Digestion-promoting and intestine-and-stomach-adjusting fruit-and-vegetable probiotic product and preparing method thereof
A technology of vegetable probiotics and gastrointestinal fruit, which is applied in the field of promoting digestion and adjusting gastrointestinal fruit and vegetable probiotic products and its preparation, can solve the problems of denaturation and inactivation, ignore the natural flora, reduce the reproduction of harmful bacteria, etc., and achieve the effect of nutritional balance
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Embodiment 1
[0065] The best recommended program for children aged 4-12:
[0066](1) Strain preparation method: Bifidobacterium adolescentis CICC6180, Bifidobacterium infantis CICC6069, Bifidobacterium animalis CICC6165 are respectively inoculated in 150mLTPY liquid medium according to the inoculum amount of 5% (w / w), strict anaerobic static Cultivate for 24h to 36h, centrifuge at 10°C and 4000rpm for 5min, add 50mL of sterile saline to wash the sludge, collect the precipitate twice, mix the precipitate according to the mass ratio of 1:1:1, take 5g of the sludge and inoculate 2kg of fruit and vegetable culture In the base; Lactobacillus paracasei CICC6236, Lactobacillus acidophilus CICC6088, Lactobacillus plantarum CICC6238, Lactobacillus rhamnosus CICC6133 were respectively inoculated in 100mL lactic acid bacteria medium, yeast strain CICC1249 was inserted into 100mL yeast medium, anaerobic After static culture for 24 hours, centrifuge at 4000rpm at 10°C for 5 minutes, add 50mL of sterile...
Embodiment 2
[0074] The best recommended solution for middle-aged and elderly people:
[0075] (1) Strain preparation method: inoculate Bifidobacterium longum CICC6186, Bifidobacterium breve CICC6185, Bifidobacterium bifidum CICC6170, Bifidobacterium juvenile CICC6180, Bifidobacterium animalis CICC6165 into 200mLTPY liquid according to the inoculum amount of 5%. In the culture medium, strict anaerobic static culture for 24h ~ 36h, centrifuge at 4000rpm at 10°C for 5min, add 50mL sterile normal saline to wash the sludge, collect the precipitate twice, and divide the precipitate according to the mass ratio of 1:1:1:1:1 After mixing, take 10g of bacteria slime and inoculate it into 4kg of fruit and vegetable medium; inoculate Lactobacillus paracasei CICC6236, Lactobacillus acidophilus CICC6088, and Lactobacillus rhamnosus CICC6133 into 100mL of lactic acid bacteria medium respectively, and yeast strain CICC1249 into 100mL In the yeast medium, after anaerobic static culture for 24 hours, centr...
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