Method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc
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A technology of seabuckthorn flavonoids and large fruit seabuckthorn, which is applied in the field of separation and purification of seabuckthorn flavonoids, can solve the problems of difficult industrial production, easily polluted membranes, complex processes, etc., and achieve low production cost, low cost, and high purification efficiency.
Inactive Publication Date: 2012-07-18
QIQIHAR UNIVERSITY
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Problems solved by technology
However, the process is complicated, and the membrane is very easy to be polluted, resulting in high cost, and it is difficult to realize industrial production
Method used
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Embodiment 1
[0022] (1) Raw material degreasing
[0023] Weigh 120g of seabuckthorn pomace powder with a particle size of 40 mesh and place it in a Soxhlet extractor, reflux with low-boiling point petroleum ether at 70°C for 2 hours, remove the fat in the seabuckthorn pomace, and recover the solvent.
[0024] (2) Separation and extraction of flavonoids from seabuckthorn
[0025] Take 100g of degreased seabuckthorn powder, add 1.5L of 85% ethanol aqueous solution, ultrasonicate for 30min under the conditions of ultrasonic power 300w, frequency 40KHZ, temperature 50°C, extract three times, add diatomaceous earth to filter, and combine the extracts to obtain a clear Sea buckthorn flavonoid extract 2.5L.
[0026] (3) Purification of flavonoids from seabuckthorn
[0027] Add 12.5g of zinc sulfate to the extract to carry out the complexation reaction, the stirring speed is 150r / min, adjust the pH value of the solution to 8.5 with 4% NaOH solution while stirring, centrifuge at 4000r / min for 10m...
Embodiment 2
[0029] (1) Raw material degreasing
[0030] Weigh 100g of seabuckthorn pomace powder with a particle size of 40 mesh and place it in a Soxhlet extractor, reflux with low-boiling point petroleum ether at 70°C for 2 hours, remove the fat in the seabuckthorn pomace, and recover the solvent.
[0031] (2) Separation and extraction of flavonoids from seabuckthorn
[0032] Take 80g of degreased seabuckthorn powder, add 1.2L of 85% ethanol aqueous solution, ultrasonicate for 30min under the conditions of ultrasonic power 300w, frequency 40KHZ, temperature 50°C, extract three times, add diatomaceous earth to filter, and combine the extracts to obtain a clear Sea buckthorn flavonoid extract 2.0L.
[0033] (3) Purification of flavonoids from seabuckthorn
[0034]Add 10.0 g of zinc acetate to the extract for complexation reaction, stirring at 150 r / min, adjust the pH of the solution to 7.5 with 4% NaOH solution while stirring, centrifuge at 4000 r / min, and collect the precipitate. The ...
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Abstract
A method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc. The invention relates to a method for separating purified seabuckthorn flvonoid from large berry seabuckthorn marc. The invention solves the problems in the existing separation and purification method of seabuckthorn flavonoid, such as tedious method, complicated techniques, high cost of separation and purification, easy damage to the activity of flavonoid and low purity of flavonoid. The method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc disclosed by the invention comprises the following steps: first, degreasing the raw materials; second, ultrasonic wave extracting and centrifugating; third, adding metal salt to extracting solution to lead complex reaction between flavonoid and metal, agitating, tempering the pH of solution and collecting sediment; and fourth, adding decomplexing additive to resolve sediment, concentrating in a decompressing way to dryness, using ethanol to dissolve and filter, concentrating filtrate, and obtaining the separated and purified seabuckthorn flavonoid powder after drying. The method for separating purified seabuckthorn flavonoid from large berry seabuckthorn marc disclosed by the invention can ensure the full improvement of the purity of the purified seabuckthorn flavonoid, and maintains the original biological activity of the natural flavonoid, the seabuckthorn flavonoi is safe and nontoxic, and the method disclosed by the invention has the advantages of low production cost and simple process, and is suitable for the large-scale production of the seabuckthorn flavonoid.
Description
[0001] Technical field: the present invention relates to a method for separating and purifying seabuckthorn flavonoids from seabuckthorn pomace. Background technique: [0002] Seabuckthorn (Hippophae rhamnoides L.) is a perennial deciduous shrub or small tree belonging to the genus Hippophae Linn. in the family Elaeagnaceae. Originally produced in my country and Europe, it is distributed in temperate, cold temperate and subtropical alpine regions of Eurasia. It was most distributed in the former Soviet Union, Mongolia and China. The species mainly include rib fruit seabuckthorn, Tibetan seabuckthorn, Mongolian seabuckthorn, willow-leaf seabuckthorn, etc. The large-fruited seabuckthorn is a new variety introduced from Russia in recent years and selected through hybridization. It is mainly planted in Heilongjiang. Its main feature is that the fruit is large. , Nutritious, high flavonoid content. [0003] As an ancient wild plant, seabuckthorn has been used by humans since ancie...
Claims
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