Method for cultivating ganoderma lucidum karst

A cultivation method and technology of Ganoderma lucidum, applied in the field of artificial cultivation of medicinal fungi, can solve the problems of long production cycle of fruit body cultivation, low content of biologically active substances, obstacles to mass production of Ganoderma lucidum, etc. The effect of bacterial contamination rate

Active Publication Date: 2017-03-08
北京京诚生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The fruiting bodies (or sclerotia) produced by traditional wild collection or artificial cultivation are scarce in wild resources, the fruiting body cultivation and production cycle is long (4-5 months), production is controlled by climate and other conditions, and the content of biologically active substances is low and many other factors Restrictions have hindered the mass production of Ganoderma lucidum

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  • Method for cultivating ganoderma lucidum karst
  • Method for cultivating ganoderma lucidum karst

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A cultivation method of Ganoderma lucidum, comprising the following steps:

[0046] 1) Inoculate Ganoderma lucidum (Leyss.: Fr.) Karst parent species into the parent species medium and culture at a constant temperature of 22-26°C for 5-7 days to obtain the parent species of Ganoderma lucidum. The mother seed medium includes the following components: 250g / L potato, 25g / L glucose, 3g / L beef extract, 6g / L peptone, and 15-20g / L agar powder.

[0047] 2) Inoculate the parent ganoderma seed into the original seed medium and cultivate it at a temperature of 20-22° C. and a humidity of 55-65% to obtain the original ganoderma seed. The original seed medium includes the following components by weight: 70 parts of sawdust, 6 parts of corncobs, 15 parts of wheat bran, 2 parts of brown sugar, 1 part of gypsum powder, KNO 3 0.25 parts; the water content of the original seed culture medium is 55-60%; the original seed culture medium is made into a 500mL original seed bottle. The pre...

Embodiment 2

[0055] A cultivation method of Ganoderma lucidum, the only difference from Example 1 is that the mother seed culture medium includes the following components: 200g / L of potato, 20g / L of glucose, 3g / L of beef extract, 4g / L of peptone, 15g of agar powder / L; the original seed medium includes the following components by weight: 60 parts of sawdust, 5 parts of corncobs, 10 parts of wheat bran, 1 part of brown sugar, 1 part of gypsum powder, KNO 3 0.05 parts; the water content of the original seed medium is 55-60%; the cultivar medium includes the following raw materials in parts by weight: 50 parts of corn stalks, 30 parts of sawdust, 10 parts of pig manure, 10 parts of cow dung, rapeseed 5 parts of meal, 5 parts of humic acid, 1 part of Armillaria fermented liquid, 1 part of Bacillus subtilis fermented liquid, 1 part of gypsum; the effective number of viable bacteria in the Armillaria fermented liquid is 1.0-2.0×10 6 / mL, the number of effective viable bacteria in the Bacillus s...

Embodiment 3

[0058] A cultivation method of Ganoderma lucidum, the only difference from Example 1 is: the Ganoderma lucidum is Ganodermasinense J.D.Zhao, L.W.Hsu et X.Q.Zhang. The average daily growth rate of mother species, original species, cultivated species, and cultivation bag mycelium can reach 18-20mm / d. It generally takes 30-45 days from primordia to fruiting body maturity, and can be harvested 1.5-2 months after cultivation; based on the dry weight of 1000kg of dry substitute material, 90-115kg of Ganoderma lucidum fruiting bodies can be harvested in the same year.

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Abstract

The invention belongs to the technical field of artificial cultivation of medicinal fungi, and particularly relates to a method for cultivating ganoderma lucidum karst. The method includes the steps of obtaining ganoderma lucidum karst mother species, ganoderma lucidum karst protospecies and ganoderma lucidum karst cultispecies respectively, and conducting substitute cultivation of the ganoderma lucidum karst cultispecies to obtain ganoderma lucidum karst, wherein a cultispecies culture medium is prepared from corn straw, sawdust, pig manure, cow dung, rapeseed meal, humic acid, an amillariella mellea fermented liquid, a bacillus subtilis fermentation liquid and gypsum, and a culture medium of the substitute cultivation is same as the cultispecies culture medium. According to the method, the yield of ganoderma lucidum karst sporophore and conidial powder is significantly improved, and the contents of effective components, like ganoderma lucidum karst polysaccharide and ganoderma lucidum karst total triterpenoid, are significantly improved.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of medicinal fungi, and in particular relates to a cultivation method of ganoderma lucidum. Background technique [0002] Ganoderma lucidum (Ganoderma Lucidum Karst) is the fruiting body of the polyporaceae fungus Ganoderma lucidum. It has the effects of invigorating qi and calming the nerves, relieving cough and asthma, and prolonging life. For dizziness and insomnia, palpitation, shortness of breath, neurasthenia, fatigue and cough. Ganoderma lucidum is a kind of precious Chinese medicine in the treasure house of Chinese medicine, and it is an edible fungus integrating health care and medicine. "Shen Nong's Materia Medica", "Baopuzi", "Compendium of Materia Medica" and other ancient books all have records on its efficacy. Modern medical research has proved that active ingredients such as polysaccharides, triterpenoids, and sterols in Ganoderma lucidum have the functions of anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G3/00C05F17/00A01G1/04C12R1/645
CPCA01G18/00A01G18/20A01G18/40Y02W30/40
Inventor 马传贵张志秀
Owner 北京京诚生物科技有限公司
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