carrying out ultrasonic peeling and dispersion on the graphene oxide and the oxidized carbon nano-tube; carrying out modification connection and reduction on the graphene oxide and the oxidized carbon nano-tube with ammonia water and ethylenediamine; and filtering, washing and drying a product after reaction to obtain the graphene/carbon nano-tube composite. The preparation method is simple and convenient, and reaction conditions are mild; in the preparation method, the graphene is connected with the carbon nano-tube through covalent modification, and the carbon nano-tube is connected between graphene slice layers. The graphene/carbon nano-tube composite prepared by the method has a fluffy and porous three-dimensional skeleton structure, and can be used for extracting and detecting melamine, clenbuterol, sulfadimidine sodium, indoleacetic acid, bambuterol, clorprenaline, dicofol, 2,2-bis(4-chlorophenyl)-1,1-dichloroethane or chlorofluoro thiochromanone.

The invention relates to a synthetic method of deuterium-marked sulfanilamide. The method comprises the following steps of: reacting deuterated sulfonic acid with N-chlorosuccinimide (NCS) to obtain deuterated sulfonyl chloride which is not required to be separated; and adding amine for undergoing an amination reaction under the action of a metal catalyst to obtain deuterium-marked sulfanilamide. Compared with the prior art, the method has the advantages that: acyl chloride synthesis and the amination reaction are performed with a 'one pot process', operation is simple and convenient, the yield is high, and a synthesized product comprises sulfapyridine-benzene ring-D4, sulfadimidine-benzene ring-D4, sulfameter-benzene ring-D4, sulfaquinoxaline-benzene ring-D4, sulfisoxazole-benzene ring-D4 and sulfamethoxazole-benzene ring-D4.

The invention discloses application of papermaking sludge based biological carbon in removal of antibiotics or heavy metals and the antibiotics from a water body and belongs to the technical field ofbiomass treatment and water treatment. The papermaking sludge based biological carbon has larger specific area and a larger pore size and can efficiently remove combined pollution of copper, zinc, arsenic, sulfadimidine, tylosin and roxithromycin. The biological carbon is simple in preparation technology, significant and stable in effect, environment-friendly and free from secondary pollution, andhas a wide application value in a heavy metal/antibiotic pollution repair aspect in the water body. Paper mill sludge difficult to treat is recycled; the environmental pollution problems of wastewater containing the heavy metals and the antibiotics are solved; and therefore, the application has important environmental and economic benefits. The biological carbon has a huge market and an application potential in waste recycling and sewage treatment.

The present invention is one kind of affinity chromatographic stuffing with sulfadimidine as ligand specially for separating antigen globin. Sulfadimidine is one kind of widely applied sterilizing medicine, and its amino group may be utilized to bond to various carrier to prepare affinity chromatographic stuffing. The stuffing is used in specifically separating gamma antigen globin from plasma, fermented liquid, tissue homogenizing liquid and other matter containing gamma antigen globin, with purity reaching 91 %. The immobilized sulfadimidine and human IgG have interaction dissociation constant up to 3E(-6) mol/L. The new affinity chromatographic stuffing has high affinity, high safety, low cost and other features.

The invention discloses a preparation method and application of sulphonamide molecular-imprinting solid-phase extraction columella. The preparation method comprises the following steps of: (1) mixing a template molecule, a functional monomer and a cross-linking agent; (2) representing molecular-imprinting polymer particles; (3) filling the molecular-imprinting polymer particles into a solid-phase extraction columella, wetting and washing by using methanol and acetone, sealing in vacuum, wherein the sulphonamide molecular-imprinting polymer is obtained by the following steps of: (a) dissolving the template molecule sulfanilamide and the functional monomer in pore-foaming agent acetonitrile to obtain a mixed solution; (b) carrying out ultrasonic treatment onto the mixed solution by an ultrasonic cleaner to obtain a pre-polymerization system; (c) transferring the pre-polymerized system to a quartz reaction test tube, adding cross-linking agent and imitator; (d) placing the pre-polymerization system under ultraviolet light; (e) directly grinding the imprinted polymer to obtain the imprinted polymer particles. The preparation method of the sulphonamide molecular-imprinting solid-phase extraction columella is high in recovery rate, simple to prepare, low in cost and capable of being directly used for selective adsorption and efficient enrichment of sulfadiazine, sulfamerazine and sulfadimidine in animal-origin food.

The invention relates to a sulfadimidine degrading bacterium S-07 degraded by microorganisms and a screening method thereof. The sulfadimidine degrading bacterium S-07 is verified as Geobacillus, and the Genbank registration number of the bacterium 16S rDNA is KU588289. The sulfadimidine degrading bacterium is preserved in the China General Microbiological Culture Collection Center on April 21st, 2016, and the preservation number of the sulfadimidine degrading bacterium is CGMCC No. 12382. The sulfadimidine degrading bacterium S-07 well grows within the temperature range of 50-75 DEG C, has quite strong tolerance to sulfadimidine, and can efficiently degrade sulfadimidine in a liquid phase and a solid phase. The bacterium can be used for degrading and removing sulfadimidine residues in livestock excrement or sludge high-temperature compost, the removal rate is larger than 95%, and the sulfadimidine degrading bacterium S-07 has good industrial application prospects and environment benefits.

The invention discloses a set of sulfamethazine specifically-bound aptamers and a screening method and applications thereof. The aptamer for identifying sulfamethazine, disclosed by the invention, is a single-stranded DNA molecule shown in any one of formulas (1)-(4): (1) a single-stranded DNA molecule shown in a sequence 3 in a sequence table; (2) a single-stranded DNA molecule shown in a sequence 1 in the sequence table; (3) a single-stranded DNA molecule shown in a sequence 2 in the sequence table; and (4) a single-stranded DNA molecule shown in a sequence 4 in the sequence table. Experiments show that a directly competitive chemiluminescence analysis method established based on biotinylated aptamers and used for detecting sulfamethazine provides a basis for the practical application of the aptamers of sulfamethazine, and provides technical support for more efficient and extensive monitoring of antimicrobial drug residues.

The invention discloses a molecular imprinting-Raman combination detection method of sulfadimidine in milk. The method can realize rapid low-cost detection of excess sulfadimidine in milk. A hydrogel prepared through a molecular imprinting technology is used to complete adsorption and enrichment of sulfadimidine in a detected sample in 5min, and a portable Raman spectrometer is used to collect spectrum information, so the whole process is rapid and convenient; and the hydrogel can be repeatedly used, so the detection cost is reduced, environmental protection is realized, and onsite high-flux detection requirements are met.

The invention relates to a magnetic molecular imprinting electrochemical sensor for detecting trace sulfadimidine. The magnetic molecular imprinting electrochemical sensor of sulfadimidine is prepared by adopting a magnetic reduced graphene modified magnetic electrode and applying dopamine to carry out self-polymerization. When the concentration of olaquindox is in a range of 10.0nM-200.0nM, an electrochemical signal rising degree has a good linear relation with the concentration of sulfadimidine; and the detection limit of a method reaches 0.1nM. When the sensor is used for detecting an actual sample, the yield reaches 92.6%-101.7%; and with the adoption of the magnetic molecular imprinting electrochemical sensor, the sensitivity and selectivity of sulfadimidine are improved, and the automation of detection of trace sulfadimidine is easy to realize.

The invention discloses a forensic medicine diatom test method.The forensic medicine diatom test method comprises the following steps that sulfamethazine, acrylic acid, acrylamide, N'N-methylene bisacrylamide, ammonium persulfate and deionized water are taken according to a proportion and fully mixed in a beaker; after oxygen is removed with nitrogen, the mixture is poured into a sandwich space formed by two glass slides and then put into an oven at the temperature of 60 DEG C for a polymerization reaction for 3 h, and film-state imprinting hydrogel is prepared; the obtained imprinting hydrogel is soaked in a 10% acetic acid solution, template molecules sulfadimidine in the imprinting hydrogel are removed through ultrasonic oscillation, then the imprinting hydrogel is soaked in a sample to be tested for 5-10 min and then taken out, and then the imprinting hydrogel is put under an optical microscope for observation; diatom on the filter film or a shot picture is checked, classified and counted in a manual recognition mode or an automatic computer recognition mode.The forensic medicine diatom test method is high in yield, efficiency and performance cost ratio, easy to popularize, accurate in qualitative and quantitative analysis and low in labor intensity.

The invention discloses a compound sodium sulfadimidine injection liquid for a pig and a preparation method thereof, aiming at providing a compound kanamycin sulfate injection liquid which has fast effect for treating toxoplasmosis of the pig, addresses both the symptoms and root causes, reduces the time and dosage of used drug and has convenient drug usage, and a preparation method with simple technique and easy implementation. Each 100L injection liquid comprises: 5 to 20 sodium sulfadimidine, 1 to 10kg of lincomycin hydrochloride, 5 to 20kg of doxofylline, 1 to 4kg of trimethoprim, 0.2kg of sodium bisulfite, 0.01kg of EDTA-2Na, 40kg of propylene glycol, 10kg of 95% alcohol and the balance of water for injection. The injection liquid has fast effect for toxoplasmosis of the pig, addresses both the symptoms and root causes, can effectively treat and prevent secondary infection of bacterium, reduces drug resistance of pathogenic microorganism and leads the pig to accelerate restoring. Simultaneously, the compound sodium sulfadimidine injection liquid is an injection liquid and has convenient use.

The invention relates to a soluble powder for treating poultry respiratory tract infection, comprising tilmicosin, sulfadimidine sodium and auxiliary materials, the medicament of the invention is taken as antibiotic series medicament and mainly used for treating various respiratory infection caused by sensitive bacteria and poultry chronic respiratory disease, the invention combines the sulfadimidine sodium with the tilmicosin for using and acting on different sensitive bacteria simultaneously, solves the problem of generating medicament resistance after singly using the tylosin to treat the disease, the invention has good oral administration absorption effect and high safety.

The invention relates to a sulfamethazine enzyme-linked immunoassay kit. A kit body comprises an elisa plate coated with sulfamethazine, a sulfamethazine specific antibody, enzyme conjugate, a sulfamethazine standard substance solution, a substrate A color-developing solution and a substrate B color-developing solution, a stop solution, concentrated washing liquid, concentrated complex liquid, and a concentrated extracting solution. The kit solves the problems of long operation time and complex pretreatment step in other kits, only 30 minutes is required by a whole operation process, and a special instrument is not required to be arranged on equipment during pretreatment; the kit can be operated easily and quickly and is suitable for field large-scale screening; and all reagents in the kit are working liquid, so the kit is easy to operate and is practicable.

The invention relates to a powder agent for treating livestock and poultry mycoplasma infection, comprising tylosin tartrate, sulfadimidine sodium and auxiliary materials. The medicament of the invention can be mainly used for preventing and treating the mycoplasma disease of the livestock and poultry, meanwhile the sulfadimidine sodium is added in the preparation for controlling the secondary infection mixed infection caused by the mycoplasma, the medicament has good oral administration absorption effect and high safety, can be suitable for popularization and application in the animal production.

The invention relates to a process for producing sulfadimidine. Sulfaguanidine and acetylacetone are taken as raw materials; and the process comprises the following steps of: 1, performing condensation reaction to obtain a wet product of sulfadimidine; 2, washing with hot water to obtain a wet product of sulfadimidine, and removing other inclusions in a reaction solvent; 3, adding active carbon and caustic soda liquid into the washed sulfadimidine and performing alkali dissolution decoloration; 4, neutralizing caustic soda liquid brought by the decolored sulfadimidine by using an acid solvent, and crystallizing; and 5, separating out crystallized sulfadimidine by adopting a centrifugal analysis method, and drying to obtain a finished product. The process is characterized in that: in the neutralizing and crystallizing step, the acid solvent neutralization is hydrochloric acid neutralization. In the whole chemical production, hydrochloric acid is taken as a byproduct and can be recycled massively; and the hydrochloric acid replaces the conventional acetic acid neutralization solvent, which is more economic, and the quality of the obtained product is improved.

The invention provides a sulfadimidine nucleic acid aptamer screening method and a sulfadimidine nucleic acid aptamer detection kit. The kit comprises a fluorescence aptamer sensor based on graphene oxide, wherein the fluorescence aptamer sensor based on graphene oxide comprises an aptamer marked by an FAM fluorophore and graphene oxide, wherein the sequence of the sulfadimidine aptamer marked bythe FAM fluorophore is No 1s recorded in a nucleotide sequence table. The invention discloses an application of the sulfadimidine fluorescence detection kit in quantitative detection of sulfadimidinein test samples of milk or eggs.

The invention discloses a degradation method for sulfadimidine in water. Biochar made from a biomass raw material is used as a raw material, by loading TiO2, a composite biochar material is prepared,and by utilizing a thermal processing technology, the composite biochar material is converted into a biochar adsorption material; then, with assistance of electron beam irradiation, the synthesized biochar adsorption material is utilized for efficiently degrading the sulfadimidine in a solution, and through irradiation, active free radicals are generated, and the high reduction performance is achieved; the free radicals react with groups and carbon rings of the sulfadimidine, and finally, non-toxic carbon dioxide, nitrogen and water molecules are generated. According to the method, by adding the biochar adsorption material, efficient antibiotic degradation is achieved under the low radiation condition, the electron beam irradiation has a good degradation effect on the sulfadimidine, the demand of the first order kinetics is met, and R is greater than 0.99. The prepared biochar adsorption material has good recycling performance, and the method has the advantages of short consumed degradation time, high recycling performance, low cost and the like.

The invention discloses a chemiluminescence detection kit for sulfadimidine and a preparation method of the chemiluminescence detection kit. The kit comprises magnetic particles coupled with sulfadimidine antigens or antibodies, an acridine ester marker, sulfadimidine series standard substances, a chemiluminescence pre-excitation solution A, a chemiluminescence excitation solution B and a cleaning solution. Compared with an existing technology for detecting sulfadimidine, the kit has the advantages of being high in sensitivity, high in signal-to-noise ratio and quick in detection. The analysis sensitivity can reach 0. 020 microgram per liter.

The invention relates to a method of extracting medicine residues of seven sulfonamides from chicken by utilization of an ionic liquid and achieving an objective of purification during extraction purification, through adjusting the pH value, the water amount and the salt amount in the ionic liquid to achieve homogeneous phase extraction and layered purification, wherein the seven sulfonamides residues comprise sulfathiazole (ST), sulfamerazine (SMR), sulfamethizole (STZ), sulfadimidine (SDMD), sulfachlorpyridazine (SCP), sulfamethoxazolum (SMZ), and sulfisoxazole (SIX). Liquid chromatographic analysis results show that the recovery rate of each of the sulfonamides is 82.1%-108%, and the relative standard deviation is 0.5%-5.9%. The green and simple extracting method is established so as to reduce pollution or harm of traditional organic solvent extraction methods to the environment and to experiment persons.

The invention discloses a detection kit for detecting sulfadimidine in food. The detection kit is prepared from enzyme-labeled antigen, enzyme-labeled antigen diluent, a magnetic-labeled antibody, magnetic-labeled antibody diluent, a sulfadimidine series standard substance solution, chemiluminescent substrate A liquid, chemiluminescent substrate B liquid, a concentrated complex solution and concentrated washing liquid. The detection kit provided by the invention has higher sensitivity and specificity; the detection sensitivity to the sulfadimidine can reach 0.025mu g/L.

The invention discloses a method for simultaneous determination of nine sulfonamide antibiotics on suspended particles in water. The method comprises the following steps: taking a target water sample,filtering, and collecting a filtered membrane and particles retained on the surface; adding a quantitative internal standard substance; cutting and placing in a centrifuge tube after freeze drying; adding an appropriate amount of extractant; performing constant temperature oscillation, ultrasonic treatment and centrifuging; transferring supernatant to a K-D concentration bottle; purging the extraction liquid to less than 1ml by nitrogen gas; filtering with an organic microporous filter membrane; and measuring with an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer. The method has the advantages of short sample pretreatment time, simple steps, simultaneous determination of the contents of nine sulfonamide antibiotics such as sulfadiazine, sulfapyridine, sulfamethazine, sulfisomidine, sulfachlorpyridazine, sulfamerazine, sulfamethoxazole, sulfaquinoxaline and trimethoprim adsorbed on the suspended particles in water, and more accurate and true reflection of the contents of the sulfonamide antibiotics in water more.

The invention discloses a preparation method of an immunoaffinity column. The immunoaffinity column is a compound affinity column which is mainly prepared from three types of immunoaffinity columns through mixed packing in a volume ratio being 1:1:1, and the three types of immunoaffinity columns are prepared from three antibodies and a coupling matrix through coupling and wet packing. The column capacity of the immunoaffinity column is 2,500 ng, the immunoaffinity column is good in purification effect and can be repeatedly used three times, and average recovery rate is 67%-107%. The compound immunoaffinity column has good specific adsorption property for at least 12 sulfonamides including sulfaquinoxaline, sulfadiazine, sulfapyridine, sulfametoxydiazine, sulfadimidine, sulfamethoxazole, sulfafurazole, sulfadimethoxypyrimidine, sulfathiazole, sulfamerazine, sodium N-sulphanilamidate and sulfamonomethoxine. The immunoaffinity column which is high in coupling rate, specific adsorption property, column capacity and recovery rate is prepared, and a sensitive, rapid and simple analysis method capable of detecting residues of the 12 sulfonamides simultaneously is provided.