Acellular Bone Matrix Composite Material with Local Anticoagulation and Cell Capture and Preparation Method

A decellularized bone matrix and composite material technology, applied in the field of local anticoagulant and cell-captured acellular bone matrix composite materials and preparation, can solve the problems of difficult guarantee of effect, limited transportation, limited distance, etc., to prevent blood clots Formation, extended penetration distance and time, low biological properties

Inactive Publication Date: 2016-02-03
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the tissue engineered bone constructed in the bioreactor has osteogenic effect when implanted in the body, there is no direct connection with the blood circulation in the recipient area at the initial stage of implantation, and the exchange between various substances produced by the survival and metabolism of the seed cells and the recipient is still carried out by osmosis , the distance is limited, and the effect is difficult to guarantee; on the other hand, tissue damage wounds or surgical incisions will activate the body's blood coagulation system to produce a protective response, and the graft surrounded by blood clots in the bone defect and the delivery of internal nutrients and oxygen are extremely limited

Method used

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  • Acellular Bone Matrix Composite Material with Local Anticoagulation and Cell Capture and Preparation Method
  • Acellular Bone Matrix Composite Material with Local Anticoagulation and Cell Capture and Preparation Method
  • Acellular Bone Matrix Composite Material with Local Anticoagulation and Cell Capture and Preparation Method

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Prepare the decellularized bone matrix (HeparinchitosancoatedACBM, HC / ACBM) of composite heparin-chitosan

[0036] 1) Take a part of the decellularized bone matrix material (pork bone or human bone) and soak it in a heparin solution (acetic acid buffer solution with pH=4.0) with a concentration of 0.05%-0.1% by mass, and the soaking time is 1.5-3 hours, after soaking, rinse with acetic acid buffer solution with pH=4.0 for 3 times, each time for 3-8 minutes; after soaking for 1.5-3 hours, rinse with pH=4.0 acetate buffer solution for 3 times, each time for 3-8 minutes; A total of 12 to 25 minutes or so. The acellular bone matrix collagen is exposed and positively charged, and heparin is negatively charged under the condition of PH=4.0 acetate buffer, and the collagen fibers on the surface of the acellular bone matrix material are positively charged and heparin (under specific pH conditions) is charged. Negatively charged macromolecules combine by charge intera...

Embodiment 2

[0040] Example 2 detection

[0041] 1. Material morphology and structure

[0042] The results of scanning electron microscopy showed that the material still maintained the natural structure of bone tissue before and after the composite; there was no significant difference in the mesh size and mesh ratio. connected (see figure 1 ). After compounding, it can be seen that the surface of the material becomes smooth ( figure 1 : show that coating is formed); X-ray diffraction photoelectron spectroscopy (XPS) results show that with the increase of composite coating, the sulfur element on the surface of the material increases (only the sulfur element contained in heparin can be detected in the material), i.e. Contains more heparin (see figure 2 ). Infrared spectrum analysis shows that the heparin in the coating composition is negatively charged and the positive charge in chitosan is combined through electrostatic interaction to form a composite coating. The base absorption pea...

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Abstract

The invention relates to an acellular bone matrix composite with a partially anti-freezing function and a cell capturing function and a preparation method thereof, and the bone matrix material is formed by assembling sulfuric acid polysaccharide with an anti-freezing function and chitosan or fibronectin onto the surfaces of an acelluar bone matrix. The preparation method of the acellular bone matrix composite comprises the following steps: firstly, preparing the acellular bone matrix material; and then assembling the sulfuric acid polysaccharide and other materials with the anti-freezing function and the chitosan or the fibronectin onto the surfaces ( the inner surface and the outer surface) of the acelluar bone matrix in a layer upon layer mode under an intermolecular electrostatic interaction or ligand interaction method so as to form a controlled-release coating. The composite retains the natural structure, biological characteristics and low immunogenicity of bone tissues, and simultaneously the composite coating has favorable anti-freezing function and seeded cell capturing function. The composite serving as a material for filling bone defects or a tissue engineering bone bracket has obvious function of promoting vascularization, and has scientific, reasonable, simple, convenient and feasible processing and manufacturing.

Description

technical field [0001] The invention relates to an implant material required for bone defect and nonunion repair and a scaffold material for constructing tissue engineered bone, in particular to a decellularized bone matrix composite material with local anticoagulation and cell capture and a preparation method thereof. Background technique [0002] Clinically, bone defects caused by various reasons are very common, and its repair has always been a difficult problem in orthopedics. The ideal method of bone defect repair is constantly being explored. The regenerative repair method of tissue engineered bone brings new hope to the repair of bone defects. After 20 years of development, abundant results have been achieved in three main research areas. However, after the seed cells and scaffold complex are implanted into the body, angiogenesis takes several days, and the cells can tolerate hypoxia for several hours; the oxygen and nutrients required for cell survival can only be su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/34A61L27/36A61L33/12A61L33/08A61L33/10
Inventor 张波孙新君王正国张世昌张连阳周继红任明亮朱佩芳刘大维杨在亮
Owner INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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