A kind of convenient preparation method of high-performance collagen gel

A collagen gel, high-performance technology, applied in the field of biomedical material preparation, can solve the problems of long time consumption, cytotoxicity of cross-linking agent, collagen denaturation, etc. Effect

Active Publication Date: 2019-04-16
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It was found that the EDC of 125mmol / L was cross-linked, and the elastic modulus of the collagen gel obtained after 3 cycles could reach 3730Pa, which was 85 times that of the self-assembled collagen gel (Hideki Mori, Kousuke Shimizu, Masayuki Hara. Dynamic viscoelastic properties of collagengels with high mechanical strength), although this method can obtain collagen gels with high mechanical strength, the whole process takes about 7 days, which is time-consuming and complicated. Chinese invention patent CN104231288A uses 1-50kGy gamma rays or high-energy electron beam radiation to crosslink collagen aqueous solution or its mixed aqueous solution with other natural polymers, water-soluble synthetic polymers and vinyl monomers to prepare low-density collagen hydrogel , and then place the low-density collagen gel at 30-40°C for 2-72h to cause three-dimensional shrinkage, and the compressive modulus of the prepared collagen gel is 2-5 times higher than that of the untreated collagen gel , the performance improvement of collagen gel obtained by this method is not obvious and other polymer materials will be introduced
[0005] The preparation of high-performance collagen gels faces problems such as collagen denaturation caused by heat treatment, insufficient mechanical properties of cross-linked collagen gels, cumbersome preparation process, high requirements for process conditions, and cytotoxicity of residual cross-linking agents.

Method used

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  • A kind of convenient preparation method of high-performance collagen gel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The freeze-dried undenatured natural bovine collagen sponge was dissolved in 10 mmol / L disodium hydrogen phosphate / sodium dihydrogen phosphate buffer (NaCl concentration was 120 mmol / L) at pH 6.8 under an ice-water bath to obtain a concentration of 8 mg / mL Uniform cowhide collagen solution, take 3mL cowhide collagen solution and place it in a 35mm mold, keep the temperature at 37°C for 6h, then soak the prepared collagen gel with 240mL ethanol solutions with volume concentrations of 10, 20, and 30% for 30min , and finally the collagen gel was treated with 240 mL of ethanol solution with a volume concentration of 80% for 24 hours, and the solution was changed every 4 hours to prepare a high-performance cowhide collagen gel. With a rheometer at 2% strain and at a temperature of 20 ° C, the elastic modulus of the untreated natural cowhide collagen gel at a frequency of 1 Hz is 241.21Pa, and the elastic modulus of the high-performance collagen gel is 4365.82Pa, which is The...

Embodiment 2

[0029] The undenatured natural cowhide collagen sponge of freeze-drying is dissolved in the Tris / HCl buffer solution (NaCl concentration is 150mmol / L) of 50mmol / L of pH 7.6 under ice-water bath to obtain the uniform cowhide collagen solution that concentration is 3mg / mL, take Put 3mL of cowhide collagen solution in a 35mm mold, keep the temperature at 30°C for 2h, then soak the prepared collagen gel with 60mL of ethanol solution with volume concentration of 10, 15, and 25% for 30min, and finally gel the collagen The gel was treated with 60 mL of isopropanol solution with a volume concentration of 60% for 12 hours, and the solution was changed every 1 hour to prepare a high-performance bovine collagen gel. With a rheometer at 2% strain and at a temperature of 20 ° C, the elastic modulus of the untreated natural cowhide collagen gel at a frequency of 1 Hz is 128.46Pa, and the elastic modulus of the high-performance collagen gel is 1479.15Pa. The modulus of elasticity increased b...

Embodiment 3

[0031] The freeze-dried undenatured natural pigskin collagen sponge was dissolved in 12 mmol / L disodium hydrogen phosphate / citric acid buffer (NaCl concentration was 100 mmol / L) at pH 7.2 under an ice-water bath to obtain a uniform concentration of 5 mg / mL. Pigskin collagen solution, take 3mL pigskin collagen solution and place it in a 35mm mold, keep the temperature at 35°C for 3h, then soak the prepared collagen gel in 150mL ethanol solutions with volume concentrations of 15, 20, and 30% After 30 minutes, the collagen gel was finally treated with 150 mL of tert-butanol solution with a volume concentration of 70% for 18 hours, and the solution was changed every 3 hours to prepare a high-performance pigskin collagen gel. The elastic modulus of the untreated natural pigskin collagen gel measured at a frequency of 1 Hz at 2% strain and a temperature of 20°C with a rheometer is 215.28Pa, and the elastic modulus of the high-performance pigskin collagen gel is 4116.52 Pa, its modul...

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Abstract

The invention discloses a convenient preparation method for a high-performance collagen gel. The method is characterized by comprising the following steps: placing a self-assembled collagen gel into an alcohol solution and performing gradient dewatering, thereby replacing the solvent in the collagen gel with the alcohol solution, and then contracting the size of the collagen gel at a certain degree, thereby acquiring the high-performance collagen gel. The whole technological operation is simple and the time consumption is less and is about 18-34h; the collagen cannot denature under the effect of the technical temperature; the elasticity modulus of the prepared high-performance collagen gel can reach up to 11-53 times of that of the untreated collagen gel and can be widely applied to the fields of tissue repair, drug sustained release and clinical medicine.

Description

technical field [0001] The invention relates to a convenient method for preparing high-performance collagen gel, which belongs to the field of preparation of biomedical materials. Background technique [0002] As the main component of the extracellular matrix, collagen is the most abundant protein in animals. It exists widely in connective tissues of mammals such as skin, blood vessels and tendons, protecting and supporting animal organs. As a natural polymer material, due to the natural triple helix structure of collagen, collagen has the characteristics of low immunogenicity, biocompatibility, biodegradability and hemostatic repair function, and it is used in drug delivery, tissue engineering, clinical medicine, etc. and other fields have extensive research and application. [0003] Gel is a semi-solid material composed of a polymer with a three-dimensional network structure and a medium filled in the gap between polymer chain segments. Because it has both solid and liqui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/11C08J3/09C08L89/00
CPCC08J3/095C08J3/11C08J2389/00C08L89/00C08L2203/02
Inventor 李国英沈里瑞徐松成刘文涛
Owner SICHUAN UNIV
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