Method for extracting active ingredients from tangerine seeds

Abstract

The invention provides a method for extracting active ingredients from tangerine seeds. The method comprises the following steps of: crushing the tangerine seeds, performing supercritical extraction of tangerine oil, performing immersion and homogenizing treatment on oil-removed tangerine seed residue, performing centrifugal separation on the homogenate, performing ultra-filtration treatment on supernate A, and performing affinity chromatography on filtrate and the like to finally obtain limonin with high purity and other active ingredients such as acidic limonin aglycon, nomilin, neutral limonin aglycon (containing no limonin or nomilin) and the like extracted from the waste tangerine seeds so as to realize comprehensive utilization of a tangerine seed resource. The method has high yield, the consumption of an organic solvent is greatly reduced in the extraction process, the flammable and combustible problems caused by extraction with the organic solvent are avoided, the production cost is reduced, and the environmental pollution is reduced.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of natural product active ingredients, and in particular relates to a method for extracting active ingredients from orange pits. Background technique [0002] Limonin analogues are a class of biologically active ingredients in orange pits, mainly including limonin acid aglycone, limonin, nomilin, limonin neutral aglycone (excluding limonin and nomilin) , all belong to triterpenoids, and have significant effects in promoting cancer cell apoptosis, regulating low-density lipoprotein, treating colon cancer, antibacterial and insecticidal, etc. Therefore, the application and development of limonin analogues has great medical value and Broad market prospects. [0003] At present, the extraction and separation process of limonin and its analogues requires a large amount of organic solvents and low yield. In addition to cost factors, my country is still unable to realize the large-scal...

AI Technical Summary

Problems solved by technology

[0004] There are three main problems in the technology of extracting active ingredients from orange stones: first, the extracted limonin has a low yield or low purity, and the extraction and purification process needs to be perfected; secondly, a large amount of Organic solvents such as sherwood oil, acetone, ethyl acetate and ethanol are used as degreasing or extraction solvents, and these organic solvents pollute the environment, and are inflammable and explosive, and are difficult to preserve; again, the current method only extracts the single component of orange stone Separation, failing to organically combine the extraction and separation of various active ingredients in the orange core, thus increasing the production cost

For example, the patent with the publication number CN101367823A: The method for separating limonin, evodiamine and evodiamine from Evodia rutaecarpa: It is recorded that limonin with a purity of 99% is obtained by organic solvent extraction, column chromatography, and efficient liquid phase separation. , the problem of this extraction process: the yield is low, only 0.1%, and the cost is too high, it is only suitable for experimental preparation, and cannot realize industrialization

For example, the patent with publication number CN101781355A: preparation method and composition of limonin and its application, the yield of limonin obtained by this process is 1.68%, and the purity is 98.5%. It seems that the yield and purity are good, but the extraction method It is obviously contrary to the objective facts, because limonin is a low-to-medium polar molecule. Under acidic conditions, limonin is closed-loop and insoluble in water and glacial acetic acid. It is impossible to extract lemons with glacial acetic acid. Limonin, through the dissolution experiment, it can be proved that limonin is insoluble in acetic acid

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