Extraction method of limonin

A technology of limonin and extraction method, which is applied in the extraction of plant natural drug components and in the field of biomedicine, can solve the problems of not being able to be directly used as medicine, lack of asphalt-based waterproof rubber, etc., to achieve reduced content, high product purity, well-designed effects

Active Publication Date: 2020-08-07
广州市莱檬生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adds chemical solvents such as acetone and chloroform as extracts, and the obtained product cannot be directly used as medicine, especially as food and medicine, which is directly taken internally by the human body, and needs further improvement
[0005] In summary, the prior art still lacks a bitumen-based waterproof adhesive with high extraction efficiency and good extraction method

Method used

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  • Extraction method of limonin
  • Extraction method of limonin

Examples

Experimental program
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Embodiment 1

[0030] A method for extracting limonin, comprising the following steps:

[0031] (1) Pretreatment, soak 10kg of lemon seeds and lemon peel residue in 200kg of water, add 10g of Celluclast 1.5L cellulase, 5g of subtilisin, and 5g of 1,4-α-D-glucanohydrolase to decompose impurities;

[0032] (2) Extraction, add 2g of Novozymes Celluclast 1.5L cellulase to the pretreated product, treat for 1 hour, then add nitric acid for 1 hour, and the pH of the reaction system during acidolysis is 3;

[0033] (3) solid-liquid separation, using a plate and frame filter to remove solid residues, leaving liquid to obtain the initial product;

[0034] (4) Remove pectin, concentrate the initial product in vacuum, measure the sugar content of the concentrated solution by a hand-held refractometer sugar meter, stop the vacuum concentration when the sugar content reaches 8%, and then use 98% ethanol, 94% isopropanol mixed solution to carry out alcohol precipitation, The volume ratio of ethanol to iso...

Embodiment 2

[0037] The difference between this embodiment and embodiment 1 is that the extraction process is not used.

[0038] A method for extracting limonin, comprising the following steps:

[0039] (1) Pretreatment, soak 10kg of lemon seeds and lemon peel residue in 200kg of water, add 10g of Celluclast 1.5L cellulase, 5g of subtilisin, and 5g of 1,4-α-D-glucanohydrolase to decompose impurities;

[0040] (2) Extraction, add 1g of Novozymes Celluclast 1.5L cellulase to the pretreated product, treat for 2 hours, then add nitric acid for 2 hours, and the pH of the reaction system during acidolysis is 4;

[0041] (3) solid-liquid separation, using a plate and frame filter to remove solid residues, leaving liquid to obtain the initial product;

[0042] (4) Remove pectin, concentrate the initial product in vacuum, measure the sugar content of the concentrated solution by a hand-held refractometer sugar meter, stop the vacuum concentration when the sugar content reaches 8%, and then use 98% e...

Embodiment 3

[0045] The main difference between this embodiment and Example 1 is that the initial product will be left to stand for 7 days in step (3).

[0046] A method for extracting limonin, comprising the following steps:

[0047] (1) Pretreatment, soak 10kg of lemon seeds and lemon peel residue in 200kg of water, add 10g of Celluclast 1.5L cellulase, 5g of subtilisin, and 5g of 1,4-α-D-glucanohydrolase to decompose impurities;

[0048] (2) Extraction, add 2g of Novozymes Celluclast 1.5L cellulase to the pretreated product, treat for 1 hour, then add nitric acid for 1 hour, and the pH of the reaction system during acidolysis is 3;

[0049] (3) Solid-liquid separation, using a plate and frame filter to remove solid residues, leaving liquid to obtain the primary product, which will be left to stand for 7 days;

[0050] (4) Remove pectin, concentrate the initial product in vacuum, measure the sugar content of the concentrated solution by a hand-held refractometer sugar meter, stop the va...

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Abstract

The invention discloses an extraction method of limonin. The extraction method comprises the following steps: pretreatment: adding lemon seeds and lemon peel residues into water, carrying out soaking,and adding a pretreatment enzyme to decompose impurities; extraction: adding an extracting enzyme into a pretreatment product for enzymolysis, adding acid for acidolysis, performing solid-liquid separation, removing solid residues, and reserving liquid to obtain a primary product; removal of pectin: carrying out vacuum concentration on the primary product, adding alcohol for alcohol precipitation, separating a floccule precipitate, and reserving liquid; and purification: carrying out extracting at first, and then conducting separating by using macroporous resin. The extraction method disclosed by the invention is reasonable in design, all the steps are associated with one another, impurities are removed one by one, and extraction and macroporous resin separation are combined for use; andextraction is carried out at first and then macroporous resin separation is carried out, and the advantages of two separation means are combined, so separation efficiency is high, and product purity is high.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, relates to the technical field of extraction of plant natural medicinal components, in particular to a limonin extraction method and application thereof. Background technique [0002] Limonin, also known as Phellodendron lactone, Evodicarlide, has various biological activities such as anti-tumor, insect antifeedant, anti-virus, analgesic, anti-inflammatory, hypnotic, etc. It is an important natural pharmaceutical raw material and can be used for the treatment of Pneumonia, functional food additives, anti-cancer food, pesticides, feed additives, etc. At present, the extraction and separation of limonin adopts the methods of multiple extractions, multiple column chromatography and preparation of liquid phases. This extraction method is relatively cumbersome and the extraction rate is low. [0003] CN102442916A discloses a method for separation and purification of limonoid compound C in Tianguo...

Claims

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Application Information

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IPC IPC(8): C07J73/00A61P1/16A61P11/00A23L33/105
CPCA23V2002/00A23L33/105A61P1/16A61P11/00C07J73/008A23V2200/30A23V2250/21
Inventor 廖劲松
Owner 广州市莱檬生物科技有限公司
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