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Test paper strip for testing bovine leukemia antibody with colloidal gold immune chromatography and preparation method thereof

An immunochromatography and bovine leukemia technology, which is applied in the field of animal quarantine and detection to achieve the effects of saving investment, increasing herdsmen's income and reducing feeding costs

Inactive Publication Date: 2008-11-12
中华人民共和国徐州出入境检验检疫局
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AI Technical Summary

Problems solved by technology

Determine whether there is bovine leukemia antibody in the serum according to whether there is a color band in the detection line and the quality control line

Method used

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  • Test paper strip for testing bovine leukemia antibody with colloidal gold immune chromatography and preparation method thereof
  • Test paper strip for testing bovine leukemia antibody with colloidal gold immune chromatography and preparation method thereof

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Embodiment Construction

[0018] As shown in the figure: a test strip for detecting bovine leukemia antibody by colloidal gold immunochromatography and its preparation method, including in vitro expression, separation and purification of bovine leukemia virus envelope glycoprotein gp51, and detection of bovine leukemia antibody by colloidal gold immunochromatography The test strip for leukemia antibody is characterized in that: construct recombinant prokaryotic expression vector pET-32a-gp51, induce expression of GP51 recombinant protein in Escherichia coli, purify His-gp51 fusion protein by chromatography, and fuse purified His-gp51 The protein and goat anti-bovine IgG were spotted on the nitrocellulose membrane as the detection line (T line) for capturing BLV antibody and the quality control line (C line) for capturing bovine IgG respectively. Colloidal gold-labeled goat anti-bovine IgG (Fc ) antibody was adsorbed on glass fiber to make a gold standard pad.

[0019] The test strip for detecting bovin...

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Abstract

The invention relates to a test strip for testing bovine leukemia antibody by using in vitro expression, separation and purification of cyst membrane glycoprotein gp51 of bovine leukemia virus (BLV), and gold immunochromatography and a preparation method thereof. Prokaryotic expression vector pET-32a-gp51 is constructed and recombined and the expression of GP51 recombined protein is inducted in colibacillus, chromatography purifies the recombined protein to obtain His-gp51 fusion protein, the purified His-gp51 fusion protein and goat-anti-bovine IgG are spotted on a nitrocellulose membrane for respectively being taken as testing line (T line) for capturing BLV antibody and quality control line (C line) for capturing bovine IgG, the goat-anti-bovine IgG (Fc) marked by the colloidal gold isabsorbed to a glassfiber to make into gold pad, so as to assemble the colloidal gold testing strip. Whether the BLV antibody exists in blood serum is determined by whether color belts appear on the testing line and the quality control line.

Description

technical field [0001] The invention relates to a test strip for bovine leukemia virus (BLV) envelope glycoprotein gp51 expression in vitro, separation and purification, and colloidal gold immunochromatography for detecting bovine leukemia antibody and a preparation method thereof, which belongs to animal quarantine detection technology and mainly applies in the field of public health. Background technique [0002] BLV detection methods mainly include virus direct detection, serological methods, molecular biological methods and other methods. Currently available serological methods mainly include agar diffusion assay (AGID), indirect immunofluorescence assay (IIFA), complement fixation assay (CFA), radiative immunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA). However, the detection period of the agar diffusion test is slightly longer, and it generally takes one to two days, while the detection speed of the ELISA method is relatively fast (it takes several hours...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531G01N33/52
Inventor 宋阳威张鑫宇张常印孙怀昌吴亚力何玲张敬友
Owner 中华人民共和国徐州出入境检验检疫局
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