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Double-antibody sandwich ELISA kit for detecting gosling plague antigen and application thereof

A double-antibody sandwich and kit technology, applied in the field of biotechnology detection, can solve problems such as diagnostic loss, and achieve the effects of simple method, easy operation and stable activity

Active Publication Date: 2015-08-12
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, although there are many detection methods, such as agar diffusion test and agar diffusion inhibition test, neutralization test and animal protection test, direct immunofluorescence diagnosis method, immunoenzyme dot method (Dot-ELISA), but there is a lack of corresponding methods in some grassroots units. The experimental equipment cannot be diagnosed in the first time, resulting in great losses

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  • Double-antibody sandwich ELISA kit for detecting gosling plague antigen and application thereof
  • Double-antibody sandwich ELISA kit for detecting gosling plague antigen and application thereof
  • Double-antibody sandwich ELISA kit for detecting gosling plague antigen and application thereof

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Embodiment Construction

[0035] The following examples further illustrate the content of the present invention, but should not be understood as restrictions on the present invention.Without departure from the spirit and essence of the invention, the modification or replacement of the methods, steps or conditions of the present invention belongs to the scope of the present invention.

[0036] If it is not specifically specified, the technical means used in the embodiments are the conventional means familiar to the technical personnel of the art.

[0037] 1. Establishing test process of dual anti -sandwiches ELISA antigen detection method

[0038] (1) Preparation of single cloning Hangzhou body

[0039] 1. Preparation of monoclonal antibodies

[0040] Use the super-speed centrifugal method to purify the Goose Parvovirus (GPV). After verification by electron microscope and SDS-PAGE, it is determined that GPVs with better purification effect have been obtained.

[0041] Use the above GPV as an immunogenic, im...

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Abstract

The invention provides a double-antibody sandwich ELISA kit for detecting a gosling plague antigen and an application thereof and belongs to the field of bio-technical detection. The kit includes: an ELISA plate coated by an anti-gosling plague virus (anti-GPV) monoclonal antibody, a GPV standard positive antigen, and enzyme-labeled anti-GPV monoclonal antibody aiming to different epitopes of the GPV. The kit is provided according to the following technical principle: ELISA is a technology that a known antigen or antibody is adsorbed onto the surface of a solid-phase carrier so that an enzyme-labeled antigen-antibody reaction is carried out on the solid-phase surface. The kit can be used for test large-molecular antigens or specific antibodies or the like, is quick, sensitive and simple and is easy to standardize.

Description

Technical field [0001] The present invention is the field of biotechnology testing, which involves the detection of small viruses for goose. Background technique [0002] Goose Parvovirus (GPV) is a small goose plague virus. The goose fine virus infection is a high degree of contact, acute, and sewage infectious disease caused by the small virus of the goose.duck.Strong infectious and high mortality.The chicks and ducks are characterized by myocarditis, pseudo -membrane enteritis, and acute hematic lesions of the whole body.At present, the disease is popular every year, and it is still one of the most important infectious diseases in the country where the goose industry and intensive breeding duck are caused, causing serious losses to the aquaculture industry.In 1956, Chinese scholars Fang Dingyi first discovered the disease in Yangzhou, Jiangsu, and separated the world's first GPV with goose embryo. [0003] At present, there are many detection methods, such as agar diffusion te...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 秦爱建钱琨石亚运郭卉宋娜邵红霞叶建强
Owner YANGZHOU UNIV
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