124 results about "Citrus x limonia" patented technology

The invention discloses a digestion-promoting and intestine-and-stomach-adjusting fruit-and-vegetable probiotic product and a preparing method thereof.The preparing method includes the steps that fruits and vegetables such as cumquat, hawthorn, apple, lemon, banana, pineapple, grapes, pears, sea buckthorn fruits, kiwi fruits, pumpkin, white radish and carrot with the effects of adjusting the intestine and the stomach and promoting digestion are used, powdered sugar in the fruits and the vegetables is fully converted into probiotic thalluses and metabolic products with the two-stage fermentation method, and meanwhile a large quantity of vitamins, microelements and metabolic organic acids in the fruits and the vegetables are reserved; the concentration of the organic acids is adjusted through calcium carbonate to be converted into organic calcium, intestinal absorption can be achieved, and the acor taste is eliminated; as xylitol and prebiotics are adopted to adjust the sweet taste, calcium absorption can be enhanced, and in-vivo probiotic proliferation and the exogenous probiotic planting effect are enhanced.By means of the product, digestion of abdominal-distension persons which are poor in gastric motility is promoted, intestinal flora can be adjusted, calcium daily needed by the human body can be effectively replenished, and if the digestion-promoting and intestine-and-stomach-adjusting fruit-and-vegetable probiotic product is eaten for a long time, the immunity of the human body can be enhanced.

The invention relates to a broad-spectrum attractant for needle leave wood pests, which is characterized by consisting of an active composition and solvent absolute ethyl alcohol. The active composition consists of alpha-pinene, beta-pinene, camphene, limonene, isolongifolenone, trans-carypohyllene, turpentine, terpinolene and beta-phellandrene. The active composition contains alpha-pinene 40%-50%, beta-pinene 20%-25%, camphene 2.5%-3.2%, limonene 11%-13%, isolongifolenone 4.5%-5.2%, trans-carypohyllene 5%-6%, turpentine 2.5%-3.5%, terpinolene 2.5%-3.5% and beta-phellandrene 0.8%-1.2%. The broad-spectrum attractant has extremely strong luring effect to monochamus alternatus adults, has high luring effect to hyposipalus gigas Linnaeus, shirahoshizo patruelis and xyleborus mutilatus blandford, has good luring effect to more than 20 varieties of pests of coleopteran, is few in using amount, remarkable in luring effect, low in cost and broad-spectrum.

The invention provides a method for extracting active ingredients from tangerine seeds. The method comprises the following steps of: crushing the tangerine seeds, performing supercritical extraction of tangerine oil, performing immersion and homogenizing treatment on oil-removed tangerine seed residue, performing centrifugal separation on the homogenate, performing ultra-filtration treatment on supernate A, and performing affinity chromatography on filtrate and the like to finally obtain limonin with high purity and other active ingredients such as acidic limonin aglycon, nomilin, neutral limonin aglycon (containing no limonin or nomilin) and the like extracted from the waste tangerine seeds so as to realize comprehensive utilization of a tangerine seed resource. The method has high yield, the consumption of an organic solvent is greatly reduced in the extraction process, the flammable and combustible problems caused by extraction with the organic solvent are avoided, the production cost is reduced, and the environmental pollution is reduced.

The invention belongs to the technical field of corrosion prevention, and discloses a composite plant extract with antiseptic and bacteriostatic functions, which comprises the following components: cinnamon extract, clove extract and lemon extract. Wherein the lemon extract contains limonene; the compound plant extract can also comprise at least one of turmeric extract, orange peel extract or staranise extract. The composite plant extract comprises cinnamon extract, clove extract and lemon extract at the same time, has a broad-spectrum antibacterial effect, and has a very good sterilization or inhibition effect on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, candida albicans, aspergillus niger and bacillus cereus; the addition amount of the composite plant extract in food and daily chemical products is 0.04-0.15% in percentage by mass, the addition amount is small, the composite plant extract also has very good antibacterial and preservative effects, and the use cost is greatly reduced.

The invention discloses a method for extracting limonin analogues from tangerine seeds. The method comprises the following steps: firstly, obtaining raw materials, wherein the raw materials are subjected to ultrasonic extraction with 90% ethanol for 1 hour to obtain an extract, the extract is directly detected, the content of limonin is required to be equal to or greater than 0.6% and the content of nomilin is equal to or greater than 0.3%; secondly, breaking hulls of the raw materials and pulverizing until the hulls of the raw materials are completely broken; thirdly, extracting the raw materials with 90% ethanol for three times and 1.5 hours each time and ultimately controlling that the active ingredients in the residue account for no more than 3% of the raw material total target, wherein the ratio of material to solvent is 1 to 4 to 4 to 3; fourthly, concentrating the extract until the degree of alcohol is 20%, standing for 12 hours and removing floating oil on the surface of the liquid; fifthly, continuously recovering the solvent from a concentrated solution, controlling the extraction specific gravity to be 1.03+/-0.1, standing and crystallizing for 12 hours; sixthly, extracting an upper layer supernatant, filtering the lower layer precipitate with filter cloth and centrifuging; and seventhly, after centrifuging, carrying out coarse crystallization and vacuum drying at a temperature of less than 80 DEG C.

The invention provides a compound preservative for fruits. The preservative for the fruits is composed of an antibacterial preservative solution and a waxy mixed solution. The antibacterial preservative solution is prepared by an antibacterial preservative composition, acetic acid- chitosan, galactomannan, pullulan and other components; the waxy mixed solution is prepared by sodium citrate, sodiumphosphate, cyclodextrin, palm wax, trehalose and other components; the antibacterial preservative composition comprises betaine or betaine hydrochloride, beta-aminobutyric acid, glycerol monocaprylate, gallic acid, thyme essential oil or folium artemisiae argyi essential oil, aloe-emodin and limonene. The preservative for the fruits contains the low content of a waxy component, the waxy componenthas low direct contact performance with the fruits and is easily removed after being attached to the fruit surfaces, and no toxic chemical residue exists.

An aquatic emulsion of natural pynethrin for preventing and eliminating the indoor pests (fly, mosquito, etc) and agricultural pests (aphis, locust, etc) contains proportionally natural pyrethrin, limonene, rosemary, tween, beta-cyclodextrin, non-ionic emulsifier and co-solvent. It has very low toxin and almost no by-effect.

The invention belongs to the technical field of health-care products and particularly relates to a composition containing kudzuvine roots as well as a composition extraction device and method. The composition is prepared from components of raw materials in parts by weight as follows: 20-60 parts of the kudzuvine roots, 20-60 parts of pine pollen, 0-20 parts of gastrodia elata, 0-20 parts of polygonatum, 0-15 parts of polygonatum odoratum, 0-20 parts of pseudo-ginseng, 8-30 parts of lemons and 10-30 parts of honey. The extraction device comprises a microwave extraction chamber and an ultrasonic wave extraction chamber. The extraction method comprises freeze drying and smashing treatment of the kudzuvine roots, freeze drying treatment after fixation of the gastrodia elata and freeze drying treatment of the honey and the lemons. Efficacy components are reserved sufficiently, and absorption by a human body is facilitated.

The invention discloses a cold-resist gene PtrERF109 of Poncirus trifoliata and application thereof in cold resistance genetic improvement of plants. The PtrERF109 gene is a transcription factor isolated and cloned from extremely-cold-resist Poncirus trifoliata, and the sequence is as shown in SEQ. ID NO. 1. Overexpression RNAi vectors of the gene are constructed, and the vectors of the PtrERF109gene are respectively introduced into tobacco, lemon and Poncirus trifoliata by Agrobacterium-mediated genetic transformation to obtain transgenic plants. Verification of biological functions of the obtained transgenic plants indicates that the cloned PtrERF109 gene has the function of improving the cold resistance of the plants. The discovery of the gene provides new genetic resources for anti-reverse molecular design and breeding of the plants, and provides new genetic resources for the implementation of green agriculture and water-saving agriculture. The development and utilization of the genetic resources are conducive to reducing agricultural production costs and achieving environmental friendliness.

The invention discloses a method for debittering citrus juice. The method comprises the following steps of: a) destroying a colloid system in the juice by a hydrolysis method or a pH value control method; and b) centrifugally separating bitter substances out under the low temperature condition, and thus obtaining the juice with low bitter substance content. According to the hydrolysis method, pectin or plant fibers in the juice is or are hydrolyzed by pectinase or cellulose. According to the pH value control method, the pH value of the juice is reduced by adding an acid solution. According tothe method, the colloid system of the juice is destroyed, and the bitter substances, in particular limonin type bitter substances, are separated from the juice by a low temperature centrifugal method, so that a debittering effect is achieved.

The invention discloses a acetobacter pasteurium and its application method in producing citrus vinegars and resulting citrus vinegar products. The invention provides acetobacter pasteurium named LSYY001.2, preserved in CGMCC on 14th June, 2010, preservation number: CGMCC No.4954. Method of the invention for producing citrus vinegar comprises: selecting materials, peeling citruses, removing segment membranes, crushing, filtering, adding yeasts for alcoholic fermentation, adding LSYY001.2 for acetic acid fermentation, then filtering. The acetobacter pasteurium provided by the invention not only ferments alcohol for producing acetic acid, but simultaneously produces iso-limonin hydrolase, thus realizing removing bitter of the citrus vinegar.

The invention discloses a biogenic herbicide which is characterized by being prepared from, by mass, 15-25 parts of artemisia scoparia, 10-15 parts of fragrant eupatorium herb, 10-20 parts of coptis chinensis, 10-20 parts of melia azedarach bark, 15-25 parts of celastrus angulatus, 10-20 parts of dicranopteris pedata, 20-30 parts of lemons, 10-20 parts of green plums and 1-3 parts of a penetrating agent.The materials are smashed, extracted, mixed and purified to prepare the biogenic herbicide.The biogenic herbicide is good in drug type.Through medicine effect comparison, the biogenic herbicide has a better prevention effect than a traditional herbicide and is free of toxin and harm, little in environment pollution and capable of effectively guaranteeing safety of crops, and accordingly it is guaranteed that people and animals cannot be harmed by absorbing chemical matter from the crops.

The invention discloses an extraction method of limonin. The extraction method comprises the following steps: pretreatment: adding lemon seeds and lemon peel residues into water, carrying out soaking,and adding a pretreatment enzyme to decompose impurities; extraction: adding an extracting enzyme into a pretreatment product for enzymolysis, adding acid for acidolysis, performing solid-liquid separation, removing solid residues, and reserving liquid to obtain a primary product; removal of pectin: carrying out vacuum concentration on the primary product, adding alcohol for alcohol precipitation, separating a floccule precipitate, and reserving liquid; and purification: carrying out extracting at first, and then conducting separating by using macroporous resin. The extraction method disclosed by the invention is reasonable in design, all the steps are associated with one another, impurities are removed one by one, and extraction and macroporous resin separation are combined for use; andextraction is carried out at first and then macroporous resin separation is carried out, and the advantages of two separation means are combined, so separation efficiency is high, and product purity is high.

The invention discloses a yarrowia lipolytica genetically engineered bacterium for producing limonene. A method comprises the following steps: introducing an optimized xylose reductase gene XR, an optimized xylitol dehydrogenase gene XDH and an optimized xylose assimilation enzyme gene XK into chromosomes of uracil and leucine auxotrophic yarrowia lipolytica to construct a yarrowia lipolytica genetically engineered bacterium YBX06; transforming a recombinant vector containing two copy-optimized limonene synthase genes LS and one copy-optimized neroli diphosphate synthase 1 gene NDPS1 into theyarrowia lipolytica genetically engineered bacterium YBX06, and performing construction to obtain the yarrowia lipolytica genetically engineered bacterium YBX07. The yarrowia lipolytica genetically engineered bacterium constructed by the invention can utilize lignocellulose hydrolysate as a raw material and synchronously utilize xylose and glucose as a mixed carbon source to produce the limonene through biological fermentation, so that the yarrowia lipolytica genetically engineered bacterium has a good application prospect.

The invention discloses an extraction method for limonin. The extraction method includes the following steps of (1) raw material extracting in a soaked mode or juicing, wherein a raw material is directly soaked in water or directly juiced through a squeezer to obtain an extraction solution; (2) metal salt adding and pH adjusting, wherein sulfate solid matter or a sulfate saturated solution is added to the extraction solution, and the pH is adjusted to be 3-7; (3) heating and temperature keeping, wherein the solution is heated to 20 DEG C-100 DEG C, and the temperature is kept for 10 min-110 min; (4) centrifuging, the solution is centrifuged for 10 min at the rotating speed of 2500*g to obtain a limonin precipitate. Compared with a traditional method, the raw material can be directly soaked to obtain the extraction solution or directly juiced to obtain a squeezed solution without being dried by adopting the method, most of the limonin in the water is recovered through a precipitation method, complex equipment is not needed, and the extraction method is simple and convenient to operate and low in technological cost.

The invention relates to the field of essence, and particularly discloses oriental flower flavored essence and a preparation method thereof. The oriental flowery flavor essence consists of a main flavor, a modifier, a mixing agent, a fixative and a solvent. The main flavor is prepared from the following components: linalool, benzyl alcohol, phenethyl alcohol, benzyl acetate, methyl anthranilate, methyl benzoate, methyl salicylate, iso-methyl ionone, alpha-hexylcinnamic aldehyde, anisic aldehyde, hydroxycitronellal, undecan-4-olide, specially pure vanillin, ethyl maltol, eugenol and limonene. The modifier is prepared from the following components: cinnamic alcohol, rose ether and cedar wood oil. The oriental flower flavored essence has the advantages that the oriental flower flavored essence has a comfortable and natural feeling while generating complicated fragrance change.

The invention provides Acetobacter malorum ZY.C4 with a collection number of CGMCC NO:8692. The strain is quick in acid production rate and high in ester yield, has relatively good temperature resistance and alcohol resistance, is stable in performance after five times of passage, and is low in acetic acid decomposition speed; and moreover, the strain can generate a limonin catabolic enzyme to decompose limonin in an orange juice, thereby removing bitterness from an orange fruit vinegar. The orange fruit vinegar brewed by using the strain is soft in acidity, strong in fruity flavor, rich in nutrition and good in mouthfeel.

The invention provides an application of pinus massoniana alpha-pinene synthetase to preparation of terpene type compounds and products including the terpene type compounds, and relates to the technical field of plant molecule biology. Appha-pinene is an important defensive substance on the secondary metabolism level of the pinus massoniana, and the synthesis of the alpha-pinene is adjusted and controlled by alpha-pinene synthetase. The inventor of the invention finds that the pinus massoniana alpha-pinene synthetase can also catalyze and synthesize beta-pinene, beta-laurene and D-limonene, and is synthetase capable of controlling productive substances, so that the invention provides the application of the pinus massoniana alpha-pinene synthetase to preparation of at least one of the beta-pinene, beta-laurene and D-limonene. A process of synthesizing the terpene type compounds by using the pinus massoniana alpha-pinene synthetase as a catalysis substrate is close to a biological natural synthesis process, the synthesis efficiency is high, the impurity content of intermediate products and other non-target products is low, and pollution of chemical substances in traditional chemicalsynthesis can be avoided.

The invention discloses a high-yield citrus limon planting method and relates to the technical field of citrus limon planting. The high-yield citrus limon planting method comprises the specific steps of planting land selection and land preparation, planting and complementary planting, orchard management and disease and pest control. By means of the high-yield citrus limon planting method, the survival rate is high; citrus limon has the beneficial effects of being great in adaptability and high in yield; economic output of a citrus limon orchard is high; it is helpful to promote citrus limon industrialization development of China; the fruit self-sufficiency rate of China can be increased; and the method is a strategic measure for cultivating and expanding the citrus limon wood industry and guaranteeing lemon supply of China.

The subject matter of the invention is a carrier loaded with bioactive substances, wherein the carrier is carbonate substituted hydroxyapatite and

• • the bioactive substances are an ion and a plant extract, wherein said ion is selected from the group consisting of Cu, Zn and a combination thereof, and
  • wherein the plant extract consists of mint extract, rosemary extract, Ocimum basilicum extract, lemon extract, garlic extract, clove; extract, thyme extract and cinnamon extract.

The invention provides a method for testing young-citrus-fruit-originated volatile matters having effects on adult Bactrocera minax. According to the results of the testing method, the young-citrus-fruit-originated volatile matters including limonene, 4-terpene alcohol, nonanal and citral have behavioral reaction effects on adult Bactrocera minax; limonene, 4-terpene alcohol and citral all have effects on male citrus Bactrocera minax; and limonene and nonanal both have effects on female citrus Bactrocera minax. It is confirmed for the first time that the young-citrus-fruit-originated volatilematters having effects on male and female adults of Bactrocera minax include limonene, 4-terpene alcohol, nonanal and citral, and the young-citrus-fruit-originated volatile matters can be further applied to research and development of attractants and repellents and play a positive role in pollution-free prevention and treatment in citrus production areas.