135 results about "Citrus x limonia" patented technology

Disclosed is a composition comprising at least two of the following ingredients: Magnolia extract, honokiol, humulus lupulus extract, hesperidin methyl chalcone, gotu kola, dipeptide valyl-tryptophane, palmitoyl tetrapeptide-3, corylus avellana bud extract, centella asiatica extract, cucumis sativa extract, morus alba extract, hibiscus sabdariffa flower extract, vitis vinifera extract, ascorbyl glucoside, citrus medica limonum extract, avena sativa kernel extract, hydrolyzed soy protein, aniseed myrtle extract, tasmania lanceolata leaf extract, artemisia abrotanum extract, or citrus grandis fruit extract or any combination thereof. Also disclosed are methods of treating skin conditions by topically applying the composition to skin.

The invention discloses a digestion-promoting and intestine-and-stomach-adjusting fruit-and-vegetable probiotic product and a preparing method thereof.The preparing method includes the steps that fruits and vegetables such as cumquat, hawthorn, apple, lemon, banana, pineapple, grapes, pears, sea buckthorn fruits, kiwi fruits, pumpkin, white radish and carrot with the effects of adjusting the intestine and the stomach and promoting digestion are used, powdered sugar in the fruits and the vegetables is fully converted into probiotic thalluses and metabolic products with the two-stage fermentation method, and meanwhile a large quantity of vitamins, microelements and metabolic organic acids in the fruits and the vegetables are reserved; the concentration of the organic acids is adjusted through calcium carbonate to be converted into organic calcium, intestinal absorption can be achieved, and the acor taste is eliminated; as xylitol and prebiotics are adopted to adjust the sweet taste, calcium absorption can be enhanced, and in-vivo probiotic proliferation and the exogenous probiotic planting effect are enhanced.By means of the product, digestion of abdominal-distension persons which are poor in gastric motility is promoted, intestinal flora can be adjusted, calcium daily needed by the human body can be effectively replenished, and if the digestion-promoting and intestine-and-stomach-adjusting fruit-and-vegetable probiotic product is eaten for a long time, the immunity of the human body can be enhanced.

The invention relates to a broad-spectrum attractant for needle leave wood pests, which is characterized by consisting of an active composition and solvent absolute ethyl alcohol. The active composition consists of alpha-pinene, beta-pinene, camphene, limonene, isolongifolenone, trans-carypohyllene, turpentine, terpinolene and beta-phellandrene. The active composition contains alpha-pinene 40%-50%, beta-pinene 20%-25%, camphene 2.5%-3.2%, limonene 11%-13%, isolongifolenone 4.5%-5.2%, trans-carypohyllene 5%-6%, turpentine 2.5%-3.5%, terpinolene 2.5%-3.5% and beta-phellandrene 0.8%-1.2%. The broad-spectrum attractant has extremely strong luring effect to monochamus alternatus adults, has high luring effect to hyposipalus gigas Linnaeus, shirahoshizo patruelis and xyleborus mutilatus blandford, has good luring effect to more than 20 varieties of pests of coleopteran, is few in using amount, remarkable in luring effect, low in cost and broad-spectrum.

The invention provides a method for extracting active ingredients from tangerine seeds. The method comprises the following steps of: crushing the tangerine seeds, performing supercritical extraction of tangerine oil, performing immersion and homogenizing treatment on oil-removed tangerine seed residue, performing centrifugal separation on the homogenate, performing ultra-filtration treatment on supernate A, and performing affinity chromatography on filtrate and the like to finally obtain limonin with high purity and other active ingredients such as acidic limonin aglycon, nomilin, neutral limonin aglycon (containing no limonin or nomilin) and the like extracted from the waste tangerine seeds so as to realize comprehensive utilization of a tangerine seed resource. The method has high yield, the consumption of an organic solvent is greatly reduced in the extraction process, the flammable and combustible problems caused by extraction with the organic solvent are avoided, the production cost is reduced, and the environmental pollution is reduced.

The invention belongs to the technical field of corrosion prevention, and discloses a composite plant extract with antiseptic and bacteriostatic functions, which comprises the following components: cinnamon extract, clove extract and lemon extract. Wherein the lemon extract contains limonene; the compound plant extract can also comprise at least one of turmeric extract, orange peel extract or staranise extract. The composite plant extract comprises cinnamon extract, clove extract and lemon extract at the same time, has a broad-spectrum antibacterial effect, and has a very good sterilization or inhibition effect on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, candida albicans, aspergillus niger and bacillus cereus; the addition amount of the composite plant extract in food and daily chemical products is 0.04-0.15% in percentage by mass, the addition amount is small, the composite plant extract also has very good antibacterial and preservative effects, and the use cost is greatly reduced.

The invention relates to the technical field of plant separation and extraction, and in particularly discloses a method of combined extraction of essential oil, pectin, hesperidin, synephrine and limonin from citrus. The method comprises the following steps: inactivating the citrus, buffing and performing enzymolysis to obtain essential oil, a water phase and skin residues; separating the water phase through strong acidic cation-exchange resin to collect effusive liquid, and eluting to obtain synephrine; separating the effusive liquid using ultra-filtration method to obtain pectin and liquid passing through the ultra-filtration film; refluxing and extracting the liquid passing through the ultra-filtration film using ethanol to obtain supernatant and filter residue; crystallizing, and freeze-drying the filter residues to obtain aurantiamarin; separating the supernatant using macroporous resin, and eluting using ethanol to obtain limonin. The method is used for processing fresh citrus to co-produce the citrus pericarp essential oil, pectin, hesperidin, synephrine and limonin; the utilization rate of the resource is improved, the environment pollution is reduced, and the economic and social benefits are notable.

The invention belongs to the field of the extraction of natural medicines and particularly relates to a limonin analogue and a separation and purification method thereof. The invention aims to solve the technical problem of providing a fire-new separation and purification method of a limonin analogue. According to the separation and purification method disclosed by the invention, the medicinal materials of rue family citrus plants, such as tangerine seeds, pummelo seeds, immature bitter oranges, tangerine peels and the like, are used as raw materials, 40 to 50 percent of ethanol is used as a solvent for extraction, an extract is separated and purified by adopting HPD-722 type macroporous resin, the content of the limonin analogue in obtained solid matters is greater than 80 percent, the content of limonin is greater than 20 percent, and the content of nomilin is greater than 40 percent. The method has high extraction efficiency, little reagent consumption and little pollution and is simple to operate.

The invention discloses a method for extracting limonin analogues from tangerine seeds. The method comprises the following steps: firstly, obtaining raw materials, wherein the raw materials are subjected to ultrasonic extraction with 90% ethanol for 1 hour to obtain an extract, the extract is directly detected, the content of limonin is required to be equal to or greater than 0.6% and the content of nomilin is equal to or greater than 0.3%; secondly, breaking hulls of the raw materials and pulverizing until the hulls of the raw materials are completely broken; thirdly, extracting the raw materials with 90% ethanol for three times and 1.5 hours each time and ultimately controlling that the active ingredients in the residue account for no more than 3% of the raw material total target, wherein the ratio of material to solvent is 1 to 4 to 4 to 3; fourthly, concentrating the extract until the degree of alcohol is 20%, standing for 12 hours and removing floating oil on the surface of the liquid; fifthly, continuously recovering the solvent from a concentrated solution, controlling the extraction specific gravity to be 1.03+ / -0.1, standing and crystallizing for 12 hours; sixthly, extracting an upper layer supernatant, filtering the lower layer precipitate with filter cloth and centrifuging; and seventhly, after centrifuging, carrying out coarse crystallization and vacuum drying at a temperature of less than 80 DEG C.

The invention provides a compound preservative for fruits. The preservative for the fruits is composed of an antibacterial preservative solution and a waxy mixed solution. The antibacterial preservative solution is prepared by an antibacterial preservative composition, acetic acid- chitosan, galactomannan, pullulan and other components; the waxy mixed solution is prepared by sodium citrate, sodiumphosphate, cyclodextrin, palm wax, trehalose and other components; the antibacterial preservative composition comprises betaine or betaine hydrochloride, beta-aminobutyric acid, glycerol monocaprylate, gallic acid, thyme essential oil or folium artemisiae argyi essential oil, aloe-emodin and limonene. The preservative for the fruits contains the low content of a waxy component, the waxy componenthas low direct contact performance with the fruits and is easily removed after being attached to the fruit surfaces, and no toxic chemical residue exists.

The present invention relates to novel processes for producing ethanol from citrus waste using suitable solvent extractions to reduce the presence of fermentation inhibiting compounds, such as limonene, from citrus waste. The extraction can be performed before or after the polysaccharides present in aqueous mixtures comprising citrus waste are converted into fermentable sugars.

An aquatic emulsion of natural pynethrin for preventing and eliminating the indoor pests (fly, mosquito, etc) and agricultural pests (aphis, locust, etc) contains proportionally natural pyrethrin, limonene, rosemary, tween, beta-cyclodextrin, non-ionic emulsifier and co-solvent. It has very low toxin and almost no by-effect.

The invention discloses a method for removing the bitter taste of lemon juice with a macroporous resin, belonging to the technical field of drink preparation. The method comprises the following steps: selecting ripe and undamaged fresh lemons and washing, peeling and juicing the fresh lemons and filtering the juice to obtain the lemon juice; deactivating the enzyme of the lemon juice at 85-95 DEG C for 8-15 minutes and cooling the lemon juice; soaking the macroporous resin LS-803C in absolute ethyl alcohol, washing the macroporous resin LS-803C with an acid and an alkali and removing the impurities and then packing a column with the macroporous resin LS-803C; and passing the treated lemon juice through a resin column with height-diameter ratio of (5:1)-(20:1) at a flow rate of 2-11BV / h at a room temperature of 20 DEG C, thus obtaining the debitterized lemon juice. The method has the beneficial effects that the rates of removal of bitter substances in the lemon juice, especially naringin and limonin are respectively higher than 90% and 60%; the used resin can be reused, so that the processing cost is relatively low and the method is convenient to operate; the two bitter substances, namely naringin and limonin adsorbed by the resin can be further separated, purified and recycled.

Certain components of citrus fruits and oxidation products of limonene are effective deterrents, repellents and / or spatial inhibitors for mosquitoes and biting midges. The compounds that have been found to be deterrents, repellents and infibitors for mosquitoes and biting midges are neryl acetate, citronellyl acetate, geranyl acetate, hydroxy-p-cymene, citral, α-terpineol, citronellal, linaloyl acetate, citronellol, terpen-4-ol, tetrahydrocarvone, products of oxidized oxidized limonene inclusive of d- and l-carvone, (+) limonene oxide, (−) limonene oxide, cis and tran carveol, a diol and an aldehyde, and mixtures thereof.

The invention belongs to the technical field of health-care products and particularly relates to a composition containing kudzuvine roots as well as a composition extraction device and method. The composition is prepared from components of raw materials in parts by weight as follows: 20-60 parts of the kudzuvine roots, 20-60 parts of pine pollen, 0-20 parts of gastrodia elata, 0-20 parts of polygonatum, 0-15 parts of polygonatum odoratum, 0-20 parts of pseudo-ginseng, 8-30 parts of lemons and 10-30 parts of honey. The extraction device comprises a microwave extraction chamber and an ultrasonic wave extraction chamber. The extraction method comprises freeze drying and smashing treatment of the kudzuvine roots, freeze drying treatment after fixation of the gastrodia elata and freeze drying treatment of the honey and the lemons. Efficacy components are reserved sufficiently, and absorption by a human body is facilitated.

The invention discloses a cold-resist gene PtrERF109 of Poncirus trifoliata and application thereof in cold resistance genetic improvement of plants. The PtrERF109 gene is a transcription factor isolated and cloned from extremely-cold-resist Poncirus trifoliata, and the sequence is as shown in SEQ. ID NO. 1. Overexpression RNAi vectors of the gene are constructed, and the vectors of the PtrERF109gene are respectively introduced into tobacco, lemon and Poncirus trifoliata by Agrobacterium-mediated genetic transformation to obtain transgenic plants. Verification of biological functions of the obtained transgenic plants indicates that the cloned PtrERF109 gene has the function of improving the cold resistance of the plants. The discovery of the gene provides new genetic resources for anti-reverse molecular design and breeding of the plants, and provides new genetic resources for the implementation of green agriculture and water-saving agriculture. The development and utilization of the genetic resources are conducive to reducing agricultural production costs and achieving environmental friendliness.

The invention discloses a method for debittering citrus juice. The method comprises the following steps of: a) destroying a colloid system in the juice by a hydrolysis method or a pH value control method; and b) centrifugally separating bitter substances out under the low temperature condition, and thus obtaining the juice with low bitter substance content. According to the hydrolysis method, pectin or plant fibers in the juice is or are hydrolyzed by pectinase or cellulose. According to the pH value control method, the pH value of the juice is reduced by adding an acid solution. According tothe method, the colloid system of the juice is destroyed, and the bitter substances, in particular limonin type bitter substances, are separated from the juice by a low temperature centrifugal method, so that a debittering effect is achieved.

The invention discloses a acetobacter pasteurium and its application method in producing citrus vinegars and resulting citrus vinegar products. The invention provides acetobacter pasteurium named LSYY001.2, preserved in CGMCC on 14th June, 2010, preservation number: CGMCC No.4954. Method of the invention for producing citrus vinegar comprises: selecting materials, peeling citruses, removing segment membranes, crushing, filtering, adding yeasts for alcoholic fermentation, adding LSYY001.2 for acetic acid fermentation, then filtering. The acetobacter pasteurium provided by the invention not only ferments alcohol for producing acetic acid, but simultaneously produces iso-limonin hydrolase, thus realizing removing bitter of the citrus vinegar.

The present invention relates to an antimicrobial pesticide composition. The composition comprises limonene, linalool, beta-pinene, alpha-terpineol, citral, myrcene, and hexanal. The invention not only provides a new way for development and utilization of active substances in citrus, but also provides a basis for the citrus active substances used in research and development of antimicrobial pesticides.

The invention relates to an injurious insect trapping adhesive.The injurious insect trapping adhesive is prepared from, by weight, 95% of insect sticking glue, 0.55% of limonene, 0.45% of pinene, 2% of plant source pheromones and 2% of insect sex pheromones.The trapping adhesive is strong in insect trapping capability and attracting capability and long in lasting period.

Plumbaginaceae is a highly stress-tolerant angiosperm family consisting of approximately 15 genera of which the genus Limonium is the largest. Many species of Limonium (also called sea lavender and statice) are of commercial importance in ornamental horticulture, especially for cut flowers. It is taught herein that it is possible to generate Limonium plants via somatic embryogenesis and in turn provide a means for repetitive embryogenesis. This repetitive property allows perpetuation of the embryogenic state indefinitely and thereby produce large numbers of Limonium embryos for diverse goals such as mass propagation, production of valuable compounds and for the production of transgenic plants.

The invention discloses a preparation method of citrus essential oil series products. The method comprises the following steps: 1) citrus peel pretreatment: cleaning fresh citrus peel, removing impurities, airing and dicing; 2) squeezing and extracting: performing cold pressing on the orange peel to extract oil; 3) extraction by an ultrasonic-distillation method: firstly treating by an ultrasonicdevice, and then distilling to extract essential oil; 4) dehydration and impurity removal: adding a drying agent for dehydration; and 5) low temperature concentrated distillation: carrying out concentration and terpene removal with a rotary distillation device to obtain concentrated citrus essential oil and limonene. According to the method, the extraction rate of the citrus essential oil is increased, the quality of the citrus essential oil is improved, and the method is low in cost, easy to operate and suitable for industrial large-scale production.

The invention discloses a biogenic herbicide which is characterized by being prepared from, by mass, 15-25 parts of artemisia scoparia, 10-15 parts of fragrant eupatorium herb, 10-20 parts of coptis chinensis, 10-20 parts of melia azedarach bark, 15-25 parts of celastrus angulatus, 10-20 parts of dicranopteris pedata, 20-30 parts of lemons, 10-20 parts of green plums and 1-3 parts of a penetrating agent.The materials are smashed, extracted, mixed and purified to prepare the biogenic herbicide.The biogenic herbicide is good in drug type.Through medicine effect comparison, the biogenic herbicide has a better prevention effect than a traditional herbicide and is free of toxin and harm, little in environment pollution and capable of effectively guaranteeing safety of crops, and accordingly it is guaranteed that people and animals cannot be harmed by absorbing chemical matter from the crops.

The invention discloses an extraction method of limonin. The extraction method comprises the following steps: pretreatment: adding lemon seeds and lemon peel residues into water, carrying out soaking,and adding a pretreatment enzyme to decompose impurities; extraction: adding an extracting enzyme into a pretreatment product for enzymolysis, adding acid for acidolysis, performing solid-liquid separation, removing solid residues, and reserving liquid to obtain a primary product; removal of pectin: carrying out vacuum concentration on the primary product, adding alcohol for alcohol precipitation, separating a floccule precipitate, and reserving liquid; and purification: carrying out extracting at first, and then conducting separating by using macroporous resin. The extraction method disclosed by the invention is reasonable in design, all the steps are associated with one another, impurities are removed one by one, and extraction and macroporous resin separation are combined for use; andextraction is carried out at first and then macroporous resin separation is carried out, and the advantages of two separation means are combined, so separation efficiency is high, and product purity is high.

The invention discloses a yarrowia lipolytica genetically engineered bacterium for producing limonene. A method comprises the following steps: introducing an optimized xylose reductase gene XR, an optimized xylitol dehydrogenase gene XDH and an optimized xylose assimilation enzyme gene XK into chromosomes of uracil and leucine auxotrophic yarrowia lipolytica to construct a yarrowia lipolytica genetically engineered bacterium YBX06; transforming a recombinant vector containing two copy-optimized limonene synthase genes LS and one copy-optimized neroli diphosphate synthase 1 gene NDPS1 into theyarrowia lipolytica genetically engineered bacterium YBX06, and performing construction to obtain the yarrowia lipolytica genetically engineered bacterium YBX07. The yarrowia lipolytica genetically engineered bacterium constructed by the invention can utilize lignocellulose hydrolysate as a raw material and synchronously utilize xylose and glucose as a mixed carbon source to produce the limonene through biological fermentation, so that the yarrowia lipolytica genetically engineered bacterium has a good application prospect.

The invention discloses an extraction method for limonin. The extraction method includes the following steps of (1) raw material extracting in a soaked mode or juicing, wherein a raw material is directly soaked in water or directly juiced through a squeezer to obtain an extraction solution; (2) metal salt adding and pH adjusting, wherein sulfate solid matter or a sulfate saturated solution is added to the extraction solution, and the pH is adjusted to be 3-7; (3) heating and temperature keeping, wherein the solution is heated to 20 DEG C-100 DEG C, and the temperature is kept for 10 min-110 min; (4) centrifuging, the solution is centrifuged for 10 min at the rotating speed of 2500*g to obtain a limonin precipitate. Compared with a traditional method, the raw material can be directly soaked to obtain the extraction solution or directly juiced to obtain a squeezed solution without being dried by adopting the method, most of the limonin in the water is recovered through a precipitation method, complex equipment is not needed, and the extraction method is simple and convenient to operate and low in technological cost.

The invention relates to the field of essence, and particularly discloses oriental flower flavored essence and a preparation method thereof. The oriental flowery flavor essence consists of a main flavor, a modifier, a mixing agent, a fixative and a solvent. The main flavor is prepared from the following components: linalool, benzyl alcohol, phenethyl alcohol, benzyl acetate, methyl anthranilate, methyl benzoate, methyl salicylate, iso-methyl ionone, alpha-hexylcinnamic aldehyde, anisic aldehyde, hydroxycitronellal, undecan-4-olide, specially pure vanillin, ethyl maltol, eugenol and limonene. The modifier is prepared from the following components: cinnamic alcohol, rose ether and cedar wood oil. The oriental flower flavored essence has the advantages that the oriental flower flavored essence has a comfortable and natural feeling while generating complicated fragrance change.

The invention provides Acetobacter malorum ZY.C4 with a collection number of CGMCC NO:8692. The strain is quick in acid production rate and high in ester yield, has relatively good temperature resistance and alcohol resistance, is stable in performance after five times of passage, and is low in acetic acid decomposition speed; and moreover, the strain can generate a limonin catabolic enzyme to decompose limonin in an orange juice, thereby removing bitterness from an orange fruit vinegar. The orange fruit vinegar brewed by using the strain is soft in acidity, strong in fruity flavor, rich in nutrition and good in mouthfeel.

The invention relates to a detection method for limonins components in a szechwan Chinaberry fruit medicinal material and an extractive of the szechwan Chinaberry fruit medicinal material. The detection method comprises the following steps of: carrying out quantitative analysis on 1-deacetyl nimbolinin B and carrying out qualitative and quantitative analysis on one or more components of nimbolinin A, nimbolinin B, melia toosendan linin and toosendanin. The detection method particularly comprises the following steps of: adopting the 1-deacetyl nimbolinin B and other component comparison products of the type and adopting a high performance liquid-evaporative light-scattering detector to carry out the quantitative analysis on the 1-deacetyl nimbolinin B and the qualitative and quantitative analysis on the other components; carrying out thin-layer identification; adopting a color developing method to determine the content of total triterpenes. According to the detection method provided by the invention, the 1-deacetyl nimbolinin B and the components of the same type are used as the comparison products to systematically establish a nimbolinins component qualitative and quantitative analysis method for the first time; the method is scientific and reasonable, and is simple and feasible; the detection method is suitable for the szechwan Chinaberry fruit medicinal material and a processed product and the quality control of the nimbolinin type limonins components in congener fruit extractives.

The invention relates to the field of acne-removing products, and particularly discloses an acne-removing emulsion and a preparation method thereof. The acne-cleaning emulsion is prepared from the following 100 parts by weight of raw materials: 2-8 parts of dioctyl ether, 2-8 parts of coconut oil alcohol-octoate / caprate, 2-8 parts of butyrospermum parkii butter, 1-5 parts of glycerol, 0.1-4 parts of behenyl alcohol, 0.1-3 parts of glyceryl behenate, 0-2 parts of lecithin, 0-1 part of vitamin E, 0-1 part of sunflower seed oil, 0.1-2 parts of cetyl alcohol, 0.1-2 parts of xanthan gum and 0-1 part of polylysine. 0-2 parts of lemon pekoe leaf extract, 0-1 part of phenoxyethanol, 0-1 part of ethylhexylglycerin and the balance of deionized water. The acne-removing emulsion has a good acne-removing effect, also has high mildness, is low in irritation, and is not liable to cause allergy after being used for a long time.