The invention discloses a tissue cultivating method of choerospondias axillaries. The method includes the steps of taking axillary buds of choerospondias axillaries, disinfecting outer surfaces, cultivating the axillary buds in an MS medium, screening and disinfecting the axillary buds, putting the disinfected axillary buds in an inducing medium to be cultivated at a temperature of 10 DEG C to 12 DEG C under the luminance of 1800 LX 8 hours per day for 6 weeks, shifting the axillary buds into a subculture medium to be cultivated to continue multiplication and cultivation for 4 weeks, and shifting the buds into a root medium to be cultivated to conduct rooting cultivation for 4 weeks. By means of the method, the genders of choerospondias axillaries seedlings can be controlled and cultivated in an oriented mode by recording the genders of collected female parents, the method is free of season limits, keeps the biological characteristics of original plants to the maximum extent, and can well prevent the browning in the cultivation process, the reproduction speed is high, and the rooting rate is 98% or higher.