73 results about "Limonoid" patented technology

The present invention describes compositions and methods for treating, preventing and improving the appearance of skin, particularly, treating, preventing, ameliorating, reducing and / or eliminating fine lines and / or wrinkles of skin, wherein the compositions include limonoid constituents which inhibit acetylcholine release at neuromuscular junctions of skeletal muscle so as to relax the muscles involved with wrinkling, folding and creasing of skin, e.g., facial movement and expression. The limonoids preferably include the plant alkaloids toosendanin and azadirachtin. The compositions, which also are used to treat hyperhidrosis, are preferably applied to the skin, or are delivered by directed means to a site in need thereof.

Compositions and methods for the prevention and treatment of neoplastic diseases are described. Individuals at a high risk of developing or having neoplasia undergoing conventional therapies may be treated with an effective dose of triterpene derivatives in limonoids, polyphenolic flavonoid compounds, tocotrienols or a combination of these agents.

/ Composition and methods for the prevention and treatment of hypercholesterolemia, hyperlipidemia and atherosclerosis are described. Individuals at a high risk of developing or having hypercholesterolemia and atherosclerosis undergoing conventional therapies may be treated with an effective dose of triperpene derivatives in limonoids, polyphenolic flavonoid compounds, tocotrienols or a combination of these agents.

Compositions and methods for the prevention and treatment of neoplastic diseases and hypercholesterolemia are described. Individuals at a high risk of developing or having neoplasia or hypercholesterolemia undergoing conventional therapies may be treated with an effective dose of triterpene derivatives in citrus limonoids, polyphenolic flavonoid citrus compounds, tocotrienols or a combination of these agents.

The invention belongs to the technical field of natural product extraction from oranges, and specifically relates to a method for continuously extracting natural activated products such as synephrine, hesperidin and limonin from physiological orange drops. The method comprises the following steps: using orange drops which are dried and crushed into 20-100 meshes as raw materials, adding 50-90% ethanol according to a weight / volume ratio of (1:10)-(1:50), extracting under reflux at 40-80 DEG C for 1-3 hours, filtering, recovering ethanol from the filtrate, and obtaining a concentrated solution for later use; adding a small number of chloroform crystals into the concentrated solution, adding water for layering, directly heating the water layer to 75 DEG C, filtering, adding 0.1mol / L hydrochloric acid to regulate the pH value to 3-4, and precipitating to obtain a hesperidin crude product; carrying out spin drying on the obtained chloroform layer solution, adding a right number of acetone crystals, and carrying out vacuum drying to obtain a synephrine crude product; and carrying out spin drying on the acetone solution after the crystal filtration, adding dichloromethane for dissolution, filtering, adding isopropanol into the solution according to a volume ratio of 1:2, and crystallizing to obtain a limonin crude product. Qualitative and quantitative analysis are carried out on the three resulting activated products through high-pressure liquid chromatography, and the result shows that the hesperidin has the highest purity (91.41%).

The present invention is directed to compositions and methods for the treatment and / or prevention of metabolic syndrome and its associated conditions, such as insulin resistance, which involve using a combination composition of limonoids, flavonoids and tocotrienols.

The invention provides a method for extracting active ingredients from tangerine seeds. The method comprises the following steps of: crushing the tangerine seeds, performing supercritical extraction of tangerine oil, performing immersion and homogenizing treatment on oil-removed tangerine seed residue, performing centrifugal separation on the homogenate, performing ultra-filtration treatment on supernate A, and performing affinity chromatography on filtrate and the like to finally obtain limonin with high purity and other active ingredients such as acidic limonin aglycon, nomilin, neutral limonin aglycon (containing no limonin or nomilin) and the like extracted from the waste tangerine seeds so as to realize comprehensive utilization of a tangerine seed resource. The method has high yield, the consumption of an organic solvent is greatly reduced in the extraction process, the flammable and combustible problems caused by extraction with the organic solvent are avoided, the production cost is reduced, and the environmental pollution is reduced.

The invention relates to the field of pharmaceutical chemistry and particularly relates to water-soluble limonin-7-amino derivatives (I) and (II) shown in the specification, and a preparation method and medicine application thereof. Pharmacological experiments prove that the compounds disclosed by the invention have the analgesic and anti-inflammatory effects and can be clinically used for relieving pain and inflammatory diseases.

The invention discloses a novel limonin compound as well as a preparation method and a medical application thereof. The compound which is reported for the first time is a limonin compound having a novel structure and can be obtained by extracting, separating and purifying from dried roots of attalanteae buxifoliae. In-vitro experiments prove that the compound has the capabilities of reducing ischemia reperfusion injury of human hepatocytes and playing a role of hepatic cell protection and can be used for being developed into medicaments for protecting the liver.

The invention provides an anti-hepatitis C composition including: an effective amount of limonoid compound, wherein the structure of the limonoid compound is shown as Structure (I):where R1 comprises H or OAc and R2 comprises H or COCH(CH3)2; and a pharmaceutically acceptable carrier or salt, and the anti-hepatitis C composition is used for inhibiting hepatitis C virus or treating hepatitis C. The invention also provides a method for treating hepatitis C and a method for preparing a drug for inhibiting hepatitis C viruses or treating hepatitis C.

Methods are disclosed for fortifying a sports drink with one or more citrus phytochemicals while concealing the bitter taste of these compounds in the beverage. These methods comprise microencapsulating the citrus phytochemicals and adding the microencapsulated citrus phytochemicals to the beverage. Also disclosed are sports drinks fortified with one or more microencapsulated citrus phytochemicals but which do not have the bitter taste characteristics of these compounds.

The invention discloses a preparation method of limonin extracts and application of the limonin extracts to preparation of diabetes treatment medicine. The limonin extracts are prepared by the following method that szechwan chinaberry leaves and folium toonae sinensis are used as raw materials to be extracted, separated and purified to obtain the limonin extracts consisting of aphanamixoid C, aphanamixoid C and chukvelutilide K. The limonin extracts provided by the invention have the important significance on the preparation of diabetes treatment medicine.

The invention discloses a special compound high-quality feed additive for freshwater shrimps. The special compound high-quality feed additive for the freshwater shrimps is prepared from the following substances in parts by weight: 4-6 parts of L-carnitine tartrat, 5-8 parts of saccharicterpenin, 6-8 parts of limonin, 4-7 parts of a compound vitamin additive, 2-3 parts of beneficial bacteria, 10-15 parts of a Chinese herbal medicine additive, 8-10 parts of medical stones and 2-5 parts of trimethylamine oxide. Aiming at physiological properties and growth rules of the freshwater shrimps, the different components are selected to be combined to obtain the feed additive, so that the growth of the freshwater shrimps can be accelerated, and the immunological competence and anti-stress capability of organisms also can be improved; furthermore, the special compound high-quality feed additive also has the effects of adsorbing harmful substances, resisting diseases and sterilizing and purifying the water quality, and the growth environment of the freshwater shrimps is optimized; the product has harmless components and does not cause medicine residues and environment pollution; the yield and quality of the freshwater shrimps are commonly improved; and a production method is simple and the components are easy to obtain, so that the special compound high-quality feed additive has a very good popularization value.

The invention relates to a method for extracting limonin and nomilin. The method comprises the following steps: degreasing a raw material, and then subjecting the degreased raw material to enzymatic hydrolysis with a wheat koji compound enzyme solution; removing supernatant liquid to obtain an enzymatic hydrolysis base material; adding a sodium salicylate solution for extraction, and separating aliquid extract; adjusting the pH value of the extract to 4.6-5.0, loading a supernatant onto macroporous resin, carrying out elution by using an ethanol solution with a concentration of 20-25% so as to remove impurities, then carrying out elution by using an ethanol solution with a concentration of 85-90%, collecting eluates, recovering the ethanol, and carrying out drying to obtain an extract A;dissolving the obtained extract A with a mixed solution of dichloromethane and isopropyl alcohol by heating, carrying out filtering to remove insoluble matters, and then successively carrying out cooling and crystallized so as to obtain limonin crystals; and subjecting mother liquid to pressure-reduced evaporation to dryness so as to obtain an extract B, dissolving the obtained extract B with a mixed solution of ethyl acetate and ethanol by heating, carrying out filtering to remove insoluble matters, and then successively carrying out cooling and crystallized so as to obtain nomilin crystals.The extraction method of the invention is low in cost and high in yield and product purity, and reduces environmental pollution.

The invention relates to the field of pharmaceutical chemistry and in particular relates to a type of water-soluble limonin derivatives (I) and (II) and preparation methods thereof. Pharmacodynamic experiment results prove that the compound disclosed by the invention has the effects of treating inflammations, rheumatoid arthritis, pain and the like. The structural formula is as shown in the specification.

The invention discloses a method for preparing a total limonin extract of a cordia dichotoma fruit. The total limonin extract of the cordia dichotoma fruit is prepared according to the following method comprising the steps: extracting the cordia dichotoma fruit, serving as a raw material, at low temperature by using 75 to 100 percent of ethyl alcohol, and purifying the cordia dichotoma fruit through MCI gel column chromatography and the like to obtain the total limonin extract of the cordia dichotoma fruit.

The invention relates to an application of limonoids in preparation of medicine for treating autoimmune diseases, wherein the limonoids include a structure represented as the formula (I), wherein R isoptionally selected from hydroxylated alkyl groups. The invention further relates to an application of the limonoids in preparation of a medicine for inhibiting differentiation of T-helper cell 17, and an application of the limonoids in preparation of a medicine for inhibiting differentiation of T-helper cell 1, T-helper cel 2 or regulative T cells. A method of screening substances capable of inhibiting cells of one or more of Th17, Th1, Th2 and Treg, which can be used for treating the autoimmune diseases. The limonoids can inhibit the differentiation of the Th17, Th1, Th2 and / or Treg cells.

The invention discloses a method for preparing obakulactone from an ethanol waste liquid of separating berberine from phellodendron bark, which comprises the following steps: by using a filtrate which is obtained by separating a crude product berberine from a phellodendron bark ethanol extracting solution as a raw material, carrying out enzymolysis with composite enzymes to obtain a precipitate, carrying out reflux extraction with ethanol, filtering to obtain a filtrate, and concentrating the filtrate under reduced pressure to recover ethanol to obtain a concentrated solution; and enriching the concentrated solution by a macroporous adsorbent resin process, eluting to obtain an eluate, concentrating the eluate, crystallizing and drying to obtain the obakulactone product. The yield of the obtained obakulactone is up to 0.35% (relative to the phellodendron raw material) or so, and the content is up to 80% above. The method can comprehensively utilize the phellodendron resources, changes wastes into valuable substances, reduces the environmental pollution, has the advantages of simple separation technique, high recovery rate and high limonin content, does not use any toxic organic solvent, is environment-friendly and is suitable for industrial production.

The invention discloses a limonin compound as well as a preparation method and a neuroprotective effect thereof, belongs to the field of drugs and in particular relates to a new limonin compound separated from dried whole herb of ruta graveolens as well as a preparation method and a medical application thereof. The compound is reported for the first time, can be obtained by extracting from the dried whole herb of ruta graveolens, separating and purifying and is high in purity. In vitro tests prove that Abeta1-40 toxicity can damage nerve cells and cause large-scale PC12 apoptosis, cell viability is reduced, and apoptosis situation is alleviated after intervention of a compound (I), so that the limonin compound can be further researched and developed into a neuroprotective drug.

The invention discloses a novel limonin compound as well as a preparation method and medical application thereof. The compound is reported for the first time, is a limonin compound with a novel structure, and can be extracted, separated and purified from the dried whole herb of Grace. In vitro tests prove that the compound can inhibit the proliferation of gastric carcinoma cells, and promote the apoptosis of the cells, and the growth inhibiting effect on the gastric carcinoma cells is strengthened gradually along with the increase of concentration, these findings provide a basis for a clinical research of drug of the compound (I) for curing gastric carcinoma, and the compound can be developed into a drug for curing the gastric carcinoma.

The invention discloses a novel limonoid as well as a preparation method and medical application thereof, and belongs to the technical field of medicines. The novel limonoid is reported for the first time, is novel in structure and can be obtained by extraction, separation and purification of dry fructus aurantii immaturus. In-vitro tests show that the novel limonoid has a neuroprotection function on A beta(25-35)-induced hippocampal neuron injuries, also has the function of relieving A beta(25-35)-induced hippocampal neuron toxic injuries and can be used for developing neuroprotection medicines.

The invention provides an anti-hepatitis C composition which comprises therapeutically effective dose of limonoid compound or pharmaceutical salts thereof, the molecular formula of the limonoid compound is shown as formula (I), wherein R1 is H or OAc, and R2 is H or COCH(CH3)2; the composition further comprises a pharmaceutical carrier; and the anti-hepatitis C composition is used for inhibiting hepatitis C virus or treating hepatitis C. The invention further comprises a method for preparing drugs for inhibiting the hepatitis C virus or treating the hepatitis C. (Formula I).

The invention relates to a combination product comprising limonoids (pharmacologically acceptable derivatives, esters, steric isomers, salt or prodrugs) and sulfonylurea (such as glibenclamide, gliclazide, glipizide, gliquidone and glimepiride). The invention further relates to an application of the combination product to treatment and / or prevention of diabetes-related diseases and the like.

Methods and compositions for activating PLTP gene expression include administering an effective amount of a limonoid.

The present invention discloses a new limonin compound, a preparation method and medical uses thereof. According to the present invention, the compound is firstly reported, is the limonin compound having a novel structure, and can be obtained by extracting the dried root of Atalantia buxifolia(Poir.)Oliv and carrying out separation purification; the in vitro test results prove that the compound provides protection effects for H2O2 oxidative injury ECV-304 cells; and the determination results of the MDA content, the SOD activity and the GSH-Px activity of the cell supernatant prove that the compound (I) has free radical and reactive oxygen scavenging anti-oxidation property, and can be used for developing blood vessel protection drugs.

The invention relates to a cortex dictamni antiviral active part and a preparation method, content determination method and application thereof. The cortex dictamni antiviral active part with contentof limonoid compound of more than 50% is obtained by using the preparation method, the preparation process is simple, the operation is easy, and the cost is low. Experiments show that the cortex dictamni effective part obtained by the preparation method has very good antiviral effects, and then the fact that the higher the total content of the two compounds of obakunone and fraxinellone is, the stronger the antiviral effect is is further found through experiments. The invention further discloses the content determination method and main component range of the cortex dictamni antiviral active part, so that the promotion and control of quality standards are facilitated in the later development and application.

The invention discloses a method for preparing a fermented vinegar beverage from navel orange peel residues and is characterized in that the method comprises the following steps: performing acetic acid fermentation to orange peel residue wine which serves as raw material, sterilizing, storing, blending and packaging. The invention has the advantages that: the method utilizes the navel orange peel residues which account for 40% to 60% of navel oranges from south Jiangxi province and rich in pectins, essential oils, flavonoid compounds, hesperidin, limonin, vitamins and soluble dietary fibers, achieves the purposes of fully utilizing navel oranges from south Jiangxi province and turning waste into treasure, conforms to the low-carbon and environment-friendly requirements, increases the added value of navel orange products, and is significant in development of navel orange industry.