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Detection method for limonins components in szechwan Chinaberry fruit extractive

A technology of limonoids and detection method, which is applied in the field of detection of limonoids in Toosendan extract, can solve problems such as not seen, and achieve the effects of strong specificity, easy implementation, and scientific methods

Active Publication Date: 2013-11-20
SHANGHAI GREEN VALLEY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research on the analysis of the existence form and content of Nepali components in the fruit extracts of neem plants.

Method used

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  • Detection method for limonins components in szechwan Chinaberry fruit extractive
  • Detection method for limonins components in szechwan Chinaberry fruit extractive
  • Detection method for limonins components in szechwan Chinaberry fruit extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 toosendan extract

[0021] Take 4 kg of Toosendan medicinal material, crush it into coarse powder, reflux extract with 50 L of 75% ethanol for 3 times, each time for 2 hours, combine the extracts, recover the solvent under reduced pressure, and obtain 550 g of Toosendan extract, disperse it with 4 L of water, and disperse it with acetic acid The ethyl acetate was shaken and extracted 4 times, 4 L each time, the ethyl acetate extracts were combined, and the solvent was recovered under reduced pressure to obtain Toosendan extract (180 g).

Embodiment 2

[0022] The HPLC / ELSD detection method of limonoids component in the Toosendan extract of embodiment 2

[0023] (1) Preparation of reference substance solution: take 1-deacetyl-niporinin B (reference substance 1: 0.7746mg / ml), niporinin A (reference substance 2), and niporinin B (reference substance 3), toosendanin (reference substance 4) and toosendanin (reference substance 5; 0.3445mg / ml) appropriate amount, accurately weighed, and prepared with methanol, to obtain.

[0024] (2) Preparation of the test solution: take an appropriate amount of Toosendan medicinal material sample or reference medicinal material, grind it finely (through a No. 3 sieve), take about 5.0g, accurately weigh it, place it in a 100ml measuring bottle, and accurately add ethyl acetate 50ml of ester, plugged tightly, weighed, extracted under reflux for 3 hours, placed at room temperature, weighed, made up for lost weight with ethyl acetate, shaken well, filtered, took the subsequent filtrate, accurately m...

Embodiment 3

[0033] TLC identification of limonoids in Example 3 Toosendan extract

[0034] Toosendan medicinal material (MT-1), 5 batches of commercially available Toosendan medicinal herbal pieces (MTp-1~MTp-5) and Toosendan extract (MT-2) were taken respectively, and Toosendan as a control medicinal material (MT-R) and 1-deacetyl nipoorinine B reference substance (1) carry out TLC identification jointly (see attached figure 2 ). Reference substance solution, need testing solution are with embodiment 2.

[0035] Thin-layer chromatography identification: test according to thin-layer chromatography (Appendix VIB of Chinese Pharmacopoeia 2010 Edition), draw each 10-20 μ l of each reference substance solution and sample solution, respectively spot on the same silica gel G thin-layer plate, and use petroleum ether ( 60~90°C)-ethyl acetate (1:1) as the developer, develop, take out, dry in the air, spray with 10% sulfuric acid ethanol solution, and heat at 105°C until the chromatographic spo...

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Abstract

The invention relates to a detection method for limonins components in a szechwan Chinaberry fruit medicinal material and an extractive of the szechwan Chinaberry fruit medicinal material. The detection method comprises the following steps of: carrying out quantitative analysis on 1-deacetyl nimbolinin B and carrying out qualitative and quantitative analysis on one or more components of nimbolinin A, nimbolinin B, melia toosendan linin and toosendanin. The detection method particularly comprises the following steps of: adopting the 1-deacetyl nimbolinin B and other component comparison products of the type and adopting a high performance liquid-evaporative light-scattering detector to carry out the quantitative analysis on the 1-deacetyl nimbolinin B and the qualitative and quantitative analysis on the other components; carrying out thin-layer identification; adopting a color developing method to determine the content of total triterpenes. According to the detection method provided by the invention, the 1-deacetyl nimbolinin B and the components of the same type are used as the comparison products to systematically establish a nimbolinins component qualitative and quantitative analysis method for the first time; the method is scientific and reasonable, and is simple and feasible; the detection method is suitable for the szechwan Chinaberry fruit medicinal material and a processed product and the quality control of the nimbolinin type limonins components in congener fruit extractives.

Description

technical field [0001] The invention relates to a method for detecting limonoids in Toosendan extract, and also relates to the application of the detection method in quality control of Toosendan and fruit extracts of the same genus. technical background [0002] Toosendan is the mature fruit of Melia toosendan Sied.Et Zucc., a plant of Meliaceae. It is a traditional Chinese medicine. Abdominal pain and other syndromes have a history of nearly 2,000 years of medication, which are recorded in Chinese pharmacopoeias. Toosendan is slightly poisonous and needs to be processed before use; its processing methods include frying, steaming, blasting, calcining, burning, etc., and the mainstream variety is stir-frying. Modern research results show that Toosendan extract has various pharmacological activities such as antibacterial, antiviral, anti-inflammatory, analgesic, and insecticidal. Limonoids are the main pharmacologically active ingredients in Melia plants, which are complex t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90G01N30/36
Inventor 刘江云蔡金娜沈丽琴郝丽莉张燕苏胜杨世林
Owner SHANGHAI GREEN VALLEY PHARMA
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