115 results about "Human albumin" patented technology

The invention relates to the field of protein cosmetics, in particular to human albumin anti-aging essence and a preparation method thereof. The human albumin anti-aging essence is prepared from the following components in parts by mass: 20 to 45 parts of human albumin; 5-15 parts of a red ginseng extract; 2.5-10 parts of a vitis amurensis seed extract; 1.5 to 5 parts of a blueberry peel extract; 20 to 40 parts of a humectant; 0.2 to 1.6 parts of a thickening agent; 0.01 to 0.1 part of an excipient; and the balance of deionized water. The human albumin anti-aging essence can be used for skin aging resistance, has the effects of resisting free radicals, increasing skin elasticity and the like, has good biocompatibility and effectiveness, and has important significance on skin aging resistance and skin improvement.

The invention provides a method for preparing recombinant human albumin with low O-glycosylation level, comprising taking at least one measure to improve the growth rate of the strain and reduce the O-glycosylation level of the protein during the fermentation process, and specifically comprising S1, seed liquid preparation; S2, BSM culture medium is put into a fermentation tank, the pH is adjusted to 5.0-5.8, sterilization is carried out, dissolved oxygen is corrected after cooling, temperature and ventilation volume are set, sterilized PTM1 solution is added to the culture medium, and the pH of the culture medium is adjusted to 5.0-5.8 again; S3, shaking flask seeds are connected to the fermentation tank, dissolved oxygen is corrected, and rotation speed is set; S4, when the dissolved oxygen increases suddenly, a glycerol feeding stage is entered, and glycerol feeding is added at a uniform speed; S5, glycerol feeding and methanol feeding are added simultaneously, pH is adjusted to 6.0-6.5, and the temperature is lowered; S6, methanol feeding is added; S7, after the fermentation is completed, the tank is released, the fermentation product is centrifuged, and the supernatant is collected; the method of the invention can reduce protein O-glycosylation, and the expressed recombinant protein contains a very low level of glycosylation modification.

The invention belongs to the field of immunological detection, and provides an anti-human albumin antibody 5H11 and application thereof. The anti-human albumin antibody provided by the invention can be specifically combined with human albumin and has relatively high stability. Based on the antibody, a kit capable of accurately, rapidly and massively detecting the content of HSA in a sample is developed. The double-antibody sandwich ELISA method for detecting the human albumin has high sensitivity, specificity and accuracy, can realize accurate and high-throughput detection of the content of albumin in human serum or other serum-like species containing the human albumin, and has a good application prospect in detection of the human albumin.

The invention discloses a method for cryopreserving mature dendritic cells, and the method for cryopreserving the mature dendritic cells comprises a dendritic cell cryopreservation solution. The dendritic cell cryopreservation liquid is mainly prepared from the following raw materials in percentage by volume: 15 to 16v / v percent of human albumin injection, 1 to 3v / v percent of antifreeze protein liquid, 17 to 20v / v percent of compound dextran 40 injection, 17 to 20v / v percent of compound electrolyte injection and 41 to 50v / v percent of CryoStorCS10, in addition, the cryopreservation liquid also comprises Setanaxib, and the concentration of the Setanaxib in the cryopreservation liquid is 20 [mu] mol / L. The cryopreservation liquid is used for cryopreservation treatment of the mature dendritic cells, through synergistic interaction among different components in the cryopreservation liquid, the recovery survival rate of the cryopreserved mature dendritic cells is larger than or equal to 80%, and the antigen presentation capacity of the mature dendritic cells can be preserved to a large extent.

The invention discloses a recombinant protein for detecting a para-tumor Yo antibody through a CBA method and application, and belongs to the technical field of biomedical engineering.The amino acid sequence of the recombinant protein sequentially comprises a secretory signal peptide, a CDR2 protein partial sequence, a CDR2L protein partial sequence, a transmembrane region and a fluorescent label, and the secretory signal peptide is human albumin signal peptide ALB; the transmembrane region is a CD8a hinge; and the fluorescent label is mCherry. A novel recombinant protein which can be stably over-expressed on a cell membrane of an eukaryotic cell is constructed by intercepting specific partial sequences of CDR2 protein and CDR2L protein and redesigning and fusing a fluorescent label by using a secretory signal peptide, a transmembrane sequence and a connecting peptide, and the recombinant protein retains respective core antigen regions of the CDR2 protein and the CDR2L protein, so that the specific partial sequences of the CDR2 protein and the CDR2L protein can be stably over-expressed on the cell membrane of the eukaryotic cell. The kit can effectively overcome the defects in the aspects of sensitivity and specificity, and when a CBA method is adopted for detection, the detection rate of the para-tumor Yo antibody can be remarkably increased, and the false positive rate is reduced, so that the requirements of clinical detection are better met.

The invention provides a recombinant engineering bacterium for preparing recombinant human albumin based on a transport secretion way and application, and relates to the technical field of gene engineering. One or more of endoplasmic reticulum related genes SRP54 and ERJ5, vesicle transport related genes YOS1 and SLY1 and Golgi apparatus related genes GOS1 and YIP1 are integrated into a recombinant engineering bacterium genome through a gene recombination technology, so that efficient secretory expression of recombinant human albumin is realized. According to the method provided by the invention, the expression quantity of the recombinant human albumin is remarkably improved, and the method has a wide application prospect.

The invention belongs to the field of immunological detection, and provides an anti-human albumin antibody 7G7, an antibody composition and application thereof. The anti-human albumin antibody provided by the invention can be specifically combined with human albumin and has relatively high stability. Based on the antibody, a kit capable of accurately, rapidly and massively detecting the content of HSA in a sample is developed. The double-antibody sandwich ELISA method for detecting the human albumin has high sensitivity, specificity and accuracy, can realize accurate and high-throughput detection of the content of albumin in human serum or other serum-like species containing the human albumin, and has a good application prospect in detection of the human albumin.

A majority of military casualties occur during the pre-hospital period. The cause of death is largely associated with massive traumatic bleeding that leads to organ damage due to sustained hypoxia. Currently, therapeutics are lacking at the point of injury to mitigate hypoxic damage and maintain the survivability of severe hemorrhage prior to reaching medical facilities. This invention addresses that by introducing a method of co-administering prolyl hydroxylase domain inhibitor (PHDi, MK-8617), anti-fibrinolytic agent (tranexamic acid), and bradykinin receptor antagonist (icatibant) as anti-hemorrhage agents in combination with resuscitation fluid treatment with colloid solution of 25% human albumin that has an advantage of relatively small volume requirement to maintain blood volume. In addition, a kit comprising anti-hemorrhage agents that can be easily carried to the battlefield is also provided here. The therapeutic application of those agents on or near the point of injury can stabilize hypoxia inducible factor-1 alpha and enhance blood clot formation, which improves the patient's cellular adaptation to hypoxia and reduce hemorrhage, and thus can decrease organ failure and increase the patient's survivability.

The invention relates to the technical field of protein cosmetics, in particular to human albumin antioxidant essence and a preparation method thereof. The human albumin antioxidant essence is prepared from the following components in parts by mass: 5 to 35 parts of human albumin; 10 to 25 parts of a ginseng extract; 0.2 to 0.8 part of a humectant; 0.01 to 0.1 part of a solubilizer; 0.2-1 part of fatty acid; 0.2 to 2 parts of amino acid; and the balance of deionized water. The human albumin anti-oxidation essence provided by the invention adopts the human albumin and the ginseng extract as active ingredients, has good biocompatibility and effectiveness, and has important significance on skin oxidation resistance and skin improvement.

The invention discloses a nano antibody 4C12 and application thereof in promoting purification of human albumin, and belongs to the technical field of polypeptides. The amino acid sequence of the nano antibody comprises an amino acid sequence as shown in SEQ ID NO. 1; and / or an amino acid sequence of a fusion protein with the same function, which is obtained by connecting tag protein to the N terminal and / or C terminal of the amino acid sequence as shown in SEQ ID NO.1. The nano antibody has extremely high affinity with the human albumin, the convective mass transfer advantages of the nano antibody 4C12 and affinity membrane chromatography are combined, and gel filtration is combined, so that efficient, rapid and large-scale preparation of the human albumin is realized.

The invention discloses a freeze-dried human rabies vaccine and a preparation method thereof, and belongs to the technical field of viral vaccines. The method comprises the following steps: (1) cell culture; (2) virus inoculation; (3) harvesting viruses; (4) concentration and purification: clarifying the harvested virus liquid through a filter, carrying out ultrafiltration concentration through an ultrafiltration membrane to obtain a concentrated solution, and purifying by adopting a column chromatography to obtain a chromatographic solution; (5) preparing a stock solution: inactivating and hydrolyzing the chromatographic solution to obtain a virus inactivation solution, and adding human serum albumin into the virus inactivation solution to obtain the stock solution; (6) preparing a semi-finished product: diluting with a stabilizer to prepare the semi-finished product, wherein the stabilizer comprises the following raw materials: human serum albumin, araboxylan, L-arginine hydrochloride, casein phosphopeptides, alpha-tocopherol nicotinate, a solvent and the like; and (7) preparing a finished product. The rabies vaccine disclosed by the invention is excellent in quality and stable in immune efficacy, and liquid is clear after redissolution.

The invention provides a recombinant pichia pastoris engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biochemistry and genetic engineering, the recombinant pichia pastoris engineering bacterium is a pichia pastoris strain in which an FDH1 gene as shown in SEQ ID NO.1 is subjected to targeted knockout, and a DAK2 gene as shown in SEQ ID NO.3, an SHM2 gene as shown in SEQ ID NO.5, an FBA1 gene as shown in SEQ ID NO.7 and an MTD1 gene as shown in SEQ ID NO.9 are inserted; the recombinant pichia pastoris engineering bacterium provided by the invention can obviously improve the yield of recombinant protein, and particularly, the yield of proteins such as recombinant human transferrin, recombinant human albumin, recombinant human basic fibroblast growth factors and the like is greatly improved.

The invention provides an albumin pichia pastoris feed additive as well as a preparation method and application thereof, and relates to the field of biological medicines. The pichia pastoris strain for expressing the recombinant human albumin is subjected to high-density fermentation culture and then is subjected to microwave-hot air combined drying to obtain the albumin pichia pastoris feed additive, and the feed additive is prepared from pichia pastoris produced by producing a thallus albumin product, so that the waste of resources is reduced, and the environmental pollution is effectively prevented; the method can be used in pig raising production, and provides a new feed research direction for animal breeding.

The invention relates to the field of protein, in particular to a preparation method of recombinant human albumin and a product thereof. The preparation method comprises the following steps: constructing recombinant engineering bacteria which can silence or weaken the expression of one or more genes of PEP4, YPS1, MKC7, PRC1 and PRB1 through a gene editing technology while expressing recombinant human albumin, and silence or weaken the expression of one or more genes of PMT1, PMT2 and PMT4 at the same time; and then, carrying out low O-glycosylation fermentation culture on the obtained engineering bacteria, and carrying out separation and purification to obtain a high-purity recombinant human albumin product. According to the present invention, the protein degradation and the O-glycosylation pathway are cooperatively regulated, and the specific fermentation process is combined, such that the yield of the obtained recombinant human albumin is significantly improved, the characteristics of low immunogenicity and low glycosylation level are provided, and the performance optimization and the clinical application expansion of the recombinant human albumin product can be significantly promoted.

The invention relates to the field of cosmetics, in particular to a recombinant albumin anti-aging composition as well as a preparation method and application thereof. The composition provided by the invention comprises the following components in parts by mass: 0.1-15.0 parts of recombinant human albumin; 0.1 to 10.0 parts of a grape wine vinasse polysaccharide extract; the content of the red raspberry extract is 0.1 to 5.0 parts. According to the composition disclosed by the invention, the recombinant human albumin, the wine vinasse polysaccharide extract and the red raspberry extract have a synergistic effect, so that the composition has the effects of inhibiting the activity of tyrosinase, promoting regeneration of collagen and elastin, tightening and resisting wrinkles and effectively resisting aging, and the composition disclosed by the invention is simple in component, good in biocompatibility and free of toxic and side effects. The sensitization of the wine vinasse extract and the red raspberry extract is improved, and the method can be widely applied to preparation of anti-aging products.

The invention provides industrially produced high-purity recombinant human albumin as well as a preparation method and application thereof. Only three-step chromatography is adopted, and high-yield and high-purity recombinant human albumin in gene editing pig plasma can be prepared. Chromatographic packing suitable for protein purification is screened through different purification small-scale experiments, and meanwhile, three types of chromatographic packing are finally confirmed by considering that the requirement for later-stage industrial amplification is met. By regulating and controlling conditions such as sample loading amount, sample loading concentration, pH of samples in different chromatography steps, conductivity value and the like, the recovery rate of albumin is reserved to the greatest extent, the purity of albumin is guaranteed by using three-time chromatography, and the method has the characteristics of high recovery rate, high purity and simplicity in operation, and can be suitable for large-scale preparation of high-purity plasma albumin.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.

A method of testing a test substance in an in vitro model of a human tracheobronchial respiratory tract, includes: providing the in vitro model including a cell culture including airway epithelial cells (AECs), a basolateral compartment below the AECs and an apical compartment above the AECs, wherein the AECs form a barrier between the basolateral AND apical compartments; adding the test substance to the apical and / or basolateral compartment; adding a tracer to the basolateral compartment, which is fluorescent, has a molecular weight within 5 kDa of human albumin and is added before, during or after adding the test substance; incubating the cell culture system in a presence of the tracer; collecting at least one sample from the apical compartment; and measuring a fluorescence thereof to determine an effect of the test substance on the AECs. A kit is also disclosed.

Abstract Methods for editing, e.g., introducing a heterologous transgene, within the human albumin gene (e.g., at intron 1) are provided. Abstract

Provided in the present invention is a cell freezing medium for clinical use. In particular, the cell freezing medium of the present invention comprises the following components: (1) human albumin; (2) cryoprotectant: the cryoprotectant comprises a combination of one or more of dimethyl sulfoxide, glycerol, and ethylene glycol; (3) a saline buffer; wherein the salt buffer is a solution containing Na+, K+, Mg+, Cl−, and CH3COO− ions; (4) a vitamin; and (5) an amino acid, wherein the human albumin concentration is 1%-20% (w / v). The cell, after long-term cryopreservation with the freezing medium of the present invention, has a high viability, and the cellular efficiency maintains a high uniformity. The grade of purity of the freezing medium of the present invention is the pharmaceutical grade or USP grade; and the freezing medium is safe and reliable for clinical use, and can be used or conventional adherent and suspension cells.

The present application relates to a recombinant engineering bacterium producing human albumin and its construction method and application, belonging to the field of genetic engineering technology. In the present application scheme, the human albumin gene sequence is codon-optimized to construct a highly efficient recombinant human albumin Pichia yeast strain, further overexpress PDI and SSB2, and the gene sequence of the overexpressed protein in the recombinant plasmid is codon-optimized, and after transformation into the recombinant human albumin Pichia yeast strain, a Pichia yeast genetic engineering strain with high expression of human albumin is successfully obtained, and the strain achieves efficient expression of human albumin through high-density fermentation, with a yield of up to 38.6 g / L, laying the foundation for the industrial application of recombinant human albumin, and having important industrial application prospects and economic value.

This application provides a method and apparatus for optimizing the codon sequence of human albumin, belonging to the field of data processing technology. Addressing the current problems of low synthesis efficiency and scarcity of human albumin drugs, it allows for the grouping of large datasets of human albumin amino acid sequences. An improved genetic optimization algorithm is then used to optimize the corresponding messenger RNA item by item, ultimately obtaining the optimal second target messenger RNA. When using this second target messenger RNA for protein expression to obtain the target recombinant human albumin, the expression efficiency of the host Pichia pastoris cells can be maximized, significantly improving the current synthesis efficiency of recombinant human albumin and greatly alleviating the problem of human albumin drug scarcity.

A method for preparing recombinant human albumin with high expression and low O-glycosylation level, comprising taking at least one measure during fermentation to improve the protein expression rate and reduce the O-glycosylation level of a protein; and specifically comprising: S1, seed solution preparation; S2, batch fermentation; S3, glycerol fed fermentation; S4, methanol fed fermentation; S5, fermentation product collection.

The present invention relates to the use of albumin for use in regulating immune cells in a subject in need thereof. The present invention relates particularly to a composition comprising human albumin for use in regulating immune cells in a subject having systemic inflammatory response syndrome.

The present invention relates to the field of biomedicine, and in particular to a dialysate containing human albumin and glucose and a preparation method thereof. The dialysate of the present invention comprises human albumin, glucose, an osmotic pressure regulator, a buffer, and a stabilizer. Among them, the human albumin content is 4.4% to 12.5% ​​(w / w), and the glucose content is 1% to 2.5%. The present invention provides a low-cost, high-quality dialysate prescription and preparation process, which has good biocompatibility, can avoid the risk of side effects caused by patients during treatment, and plays a key role in the removal of metabolites such as bilirubin and creatinine in patients with renal failure.

The present invention discloses a cell cold storage protection solution and a preparation method and application thereof, and belongs to the field of cell preservation technology. The present invention provides a cell cold storage protection solution, the formula composition includes a compound electrolyte solution, red blood cell preservation solution, heparin sodium, human serum albumin, human platelet lysate and glucose. When preparing the cell cold storage protection solution, mix and prepare it under sterile conditions at room temperature. When the cell cold storage protection solution of the present invention is used to refrigerate a stem cell preparation, the activity of the stem cells is still above 85% after refrigeration for 96 hours, and the growth, proliferation and differentiation potential of the stem cells can be maintained.

The invention belongs to the technical field of biological medicines, and discloses a novel long-acting recombinant human growth hormone fusion protein. The fusion protein is obtained by fusion expression of human growth hormone (hGH) and an anti-human albumin nano antibody (VHH). And the long-acting principle is that the clearance rate is reduced by reversibly and widely combining with albumin with endogenous circulation. The fusion protein has the characteristics that 1, the fusion protein is obtained by fusion expression of two functional domains of hGH and VHH, hGH sequence mutation and complex chemical modification are not involved, the preparation method is simpler, and the sample uniformity is better; 2, in the fusion protein, the N end of the hGH protein has no additional structure, and the VHH functional domain is connected to the C end of the hGH, so that the influence on the activity of the hGH is reduced; and 3, animal efficacy results show that the fusion protein has a long-acting effect.

The invention relates to the technical field of traditional Chinese medicine preparations, and discloses a novel pill for treating diabetes mellitus and a preparation method, and the novel pill comprises the following raw materials in parts by mass: 30-40 parts of Chinese yam, 10-15 parts of poria cocos, 20-25 parts of radix polygonati officinalis, 25-35 parts of guava, 30-35 parts of radix rehmanniae recen, 28-32 parts of radix puerariae, 30-35 parts of corn stigma, 20-30 parts of radix trichosanthis, 25-30 parts of acanthopanax and 20-30 parts of rhizoma polygonati. The preparation method comprises the following steps: S1, screening, cleaning, crushing and homogenizing; s2, performing protein denaturation treatment, performing multi-stage enzymolysis, performing enzyme inactivation and filtration, and performing ultrafiltration membrane separation; s3, carrying out gene fusion to construct a fusion gene expression vector, transferring into pichia pastoris for expression, and purifying; s4, wrapping the target peptide fragment with the human albumin fusion protein; and S5, by taking the hydroxypropyl methylcellulose aqueous solution as an adhesive, molding in a pill pan, pelleting, shaping and drying.

The invention belongs to the technical field of cell therapy, and relates to a serum-free and heterologous component-free NK cell culture medium as well as a preparation method and application thereof. The culture medium comprises a classical culture medium, a proliferation promoting component, vitamins, trace elements, lipids, transferrin, an energy substance and beta-mercaptoethanol, the proliferation promoting component is prepared from human albumin, resveratrol, insulin and hydroxyproline. On the basis of a serum-free and heterologous component-free NK cell culture medium, IL-12, rapamycin, polyinosinic-polycytidylic acid and human AB plasma are additionally added to obtain an activated culture medium, the activated culture medium and an amplification culture medium are jointly used, the culture effect is better, and amplification components IL-2 and human AB plasma are additionally added to obtain an amplification culture medium. Through precise component design, the NK cell in-vitro proliferation efficiency, motility, purity and killing ability are remarkably improved, and key problems existing in a traditional culture medium are efficiently solved.

The present invention discloses an anti-aging composition containing human albumin, a preparation method and application thereof, and relates to the field of cosmetics. The anti-aging composition of the present invention comprises 5 to 20 parts by mass of human albumin, 1.0 to 5.0 parts by mass of ginseng stem and leaf extract, and 0.10 to 0.50 parts by mass of forest frog oil. Human albumin, ginseng stem and leaf extract, and forest frog oil synergize to effectively promote the growth of fibroblasts, have a significant inhibitory effect on collagenase, and have a significant anti-wrinkle effect and good anti-aging effect. The anti-aging composition of the present invention has simple ingredients and a simple preparation method, and has broad application prospects in the field of cosmetics.