28 results about "In utero" patented technology

The invention relates to the technical field of embryo culture platforms, in particular to a microfluidic-AI monitoring-metabolism regulation embryo culture platform and a preparation method thereof.The microfluidic-AI monitoring-metabolism regulation embryo culture platform comprises a microfluidic culture system used for achieving embryo culture, metabolite removal and culture medium replacement; the artificial intelligence monitoring system is used for remotely monitoring the embryonic development state in real time and predicting the embryonic development condition; the metabolism regulation and control system is used for feeding back and optimizing culture conditions online according to an artificial intelligence monitoring result and a metabolite analysis result; the intelligent control module is electrically connected with the micro-fluidic culture system, the artificial intelligence monitoring system and the metabolism regulation and control system, and the micro-fluidic culture system can accurately control the culture environment on the micrometer scale, including temperature, pH value and gas composition parameters. The dynamic fluid environment is closer to the in-vivo physiological environment of the embryo. The system can simulate periodic nutrient supply and metabolic waste removal in the uterus, and provides optimal growth conditions for embryos.

There is provided a computer-implemented method of estimating fetal weight, the method comprising the steps of: a) providing a plurality of ultrasound images comprising image data representative of the anatomical structure and appearance of a head, an abdomen and a femur of a fetus in utero; b) utilising the image data in a first machine learning model comprising one or more neural networks to generate one or more latent space representations of the anatomical structure and appearance of the fetus and one or more predicted measurements of the anatomical structure of the fetus; and c) utilising the one or more predicted measurements and the one or more latent space representations in a second machine learning model to generate an estimated weight of the fetus.

The present disclosure relates to devices and methods for accessing the reproductive tract of an animal, particularly via the animal's vagina, and deploying reproductive material within the uterus. The device and methods of the disclosure may be particularly suited for embryo transfer processes, such as performed in livestock e.g., cattle.

Techniques for determining fetal situs during an ultrasound imaging procedure are disclosed. The techniques can include obtaining an ultrasound image of a fetus in utero. The fetus will include a torso and a spine and the ultrasound image can comprise a circumferential view of the torso of the fetus. The circumferential view can include at least: (i) an outer border of the torso, and (ii) the spine that includes three landmarks arranged in a triangular orientation. The techniques can further include superimposing a fetal overlay based on an alignment instruction corresponding to an alignment between the outer border of the torso and the three landmarks to obtain an augmented reality image of the fetus. The fetal overlay can include a graphical element indicating a left side and a right side of the fetus. The augmented reality image of the fetus can be output on a display of the ultrasound machine.

Disclosed herein is a method for the delivery of prenatal therapeutics, enzyme replacement therapy, or gene therapy to a fetus in need thereof. The method comprises introducing ionizable lipid nanoparticles (LNPs) nanoparticles comprising a therapeutic mRNA composition into the circulation of the fetus in need of treatment such that the ionizable LNPs deliver the therapeutic mRNA composition.

The invention provides a positioning blowdown device for an electrotransfection experiment of an embryo in a mouse uterus, and belongs to the technical field of biomedical appliances. The device comprises an experiment table, a tightening belt is fixedly connected to the top of the experiment table, a magnifying lens is fixedly connected to the top of the experiment table, a normal saline instillation device is fixedly connected to the top of the experiment table, and a dirt storage groove is formed in the top of the experiment table; the sewage discharging assembly is used for collecting sewage generated in the experiment process, and the sewage discharging assembly is connected with the experiment table. By arranging the integrated steel plate structure of the sewage discharge assembly, the guide plate, the supporting part and the sewage collection part, sewage generated in the experiment process can be rapidly and orderly collected by utilizing gradient and flow guide design, the sewage is prevented from flowing around to influence the experiment environment, meanwhile, the sewage discharge assembly is integrally formed by adopting a stamping process, the overall structure is simple, and the cost is low. The manufacturing difficulty is low, and the cost is controllable.

Systems, devices, and methods for configured for directly obtaining fetal oximetry measurements and / or information while a fetus is in utero (negative or 0 station) may be configured to validate that the sensor(s) providing the oximetry information (e.g., optical signals and / or electronic representations of optical signals) correspond to oximetry measurements of fetal, as opposed to maternal, tissue. Additionally, or alternatively, information received via systems, devices, and methods configured for directly obtaining fetal oximetry measurements and / or information while a fetus is in utero may be used to set parameters for the operation of a transabdominal-fetal-oximetry sensor and / or calibrate or adjust data provided by a transabdominal-fetal-oximetry sensor to accurately calculate fetal oximetry information such as fetal hemoglobin and / or tissue oxygen saturation levels.

The utility model discloses a live pig mating insemination device, and particularly relates to the technical field of mating equipment, the live pig mating insemination device comprises an insemination cylinder and an expansion cylinder sleeved outside the insemination cylinder, the expansion cylinder slides outside the insemination cylinder and is detachable, one end of the insemination cylinder is connected with a movable insemination pushing rod in an inserted mode, and the other end of the insemination pushing rod is connected with the expansion cylinder. The other end of the insemination cylinder is arranged to be hemispherical and is provided with a plurality of insemination holes, one side, close to the insemination holes, of the expansion cylinder is provided with a conical portion, the diameter of the conical portion is sequentially decreased, and the joint of the conical portion and the insemination cylinder is sleeved with a spherical cover. According to the utility model, the expansion cylinder is arranged, and lubricating liquid is uniformly scattered and smeared outside the conical part and the expansion cylinder in the insemination process in a pushing manner, so that a lubricating effect is achieved, the expansion cylinder and the insemination cylinder can be conveniently inserted into the uterus, the discomfort suffered by a sow is reduced, and the success rate of mating is improved.

There is provided a method of performing ultrasound examination of a foetus in utero comprising the steps of: a) acquiring a plurality of ultrasound images of the foetus in utero using an ultrasound i

In one aspect, the present disclosure relates to lipid nanoparticles (LNPs) comprising at least one ionizable lipid, at least one helper lipid, cholesterol, at least one conjugated lipid, and at least one nucleic acid cargo (e.g., at least one mRNA and at least one sgRNA). In another aspect, the present disclosure relates to a method of delivering a cargo to the brain of a subject in need thereof. In another aspect, the present disclosure relates to methods of genome editing a mutated gene sequence associated with a disease or disorder in a subject in need thereof. In another aspect, the present disclosure relates to methods of treating a lysosomal storage disease in a subject in need thereof. In certain embodiments, the LNPs of the present disclosure are administrated in utero and / or intracerebroventricularly. In certain embodiments, the subject is embryonic, fetal, neonatal, or perinatal.

The application discloses a culture solution for improving the blastocyst rate and quality of bovine embryos, application and an in-vitro culture method, and relates to the technical field of animal embryo engineering. The application provides a culture solution for in-vitro culture of bovine embryos, which comprises a first culture solution and a second culture solution with different osmotic pressure concentrations. By controlling the osmotic pressure concentrations of the first culture solution and the second culture solution in a suitable range, and by using the first culture solution in the early stage of embryo development and the second culture solution in the late stage of embryo development, the in-vitro culture system can better simulate the environment in the oviduct in the early stage of embryo development and better simulate the environment in the uterus in the late stage of embryo development, so that the osmotic pressure stress of the embryo in the development process is reduced, and the blastocyst rate and the blastocyst quality of the bovine embryo are improved. Compared with a traditional culture system with constant osmotic pressure, the application significantly improves the blastocyst rate by sequential regulation of the osmotic pressure, the total cell number of the obtained blastocysts is more, and the apoptosis index is lower.

There is provided a method of performing ultrasound examination of a fetus in utero, the method comprising the steps of: a) acquiring a plurality of ultrasound images of the fetus in utero using an ultrasound imaging apparatus, the ultrasound images comprising image data representative of the anatomical structure and appearance of a head, an abdomen and a femur of a fetus in utero; b) utilising, on a computing system, a plurality of sets of the ultrasound images in an ensemble machine learning model comprising one or more neural networks to determine an estimated fetal weight and generate an associated uncertainty estimate for the estimated fetal weight; c) providing, based on the uncertainty estimate, a notification to the operator of the ultrasound imaging apparatus in respect of one or more parameters indicative of the accuracy of one or more of the obtained ultrasound images.

The present invention discloses an sgRNA for targeted knockout of APP gene and its application, belonging to the field of animal genetic engineering. By using the CRISPR / Cas9 gene knockout technology, the sgRNA sequence is linked into the PX459 vector, and the PX459-APP targeting vector is transferred into porcine fibroblasts. After resistance screening and PCR sequencing identification, positive cell clones are obtained, and then cloned embryos are obtained through somatic cell nuclear transfer technology; the cloned embryos are transferred into the pig uterus for pregnancy to obtain cloned pigs. The present invention constructs APP gene knockout pigs for the first time, and makes the APP gene not express to obtain ASFV resistance, laying a foundation for the research on breeding against African swine fever.

The apparatus and process described, allows the premature infant to continue the enjoyable in utero experience of sucking and swallowing that is halted upon birth. In particular the infant is afforded the opportunity to practice tasting, sucking, and swallowing on an individualized basis. This oral enjoyment and practice allow for positive association with feedings and provides a sensory-motor experience that may lead to earlier suck, swallow, breathe (SSB) synchrony. The process engages the esophagus and cardiac sphincter to function while the infant is still being fed through the feeding rube. Ultimately, the apparatus and process may also result in earlier weaning from feeding tubes, the reduction of feeding difficulties, and earlier discharge home.

Computer-implemented method of estimating fetal weight using a machine learning model (MLM) 100. Ultrasound images 106 comprise image data representative of the anatomical structure and appearance of

There is provided a method of performing ultrasound examination of a foetus in utero comprising the steps of: a) acquiring a plurality of ultrasound images of the foetus in utero using an ultrasound i

The utility model discloses a newborn big palm type wrapping device which comprises a wrapping cloth bag, the wrapping cloth bag is rectangular, the upper portion of the wrapping cloth bag is open, the left side and the right side of the wrapping cloth bag are each provided with two big palm cloth bags in the shape of a palm from top to bottom, and the end of each big palm cloth bag is provided with five finger bags. The finger bags, the big palm cloth bag and the wrapping cloth bag are internally communicated, traditional Chinese medicine bags are placed in the finger bags, and the wrapping cloth bag, the big palm cloth bag and the finger bags are all made of cloth made of plush materials; by arranging the wrapping cloth bag, the big palm cloth bags and the finger bags, when a newborn is wrapped, the newborn is placed on the wrapping cloth bag, then the big palm cloth bags on the two sides are placed in the front of the body of the newborn in a crossed mode, and therefore a certain safe wrapping feeling is provided for the newborn; and by simulating the palm pacifying characteristic of the parent and the wrapping feeling in the uterus, the pacifying effect on the neonates is improved.

The invention relates to the technical field of neonatal nursing, and discloses a neonatal isolation device capable of simulating sleep inducing of a fetus in the uterus through swinging, the neonatal isolation device capable of simulating sleep inducing of the fetus in the uterus through swinging comprises an isolation box, and the opposite sides of the isolation box are respectively provided with an air supply purification device and an air exhaust purification device; a sleeping mat is arranged in the isolation box, and the side of the sleeping mat is connected with an annular side mat; the distance adjusting assembly is used for adjusting the distance between the two protective pillows; the swinging assembly is used for controlling swinging of the sleeping mat and the edge surrounding mat; the sleeping cushion swings to drive the flapping cushion to flap relative to the protective pillow. According to the newborn isolation device capable of simulating sleep inducing of the fetus in the uterus through swinging, the mattress swings to drive the beating pad to beat relative to the protective pillow, the newborn is beat to sleep through the beating pad, the wrapping feeling of the newborn during sleep is further improved, the sleep quality of the newborn is improved, and the sleep quality of the newborn is improved by combining beating inducing of the beating pad and swinging of the mattress. And the sleep inducing effect of the newborn is improved.

A safe incubator cover for newborns comprises an incubator and an incubator cover body, the incubator cover body is divided into a front face and a back face, the middle of the incubator cover body is an anti-skid area, the incubator cover body is divided into a left side cover curtain and a right side cover curtain with the anti-skid area as a boundary, anti-skid strips are distributed in the middle of the back face of the anti-skid area, and first pasting pieces are arranged on the anti-skid strips; third sticking pieces are arranged at the upper end and the lower end of the front surface of the right side cover curtain; a thin cotton cloth layer, a sound insulation cotton layer and a shading material layer are sequentially arranged on the incubator cover body from the outer layer to the inner layer. The shading and noise reduction effects on the incubator are achieved, a dark environment in the uterus is created, the sense of safety of the newborn is enhanced, and sleeping is promoted; the incubator cover main body can be folded and unfolded in multiple directions, so that nurses can carry out nursing operation conveniently, and vital signs of newborns and the activity state in the incubator can be observed conveniently; the incubator cover body is placed in the middle of the top of the incubator through the anti-slip strips and is not prone to slipping off.

Embodiments described herein provide enhanced methods, techniques, and methods of treatment for consistently correcting facial asymmetries caused by gestational position of a fetus in utero using an improved method of treatment based upon facial asymmetry grading. With SSM, the patient is evaluated to determine whether their facial asymmetries are a result of FLT; and assuming so, a determination is made as to the severity of the asymmetries based upon the asymmetry severity scale guide. Based upon the determined severity and type of asymmetry, the injector performs an SSM plan that consistently corrects such asymmetries by injecting more filler to the more contoured side of the face as opposed to the heavier side, and in consistent amounts and to certain locations.

The utility model relates to the technical field of toys, in particular to a baby sleep aiding device. The infant pacifying device comprises a supporting and limiting assembly, the two sides of the supporting and limiting assembly are each sleeved with a soft bag, the pacifying assembly is matched with the soft bags to form a wrapping space, and the pacifying assembly is used for alternately making contact with the body of an infant to form the wrapping space. According to the utility model, a baby can have a wrapping feeling before falling asleep, the baby feels protected, the sense of safety is improved, the pacifying assembly continuously contacts with the body of the baby, the condition that the baby slightly changes a knife when being in the uterus of a mother is further simulated, the baby can feel relieved and comfortable, and the falling asleep effect is improved; moreover, after the infant falls asleep, the supporting and limiting assembly keeps limiting the periphery of the infant, the situation that the infant turns over and shakes after falling asleep and is separated from the pacifying assembly is avoided, meanwhile, the periphery of the infant is protected, the infant is prevented from being pressed by an adult during accompanying sleep, and safety is improved.

The invention discloses a construction method and application of a DFNB1 type deafness mouse model with GJB2 gene frameshift mutation. The construction method comprises the following steps: (1) designing two sgRNA plasmids on a No.2 exon of a mouse Gjb2 gene; (2) screening out mouse embryonic stem cells of which the Gjb2 gene carries a c.299300delAT mutation site; (3) forming a recombinant embryo, transplanting the recombinant embryo into the uterus of the pregnant female mouse, and waiting for pregnancy and farrowing to obtain an F0-generation mouse; and (4) screening the F0 generation mice in the step (3) to obtain the mouse model carrying the c.299300delAT homozygous point mutation of the GJB2 gene. The method has the advantages that the problem of mouse embryo metaphase energy supply disorder lethal caused by Gjb2 homozygous mutation is solved, the embryo survival rate is increased, and the bottleneck of embryo lethal in model construction of a traditional gene editing technology is broken through.

The invention is a training material (100) of pregnancy calculation with fundal height, developed to be used in determining the developmental week of the fetus for the evaluation of the health of the fetus in the womb and for the early detection of problems that may occur, showing where baby should be in the abdomen according to its development, wherein it comprises a diagram on which the mother's xiphoid protrusion (lower part of the breastbone), umbilicus (belly button) and symphysis pubis (pelvis) are marked, and a body (110) containing a chart of the fundal height according to the gestational age at the bottom of this diagram; for the demonstration of the gestational week, a moving week chart (120) positioned at the back of the body (120) with numbers indicating the gestational week marked thereon.

The present invention relates to an antibody-based molecule for use in the treatment of a disease or condition resulting from a genetic defect in a fetus wherein the antibody is administered to the mother of said fetus.

The present disclosure provides a non-invasive method for early diagnosis of a placental pathology comprising an abnormal formation or arrangement of a placenta in a uterus of a mammalian female subject during pregnancy. Early diagnosis can lead to an improved maternal outcome. The method comprises selectively purifying from plasma of maternal blood a population of small extracellular vesicles (small-EVs) expressing a placenta-specific surface biomarker. The extracellular vesicles comprise micro-RNA cargo. A cargo profile for the small EVs is determined by extracting RNA from the purified population of small EVs. Expression of small non-coding RNAs comprising one or more micro RNAs (miRNAs) encapsulated by the purified population of exosomes is then identified and quantified. The miRNA profile of the placenta specific EVs is then compared to the miRNA profile of a healthy control of the same approximate gestational age.

The invention relates to a piston type embryo transfer tube, which comprises a lead-in tube part, a catheter part and a push-in part. The right end of the lead-in tube part can be pre-bent to a certain angle. The catheter part contains a catheter and a supporting tube sleeved on the left side of the catheter, and the right end of the catheter extends outwards relative to the supporting tube. The push-in part comprises a push rod, a piston body and a handle end respectively arranged at the two ends of the push rod. The piston body extends into the catheter cavity of the catheter, and the outer wall of the piston body is in contact with the inner wall of the catheter cavity. The piston body can be pushed by the push rod to approach the right end port of the catheter cavity. The push rod is formed with an elastic deformation section, so that the piston body can be bent relative to the push rod. The patent can ensure that the embryo is fully pushed into the uterus during embryo transfer, and it is not necessary to check whether the embryo is retained in the catheter in subsequent inspection. The time of the lead-in tube staying in the uterus can be shortened, which helps to reduce the stimulation of the uterus during embryo transfer and helps to improve the pregnancy rate.

The present disclosure provides a non-invasive method for early diagnosis of a placental pathology comprising an abnormal formation or arrangement of a placenta in a uterus of a mammalian female subject during pregnancy. Early diagnosis can lead to an improved maternal outcome. The method comprises selectively purifying from plasma of maternal blood a population of small extracellular vesicles (small-EVs) expressing a placenta-specific surface biomarker. The extracellular vesicles comprise micro-RNA cargo. A cargo profile for the small EVs is determined by extracting RNA from the purified population of small EVs. Expression of small non-coding RNAs comprising one or more micro RNAs (miRNAs) encapsulated by the purified population of exosomes is then identified and quantified. The miRNA profile of the placenta specific EVs is then compared to the miRNA profile of a healthy control of the same approximate gestational age.

Compositions and methods for fetal or in utero delivery of active agents are provided. The compositions are most typically administered intravenously via the vitelline vein, umbilical vein, or directly into the amniotic cavity of a pregnant mother. Fibroblast growth factor is to correct structural defects of neural tissue.