Method for extracting limonin analogues from tangerine seeds

A technology of limonin and analogs, applied in the direction of steroids, organic chemistry, etc., to achieve the effect of reducing environmental pollution, reducing waste, and good technical effects

Inactive Publication Date: 2015-02-04
SHAANXI TEEGOO BIOTECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a new approach for the development and utilization of orange stones, to reduce the

Method used

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  • Method for extracting limonin analogues from tangerine seeds
  • Method for extracting limonin analogues from tangerine seeds

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Embodiment Construction

[0025] The implementation of the present invention will be described in detail below in conjunction with the accompanying drawings and examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention. It should be noted that, as long as there is no conflict, each embodiment and each feature in each embodiment of the present invention can be combined with each other, and the formed technical solutions are all within the protection scope of the present invention.

[0026] Specifically, as figure 1 , 2 Shown, a kind of method for extracting limonoid analogue from orange core, comprises:

[0027] Step 1: Obtain the raw materials. The content of the raw materials is detected by ultrasonic extraction with 90% ethanol for 1 hour, and the extract is directly detected. The content of the raw materials requires limonin ≥ 0.6% and nomilin ≥ 0.3%;

[0028] Step 2: Carry out sh...

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Abstract

The invention discloses a method for extracting limonin analogues from tangerine seeds. The method comprises the following steps: firstly, obtaining raw materials, wherein the raw materials are subjected to ultrasonic extraction with 90% ethanol for 1 hour to obtain an extract, the extract is directly detected, the content of limonin is required to be equal to or greater than 0.6% and the content of nomilin is equal to or greater than 0.3%; secondly, breaking hulls of the raw materials and pulverizing until the hulls of the raw materials are completely broken; thirdly, extracting the raw materials with 90% ethanol for three times and 1.5 hours each time and ultimately controlling that the active ingredients in the residue account for no more than 3% of the raw material total target, wherein the ratio of material to solvent is 1 to 4 to 4 to 3; fourthly, concentrating the extract until the degree of alcohol is 20%, standing for 12 hours and removing floating oil on the surface of the liquid; fifthly, continuously recovering the solvent from a concentrated solution, controlling the extraction specific gravity to be 1.03+/-0.1, standing and crystallizing for 12 hours; sixthly, extracting an upper layer supernatant, filtering the lower layer precipitate with filter cloth and centrifuging; and seventhly, after centrifuging, carrying out coarse crystallization and vacuum drying at a temperature of less than 80 DEG C.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural medicines, and in particular relates to the use of mandarin orange pit as a raw material, extraction by methanol heat reflux, impurity-removing treatment of the extract, concentration of the extract with a specific specific gravity to precipitate coarse crystals, and degreasing and separation to obtain limonoid analogues The crude product can be obtained by recrystallization to obtain limonin essence. [0002] Background technique [0003] Limonoids (Limlclis) are a class of triterpenoids present in Rutaceae and Meliaceae plants. In recent years, studies have shown that limonoids can strongly activate the activity of the main detoxification enzyme in the body - glutathione-transferase (GSH-transferase). A large number of animal experiments have shown that limonoids have the ability to inhibit the Liver cancer, small intestine cancer, oral cancer and other cancers are a class of ph...

Claims

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Application Information

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IPC IPC(8): C07J73/00
CPCC07J73/008
Inventor 张必荣李秀妮张敏
Owner SHAANXI TEEGOO BIOTECH CO LTD
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